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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Natural Antioxidants Effect on the Performance of Erythrocytes, Leucocytes, and Hb Levels of Pregnant Mice Exposed to the Plastic Palupi, Endah Sri; Wibowo, Eko Setio; Puspitasari, IGA. Ayu Ratna; Atang
Biota Vol 11 No 1 (2025): Jurna Biota 2025
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/

Abstract

Plastic is a widely used material with several toxic components, such as free radicals. HDPE plastic is considered safer but requires more attention due to increased usage. Exogen antioxidant is needed to neutralize free radicals. This research aimed to evaluate the effect of natural antioxidants on the performance of erythrocytes, leucocytes, and hemoglobin levels of pregnant mice exposed to plastic compounds. The study used a completely randomized design with six groups and four replicates each. Those groups were aquadest control, plastic chemical compounds (PCC) control, PCC + carrot juice, PCC + orange juice, PCC + purple sweet potato juice, and PCC + mung bean sprouts juice. The PCC was obtained from boiling HDPE plastic, while natural antioxidant juice was from pure sources. Pregnant mice were treated with 0.25 ml of each orally for 21 days, starting from the first gestational day. Blood was collected from a caudal vein. Erythrocytes and leucocytes were counted using a hemocytometer, and Hb levels were measured by the Sahli method. Blood cell morphology was assessed using Giemsa-stained blood smears. Statistical data were analyzed by one-way ANOVA using GraphPad Prism ver.10.0 software, while morphology data were analyzed descriptively. The results showed that PCC and natural antioxidants did not impact the number of erythrocytes, leucocytes, cell morphology, and Hb levels of pregnant mice. However, natural antioxidants may stabilize the erythrocyte total and Hb levels after the treatments are completed.
Characteristics of Water Storage Capacity and Water Storage Efficiency of ‘Biotextile’ Growing Medium for Erosion Resistance Utami, Dyah Nursita; Kusumastuti, Etty; Sudiana, Nana; Rahayu, Budi; Hidayat, Nur; Sulistiawan, Insan Nur; Purnomo, Agus; Atang; Sittadewi, Euthalia Hanggari
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.5891

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Abstract: Biotextile is an innovation in erosion-resistant growing media technology, made from organic cocopeat supplemented with Legume Cover Crop (LCC) seeds and coated with biofertilizer. In its application, the success of biotextiles is determined, among other things, by the capacity and efficiency of water storage in the media The capacity and water storage efficiency of biotextile media will determine the success of growth in field applications. From field tests conducted on the sloping land of the Ciater reservoir cliffs, South Tangerang, it was shown that on the 4th day the LCC seeds had started to grow, on the 10th day they had grown in all biotextile cells, and at the age of 3 months the LCC plant canopy had covered almost all of the soil surface at the test field. Based on the growth test results, biotextile characterization tests have been carried out, which include measuring water content (KA), water storage capacity (KPA), and water storage efficiency (Es). Based on these test results, a biotextile characteristic test has been carried out which includes water content (KA), water storage capacity (KPA), and water storage efficiency (Es) to find out the characteristics of KPA and Es in evaluating its success and to prove the hypothesis that KPA and biotextile Es support the successful growth of land cover plants (LCC). The measurement of KA biotextile was carried out using the gravimetric method, while KPA and Es were measured using the immersion method, which was carried out in the Serpong laboratory. From the results of laboratory analysis, KA reached a range of values: 40–80%, KPA = 74.41–297.59%, and Es = 21%–75.5%. Apart from the results of plant growth and laboratory results, from a physical perspective, the performance of biotextiles can be seen in reducing the energy of rainwater blows, reducing the flow rate of surface water, and reducing soil erosion.
Molecular Identification of Color Variants of Procambarus clarkii Using the COI Gene for Taxonomic Validation Bhagawati, Dian; Husein Sastranegara, Muhammad; Agus Nuryanto; Anastasia Endang Pulungsari; Elly Tuti Winarni; Atang; Hanan Hassan Alsheikh Mahmoud
Advance Sustainable Science Engineering and Technology Vol. 7 No. 2 (2025): February-April
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/hcaz9264

Abstract

This study addresses taxonomic uncertainty surrounding color variants of Procambarus clarkii in aquaculture and conservation contexts. We investigated whether commercially significant color morphs represent distinct subspecies or phenotypic variations of a single species. Using the cytochrome c oxidase subunit I (COI) gene as a molecular marker, we analyzed four color morphs (blue, white, orange, and brown) from aquaculture facilities in Banyumas Regency. DNA extraction, PCR amplification, and sequencing were performed on 20 specimens. Results showed high sequence homology (98.7-99.8%) across all variants, confirming they belong to a single species. Genetic distance analysis revealed minimal divergence (0.2-1.3%), insufficient for subspecies classification. Phylogenetic reconstruction demonstrated specimens clustered by genetic similarity rather than color or geographic origin, indicating coloration results from genetic mutations rather than environmental adaptations. This COI-based approach provides a molecular framework for taxonomic classification of P. clarkii varieties, with implications for breeding programs, variety certification, and management of this economically important yet potentially invasive species.
Co-Authors Agus Nuryanto Agus Purnomo Budi Rahayu, Budi Eko Setio Wibowo Elly Tuti Winarni Euthalia Hanggari Sittadewi Hanan Hassan Alsheikh Mahmoud Husein Sastranegara, Muhammad Kusumastuti, Etty Nana Sudiana Nur Hidayat Palupi, Endah Sri Pulungsari, Anastasia Endang Puspitasari, IGA. Ayu Ratna Sulistiawan, Insan Nur Utami, Dyah Nursita