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All Journal Journal of the Indonesian Tropical Animal Agriculture
T. P. Priyatno
Research Center for Animal Husbandry, National Research and Innovation Agency, Soekarno Science and Technology Park
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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A cost-effective non-enzymatic isolation method for bovine adipose-derived mesenchymal stem cells: A preliminary study for tropical livestock applications H. Zulfiqar; S. Suyatno; S. Irfan; D. A. Lestari; I. Rahmawati; A. Hafid; F. Saputra; P. S. Sushadi; I. M. Nainggolan; T. Kostaman; H. Herdis; T. P. Priyatno; M. Pangestu
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 2 (2026): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.2.125-134

Abstract

Animal-derived mesenchymal stem cells (MSCs) have potential applications in livestock systems, particularly in regenerative medicine and reproductive biotechnology. In tropical settings, the development of cost-effective and enzyme-free isolation methods is important to support research and applications under limited laboratory resources. This study aimed to preliminarily evaluate bovine adipose-derived mesenchymal stem cells (AD-MSCs) isolated using a non-enzymatic method, focusing on in vitro morphology, marker expression, chromosomal profile, and post-cryopreservation viability. Adipose tissue from Ongole Grade cattle was processed using an explant culture approach. The isolated cells exhibited fibroblast-like morphology, expressed CD44 and CD166, and lacked expression of the hematopoietic marker CD45. Flow cytometry analysis further confirmed CD44 expression at the protein level, with a high proportion of CD44-positive cells. Karyotype analysis showed that most cells retained the normal diploid chromosome number (2n = 60), although minor variations in chromosome morphology were observed. Post-thaw viability remained above 90%, with no observable decline when evaluated using the same cell populations. These findings provide preliminary evidence that non-enzymatic isolation can support the establishment and maintenance of bovine ADMSCs. This approach may serve as a cost-effective and practical alternative for MSC isolation and biobanking in tropical livestock research systems.
Co-Authors A. Hafid D. A. Lestari F. Saputra H. Herdis H. Zulfiqar I. M. Nainggolan I. Rahmawati M. Pangestu P. S. Sushadi S. Irfan S. Suyatno T. Kostaman