Santi, M. A., Nurhayati, N., Suryani, H., Maradon, G. G., Sinaga, D. M., Pertiwi, V. R., Elvan, E., & Karima, F. N. (2025). Profil Flavonoid Buah Andaliman (Zanthoxylum acanthopodium): Kajian Fitokimia untuk Pengembangan Feed Additive Alami pada Unggas. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-13 Tahun 2025, Palembangg 20 Oktober 2025. (pp. x–y). Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Andaliman fruit (Zanthoxylum acanthopodium) is a spice plant native to North Sumatra that contains bioactive compounds, particularly flavonoids, which have the potential to be used as natural feed additives to improve poultry performance and health. This study aimed to identify the flavonoid profile in andaliman fruit extract as a basis for developing natural feed additives. The extraction process was carried out using the maceration method with distilled water for 48 hours, followed by evaporation using a hot plate at 60-70°C for 6 hours. Analysis was performed through qualitative testing, determination of total flavonoid content, and comparison with the quercetin standard curve. The results showed that andaliman fruit extract contained flavonoid compounds, as indicated by an intense color change in the phytochemical test. The quercetin standard curve showed good linearity (R2=0.996), with the total flavonoid content of andaliman extract reaching a significant value, indicating its high potential as a natural antioxidant source. The chromatogram profile showed the presence of quercetin and its derivatives as the dominant components. These results reinforce the evidence that andaliman fruit is a source of important bioactive compounds that can be developed as natural-based poultry feed additives to support livestock health and performance. Thus, andaliman fruit extract has the potential to be an alternative to synthetic additives in functional poultry feed formulations.