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Gusmawartati, .
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Isolation of Cellulolytic Bacteria from Peat Soils as Decomposer of Oil Palm Empty Fruit Bunch Gusmawartati, .; Agustian, .; Herviyanti, .; Jamsari, .
JOURNAL OF TROPICAL SOILS Vol. 22 No. 1: January 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i1.47-53

Abstract

The aim of the research was to find out potential strainsof cellulolytic bacteria isolated from two tropical peat soils and to studythe potency of the isolated bacteria to decompose oil palm empty fruit bunch (EFB). The research was carried out in two stages: (1) isolation of cellulolytic bacteria from peat soils and (2) testing the potency of isolated bacteria to decompose oil palm EFB. The cellulolytic bacteria were isolated from two peat soils, i.e. a natural peat soil (forest) and a cultivated peat soil (has been used as agriculture land). Isolation of cellulolytic bacteria was conducted by preparing a series dilution of culture solutions using a streak plate method in a carboxymethyl cellulose(CMC) selective medium.Isolates that were able to form clear zones surrounding their bacterial colony were further tested to study the potency of the isolates to decompose cellulose in oil palm EFB. The cellulolytic activity of the selected isolates were further determined via production of reducing sugars in an oil palm EFB liquid medium using Nelson-Somogyi method. The results showed that there are six isolates of cellulolytic bacteria that have been identified in two tropical peat soils used in the current study. Two isolates are identified in a natural peat soil (forest) and four isolates are identified in a cultivated peat soil. The isolates collected are identified as Bacillus sp., Pseudomonassp. and Staphylococcus sp. Among the isolates, an isolate of GS II-1 produces the highest concentration of reducing sugars, namely 0.1012 unitmL-1or 101 ppm, indicating that the isolate of GS II-1 is highly potential to decompose oil palm EFB. Therefore, the isolate of GS II-1 can be used as a decomposer in the bio-conversion processes of oil palm EFB.Keywords: isolation, bacteria, cellulolytic, oil palm empty fruit bunch (EFB), peat soil
Effect Various Combination of Organic Waste on Compost Quality Hapsoh, .; Gusmawartati, .; Yusuf, Muhammad
JOURNAL OF TROPICAL SOILS Vol. 20 No. 1: January 2015
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2015.v20i1.59-65

Abstract

Municipal solid waste and agricultural waste have different ratio C/N and nutrients contents. They can be used as compost row materials. The purpose of the research was to get an optimum combination of both wastes to improve compost quality, to meet the Indonesian National Standard 19-7030-2004. Composting process use pots. The treatments were twelve combination of municipal solid waste (garbage market, household waste, restaurant waste) and agricultural waste (rice straw, empty fruit bunches of oil palm, cassava peel, banana skin) with a ratio of 1:1 and enriche by chicken manure, cow manure, wood ash and cellulolytic microorganisme. The treatment were replicated three times. The results showd that the nutrients content of compost were 0.77 to 1.19% nitrogen, 0.23 to 0.30% phosphorus, 0.46 to 0.69% potassium and 15.48 to 34.69% organic matter. The combination of agricultural waste and municipal solid waste affected the quality of compost. Compost that meets SNI 19-7030-2004 is a combination of rice straw+market waste that contains 1.12% nitrogen, 0.28% phosphorus, 0.63% potassium, ratio C/N 19.50, pH 7.42, and organic matters 37.65%. [How to Cite: Hapsoh, Gusmawartati, and M Yusuf. 2015. Effect Various Combination of Organic Waste on Compost Quality. J Trop Soils 19: 59-65. Doi: 10.5400/jts.2015.20.1.59][Permalink/DOI: www.dx.doi.org/10.5400/jts.2015.20.1.59]
Co-Authors . Agustian, . . HERVIYANTI . Jamsari Hapsoh Muhammad Yusuf