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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati LIMNOTEK - Perairan Darat Tropis di Indonesia LIMNOTEK Annales Bogorienses
Chrismadha, Tjandra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Engineering Environmental Science

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Pengaruh Jarak Penyekat dalam Fotobioreaktor Tubular terhadap Pertumbuhan Kultur Mikroalga Ankistrodesmus convulutus Chrismadha, Tjandra; Rustiani, Tenni; Rosidah, Rosidah; Mardiati, Yayah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.514 KB) | DOI: 10.24002/biota.v12i1.2530

Abstract

A series of partition was constructed inside a vertical tubular photobioreactor to control the culture distribution in obtaining intermittent exposure to light area in the surface of the tubular column. A microalga, Ankistrodesmus convulutus, was grown in a batch mode in the reactor with PHM medium, light sources of fluorescent lamp 3 x 40 watt (I = 5.500 lux on the surface of the tubular) and room temperature of 27 – 310C. The experiment was carried out to study the influence of partition density which was constructed at varied distance, which were 1, 2 and 3 inch on the growth and biochemical composition of the alga. A vertical tubular photobioreactor without partition was applied as the control. The partition density did not significantly affect the optical density achievement of the culture, but it significantly affected the biomass and chlorophyll concentration, as well as the protein/carbohydrate ratio of the culture. It is believed that the intermittent exposure of algal cell to the light area leads to sincronization of the light and dark reaction of the photosynthesis, as well as to vapor the catalitic proccesses to develop the functional cell structure.
PENYISIHAN NUTRIEN DENGAN KULTUR Eichornia Crassipes DALAM AIR LIMBAH KOLAM IKAN RESIRKULASI SEMI TERTUTUP Satya, Awalina; Chrismadha, Tjandra; Sulawesty, Fachmijany; Yoga, Gunawan P.; Mardiyati, Yayah
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 2 (2014)
Publisher : Research Center for Limnology

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Abstract

Air Limbah kolam ikan resirkulasi memiliki karakteristik yang mirip dengan perairan eutrofik sehingga tidak aman untuk dibuang ke perairan umum secara langsung. Pada penelitian ini dilakukan evaluasi kemampuan tiga variasi kepadatan eceng gondok (Eichornia crassipes) untuk memperbaiki kualitas air limbah kolam ikan resirkulasi tersebut. Tujuan penelitian adalah untuk 1) mengungkapkan dinamika kualitas air dalam ?batch culture? 2) kinetika laju reduksi nutrien nitrogenik-fosforik dan konstituen pencemar lainnya, dan 3) efektifitas penyisihan nutrien. Eksperimen terdiri atas empat bak plastik, B1, B2, B3 dan B4. Bak B1 merupakan kontrol, hanya berisi air limbah tanpa eceng gondok. Bak B2, B3 dan B4 berisi eceng gondok dengan kepadatan awalnya berturut-turut adalah 1.618,40 gram/m2; 2.436,51 gram/m2 ; dan 3.243,93 gram/m2. Percobaan dilakukan selama empat hari. Pengukuran pH,Oksigen terlarut (Dissolved Oxygen,DO), konduktifitas, suhu, Total Dissolved Solid (TDS), dan % DO saturation dilakukan tiga kali sehari pada jam 09.00-09.30 ; 12.00-12.30 dan 16.00-16.30, sedangkan Senyawa nutrien nitrogenik (N-NH3+; N-NO2-; N-NO3-, Total Nitrogen (TN)), fosfor (TP), Total Suspended Solid (TSS) dan Total Organic Matter (TOM) dianalisis dua kali sehari pada pagi dan sore hari. Reduksi parameter konduktifitas,TDS, nutrien, nitrogenik, dan fosforik dalam bak-bak yang ditumbuhi eceng gondok mengikuti model kinetika order pertama. Kepadatan eceng gondok ideal adalah 2.436,51 gram/m2 (bak B3) karena menghasilkan pertambahan densitas yang paling tinggi (147,13 gram/m2). Bak berisi eceng gondok paling efektif menyisihkan turbiditas (94,28%-100%), N-nitrit (98,21%-98,93%), TP (92,86%-93,62%), N-nitrat (58,33%-83,33%), TN (59,46%-66,06%), N-ammonia (18.82%-46,88%) dan konduktifitas (16,34%-23,54%). Seluruh perlakuan dan kontrol terbukti tidak efektif untuk menyisihkan material organik.
LAJU PERTUMBUHAN IKAN MAS (Cyprinus carpio L) DENGAN PEMBERIAN PAKAN LEMNA (Lemna perpusilla TORR.) SEGAR PADA KOLAM SISTEM ALIRAN TERTUTUP Sulawesty, Fachmijany; Chrismadha, Tjandra; Mulyana, Endang
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 21, No 2 (2014)
Publisher : Research Center for Limnology

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Abstract

Penelitian telah dilakukan untuk meningkatkan kinerja budidaya perikanan melalui pemanfaatan pakan alternative yang murah dan ramah lingkungan. Pakan alternatif yang dipilih adalah jenis tumbuhan air, lemna (Lemna perpusilla Torr) yang dikultur secaraintegratif pada suatu sistem produksi perikanan sehingga mempunyai fungsi sebagai sumberpakan alami sekaligus menyerap bahan pencemar kegiatan budidaya. Pengamatan inibertujuan untuk melihat pengaruh pemberian lemna segar terhadap laju pertumbuhan ikan mas(Cyprinus carpio L) pada kolam sistem aliran tertutup. Biomassa lemna segar digunakan untukmengantikan pellet pada porsi setara 50% dari berat kering pakan yang diberikan pada ikanmas sebanyak 3% dari berat badannya per hari. Pengamatan dilakukan selama 24 minggu.Ukuran berat awal ikan mas rata-rata 4,75 - 6,5 g dngan padat penebaran100 ekor per wadah.Parameter pengamatan adalah berat rata-rata ikan,, laju pertumbuhan spesifik, rasio konversipakan, faktor kondisi, dan tingkat kelangsungan hidup ikan. Pemberian campuran lemna segarpada pakan ikan mas memberikan nilai laju pertumbuhan spesifik lebih tinggi, yaitu sebesar2,00 %/hari, dibandingkan dengan yang hanya diberi pakan pellet (1,75 %/hari). Berat ratarata,nilai konversi pakan, dan tingkat kelangsungan hidup ikan yang diberi pakan campuranlemna segar bertutut-turut: 162,7 g, 2,00, dan 64 %, lebih tinggi dibandingkan dengan ikanyang diberi ransum pellet saja (berturut-turut 108,9 g, 3,34, dan 61 %). Pertumbuhan ikanbersifat allometrik negatif (nilai b < 3) dengan nilai b pada ikan yang diberi pakan campuranpellet dan lemna segar lebih tinggi (2,737) dibandingkan dengan ikan yang diberi pakan pelletsaja (2,537). Hasil ini mengindikasikan adanya pengaruh positif pemberian lemna segar yaitudapat meningkatkan pertumbuhan ikan mas. Pemanfaatan biomassa lemna segar untukcampuran pakan ikan mas dapat meningkatkan efisiensi produksi pada usaha budidaya ikanmas.
Growth of the water fleas Daphnia magna (Straus, 1820) at different trophic levels of two small urban lakes in Indonesia Tanjung, Livia Rossila; Chrismadha, Tjandra; Nafisyah , Eva
LIMNOTEK Perairan Darat Tropis di Indonesia Vol. 29 No. 1 (2023)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/limnotek.2023.1099

Abstract

Nutrient enrichment in waters that has become a major environmental problem is related to excessive loading of nutrients into aquatic ecosystems. This nutrient enrichment, called eutrophication, favors phytoplankton growth, which can function as a natural daphnid feed. This study examined the growth performance of the water fleas Daphnia magna in water collected from small lakes (ponds) of different trophic levels. The water was taken from Situ Rawa Kalong, considered eutrophic from its dark green color, and the less eutrophic Situ Cibuntu with relatively clear water. Daphnids were grown in six aquaria filled with water from both ponds without artificial feeding with an initial density of five individuals/L. Samples of daphnids were taken every three to four days to observe their growth and reproduction, along with water samples to analyze the chlorophyll content and total suspended solids (TSS). The result showed that the eutrophic water of Situ Rawa Kalong favored phytoplankton growth, indicated by a consistently higher chlorophyll content in the water ranging from 35.3 to 140.7 μg/L compared to less eutrophic water of Situ Cibuntu with chlorophyll content ranging from 1.4 to 13.2 μg/L throughout the experiment. A much higher daphnid density of 151.7 individuals/L was achieved with more water chlorophyll content, meaning phytoplankton availability became a controlling factor for daphnid growth in the pond waters. This study reveals the functional relationships in the food chain between the water trophic level, the abundance of phytoplankton as the primary producer, and daphnids as the first-order predator. It also suggests that the open water trophic level can be managed to favor the daphnid growth, which can then be harvested for use as natural feed.
Effect of Light Intensity on Ammonium Removal and Biomass Growth in Different Levels of Aquaculture Effluent Using Duckweed (Lemna perpusilla) Agus Waluyo; Nirmala, Kukuh; Satya, Awalina; Puji Hastuti, Yuni; Chrismadha, Tjandra; Susanti, Evi; Sumi Lestari, Fajar; Nafisyah, Eva; Sugiarti; Nasrul, Muit
LIMNOTEK Perairan Darat Tropis di Indonesia Vol. 30 No. 2 (2024)
Publisher : BRIN Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/limnotek.2024.6420

Abstract

Cultivating duckweed in aquaculture effluent offers a viable approach to eliminating contaminants. The duckweed biomass obtained can be utilized for the generation of bioenergy. However, elevated levels of ammonium (NH4+) in aquaculture effluent, combined with variations in light intensity, can hinder biomass formation. The precise mechanisms underlying this inhibition remain incompletely elucidated. The study assessed the efficacy of duckweed (Lemna perpusilla) as a treatment agent for wastewater from catfish farms. The objective was to evaluate the growth response of duckweed and its efficacy in reducing ammonium levels. The research demonstrated that daily light intensity fluctuated using shade nets and that the ammonium concentration of aquaculture wastewater varied according to the age of the fish. The shade nets, which blocked 25% of the sunlight and had an average daily light intensity of 3433.34–15199.56 lux, demonstrated a slightly elevated NH4+ removal efficiency and duckweed productivity of 69.34% and 0.050 kg/m²/day, respectively. However, these values were not statistically significant when compared to conditions without shade nets, which had a removal efficiency of 63.97% and duckweed productivity of 0.042kg/m2/day (P<0.05). The implementation of shade structures that effectively decrease solar exposure by 25% shows promise for enhancing duckweed productivity and optimizing nutrient reduction in wastewater from fish cultivation systems. This approach contributes to the promotion of sustainable integrated aquaculture.
The Influence of Harvesting Period on Lipid Associated Antioxidant Activity of Semicontinuously Grown Chlorella vulgaris Chrismadha, Tjandra; Sartika, Diani; Setyaningsih, Iriani; Uju, Uju
Annales Bogorienses Vol. 14 No. 1 (2010): Annales Bogorienses
Publisher : BRIN

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Abstract

A green alga, Chlorella vulgaris, was grown semi-continuously at various harvesting periods, and the lipid content and its associated antioxidant activity was examined. The harvesting periods were 9, 18, and 27 days, while the algal culture was placed in 10 L glass bottles provided with aeration for gas exchange and mixing. Light source was obtained from 440 watt cool fluorescent lamps placed at one side of the culture. Room temperature was 26-32C. Algal lipid extraction was done based on liquid phase separation ofmethanol:chloroform:water and the antioxidant activity was examined by means of oxidation inhibitory in ethanol emulsified limnoleic acid. In addition, a TLC analysis was performed to identify the antioxidant compounds soluble in the lipid. The results showed that harvesting period had a significant influence on the algal lipid content, which were 11.94, 12.96, and 16.51% of the dry weight in the culture with harvesting periods of 9, 18, and 27 days, respectively. No remarkable effect of the harvesting period on the antioxidant activity, which were observed to inhibit oxidation of linoleic acid up to 67-71%. There were five compounds found can be associated with the algal antioxidant activity, which were pheophorbide-a, chlorophyll-b, chlorophyll-a, phaeophytin-a, -carotene, and an unidentified one.
Growth and Phycocyanin Productitivity of Spirulina fusiformis under Various Light Regimes Chrismadha, Tjandra; Waluya, Rizky Agus
Annales Bogorienses Vol. 17 No. 1 (2013): Annales Bogorienses
Publisher : BRIN

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Abstract

A blue green algae
Co-Authors Agus Waluyo Endang Mulyana Evi Susanti Gunawan P. Yoga, Gunawan P. Kukuh Nirmala Mardiati, Yayah Mardiyati, Yayah Nafisyah , Eva Nafisyah, Eva Nasrul, Muit Puji Hastuti, Yuni Rosidah Rosidah Rustiani, Tenni Sartika, Diani Satya, Awalina Sugiarti Sulawesty, Fachmijany Sumi Lestari, Fajar Tanjung, Livia Rossila Uju Uju Waluya, Rizky Agus