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All Journal Jurnal Hortikultura Indonesia (JHI) AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Seminar Nasional Lahan Suboptimal Akta Agrosia BIOVALENTIA: Biological Research Journal

Mery Hasmeda

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Author-ID : 278198

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Other

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Pengujian Berbagai Varietas Padi (Oryza sativa L.) terhadap Kondisi Cekaman Fe2+ Di Lahan Pasang Surut Dirgasari, Karterine Dewi Endah; Hasmeda, Mery; Harun, Umar
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 3 No 1 (2019): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Pengembangan budidaya tanaman di lahan pasang surut memiliki kendala cekaman Fe2+ yang menjadi faktor pembatas pertumbuhan dan produksi tanaman. Penelitian ini bertujuan untuk mempelajari karakteristik pertumbuhan dan kemampuan produksi dari varietas padi di lahan pasang surut serta untuk mendapatkan varietas yang toleran terhadap cekaman Fe2+ di lahan pasang surut. Metode yang digunakan dalam penelitian ini yaitu Rancangan Acak Kelompok (RAK) tunggal dengan 20 perlakuan varietas yang diulang sebanyak 3 kali. Uji lanjut menggunakan uji BNT 5%. Perlakuan yang diaplikasikan adalah 20 varietas padi yang meliputi varietas Towuti, Siam Unus, Mashuri, Mekongga, Ciherang, Cilamaya Muncul, Pokalli, IR 64, Siak Raya, Inpari 30, Inpara 1, Limboto, Inpara 3, Inpara 4, Inpara 5, Inpara 6, Inpara 7, Inpara 8, Inpara 9 dan Awan Kuning. Hasil penelitian menunjukkan bahwa panjang akar, jumlah anakan, bobot gabah per plot dan bobot basah malai berpengaruh sangat nyata terhadap parameter yang diamati. Varietas Mashuri, Mekongga, Towuti, Siam Unus dan Awan kuning memiliki tingkat toleran yang tinggi pada lahan yg cengkaman Fe nya tinggi dilahan pasang surut dibanding dengan varietas lainnya.

BC2F2 LOCAL RICE GENOTYPES FIELD TEST AT NON-TIDAL SWAMP IN SOUTH SUMATRA PROVINCE Fitri, Milia; Hasmeda, Mery; Suwignyo, Rujito A.
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Non-tidal swamp is one of suboptimal land available to increase rice production in Indonesia. The problems is stagnant flooding during vegetative growth phase of the crop. Therefore, it is important to have rice varieties that tolerant flooding. Crossing and selection have been conducted to obtaine BC2F2 genotype from local swamp rice varieties, i.e. Siam, Pegagan and Pelita Rampak. This research was aimed at knowing the growth and production of several genotypes BC2F2 and their parental varieties, Siam, Pegagan and Pelita Rampak. The research was conducted in non-tidal swamp in Village Sako, sub-district Rambutan, District Banyuasin, South Sumatra from October 2017 to March 2018. Rice genotypes have been used BC2F2 Siam, BC2F2 Pegagan, and BC2F2 Pelita Rampak, and their parental varieties i.e. Siam, Pegagan, and Pelita Rampak. The results showed that genotype BC2F2 had vegetative and generative characters different from those of Siam, Pegagan, and Pelita Rampak. The production of the tested rice varieties was high, ranged from 4.52 to 7.95 tonnes per hectare. The best of each genotypes planted in the first season were SM 2, PGG 4, and PLR 3. While the best BC2F2 of each genotypes planted in the second season were SM 2, PGG 3, and PLR 3.

CANDIDATE GENES IDENTIFICATION OF OIL PALM (Elaeis guineensis Jacq.) INTEREST CHARACTERS USING PUBLISHED DATABASE Sarimana, Upit; Putro Priadi, Dwi; Hasmeda, Mery; Erika, Pratiwi; Herrero, Javier; Santika, Baitha; Wendra, Fahmi; Sembiring, Zulhermana; Asmono, Dwi
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Palm oil has supplied more than 30% of vegetable oil consumption worldwide. Rising demand has pushed oil palm plantations to increase the yield. It is well known that genetic has played a significant role in phenotypic performance. Moreover, in recent years, genomic data has emerged tremendously. Unfortunately on the gene related to oil palm yield. Therefore, a preliminary study to classify and select oil palm candidate genes associated with characteristic by scanning existing genes in oil palm or other in-silico species were conducted. Based on Blast2Go results, 22 genes related to oil biosynthesis, two specifically related to fruit number and fruit weight were analysed. Furthermore, 19 candidate genes were able to amplify.

MORPHOLOGICAL CHARACTERISTICS AND PLEASANT RELATIONSHIP BETWEEN CROSSING OF BLACK RICE ACCESION WITH INPARA 5 THAT CONTAINING SUB-1 GENES Gladysha, Utary; Halimi, E.S; Hasmeda, Mery; Sarimana, Upit
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

In South Sumatra the cultivation of black rice is uncommon. This is because of the lack of available land for planting which can be used to grow local black rice. Since more farmers dominate the white rice or brown rice cultivation. One effort that can be made to increase the area of local black rice planting areas is to use South Sumatra's vast swampy land. Related to the previous study, this research will be carried out by testing the growth of F1 seeds crossed by accession to high productivity local black rice and nutrition with marinated resistant Inpara 5 varieties. In quantitative parameters, the maximum average value of it was shown at the age of 12 MST plants that IM.6 plants at 145.08 as the highest average value of the crossing results. IM.6 was the highest median value with 144.5. The maximum standard deviation value was 5.06 for IT.11 plants, and the minimum value being 141.4 for IM.6 forms and 150.0.6 for IM.6 plants. The qualitative parameters consist of plant shape, stem color, leaf color, and flag leaf are seen when the plant is 6 MST or in the vegetative phase. Done by describing plants from 5 sample plants per randomly selected population. From the result of this research, there is no significant differential between accession and parental.

MORPHOLOGICAL CHARACTERISTICS OF RICE VARIETIES ON IRON STRESS IN NUTRIENT CULTURE MEDIA Oktatora, Emilia; Agus Suwignyo, Rujito; Hasmeda, Mery; Damayanti, Rosa
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Decreased rice productivity due to high iron concentrations especially in the vegetative phase, this causes the morphological character of rice to be affected until the metabolic process is disrupted. This study used 6 varieties, namely Batu Tegi, Inpara 9, Situ Patenggang, Inpara 8, Hawarabunar and Inpago 8, with iron concentrations of 0 and 250 ppm. The seeds were germinated for 3 days, then planted in Kimura B 10% nutrient culture media for 9 days, for 2 weeks then each week the media was changed to 100% Kimura B solution with the addition of 250 ppm iron concentration which was sampled every week until the 35th day. The results of the analysis of variance of the variables of plant height and root length showed that the combination Inpara 8-01 was the best combination and had a significant effect compared to other combinations with an average value of 61.31 cm and 30.77 cm. In the variable number of leaves the best average value was found in the combination of Batu Tegi-01 and Inpara 9-01 which had a significant effect compared to other treatments with an average value of 5.87 strands and 9.60 strands, respectively. On the stem dry weight variable, the combination of Hawarabunar-250 ppm Fe had a significant effect on other treatment combinations with the highest value of 0.17 g and the lowest value of treatment combination Inpara 9-250 ppm with a value of 0.08 g. Based on the results of treatment with 250 ppm Fe stress on plant morphological characters, the Hawarabunar variety had the best response in tolerating Fe stress, while the Batu Tegi variety was the lowest in tolerating Fe stress.

THE EFFECT OF CULTURE MEDIA TYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION OF OIL PALM (Elaeis guineensis Jacq) PISIFERA TYPE Sulaksono, Gogoh; Hasmeda, Mery; Hanum, Laila; Wendra, Fahmi; Santika, Baitha; Asmono, Dwi
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Oil palm has a high economic value since it is one of the highest vegetable oil-producing plants compared to other oil-producing plants. The provision of good planting material for oil palm plantations is one of the determining factors to increase productivity. The parent Pisifera type of oil palm is important to develop the high-yielding oil palm Tenera varieties. The provision of oil palm material could be done through generative system with seeds and also could be done through vegetative approaches using tissue culture techniques. It aims to get plants that are genetically the same as their parents (true to type). The purpose of this study was to see the effect of different type of tissue culture media and plant growth regulators (PGRâ€™s) on the callus induction stage of Pisifera type oil palm. The results show that the treatment using Murasige and Skoog (MS) culture media is able to give a better effect when compared to the use of Eeuwens and Blake (Y3) basic media. While the use of PGR treatment (H6 = NAA 6 mg/l + 2.4-D 0.5 mg/l) showed a callus growth percentage of 76%, which was better than (H1 = Kin 0.1 mg/l + 2.4-D 100 mg/l) by 42%.

Evaluasi Hasil Persilangan Tanaman Padi Beras Hitam dengan Varietas Inpara 5 pada Lahan Terendam di Sumatera Selatan Nabil, Shiva Naizak; Pratiwi, Shafira; Merlita Dewi, Ana Tasya; Fadhilah, Maratul; Ester Sitio, Rimma; Silalahi, Yose Armando; Hasmeda, Mery
Seminar Nasional Lahan Suboptimal Vol 12, No 1 (2024): Vol 12, No 1 (2024): Prosiding Seminar Nasional Lahan Suboptimal ke-12 “Revital
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Nabil, S. N., Pratiwi, S., Dewi, A. T. M., Fadhilah, M., Sitio, R. E., Silalahi, Y. A., & Hasmeda M. (2024). Evaluation of the hybridization results of black rice with inpara 5 varieties on waterlogged lands in South Sumatra. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-12 Tahun 2024, Palembang 21 Oktober 2024. (pp. 103–117). Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Black rice had the disadvantages of high habitus, low productivity, and no soaking resistance. The purpose of this study was to determine the yield potential of black rice crosses with Inpara 5 varieties, especially on submerged land in South Sumatra. This field practice was carried out in South Sumatra Province. Field practice research on plant production was selected from the results of submerged stress tests, in testing plant growth and production the method used was Randomize Group Design (RDG). The results of research on submerged stress testing include the response of several black rice accessions to submerged stress, with measurements of parameters such as plant height, number of tillers, and chlorophyll levels before and after submersion for 14 days of submision. The results showed that plant height increased significantly from an average of 57.84 cm at 20 DAT (Days After Trasplatnting) to 83.93-96.00 cm at 49 DAT with accession I recording the highest height. The number of tillers decreased after submergence stress, with the highest average at 2.90 before submergence and the lowest at 1.29 at 49 DAT, while chlorophyll levels showed little change. In the final observation, accession H showed superior results in production parameters, such as number of panicles, total grain weight, and number of full grains, making it the most productive accession compared to others. In general, all accessions used in this study have the potential to be developed into new varieties of submergence-tolerant black rice. Based on the results of the study, it is suggested that better plant breeding can be done on black rice that shows the best resistance to submergence stress.

Rice Seedling Resistance due to Seed Priming and Seeding Density under Submergence Stress Condition Sulaiman, Firdaus; Agus Suwignyo, Rujito; Hasmeda, Mery; Wijaya, Andi; Irmawati, Irmawati
Akta Agrosia Vol 25 No 2 (2022)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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The study of rice seedling resistance due to seed priming and seeding density under submergence stress condition was carried out to evaluate the effect of seed priming and also to estimate proper seeding population density to improve rice seedlings vigor under submergence stress condition. The เว็บสล็อต | R4shub experiment was conducted in tidal freshwater swamp of Sako in Rambutan District, Banyuasin Regency (3.067° S, 104.8616° E). Randomized block design was used where the seeds were soaked into ZnSO4.7H2O solution as priming treatment, and then sown with three different seeding rates: 30, 40 and 50 kg ha-1. Three cultivars consisted of Inpara 3, Inpara 5 and Ciherang were tested. The observation was carried out until 4 weeks after sowing to measure the seedling height, shoot dry weight, carbohydrate content in stem, and leaf chlorophyll. Results showed that all treatments indicated insignificant effect to all parameters. However, the highest result from all parameters was obtained from priming treatment. The higher seeding density the lower dry weight, carbohydrate and chlorophyll content. All three tested cultivars, Inpara 3 gave the best performance.

The The Effectiveness of Different Sterilant Combination on Citrullus lanatus L. In Vitro: Uji Efektivitas Kombinasi Bahan Sterilan yang Berbeda pada Citrullus lanatus L. secara In Vitro Indah Lestari, Laras; Irmawati; Negara, Zaidan P.; Hasmeda, Mery; Sulaiman, Firdaus; Sulistyaningsih, Lidwina N.; Marlina
Jurnal Hortikultura Indonesia (JHI) Vol. 16 No. 2 (2025): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.16.2.125-135

Watermelon plants require consistent environmental conditions throughout their growth period. Tissue culture serves as a solution in plant propagation techniques that can control the environmental conditions for watermelon growth. This study aims to determine the appropriate sterilization method and explant type for watermelon plant propagation using tissue culture techniques. The research was conducted from June to August 2022 at the Tissue Culture Laboratory of the Faculty of Agriculture, Sriwijaya University. This study used a Complete Randomized Factorial Design (CRFD) with two factors: six treatments of different sterilization agent sequences for explants, including Benomyl 0.2% for 3 minutes, detergent for 3 minutes, HgCl₂ 0.03% for 10 minutes, and 70% alcohol for 45 seconds, and four types of explants: radicle, hypocotyl, plumule, and cotyledon, with two replicates of five experimental units each. The results showed that sterilization factors and explant types had a significant effect on the percentage of contaminated explants and indicated a decrease in contamination in the treatment of 0.2% benomyl for 3 minutes, detergent for 3 minutes, 0.03% HgCl2 for 10 minutes, and 70% alcohol for 45 seconds on cotyledon explants. Keywords: browning, contamination, tissue culture, watermelon sprout

The Effect of Shade, KNO3, Bulbil Conditions on The Shoot Emergence and Porang Growth nurshanti, Dora fatma; Lakitan, Benyamin; Hasmeda, Mery; Ferlinahayati; Zaidan, Zaidan; Susilawati, Susilawati; Rofiqoh, Purnama Ria
BIOVALENTIA: Biological Research Journal Vol. 11 No. 2 (2025)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/biov.11.2.2025.386

Porang cultivation can be carried out using three planting materials, namely true seeds, bulbils, and cormel, these three planting materials experience dormancy. Potassium Nitrate Solution (KNO3) is a chemical compound that is effective in breaking the dormancy of some plant seeds.This study aims to the effect of shading, soaking with KNO3 and bulbil conditions on the emergence of shoots and the growth of porang plants. The research was conducted in September 2021 to November 2021. The design used was split split plot using three factors, shading (N) as the main plot, namely N0: without shade, N1: 45%, N2: 55%, N3: 80%, KNO3 concentration (K) as subplots, namely K0: Without immersion KNO3, K1: 1500 ppm, K2: 3000 ppm, K3: 4500 ppm and bulbil conditions (D) as sub-sub plots, namely D0: Dormant and D1: Mentis. The data collected included the percentage of shoots emergence, time of shoot emergence, broken petiole sheath, leaf open fully, shoot height, number of shoots, number of leaves, petiole length, petiole diameter, midrib length, leaf width, leaf area and leaf thickness. The results showed that shading 55% and bulbil mentis accelerated the emergence of shoot at 26 DAP and 26.9 DAP, respectively. Soaking in KNO3 solution did not accelerate the emergence of shoots. The emergence of shoots affects the time of petiole sheath break and the leaves open fully, the faster the emergence of shoot, the faster the petiole sheath breaks, the faster the leaves open fully. Shade 80% increases the growth of porang plants with the longest leaf petiole of 22.6 cm; petiole diameter 5.5 mm; midrib length 10.34 cm; the width of the leaflets is 4.7 cm and the leaf area is 183.2 cm², except for the thickness of the leaves.

Co-Authors ANDI WIJAYA Benyamin Lakitan Damayanti, Rosa Dirgasari, Karterine Dewi Endah Dwi Asmono Dwi Putro Priadi Erika, Pratiwi Ester Sitio, Rimma Fadhilah, Maratul Ferlinahayati Ferlinahayati Firdaus Sulaiman, Firdaus Fitri, Milia Gladysha, Utary Halimi, E.S Herrero, Javier Indah Lestari, Laras Irmawati Irmawati Irmawati Laila Hanum Marlina Merlita Dewi, Ana Tasya Nabil, Shiva Naizak Negara, Zaidan P. Nurshanti, Dora Fatma Oktatora, Emilia Pratiwi, Shafira Rofiqoh, Purnama Ria Rujito A. Suwignyo, Rujito A. Rujito Agus Suwignyo Santika, Baitha Sarimana, Upit Sembiring, Zulhermana Silalahi, Yose Armando Sulaksono, Gogoh Sulistyaningsih, Lidwina N. Susilawati, Susilawati Umar Harun, Umar Wendra, Fahmi Zaidan, Zaidan

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