<![CDATA[Background: The decline in semen quality during storage remains one of the major challenges in artificial insemination programs in pigs. Reduced sperm motility due to oxidative stress can limit reproductive success. Pineapple (Ananas comosus) juice contains bromelain and vitamin C, which function as natural antioxidants capable of protecting the sperm plasma membranes from oxidative damage. Purpose: This study aimed to evaluate the potential of pineapple juice as a natural additive in boar semen extender to improve sperm quality, particularly motility, viability, abnormality, and pH. Methods: Semen was collected from one healthy two-year-old boar, which was provided commercial feed at 3 kg/day with water offered ad libitum. The boar was previously trained for semen collection, which was carried out twice per week. The basic extender consisted of distilled water, glucose (20 g), sodium citrate (3.6 g), sodium bicarbonate (1.2 g), and EDTA (2 g) per liter. A Completely Randomized Design (CRD) was applied with four treatments: P0 (0% pineapple juice), P1 (2%), P2 (3%), and P3 (4%), each with four replications. Evaluated parameters included sperm motility, viability, abnormality, and pH. Data were analyzed using ANOVA followed by Tukey’s post hoc test. Results: Supplementation of pineapple juice had a highly significant effect (P<0.01) on sperm motility, with P3 (4%) yielding the highest value (83.34%). No significant effects were observed on viability, abnormality, or pH; however, all parameters remained within normal physiological ranges. The improved motility is presumed to be associated with the antioxidant action of vitamin C and bromelain, which contribute to enhanced metabolic activity and membrane stability. Conclusion: The addition of pineapple juice up to 4% in semen extender improves sperm motility without compromising other quality parameters, indicating its potential as a natural and economical semen extender innovation to enhance reproductive efficiency in pigs.]]>
