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All Journal JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Agrokompleks
Rizkie, Lilian
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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KERAPATAN DAN VIABILITAS KONIDIA BEAUVERIA BASSIANA DAN METARHIZIUM ANISOPLIAE PADA MEDIA IN VITRO PH RENDAH Rizkie, Lilian; Herlinda, Siti; ., Suwandi; Irsan, Chandra; ., Susilawati; Lakitan, Benyamin
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.83 KB) | DOI: 10.23960/j.hptt.217119-127

Abstract

Conidial density and viability of Beauveria bassiana and Metarhizium anisopliae grown on the low-pH in vitro medium. Liquid bioinsecticide with active ingredient from conidial entomopathogenic fungus has major constraints, namely short shelf life due to declining conidial viability and density is caused by low pH in the bioinsecticide carrier. This experiment aimed to measure the loss of conidial viability and density of Beauveria bassiana and Metarhizium anisopliae isolates grown on in vitro medium with low pH. Entomopathogenic fungus isolates were used as much as 28 isolates grown on in vitro medium at low pH, namely pH 5, 4, 3, and 2. The results showed that the fungus isolate that had the highest conidial density on in vitro medium at pH 5 was found on isolates of B. bassiana with code BPcMs (2.583 x 109 conidia mL-1), while the lowest one was found on isolates of B. bassiana with code of BWS Pantura (0.825x109 conidia mL-1). All isolate conidial density from in vitro medium with pH 2 decreased regularly. Conidial density of BPcMs isolate decreased to 2.483 x 109conidia mL-1, as well as BWS Pantura isolate also decreased to 0.425x109 conidia mL-1. The highest conidial viability at pH 5 was found on isolates of B. bassiana with code of BPcMs (51.572%), while the lowest conidial viability was found on isolate of B. bassiana with BTmPc code (15.040%). At pH 2, almost isolates tested had low conidial viability. The conidial viability of isolates BPcMs decreased to 47.037%%, while the isolates BTmPc also decreased to 12.778%. Therefore, the lower of the pH of the in vitro medium was, the lower of conidial viability and density of B. bassiana and M. anisopliae was
Pengaruh ekstrak n-heksan daun pepaya (Carica papaya) terhadap aktivitas biologis ulat grayak jagung (Spodoptera frugiperda J. E. Smith) Silahudin, Imam Muhamad Alam; Ramadhan, R. Arif Malik; Rizkie, Lilian
Agrokompleks Vol 25 No 1 (2025): Agrokompleks Edisi Januari
Publisher : Politeknik Pertanian Negeri Pangkajene Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51978/japp.v25i1.794

Abstract

Insektisida sintetik cukup efektif dalam mengendalikan larva Spodoptera frugiperda, akan tetapi terdapat berbagai dampak negatif bagi lingkungan, manusia, dan terjadinya resistensi OPT. Carica papaya memiliki kandungan metabolit sekunder yang bersifat insektisidal. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) non faktorial terdiri dari 5 taraf perlakuan di antaranya 0%, 0,05%, 0,1%, 0,2%, 0,4%, dan 0,8% ekstrak daun pepaya. Hasil penelitian menunjukan bahwa senyawa-senyawa yang terkandung dalam ekstrak n-heksan daun C. papaya di antaranya alkaloid, steroid, dan terpenoid. Ekstak n-heksan daun papaya berpengaruh nyata terhadap mortalitas larva secara kumulatif pada 23 hari setelah aplikasi (HSA), mortalitas yang disebabkan oleh pemberian ekstrak n-heksan daun C. papaya masih kurang efektif yaitu masih di bawah0%. Pada perkembangan instar II-VI menunjukan perlakuan 0,8% lebih lambat 8,93 hari dibandingkan dengan perlakuan kontrol.
Siklus hidup dan kesintasan ulat grayak Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) dari beberapa daerah di Jawa Barat: Life cycle and survivorship rate of fall armyworm Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) from several areas in West Java Susanto, Agus; Leider, Putri Artha; Maharani, Yani; Rizkie, Lilian; Bari, Ichsan Nurul; Subakti-Putri, Syifa Nabilah
Jurnal Entomologi Indonesia Vol 21 No 2 (2024): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.2.167

Abstract

Spodoptera frugiperda Smith is a major pest of corn plants. Information on the life cycle and survival of this pest is important for proper and effective control. This study was conducted to determine the life cycle and survival of S. frugiperda in several areas in West Java. This study was conducted from June to August 2020. Samples were taken from several areas divided into three categories: highlands in Lembang District (1,100 m asl), middlelands in Jatinangor District (858 m asl), and the lowlands in Tarikolot District (103 m asl). Sampling used the observation method to study the effect of differences in altitude on population, life cycle, and survival. The results showed that there was no significant difference in the development time of S. frugiperda among the three different areas. The average development time of S. fugiperda was 24.55 days (highlands), 24.36 days (middlelands), and 24.07 days (lowlands). The survival percentage of S. frugiperda from highland, middle, and lowland areas in the egg phase was 95.44%, 97.34%, and 96.89%, larval phase 98.46%, 98.65%, and 98.48%, and pupal phase 87.63%, 88.08%, and 93.43%. The sex ratio of S. frugiperda from the highlands was 1:1, from the middlelands was 1:1.28, and from the lowlands was 1:0.89. Location did not affect the width parameter of the head capsule in S. frugiperda. Based on the test results, it is known that the life cycle, survival, and sex ratio of S. frugiperda from several regions do not have significant differences.
Co-Authors Agus Susanto Bari, Ichsan Nurul Benyamin Lakitan Chandra Irsan Leider, Putri Artha R. Arif Malik Ramadhan Silahudin, Imam Muhamad Alam SITI HERLINDA Subakti-Putri, Syifa Nabilah Susilawati . Suwandi . Yani Maharani, Yani