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All Journal SAINTIFIK@ : Jurnal Pendidikan MIPA Journal of Community Health Provision Jurnal Analis Kesehatan Kendari
Sernita, Sernita
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Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Anticancer Assay of Methanol Extract of Gracilaria salicornia Originating from the Hari Islands, Southeast Sulawesi, Against MCF-7 Cancer Cells Sernita, Sernita; Taba, Paulina; Sahidin, I; Soekamto, Nunuk Hariani
Journal of Community Health Provision Vol. 5 No. 1 (2025): Journal of Community Health Provision
Publisher : PSPP JOURNALS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55885/jchp.v5i1.553

Abstract

Marine macroalgae represent a promising but underexplored resource for anticancer drug discovery due to their diverse repertoire of bioactive secondary metabolites. Gracilaria salicornia, a red alga found abundantly in the waters surrounding the Hari Islands, Southeast Sulawesi, has not been previously studied for its cytotoxic potential. This study aimed to evaluate the phytochemical composition and anticancer activity of methanol extract from G. salicornia against MCF-7 human breast cancer cells. The algae were extracted using a gradient solvent system, with methanol selected for further testing due to its polarity and broad extraction capacity. Phytochemical screening revealed the presence of alkaloids, phenolics, and steroids/terpenoids—classes of compounds known for their cytotoxic, pro-apoptotic, and redox-modulatory properties. General toxicity was assessed using the Brine Shrimp Lethality Test (BSLT), with the extract demonstrating moderate toxicity (LC₅₀ = 561.26 mg/L), indicating the presence of bioactive constituents. Cytotoxicity against MCF-7 cells was evaluated using the MTT assay, yielding an IC₅₀ value of 414.6 mg/L. Although lower than the standard chemotherapeutic agent doxorubicin (IC₅₀ = 4.1 mg/L), the observed activity supports the hypothesis that G. salicornia contains compounds with therapeutic relevance. These findings suggest that G. salicornia harbors cytotoxic agents that act through diverse mechanisms, likely involving oxidative stress and hormonal regulation. While the crude extract exhibited only moderate activity, its phytochemical richness warrants further fractionation, isolation, and mechanism-of-action studies. This study provides foundational evidence for the potential of Indonesian marine algae in cancer drug discovery and highlights the need for continued exploration of native marine biodiversity as a source of novel chemotherapeutic agents.
Pengaruh Jenis Pelarut terhadap Aktivitas Antioksidan Ekstrak Daun Ubi Ungu (Ipomea batatas L.) sernita, sernita; Rubak, Boni; Srimayona, Wa Ode
Jurnal Analis Kesehatan Kendari Vol. 6 No. 2 (2024): Jurnal Analis Kesehatan Kendari (JAKK) : Vol. 6 (2) Juni 2024)
Publisher : Program Study of Medical Laboratory Technology , Politeknik Bina Husada Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46356/jakk.v6i2.304

Abstract

ABSTRACT Ethanol extract of purple sweet potato leaf contained secondary metabolite components, flavonoid and tannin. Flavonoids are polyphenol compounds that are widely distributed in plants as glycosides which bind to a sugar, therefore flavonoids are polar compounds. Polar solvents include methanol, ethanol, acetone and water. This research was conducted to determine the effect of extractor variation on the activity of antioxidant activity in purple potato sweet leaf extract (Ipomea batatas L.) Antioxidant activity of methanol extract, ethanol extract and acetone extract of purple sweet potato leaf was determined using DPPH method and vitamin C as a comparison with three replications. The results of the antioxidant activity determining showed that the average IC50 (Inhibitory Concentration) value of methanol extract was 52,52 mg/L; ethanol extract 30,82 mg/L; acetone extract was 9,86 mg/L and Vitamin C was 6.24 mg/L. Based on the results of One Way ANOVA and LSD analysis, the extractor variance give a significant effect on the antioxidant activity of purple sweet potato leaf extract. Keywords:Purple sweet Potatoes Leaf, Methanol Extract, Etanol Extract, Acetone Extract, DPPH. ABSTRAK Ekstrak etanol daun ubi ungu positif mengandung komponen metabolit sekunder golongan flavonoid dan tannin. Flavonoid merupakan senyawa golongan polifenol yang terdistribusi luas pada tumbuhan dalam bentuk glikosida yang berikatan dengan suatu gula, karena itu flavonoid merupakan senyawa yang bersifat polar. Pelarut yang bersifat polar diantaranya metanol, etanol, aseton dan air. Penelitian ini dilakukan untuk mengetahui pengaruh jenis pelarut terhadap aktivitas senyawa antioksidan dalam ekstrak daun ubi ungu (Ipomea batatas L.). Aktivitas antioksidan pada ekstrak metanol, ekstrak etanol dan ekstrak aseton daun ubi ungu diuji dengan menggunakan metode DPPH serta vitamin C sebagai pembanding dengan tiga kali replikasi. Hasil uji aktivitas antioksidan menunjukkan nilai IC50 (Inhibitory Concentration) rata-rata ekstrak metanol daun ubi ungu sebesar 52,52 mg/L; ekstrak etanol daun ubi ungu sebesar 30,82 mg/L; ekstrak aseton daun ubi ungu sebesar 9,86 mg/L dan pembanding Vitamin C sebesar 6,24 mg/L. Berdasarkan hasil analisis One Way ANOVA dan Uji BNT, jenis pelarut memberikan pengaruh yang signifikan terhadap aktivitas antioksidan ekstrak daun ubi ungu. Kata Kunci: Daun Ubi Ungu, Antioksidan, Ekstrak Metanol, Ekstrak Etanol, Ekstrak Aseton, DPPH
PENAPISAN FITOKIMIA EKSTRAK METANOL AKAR Gofasa (Vitex Cofassus) asal Pulau Halmahera DAN POTENSINYA SEBAGAI ANTIOKSIDAN dan ANTIKOLESTEROL Khadijah, Khadijah; merlin, Merlin; Baturante, Nurjannah; sernita, Sernita; Sumarna, Sabir; Jayali, Ahmad Muchsin
SAINTIFIK@: Jurnal Pendidikan MIPA Vol 9, No 1 (2024): SAINTIFIK@: Jurnal Pendidikan MIPA EDISI MARET 2024
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/saintifik.v9i1.8399

Abstract

Vitex cofassus (Gofasa) merupakan salah satu tanaman tropika yang secara tradisional telah digunakan sebagai bahan obat. Skrining  fitokimia, uji antioksidan, antikolesterol dan uji toksisitas terhadap A. salina terhadap ekstrak metanol Vitex cofassus  telah dilakukan. Ekstraksi komponen kimia dilakukan dengan cara maserasi dengan pelarut metanol dan dilakukan uji fitokimia terhadap ekstrak untuk mengetahui komponen kimianya. Uji kolesterol dilakukan dengan menggunakan metode CHOD-PAP dan uji antioksidan dilakukan dengan metode DPPH.  Hasil  skrining fitokimia menunjukkan ekstrak metanol akar V. cofassus mengandung alkaloid, terpenoid, flavonoid dan tanin. Ekstrak metanol akar V. cofassus memiliki kemampuan untuk menurunkan kolesterol sebanyak 92% dan memiliki nilai IC50 terhadap DPPH sebesar  65.043 µg/mL. Hal ini menunjukkan ekstrak metanol akar V. cofassus memiliki kemampuan sebagai antikolesterol dan antioksidan.
Co-Authors Ahmad Muchsin Jayali Baturante, Nurjannah Khadijah Khadijah Merlin Merlin, Merlin Nunuk Hariani Soekamto Paulina Taba Rubak, Boni Sahidin . Srimayona, Wa Ode Sumarna, Sabir