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ANTIOXIDANT ACTIVITY OF JUICE OF LOCAL CULTIVAR SWEET POTATOES AS FREE RADICAL SCAVENGERS OF 1,1-DIPHENYL-2-PICRYLHYDRAZYL (DPPH) Palupi, Endah Sri; Mulyati-Sarto, Mulyati-Sarto; Pratiwi, Rarastoeti
Sains & Matematika Vol 1, No 1 (2012): Oktober, Sains & Matematika
Publisher : Sains & Matematika

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Abstract

Sweet potatoes (Ipomoea batatas) are tuber that have high antioxidant activity. The determination of antioxidant activity based on as percentage of inhibition of free radical DPPH. The purpose of this research was to observe the activity of three local cultivars of sweet potatoes by using DPPH method and by using vitamine C as compparison. The measurement of antioxidant activity which performed on sweet potatoes juice and vitamine C with concentration 625 ppm, 1250 ppm, 2500 ppm, 5000 ppm and 10000 ppm by using free radical of DPPH 0,004% that was soluble in ethanol pa and then was measure by using spectrofotometer. Data were analyzed by using ANOVA and LSD (? = 0.05). The result of this research showed that the three cultivar of sweet potatoes juice have antioxidant activity and the purple sweet potatoes cultivar Biru Mangsi had the highest antioxidant activity, although lower than vitamine C. The highest of antioxidant activity were vitamine C, followed by purple sweet potatoe, yellow sweet potatoes (cultivar Kuning Madu) and white sweet potatoes (cultivar Kapasan). 
TAHAPAN PERKEMBANGAN ORGAN REPRODUKSI SEKSUAL PLANARIA DARI PERAIRAN LERENG GUNUNG SLAMET, BATURRADEN, BANYUMAS Palupi, Endah Sri; Puspita Sari, IG.A.Ayu Ratna; Wibowo, Eko Setio
Sains & Matematika Vol 3, No 2 (2015): April, Sains & Matematika
Publisher : Sains & Matematika

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Abstract

Planaria termasuk spesies dalam Filum Platyhelminthes Kelas Turbellaria. Planaria merupakan hewan triploblastik aselomata dengan bentuk tubuh simetri bilateral dengan habitat perairan tawar jernih, perairan laut dan terestrial. Planaria berkembang biak secara aseksual dengan fragmentasi dan secara seksual dengan pembentukan gamet. Penelitian ini bertujuan untuk mendeskripsikan tahapan perkembangan organ reproduksi seksual Planaria dari perairan lereng Gunung Slamet, Baturraden, Banyumas. Planaria diperoleh dengan menangkap menggunakan umpan hati ayam segar, lokasi pengambilan sampel planaria di perairan lereng Gunung Slamet, yaitu Curug Bayan, Lokawisata Baturraden dan Telaga Sunyi Baturraden, Banyumas. Sampel difi ksasi menggunakan Bouin, dibuat sayatan histologis dan diwarnai menggunakan pewarna Hematoxylin dan Eosin untuk mendapatkan struktur mikroanatomi guna identifi kasi tahapan perkembangan organ reproduksi seksualnya. Hasil yang diperoleh dianalisis menggunakan analisis deskriptif. Planaria yang diperoleh di perairan Curug Bayan didominasi ukuran kecil dan sedang dengan perkembangan ovary tahap 2 dan testis tahap 3 dan beberapa tahap 5. Planaria yang diperoleh di perairan Lokawisata Baturraden didominasi ukuran kecil dan sedang dengan perkembangan ovary tahap 2 dan testis tahap 1, sedangkan Planaria yang diperoleh di perairan telaga Sunyi didominasi ukuran sedang dan besar dengan perkembangan ovary tahap 4 dan testis tahap 4. Berdasarkan hasil tersebut dapat disimpulkan bahwa tahapan perkembangan organ reproduksi seksual planaria yang paling matang adalah planaria dari perairan Telaga sunyi yang didominasi planaria berukuran sedang dan besar. Kondisi perairan yang lebih alami dengan temperatur yang lebih rendah dan serasah yang melimpah pada perairan Telaga Sunyi memungkinkan habitat yang sesuai bagi planaria untuk survive dan berkembang biak. Planarian is species of Phylum Platyhelminthes, Class Turbellaria. Planarian is acelomate triplobastic animal, with a body of bilateral simetry and lives in clear freshwater, marine, and terrestrial as its habitat. Planarian reproduces asexually with fragmentation and sexually with gamet formation. The aim of this study was to describe planarian?s sexual reproduction organ development level in mount Slamet slope?s water, Baturraden, Banyumas. Planarians were obtained by baiting with fresh chicken liver; location of sampling on Mount Slamet were Curug Bayan, Lokawisata Baturraden, and Telaga Sunyi, Baturraden, Banyumas. Samples were fi xated with Bouin and stained with Hematoxylin and Eosin for histological structure to identify the sexual reproduction organ development stage. The results were analyzed descriptively. Total number of obtained planarian varied, however it wasn?t signifi cantly diferrent among sampling locations. Planarians of Curug Bayan were mostly in small to moderate size with ovary development of stage 2, testis of stage 3 and some of stage 5. Planarians of Lokawisata Baturraden were mostly in small to moderate size with ovary development of stage 2 and testis of stage 1. Planarians of Telaga Sunyi were mostly in moderate and big size with ovary development of stage 4 and testis of stage 4. It was concluded that planarian of Telaga Sunyi, with mostly moderate and big size, had the most matured sexual reproduction organs development. More natural water condition including lower temperature and abundant litter in Telaga Sunyi allows suitable habitat for planarian survival and planarian breeding.
Tahapan Perkembangan Organ Reproduksi Seksual Planaria dari Perairan Lereng Gunung Slamet, Baturraden, Banyumas Palupi, Endah Sri; Puspita Sari, IG.A.Ayu Ratna; Wibowo, Eko Setio
Sains & Matematika Vol 3, No 2 (2015): April, Sains & Matematika
Publisher : Sains & Matematika

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Abstract

Planaria termasuk spesies dalam Filum Platyhelminthes Kelas Turbellaria. Planaria merupakan hewan triploblastik aselomata dengan bentuk tubuh simetri bilateral dengan habitat perairan tawar jernih, perairan laut dan terestrial. Planaria berkembang biak secara aseksual dengan fragmentasi dan secara seksual dengan pembentukan gamet. Penelitian ini bertujuan untuk mendeskripsikan tahapan perkembangan organ reproduksi seksual Planaria dari perairan lereng Gunung Slamet, Baturraden, Banyumas. Planaria diperoleh dengan menangkap menggunakan umpan hati ayam segar, lokasi pengambilan sampel planaria di perairan lereng Gunung Slamet, yaitu Curug Bayan, Lokawisata Baturraden dan Telaga Sunyi Baturraden, Banyumas. Sampel difi ksasi menggunakan Bouin, dibuat sayatan histologis dan diwarnai menggunakan pewarna Hematoxylin dan Eosin untuk mendapatkan struktur mikroanatomi guna identifi kasi tahapan perkembangan organ reproduksi seksualnya. Hasil yang diperoleh dianalisis menggunakan analisis deskriptif. Planaria yang diperoleh di perairan Curug Bayan didominasi ukuran kecil dan sedang dengan perkembangan ovary tahap 2 dan testis tahap 3 dan beberapa tahap 5. Planaria yang diperoleh di perairan Lokawisata Baturraden didominasi ukuran kecil dan sedang dengan perkembangan ovary tahap 2 dan testis tahap 1, sedangkan Planaria yang diperoleh di perairan telaga Sunyi didominasi ukuran sedang dan besar dengan perkembangan ovary tahap 4 dan testis tahap 4. Berdasarkan hasil tersebut dapat disimpulkan bahwa tahapan perkembangan organ reproduksi seksual planaria yang paling matang adalah planaria dari perairan Telaga sunyi yang didominasi planaria berukuran sedang dan besar. Kondisi perairan yang lebih alami dengan temperatur yang lebih rendah dan serasah yang melimpah pada perairan Telaga Sunyi memungkinkan habitat yang sesuai bagi planaria untuk survive dan berkembang biak. Planarian is species of Phylum Platyhelminthes, Class Turbellaria. Planarian is acelomate triplobastic animal, with a body of bilateral simetry and lives in clear freshwater, marine, and terrestrial as its habitat. Planarian reproduces asexually with fragmentation and sexually with gamet formation. The aim of this study was to describe planarian’s sexual reproduction organ development level in mount Slamet slope’s water, Baturraden, Banyumas. Planarians were obtained by baiting with fresh chicken liver; location of sampling on Mount Slamet were Curug Bayan, Lokawisata Baturraden, and Telaga Sunyi, Baturraden, Banyumas. Samples were fi xated with Bouin and stained with Hematoxylin and Eosin for histological structure to identify the sexual reproduction organ development stage. The results were analyzed descriptively. Total number of obtained planarian varied, however it wasn’t signifi cantly diferrent among sampling locations. Planarians of Curug Bayan were mostly in small to moderate size with ovary development of stage 2, testis of stage 3 and some of stage 5. Planarians of Lokawisata Baturraden were mostly in small to moderate size with ovary development of stage 2 and testis of stage 1. Planarians of Telaga Sunyi were mostly in moderate and big size with ovary development of stage 4 and testis of stage 4. It was concluded that planarian of Telaga Sunyi, with mostly moderate and big size, had the most matured sexual reproduction organs development. More natural water condition including lower temperature and abundant litter in Telaga Sunyi allows suitable habitat for planarian survival and planarian breeding.
Natural Antioxidants Effect on the Performance of Erythrocytes, Leucocytes, and Hb Levels of Pregnant Mice Exposed to the Plastic Palupi, Endah Sri; Wibowo, Eko Setio; Puspitasari, IGA. Ayu Ratna; Atang
Biota Vol 11 No 1 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/

Abstract

Plastic is a widely used material with several toxic components, such as free radicals. HDPE plastic is considered safer but requires more attention due to increased usage. Exogen antioxidant is needed to neutralize free radicals. This research aimed to evaluate the effect of natural antioxidants on the performance of erythrocytes, leucocytes, and hemoglobin levels of pregnant mice exposed to plastic compounds. The study used a completely randomized design with six groups and four replicates each. Those groups were aquadest control, plastic chemical compounds (PCC) control, PCC + carrot juice, PCC + orange juice, PCC + purple sweet potato juice, and PCC + mung bean sprouts juice. The PCC was obtained from boiling HDPE plastic, while natural antioxidant juice was from pure sources. Pregnant mice were treated with 0.25 ml of each orally for 21 days, starting from the first gestational day. Blood was collected from a caudal vein. Erythrocytes and leucocytes were counted using a hemocytometer, and Hb levels were measured by the Sahli method. Blood cell morphology was assessed using Giemsa-stained blood smears. Statistical data were analyzed by one-way ANOVA using GraphPad Prism ver.10.0 software, while morphology data were analyzed descriptively. The results showed that PCC and natural antioxidants did not impact the number of erythrocytes, leucocytes, cell morphology, and Hb levels of pregnant mice. However, natural antioxidants may stabilize the erythrocyte total and Hb levels after the treatments are completed.