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All Journal Jurnal Ilmu dan Teknologi Kelautan Tropis Prosiding Seminar Biologi Biogenesis: Jurnal Ilmiah Biologi Indonesian Journal of Cancer Chemoprevention Prosiding Pendidikan Sains Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA Journal of Health (JoH) Indonesian Aquaculture Journal Jurnal Biomedika Sains dan Matematika Proceeding Biology Education Conference Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Warta LPM Jurnal Ilmiah Ilmu Kesehatan
Rarastoeti Pratiwi
Department Of Biology, Faculty Of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Cloning and Expression of ORF124 Koi Herpesvirus as a Vaccine ., Murwantoko; Pratiwi, Rarastoeti; Kawaichi, Masashi
Indonesian Journal of Biotechnology Vol 17, No 1 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Koi herpesvirus (KHV) which also known as Cyprinid herpesvirus 3 (CyHV-3), Koi herpes-like virus, and carp interstitial nephritis gill necrosis virus (CNGV), caused signifi cant morbidity and mortality in koi and common carp (Cyprinus carpio). The case fatality rate of this disease is 80–100%. Glycoprotein has been used for vaccine development as sub unit vaccine against viruses. The aim of this research was to clone and express membrane glycoprotein ORF124 KHV as a candidate of recombinant vaccine. ORF124 KHV gene was successfully cloned into pBSKS and sequenced. Result showed that ORF124 KHV (isolate from Indonesia) had 100 % similarity with Cyprinid herpesvirus 3 strain TUMST1 (from Japan), 99% similarity with Koi herpesvirus strain KHV-U (from USA) and Koi herpesvirus strain KHV-I (from Israel). Prediction analysis of T and B cell epitopes showed that ORF124 KHV protein had 14 and 11 T cell epitopes (IAd, Rothbard/Taylor pattern),and had 10 B cell epitopes, suggested that the protein can be used as a vaccine candidate. ORF124 gene has been expressed in Escherichia coli under pET32-a(+)vector.
Cloning and Sequence Analysis of Capsid Protein Gene of Iridovirus Indonesian Isolates ., Murwantoko; Handayani, Christina Retna; Pratiwi, Rarastoeti
Indonesian Journal of Biotechnology Vol 14, No 1 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Iridovirus was known as agents that caused serious systemic disease in freshwater and marine fishes. The mortality up to 100% of orange-spotted grouper (Epinephelus coioides) due to iridovirus infection has been reported in Indonesia. The gene encoding capsid protein of iridovirus is supposed to be conserved and has the potency for the development of control methods. The objectives of this study are to clone the gene encoding capsid protein iridovirus and to analyze their sequences. The   spleen tissues of orange-spotted grouper were collected and extracted their DNA. The DNA fragment of capsid protein of iridovirus genes were amplified by PCR using designed primers with the extraction DNA as templates. The amplified DNA fragments were cloned in pBSKSII and sequenced.  The genes encoding capsid protein of iridovirus from Jepara and Bali were successfully amplified and cloned. The Jepara clone (IJP03) contained complete open reading frame (ORF) of the gene composed by 1362 bp nucleotides which encoded 453 amino acids. Those Jepara and Bali (IGD01) clones shared 99.8% similarity in nucleotide level and 99.4% at amino acid level. Based on those sequences, Indonesian iridovirus was belonged to genus Megalocystivirus and shared 99,6-99,9% similarity on nucleotide level with DGIV, ISKNV, MCIV, and ALIV
Characterization of envelope-transmembrane Gene of Jembrana Disease Virus Tabanan 1995 Isolate Kusumawati, Asmarani; Pratiwi, Rarastoeti; Astuti, Pudji; Hamid, Penny Humaidah
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

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Abstract

The availability of specific and rapid detection methods is essential for monitoring the health status of farmed species, particularly in viral disease as in this case early diagnosis is a critical factor in containing disease outbreaks. Jembrana Disease Virus (JDV) is a lentivirus that causes an acute, severe disease syndrome in infected Bali cattle in Indonesia, resulting in heavy economic losses because of the high mortalities. The virus-host interaction and the modes of transmission are still unknown. The goal of the research was to designa probe candidate of Jembrana Disease Virus based on envelope-transmembrane (env-tm) gene to optimize Jembrana disease detection method. The DNA fragment derived from env-tm of JDV was used, cloned in pGEX-TM and expressed in E.coli DH 5α. Sequence analysis was conducted with BLAST programs from NCBI. Sequence analyses of the fragments of env-tm clone, indicated that it has a very closed genetic relation with 97,68% homology identity. Probe was designed based on the conserved region of env-tm using Geneious resulted in JT2 252 bp long. BLAST analyses showed that probes had high specifity to other strains of JDV in Indonesia.Key words : probe, env-tm, JDV, specifity, sensitivity.
DETEKSI GAMMA AMINO BUTYRIC ACID (GABA) PADA DAUN Artocarpus altilis Indrowati, Meti; Astuti, Pudji; Pratiwi, Rarastoeti; Rumiyati, Rumiyati
Prosiding SNPS (Seminar Nasional Pendidikan Sains) 2015: Prosiding SNPS (Seminar Nasional Pendidikan Sains)
Publisher : Universitas Sebelas Maret Surakarta

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Abstract

Senyawa aktif Gamma Amino Butyric Acid (GABA) merupakan asam amino yang terdapat di otak mammalia dan berperan sebagai neurotransmiter.  Selain pada sistem saraf pusat, GABA juga terdapat dalam konsentrasi tinggi di sel β pankreas serta memiliki potensi sebagai antidiabetes. GABA dapat disintesis oleh hewan, bakteri asam laktat dan tumbuhan.  Penelitian dilakukan dengan tujuan mendeteksi keberadaan GABA dalam bagian tumbuhan yaitu daun Artocarpus altilis.  Deteksi dilakukan dengan Kromatografi Lapis Tipis (KLT) menggunakan fase diam plat silika, fase gerak BAW(3:1:1), dan GABA murni sebagai pembanding.  Larutan GABA dibuat dengan pelarut etanol:air (7:3).  Hasil penelitian menunjukkan GABA terdeteksi pada ekstrak daun Artocarpus altilis.
Effect of Tokay Gecko (Gekko gecko LINNAEUS, 1758) Saliva on Angiogenesis During Wound Healing Phase of Autotomized Tail in Common Sun Skink (Eutropis multifasciata KUHL, 1820) Inayah, Nurul; Soesilo, Nyoman Puniawati; Pratiwi, Rarastoeti
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1344.355 KB) | DOI: 10.14203/jbi.v13i2.3399

Abstract

ABSTRACTThe purpose of this study was to investigate the effect of Tokay gecko saliva on morphology and angiogenesis response on the healing process of skink tail wound and also to characterize the protein profile of Gecko saliva. Twelve skinks were autotomized and wound surface of tail smeared by young gecko saliva, adult gecko saliva, and human’s saliva twice per day and control. The morphological changes of the wound surface were observed. The angiogenesis response was observed in vitro using Chorioallantois Membrane (CAM) of the ninth days chick embryos. Protein profile of gecko saliva analyzed with SDS-PAGE. Generally, treated wound showed a better healing. Young gecko saliva able to stimulate angiogenesis in wound healing stage of sun skink tail after autotomy. Saliva protein of young and adult Gecko differences was not only in the size (or density) but also in the number of the bands. The young and adult Gecko revealed a striking consistency of protein patterns, indicating a profound physiological stability of the whole saliva. This study showed that Gecko saliva able to accelerate wound healing, thus Gecko saliva may have potential me as a future therapeutic for treating infected wounds.Keywords: Tokay gecko saliva, wound healing, sun skink tail, angiogenesis, protein profile.
Pengaruh Perbedaan Waktu Awal Pemberian Pakan Buatan terhadap Pertumbuhan dan Aktivitas Enzim Pencernaan Larva Ikan Kerapu Bebek Cromileptes altivelis (Valenciennes 1828) Melianawati, Regina; Pratiwi, Rarastoeti; Astuti, Ni Wayan Widya
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3055

Abstract

The effect of different initial time of artificial feeding to the growth and the activities of digestive enzymes of humpback grouper larvae Cromileptes altivelis (Valenciennes 1828).Humpback grouper is an economically marine finfish commodity, so it is important to scale up its culture. In aquaculture, feed is dominantly affecting to fish growth. Besides live feeds, theartificial feed is also given to fulfill the need of nutrient for larvae. However, the capability of larvae to digest the artificial feed is still limited because of its simplicity and immaturity of digestive system structure. Digestive enzymes are a biological indicator to figure out the capability of larvae to digest the artificial feed. This research was conducted to find out the influence of different initial time of artificial feeding to the growth and digestive enzyme activity of humpback grouper larvae. Larvae rearing were done in hatchery. Larvae were fed with live feed rotifers started in the evening of the second day. The treatment given in this research was the difference initial time of artificial feeding, i.e: (A) given at 8 days old larvae and (B) given at13 days old larvae. The variable observations were the growth and digestive enzyme activity of larvae. The results indicate that the different initial time of artificial feeding influenced to thegrowth and digestive enzyme activity of humpback grouper larvae. Total length, body weight, length of dorsal spine and ventral spine of 30 days old humpback grouper larvae on treatment A were 17.47±2.35 mm, 0.11±0.04 g, 6.83±0.45 mm and 5.07±0.50 mm, respectively, while on treatment B were 13.23±2.53 mm, 0.04±0.02 g, 5.73±1.11 mm and 4.48±0.50 mm, respectively. Up to 30 days old larvae, protease and amylase enzymes activities of larvae on treatment A was higher than on treatment B, while lipase enzymes activity of larvae on treatment B was higher than on treatment A. Therefore, the different initial time of artificial feeding gives the different effect on the growth and the activities of protease, amylase and lipase enzymes of humpback grouper larvae.Key words: artificial feed, growth, digestive enzyme activity, humpback grouper, larvae
MUTASI MISSENSE (P.374PHE/LEU) PADA EKSON 5 GEN MATP, PENYEBAB OCULOCUTANEOUS ALBINISM TIPE 4 (OCA4) DI WONOSOBO, JAWA TENGAH Nur Handayani, Niken Satuti; Sukmawati, Feri; Pratiwi, Rarastoeti
Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK Albinisme merupakan kelainan genetik autosomal resesif  berupa gangguan sintesis melanin yang terjadi pada manusia. Albinisme dibagi menjadi dua kelompok besar, yaitu Ocular Albinism (OA) dan Oculocutaneous Albinism (OCA). Berdasarkan gen yang mengalami mutasi, OCA dibedakan menjadi 4 tipe yaitu OCA1, OCA2, OCA3 dan OCA4. OCA4 disebabkan mutasi pada gen MATP. Penelitian yang telah dilakukan dengan PCR-SSCP (Polymerase Chain Reaction-Single Stranded Conformation Polymorphism) mendeteksi adanya mutasi pada ekson 5 gen MATP, pada penderita albinisme di Wonosobo, Jawa Tengah. Sekuensing ekson 5 gen MATP dilakukan untuk mengidentifikasi tipe mutasinya. DNA diisolasi dari sampel darah penderita dan digunakan sebagai template untuk amplifikasi ekson 5 gen MATP dengan metode PCR. Produk PCR selanjutnya digunakan sebagai template untuk sekuensing dengan metode Sanger. Hasil sekuensing dianalisis menggunakan program Clustal-W dan dibandingkan dengan sekuens ekson 5 gen MATP dari International DNA Data Base (nomer akses AF172849.1). Hasil analisis menunjukkan adanya pada perubahan basa nukleotida no.1122 dari C menjadi G (c.1122 C>G) yang mengakibatkan mutasi missense, yaitu fenilalanin menjadi leusin, pada asam amino nomer 374 (p.374 Phe/Leu).   Kata kunci : OCA4, mutasi, ekson 5, gen MATP
EFFECT OF TOKAY GECKO (GEKKO GECKO LINNAEUS, 1758) SALIVA ON ANGIOGENESIS DURING WOUND HEALING PHASE OF AUTOTOMIZED TAIL IN COMMON SUN SKINK (EUTROPIS MULTIFASCIATA KUHL, 1820) Inayah, Nurul; Soesilo, Nyoman Puniawati; Pratiwi, Rarastoeti
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3399

Abstract

ABSTRACTThe purpose of this study was to investigate the effect of Tokay gecko saliva on morphology and angiogenesis response on the healing process of skink tail wound and also to characterize the protein profile of Gecko saliva. Twelve skinks were autotomized and wound surface of tail smeared by young gecko saliva, adult gecko saliva, and human?s saliva twice per day and control. The morphological changes of the wound surface were observed. The angiogenesis response was observed in vitro using Chorioallantois Membrane (CAM) of the ninth day's chick embryos. Protein profile of gecko saliva analyzed with SDS-PAGE. Generally, treated wound showed a better healing. Young gecko saliva able to stimulate angiogenesis in wound healing stage of sun skink tail after autotomy. Saliva protein of young and adult Gecko differences was not only in the size (or density) but also in the number of the bands. The young and adult Gecko revealed a striking consistency of protein patterns, indicating a profound physiological stability of the whole saliva. This study showed that Gecko saliva able to accelerate wound healing, thus Gecko saliva may have potential me as a future therapeutic for treating infected wounds.Keywords: Tokay gecko saliva, wound healing, sun skink tail, angiogenesis, protein profile.
PENGARUH PERBEDAAN WAKTU AWAL PEMBERIAN PAKAN BUATAN TERHADAP PERTUMBUHAN DAN AKTIVITAS ENZIM PENCERNAAN LARVA IKAN KERAPU BEBEK CROMILEPTES ALTIVELIS (VALENCIENNES 1828) Melianawati, Regina; Pratiwi, Rarastoeti; Astuti, Ni Wayan Widya
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3055

Abstract

The effect of different initial time of artificial feeding to the growth and the activities of digestive enzymes of humpback grouper larvae Cromileptes altivelis (Valenciennes 1828).Humpback grouper is an economically marine finfish commodity, so it is important to scale up its culture. In aquaculture, feed is dominantly affecting to fish growth. Besides live feeds, theartificial feed is also given to fulfill the need of nutrient for larvae. However, the capability of larvae to digest the artificial feed is still limited because of its simplicity and immaturity of digestive system structure. Digestive enzymes are a biological indicator to figure out the capability of larvae to digest the artificial feed. This research was conducted to find out the influence of different initial time of artificial feeding to the growth and digestive enzyme activity of humpback grouper larvae. Larvae rearing were done in hatchery. Larvae were fed with live feed rotifers started in the evening of the second day. The treatment given in this research was the difference initial time of artificial feeding, i.e: (A) given at 8 days old larvae and (B) given at13 days old larvae. The variable observations were the growth and digestive enzyme activity of larvae. The results indicate that the different initial time of artificial feeding influenced to thegrowth and digestive enzyme activity of humpback grouper larvae. Total length, body weight, length of dorsal spine and ventral spine of 30 days old humpback grouper larvae on treatment A were 17.47±2.35 mm, 0.11±0.04 g, 6.83±0.45 mm and 5.07±0.50 mm, respectively, while on treatment B were 13.23±2.53 mm, 0.04±0.02 g, 5.73±1.11 mm and 4.48±0.50 mm, respectively. Up to 30 days old larvae, protease and amylase enzymes activities of larvae on treatment A was higher than on treatment B, while lipase enzymes activity of larvae on treatment B was higher than on treatment A. Therefore, the different initial time of artificial feeding gives the different effect on the growth and the activities of protease, amylase and lipase enzymes of humpback grouper larvae.Key words: artificial feed, growth, digestive enzyme activity, humpback grouper, larvae
ANTIOXIDANT ACTIVITY OF JUICE OF LOCAL CULTIVAR SWEET POTATOES AS FREE RADICAL SCAVENGERS OF 1,1-DIPHENYL-2-PICRYLHYDRAZYL (DPPH) Palupi, Endah Sri; Mulyati-Sarto, Mulyati-Sarto; Pratiwi, Rarastoeti
Sains & Matematika Vol 1, No 1 (2012): Oktober, Sains & Matematika
Publisher : Sains & Matematika

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Abstract

Sweet potatoes (Ipomoea batatas) are tuber that have high antioxidant activity. The determination of antioxidant activity based on as percentage of inhibition of free radical DPPH. The purpose of this research was to observe the activity of three local cultivars of sweet potatoes by using DPPH method and by using vitamine C as compparison. The measurement of antioxidant activity which performed on sweet potatoes juice and vitamine C with concentration 625 ppm, 1250 ppm, 2500 ppm, 5000 ppm and 10000 ppm by using free radical of DPPH 0,004% that was soluble in ethanol pa and then was measure by using spectrofotometer. Data were analyzed by using ANOVA and LSD (? = 0.05). The result of this research showed that the three cultivar of sweet potatoes juice have antioxidant activity and the purple sweet potatoes cultivar Biru Mangsi had the highest antioxidant activity, although lower than vitamine C. The highest of antioxidant activity were vitamine C, followed by purple sweet potatoe, yellow sweet potatoes (cultivar Kuning Madu) and white sweet potatoes (cultivar Kapasan). 
Co-Authors A.A. Ketut Agung Cahyawan W Adania, Baik Aisyah Afifah, Fitri Nur ALIM ISNANSETYO Almunawar, Muhammad Fikri Ardaning Nuriliani Asmarani Kusumawati Asmarani Kusumawati Cahyadi, G.A.B Yehezkiel P. Christina Retna Handayani Christina Retna Handayani, Christina Retna Dewi Nur'aeni Setyowati Endah Sri Palupi Fahhlevi, Nabilah Adzra Fajar Sofyantoro Fathurrohmah, Siti Feri Sukmawati Firman Alamsyah Hartati, Yuni Hidayat, Rheina Faticha Asyamsa L. Hartanto Nugroho Lanjar Lisna Hidayati, Lisna Maharesi, Chesa Ekani Masashi Kawaichi Masashi Kawaichi, Masashi Mataram, Made Bagus Auriva Maturbongs, Agatha Maturbongs, Rudi A. Maulina, Nurina Tahta Afwi Meti Indrowati Mulyati-Sarto Mulyati-Sarto Mulyati-Sarto, Mulyati-Sarto Murwantoko . Nasution, Nurlita Putri Bela Ni Wayan Widya Astuti Niken Satuti Nur Handayani Nurhidayah, Septika Nurul Inayah Nyoman Puniawati Soesilo Palupi, Endah Sri Paramita Sari, Indah Penny Humaidah Hamid Penny Humaidah Hamid, Penny Humaidah PUDJI ASTUTI Purnami, S.E. raudatul jannah Regina Melianawati Rina Puji Astuti Rizal Maarif Rukmana Rumiyati, Rumiyati Sarbini Soesilo, Nyoman Puniawati Soesilo, Nyoman Puniawati SUWARNO HADISUSANTO Syam, Adi Mazdi Triana Nuryastuti Trijoko Trijoko Trijoko Trijoko Yekti Asih Purwestri