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D. A. Lestari
Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

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Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia E. T. Setiatin; D. A. Lestari; D. W. Harjanti; A. Setiaji; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.28-36

Abstract

The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
Effect of faecal microbiota transplantation on growth, haematological and intestinal indices of Kedu chickens M. R. Yousaf; A. N. M. Ramli; D. A. Lestari; S. Sutaryo; I. Agusetyaningsih; T. Ayasan; N. S. Pandupuspitasari; A. Setiaji; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 3 (2026): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.3.162-170

Abstract

The study examined the effect of lyophilized fecal microbiota transplantation (FMT) from healthy chicken donors on growth performance, haematological indices and intestinal morphology of Kedu chickens during the starter phase. A number of 120 Kedu chickens (0-6 weeks old) were randomly as-signed to T0 (orally inoculated with 1 mL PBS), T1 (inoculated with 0.5 mL FMT) and T2 (1.0 mL FMT). Body weight and feed intake were weekly recorded, while blood and intestinal segments were collected at week 6. Results showed that live body weight and body weight gain were highest (p<0.001) in T1, and the lowest in T0 group. Feed conversion ratio (FCR) was better (p=0.001) in T1 and T2 compared to T0 group. Feed intake was lower (p=0.014) in T2 than in T0 and T1 groups. The FMT administration tended to increase erythrocytes (p=0.054) and haemoglobin levels (p=0.056) of Kedu chickens. The relative weight of duodenum, jejunum, ileum, caecum and colon were lower (p<0.05) in T1 and T2 compared to those in T0 group. The FMT administration had no effect (p>0.05) on duodenal and jejunal villi height, crypt depth and the ratio of villi height to crypt depth of Kedu chickens. In conclusion, daily low-dose lyophilized FMT was associated with improved growth perfor-mance and feed efficiency in starter Kedu chickens, while haematological and intestinal morphological responses were limited.
Conditioned media and DMSO enhance the cryopreservation of bovine adipose tissue-derived mesenchymal stem cells S. Irfan; S. Suyatno; H. Zulfiqar; D. A. Lestari; A. Hafid; T. Kostaman; H. Herdis; T. P. Priyatno; P. I. Sitaresmi; M. F. Hudaya; F. B. I. Lupitasari; M. Pangestu
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 2 (2024): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.2.181-190

Abstract

Mesenchymal stem cells derived from adipose tissue (AD-MSCs) show great potential for repro ductive biotechnology in the livestock sector. However, enzyme-based isolation of MSCs is expensive and time-consuming, so it is still rarely done, especially for applications in the livestock sector in de veloping countries. So, MSCs must be cryopreserved with an efficient cryoprotective agent to be stored and reproduced in various laboratories after isolation. This study was aimed to optimize the cryopreser vation media for adipose-derived MSCs in cattle. This study evaluated the viability, proliferation, and morphology of AD-MSCs. The results of this study indicate that a combination of 10% DMSO, 45% DMEM, and 45% conditioned media significantly improves post-thaw viability, proliferation, and sur vival as compared to other mediums. Furthermore, AD-MSCs cryopreserved in this medium exhibit similar morphology as fresh cells. These findings suggest that the optimized cryopreservation medium can enhance the quality and safety of AD-MSCs for clinical applications in the livestock industry.
Genetic diversity and phylogenetic analysis of Indonesian local and commercial rabbit breeds based on mitochondrial D-loop D. A. Lestari; A. P. Latifa; S. Sutopo; E. Kurnianto; E. T. Setiatin; A. D. Agustine; Z. Nabilah; F. T. Kamila; P. G. Prahara; M. H. Kamalludin; A. Setiaji
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 3 (2025): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.3.189-198

Abstract

This study aimed to evaluate the genetic diversity and phylogenetic relationships among four rab-bit breeds raised in Central Java, namely Indonesian Local rabbit (LC), Hycole (HC), Hyla (HL), and New Zealand White (NZ), using mitochondrial D-loop region sequences. A total of 40 blood samples were collected from rabbits in three regencies in Central Java. DNA was extracted from each sample, followed by gene amplification, Sanger sequencing, and sequence analysis. A 906 bp fragment of the mitochondrial D-loop region was successfully amplified from all individuals. The overall genetic di-versity was found to be low, with mean values of k = 4.615, nucleotide diversity (π) = 0.00509 ±0.00092, and haplotype diversity (Hd) = 0.385 ± 0.070. Genetic distance analysis showed a close rela-tionship among the breeds, ranging from 0.00349 to 0.00610. Two haplotypes (A and B) were identi-fied, and both were shared across all breeds, indicating limited genetic differentiation. Phylogenetic tree reconstruction further demonstrated that the four breeds clustered closely together and were most likely derived from European lineages rather than distinct indigenous origins. In conclusion, rabbits raised in Central Java represent a genetically homogenous group with low diversity, highlighting the need for broader genetic resource management and conservation strategies to ensure long-term popula-tion sustainability.
A cost-effective non-enzymatic isolation method for bovine adipose-derived mesenchymal stem cells: A preliminary study for tropical livestock applications H. Zulfiqar; S. Suyatno; S. Irfan; D. A. Lestari; I. Rahmawati; A. Hafid; F. Saputra; P. S. Sushadi; I. M. Nainggolan; T. Kostaman; H. Herdis; T. P. Priyatno; M. Pangestu
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 2 (2026): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.2.125-134

Abstract

Animal-derived mesenchymal stem cells (MSCs) have potential applications in livestock systems, particularly in regenerative medicine and reproductive biotechnology. In tropical settings, the development of cost-effective and enzyme-free isolation methods is important to support research and applications under limited laboratory resources. This study aimed to preliminarily evaluate bovine adipose-derived mesenchymal stem cells (AD-MSCs) isolated using a non-enzymatic method, focusing on in vitro morphology, marker expression, chromosomal profile, and post-cryopreservation viability. Adipose tissue from Ongole Grade cattle was processed using an explant culture approach. The isolated cells exhibited fibroblast-like morphology, expressed CD44 and CD166, and lacked expression of the hematopoietic marker CD45. Flow cytometry analysis further confirmed CD44 expression at the protein level, with a high proportion of CD44-positive cells. Karyotype analysis showed that most cells retained the normal diploid chromosome number (2n = 60), although minor variations in chromosome morphology were observed. Post-thaw viability remained above 90%, with no observable decline when evaluated using the same cell populations. These findings provide preliminary evidence that non-enzymatic isolation can support the establishment and maintenance of bovine ADMSCs. This approach may serve as a cost-effective and practical alternative for MSC isolation and biobanking in tropical livestock research systems.
Gomperzt non-linear model for predicting growth performance of commercial broiler chickens A. Setiaji; D. A. Lestari; B. Ma&#039;rifah; L. Krismiyanto; I. Agusetyaningsih; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 2 (2023): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.2.143-149

Abstract

An experiment was conducted to estimate growth parameters for commercial broiler chickens in Indonesia. The data was collected from July 2021 to June 2022. A total of 1,570 samples consisting of four strains of broiler chickens were collected from 74 houses. The samples were daily weighed from 0 to 7 days of age, and they were weekly weighed from 1 to 5 weeks of age. A nonlinear Gompertz growth model was fitted to the observed body weights (BW). The results for five growth parameters were as follows: the asymptotic value (A) of the mature live weight ranged from 3.733 to 5.044 kg; the turning point of growth (B) ranged from 4.499 to 4.561; the value growth rate constant (K) ranged from 0.049 to 0.059 kg/week; Inflection points in ranged from 25.292 – 30.970 days, and 1.373 – 1.855 kg for inflection age (IA) and inflection weight (IW), respectively. The model was an excellent fit for the growth data in the commercial broiler with a low Akaike information criterion (AIC), and high coeffi-cient determination (R2).
Association analysis of the transforming growth factor factor-β2 gene with growth performance in Kedu chickens M. Akramullah; E. Kurnianto; D. A. Lestari; E. T. Setiatin; D. Y. Palupi; Y. N. Anjani; G. P. Dewi; A. Setiaji
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 1 (2026): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.1.72-81

Abstract

The Transforming Growth Factor-β2 (TGF-β2) gene is one of the cytokine genes that plays an important role in regulating growth processes and disease resistance. This study aimed to analyze the association between the TGF-β2 gene and growth performance in Kedu chickens. A total of 130 Kedu chickens, consisting of 72 males and 58 females, were observed for 10 weeks. Growth parameters measured included body weight, feed intake, body weight gain, and feed conversion ratio. Identifica-tion of the single nucleotide polymorphism (SNP) in the TGF-β2 gene was performed using the PCR-RFLP method. The association analysis between the g.640 T>C SNP polymorphism of the TGF-β2 gene and growth performance was conducted using analysis of variance under a General Linear Model (GLM). The results showed that the g.640 T>C SNP of the TGF-β2 gene was polymorphic and not in Hardy–Weinberg equilibrium. The TT genotype exhibited better growth performance (body weight gain, feed intake, and feed conversion ratio) than the CC genotype, particularly at 10 weeks of age in both female and unsexed chickens.
DNA variants and population structure of Magelang ducks across generation A. Febriana; E. Kurnianto; S. Sutopo; D. A. Lestari; A. Setiaji; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 1 (2024): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.1.67-77

Abstract

Magelang ducks (MD) are Indonesian local ducks and are known as dual-purpose type ducks which have high egg production, duck day production (DDP), and body weight size. Nowadays, the MD is selected to establish the pure line to advance the egg production trait in the Breeding and Rearing Center of Non-Ruminant Animals Banyubiru, Central Java. The mitochondrial DNA (mtDNA) Dloop region, is highly polymorphic and could be used to analyze the population genetics. The present research aims to examine the impacts of continuous selection on population structure and genetic mutations on MD across two generations using the mtDNA D-loop region. Thirty-six blood samples from the second (G2) and third (G3) generations were examined using the sequencing method. The MEGA X and DnaSP software were applied to calculate the genetic diversity, genetic distance, and to generate a phylogenetic tree. The number of haplotypes (H), haplotype diversity (Hd), and Tajima's D are 26, 0.9746, and -1.46, respectively. The Fst value of MD is 0.156. The genetic distance among populations ranges from 0.0000 – 2.097. The UPGMA analysis constructs one clade in a phylogenetic tree between MD, Indonesian local Ducks, Indian Ducks, Vietnamese Ducks, and Chinese Ducks. The study found that the genetic variation and population structure did not significantly change between the second and third generations.
Quantifying of morphological character for Kacang goat using principal component factor analysis D. A. Lestari; S. Sutopo; E. Kurnianto; M. I. A. Dagong; S. R. A. Bugiwati; K. Mamat-Hamidi; A. Yakubu; N. S. Pandupuspitasari; I. Agusetyaningsih; F. T. Kamila; A. Setiaji
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 4 (2024): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.4.316-322

Abstract

The study’s objective was to estimate the association among various linear body measurements and body weights of adult Kacang goats. The data was obtained from 209 heads of adult Kacang Goat, compressed 78 bucks and 131 ewes. The morphological evaluation was performed by measuring body weight (BW), body length (BL), chest depth (CD), chest girth (CG), chest width (CW), and withers height (WH). Factor PROCEDURE was performed to estimate the principal component. The result of factor analysis was used to determine the independent variable for linear regression analysis. BW has a favorable correlation with CG, BL, CD, CW, and WH for bucks and ewes. PC 1 accounts for 55.62% of the variation in bucks, while PC 2 accounts for an additional 18.34%. PC 1 accounts for just 0.45% of the overall variation in ewes, whereas PC 2 accounts for 0.24%. The R-squared (R2) values for bucks and ewes in the regression equation with CG as the independent variable are 0.32 and 0.41, respectively. For both bucks and ewes, the regression equation with CW as the independent variable had a higher R2 of 0.52 and 0.20, respectively. For bucks and ewes, the regression equation'sR2 values are 0.54 and 0.44, respectively, with combined CG and CW acting as independent variables. This integrated approach to analyzing body measurements in Kacang Goats provides a robust foundation for making informed decisions in goat farming.
Co-Authors A. D. Agustine A. Febriana A. Hafid A. Hafid A. N. M. Ramli A. P. Latifa A. Setiaji A. Setiaji A. Yakubu B. Ma&#039;rifah D. W. Harjanti D. Y. Palupi E. Kurnianto E. Kurnianto E. T. Setiatin E. T. Setiatin F. B. I. Lupitasari F. Saputra F. T. Kamila G. P. Dewi H. Herdis H. Herdis H. Zulfiqar H. Zulfiqar I. Agusetyaningsih I. Agusetyaningsih I. M. Nainggolan I. Rahmawati K. Mamat-Hamidi L. Krismiyanto M. Akramullah M. F. Hudaya M. H. Kamalludin M. I. A. Dagong M. Pangestu M. R. Yousaf N. S. Pandupuspitasari NURULIARIZKI SHINTA PANDUPUSPITASARI P. G. Prahara P. I. Sitaresmi P. S. Sushadi S. Irfan S. Irfan S. R. A. Bugiwati S. Sugiharto S. Sutaryo S. Sutopo S. Sutopo S. Suyatno S. Suyatno T. Ayasan T. Kostaman T. Kostaman T. P. Priyatno T. P. Priyatno Y. N. Anjani Z. Nabilah