The community service program conducted by Universitas Flores in Wolomuku and Djeodua Villages, Ende Regency, aimed to support spring conservation and river ecosystem restoration through a participatory-collaborative approach. The program was implemented as part of the university’s Green University and Kampus Berdampak initiatives, involving the Tana Nua Flores Foundation, village governments, traditional leaders, local communities, and approximately 300 lecturers and educational staff. The activities consisted of environmental problem identification, planning, planting 3,165 productive tree seedlings comprising durian and nutmeg, releasing shrimp juveniles into two rivers at the activity sites, environmental education, literacy and numeracy activities, health services, and monitoring and evaluation. The results demonstrated collaborative efforts in vegetation conservation and aquatic ecosystem restoration, while strengthening the engagement of the academic community and local residents in environmental sustainability. The planting of productive trees provided ecological benefits while offering potential long-term economic benefits for local communities, whereas the release of shrimp juveniles served as an educational medium for promoting awareness of river ecosystem protection. Overall, the program demonstrates that a participatory-collaborative approach can integrate environmental conservation, community empowerment, education, and social services into a comprehensive community service program.