Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal Journal of the Indonesian Tropical Animal Agriculture
M. Pangestu
Education Program in Reproduction and Development, Department of Obstetrics and Gynecology, Monash Clinical School, Monash University
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Conditioned media and DMSO enhance the cryopreservation of bovine adipose tissue-derived mesenchymal stem cells S. Irfan; S. Suyatno; H. Zulfiqar; D. A. Lestari; A. Hafid; T. Kostaman; H. Herdis; T. P. Priyatno; P. I. Sitaresmi; M. F. Hudaya; F. B. I. Lupitasari; M. Pangestu
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 2 (2024): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.2.181-190

Abstract

Mesenchymal stem cells derived from adipose tissue (AD-MSCs) show great potential for repro ductive biotechnology in the livestock sector. However, enzyme-based isolation of MSCs is expensive and time-consuming, so it is still rarely done, especially for applications in the livestock sector in de veloping countries. So, MSCs must be cryopreserved with an efficient cryoprotective agent to be stored and reproduced in various laboratories after isolation. This study was aimed to optimize the cryopreser vation media for adipose-derived MSCs in cattle. This study evaluated the viability, proliferation, and morphology of AD-MSCs. The results of this study indicate that a combination of 10% DMSO, 45% DMEM, and 45% conditioned media significantly improves post-thaw viability, proliferation, and sur vival as compared to other mediums. Furthermore, AD-MSCs cryopreserved in this medium exhibit similar morphology as fresh cells. These findings suggest that the optimized cryopreservation medium can enhance the quality and safety of AD-MSCs for clinical applications in the livestock industry.
A cost-effective non-enzymatic isolation method for bovine adipose-derived mesenchymal stem cells: A preliminary study for tropical livestock applications H. Zulfiqar; S. Suyatno; S. Irfan; D. A. Lestari; I. Rahmawati; A. Hafid; F. Saputra; P. S. Sushadi; I. M. Nainggolan; T. Kostaman; H. Herdis; T. P. Priyatno; M. Pangestu
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 2 (2026): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.2.125-134

Abstract

Animal-derived mesenchymal stem cells (MSCs) have potential applications in livestock systems, particularly in regenerative medicine and reproductive biotechnology. In tropical settings, the development of cost-effective and enzyme-free isolation methods is important to support research and applications under limited laboratory resources. This study aimed to preliminarily evaluate bovine adipose-derived mesenchymal stem cells (AD-MSCs) isolated using a non-enzymatic method, focusing on in vitro morphology, marker expression, chromosomal profile, and post-cryopreservation viability. Adipose tissue from Ongole Grade cattle was processed using an explant culture approach. The isolated cells exhibited fibroblast-like morphology, expressed CD44 and CD166, and lacked expression of the hematopoietic marker CD45. Flow cytometry analysis further confirmed CD44 expression at the protein level, with a high proportion of CD44-positive cells. Karyotype analysis showed that most cells retained the normal diploid chromosome number (2n = 60), although minor variations in chromosome morphology were observed. Post-thaw viability remained above 90%, with no observable decline when evaluated using the same cell populations. These findings provide preliminary evidence that non-enzymatic isolation can support the establishment and maintenance of bovine ADMSCs. This approach may serve as a cost-effective and practical alternative for MSC isolation and biobanking in tropical livestock research systems.
Co-Authors A. Hafid A. Hafid D. A. Lestari F. B. I. Lupitasari F. Saputra H. Herdis H. Herdis H. Zulfiqar H. Zulfiqar I. M. Nainggolan I. Rahmawati M. F. Hudaya P. I. Sitaresmi P. S. Sushadi S. Irfan S. Irfan S. Suyatno S. Suyatno T. Kostaman T. Kostaman T. P. Priyatno T. P. Priyatno