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Isolation of Cellulolytic Bacteria from Peat Soils as Decomposer of Oil Palm Empty Fruit Bunch Gusmawartati, .; Agustian, .; Herviyanti, .; Jamsari, .
JOURNAL OF TROPICAL SOILS Vol. 22 No. 1: January 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i1.47-53

Abstract

The aim of the research was to find out potential strainsof cellulolytic bacteria isolated from two tropical peat soils and to studythe potency of the isolated bacteria to decompose oil palm empty fruit bunch (EFB). The research was carried out in two stages: (1) isolation of cellulolytic bacteria from peat soils and (2) testing the potency of isolated bacteria to decompose oil palm EFB. The cellulolytic bacteria were isolated from two peat soils, i.e. a natural peat soil (forest) and a cultivated peat soil (has been used as agriculture land). Isolation of cellulolytic bacteria was conducted by preparing a series dilution of culture solutions using a streak plate method in a carboxymethyl cellulose(CMC) selective medium.Isolates that were able to form clear zones surrounding their bacterial colony were further tested to study the potency of the isolates to decompose cellulose in oil palm EFB. The cellulolytic activity of the selected isolates were further determined via production of reducing sugars in an oil palm EFB liquid medium using Nelson-Somogyi method. The results showed that there are six isolates of cellulolytic bacteria that have been identified in two tropical peat soils used in the current study. Two isolates are identified in a natural peat soil (forest) and four isolates are identified in a cultivated peat soil. The isolates collected are identified as Bacillus sp., Pseudomonassp. and Staphylococcus sp. Among the isolates, an isolate of GS II-1 produces the highest concentration of reducing sugars, namely 0.1012 unitmL-1or 101 ppm, indicating that the isolate of GS II-1 is highly potential to decompose oil palm EFB. Therefore, the isolate of GS II-1 can be used as a decomposer in the bio-conversion processes of oil palm EFB.Keywords: isolation, bacteria, cellulolytic, oil palm empty fruit bunch (EFB), peat soil
The Properties of Humic Acids Extracted from Four Sources of Organic Matters and Their Ability to Bind Fe2+ at New Established Rice Field Herviyanti, .; Prasetyo, Teguh Budi; Ahmad, Fachri; Darmawan, .
JOURNAL OF TROPICAL SOILS Vol. 15 No. 3: September 2010
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2010.v15i3.237-244

Abstract

The Properties of Humic Acids Extracted from Four Sources of Organic Matters and Their Ability to Bind Fe2+ at New Established Rice Field (Herviyanti, TB Prasetyo, F Ahmad and Darmawan):  In order to identify the properties of humic acid extracted from four kinds of organic matters (a peat soil, a stable manure, a compost of rice straw and a municipal waste)  and their potentiality to bind Fe2+  at new established rice field, a series of experiment was done in Soil Laboratory Faculty of Agriculture, Andalas University Padang. First step was characterization of functional groups and other chemical properties of humic acids, and their reaction with Fe2+. The second step was to examine the ability of humic acids to bind Fe2+ solution at new established rice field by conducting incubation experiments.  The experiment used  a completely randomized design with three replications.  The 450 ppm Fe solution was treated with 0, 50, 100, 150, 200, 250, 300, 350, 400, and 450 ppm humic acids and incubated for 24 hours.  While top soil samples taken from Sitiung, West Sumatera were treated with 0,100, 200, 300, and 400 ppm humic acids, flooded with deionized water, and incubated for 6 weeks.  The result showed that   the functional group and other chemical characteristic of humic acid from rice straw compost and peat soil were better than those of manure and municipal waste compost. Functional group of both humic acids was dominated by COO-. High reactivity of the humic acid had been found when humic acids were added to Fe solution with ratio 1 : 1. Use of humic acid extracted from peat soil with the levels from 0 to 100, 200, 300, and 400 ppm decreased the Fe2+ concentration from 1.361 ppm to  910, 860, 831, and 776 ppm, respectively at new established rice field.  While the use of humic acid extracted from  rice straw compost with the same levels as above decreased the Fe2+ concentration  from 1361 to 770, 701, 612, and 600 ppm, respectively, after four weeks of flooding.
Humic Acid and Water Management to Decrease Ferro (Fe2+) Solution and Increase Productivity of Established New Rice Field Herviyanti, .; Prasetyo, Teguh Budi; Ahmad, Fachri; Saidi, Amrizal
JOURNAL OF TROPICAL SOILS Vol. 17 No. 1: Januari 2012
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2012.v17i1.9-17

Abstract

The purpose of this research was to gain a technological breakthrough in controlling Fe toxicity (Fe2+) on Ultisol in a new established rice field by using humic acid from rice straw compost and water management, so that optimal production of rice plants could be achieved. The experiment was designed using a 2 × 4 factorials with 3 replications in a split plot design. The main plot was water management consists of 2 levels: continuous and intermittent irrigation (2 weeks flooded and 2 weeks field capacity). Small plot was humic acid which was extracted from rice straw compost by NaOH 0.5 N which consists of 4 levels: 0, 200, 400, and 600 mg kg-1.  The results showed that applications of humic acid from 0 to 600 mg kg-1 that was followed by 2 weeks of intermittent irrigation decreased Fe2+ concentration. It was approaching levels that were not toxic to plants, with soil Fe2+ between 180-250 mg kg-1. The best treatment was found at the application of 600 mg kg-1 humic acid extracted from rice straw compost combined with 2 week flooded – 2 weeks field capacity of water management. Those treatment decreased Fe2+ concentration from 1,614 to 180 mg kg-1 and increased the dry weight of grain from 5.15 to 16.73 g pot-1 compared to continuous flooding and without humic acid application.