African catfish (Clarias gariepinus) is one of the freshwater fish commodities with high economic value. The high cost of feed in catfish culture has encouraged the use of alternative feed ingredients that are more economical, one of which is chicken intestine. This study aimed to analyze the effect of different chicken intestine dosages on the growth and survival rate of African catfish, determine the optimum dosage, and evaluate feed cost efficiency. The study was conducted for 30 days at the Wet Laboratory, Faculty of Fisheries, Universitas 45 Mataram, using a Completely Randomized Design (CRD) consisting of four treatments and three replications. The treatments were P1 (3% chicken intestine), P2 (5% chicken intestine), P3 (7% chicken intestine), and P4 (commercial pellet 5% feed as control). Data were analyzed using Analysis of Variance (ANOVA) followed by Duncan’s Multiple Range Test at a 5% significance level. The results showed that different dosages of chicken intestine significantly affected the growth of African catfish. ANOVA indicated that the calculated F-value (37.45) was higher than the F-table value (4.07) with a significance level of P<0.05. Duncan’s test showed that treatment P3 (7% chicken intestine) produced the best result and was significantly different from the other treatments. Treatment P3 resulted in the highest absolute weight gain of 23.68 g with a growth rate of 0.79 g/day and an absolute length gain of 2.1 cm with a growth rate of 0.07 cm/day. The FCR values of P1, P2, P3, and P4 were 0,99, 1.38, 1.83, and 1.23, respectively. The survival rate ranged from 96.67% to 100%, while the best feed cost efficiency was obtained in P1 with a value of (Rp 25.05 /g).