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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Kelautan Tropis Jurnal Laot Ilmu Kelautan
Nursalim, Nining
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences

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POTENSI NANNOCHLOROPSIS OCULATA DAN TETRASELMIS CHUII SEBAGAI BAHAN BAKU BIOETANOL Negara, Bertoka Fajar; Irfandi, Irfandi; Nursalim, Nining; Herliany, Nurlaila Ervina
Jurnal Laot Ilmu Kelautan Vol 2, No 2 (2019): Jurnal Laot Ilmu Kelautan
Publisher : Jurusan Ilmu Kelautan FPIK Universitas Teuku Uma

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.636 KB)

Abstract

Bahan bakar fosil yang semakin menipis menimbulkan permasalahan baru dalam mewujudkan ketahanan energi didunia. Bioetanol merupakan salah satu bahan bakar alternatif yang ramah lingkungan dan bersifat terbarukan. Penelitian ini bertujuan untuk memperoleh jumlah bioetanol yang dihasilkan dari fermentasi biomassa Nannochloropsis oculata dan Tetraselmis chuii. Kultur Nannochloropsis oculata dan Tetraselmis chuii selama 5 hari, hidrolisis asam dilakukan dengan menggunakan H2SO4 0,2 M dengan suhu121 oC dan tekanan 1 atm selama 30 menit, fermentasi menggunakan Saccaromyces cereviciae selama 5 hari. hasil hidrolisis Nannochloropsis oculata memiliki kadar gula sebesar 4%, hasil fermentasi Nannochloropsis oculata mengandung etanol sebanyak 12 mL dengan konsentrasi 4%. Hasil hidrolisis yang dilakukan menghasilkan kadar gula 4%. Hasil fermentasi menghasilkan 1% bioetaniol.
DNA Barcoding of Anchovy in Tuban Regency as Database of Indonesian Marine Genetic Diversity Joesidawati, Marita Ika; Nursalim, Nining; Kholilah, Nenik; Kurniasih, Eka Maya; Cahyani, Ni Kadek Dita; Ambariyanto, Ambariyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 4 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.4.383-391

Abstract

Anchovy is the main catch and the primary consumption of coastal communities in Indonesia, and its production shows an increase of more than 10% in 2021. Tuban district, in East Java, Indonesia is part of the WPP 712 (Wilayah Pengelolaan Perikanan or Fisheries Management Area) and highly produces anchovies’ fisheries. Anchovy has a small size, making it difficult to identify morphologically. This study aimed to genetically identify anchovy samples obtained from North Java (Tuban) waters. Molecular identification was conducted by utilizing Cytochrome Oxidase Subunit I (COI) gene using jg-HCO and jg-LCO primers. This study observed 12 individual samples with 623 base pair sequence length. Five species were obtained, namely four species of anchovies (Encrasicholina heteroloba, Encrasicholina punctifer, Stolephorus waitei, and Stolephorus insularis) and one species of sardines (Dussumieria elopsoides) with 99.84-100% similarity to NCBI sequences data. Anchovies typically have a streamlined body with a slightly compressed shape. Anchovies have cycloid scales, which are smooth-edged and relatively small, ranging from a few centimeters to around 20 centimeters in length. Some of the genus from the Anchovy group are Encrasicholina and Stolephorus. The phylogenetic tree reconstruction leads into four clades with a genetic distance between clades of 17,9-24,5 %. This research provides methods and data on the genetic diversity of anchovies taxa caught in Tuban, East Java. The findings are expected to support promoting new standards for healthier and more sustainable anchovy stocks in the country. Overall, this study contributes to providing valuable insights for fisheries management and conservation efforts in Indonesia.
Unveiling the DNA Barcoding of Threadfin Breams (Nemipteridae) at Oeba Fish Landing Site and Oesapa Fish Market in Kupang, East Nusa Tenggara Wora, Umbu Domu; Wijayanti, Diah Permata; Widowati, Ita; Ginzel, Fanny Iriany; Nursalim, Nining; Kholilah, Nenik; Bachtiar, Muggi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 2 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.2.170-180

Abstract

Threadfin breams (Nemipteridae) are demersal fish species that constitute a significant catch for East Nusa Tenggara fishermen at the Oeba Fish Landing Site and Oesapa Fish Market, where they are landed year-round. Over the years, there has been a noticeable increase in the capture of threadfin breams, raising concerns about the potential impact on their genetic diversity. The ongoing trend could affect the region’s overall population structure of threadfin breams. This study addresses the need to identify threadfin breams in the landing above sites of threadfin breams through molecular analysis of mtDNA COI. The research involved the examination of 24 samples obtained from the Oeba Fish Landing Site and Oesapa Fish Market. The analysis revealed the presence of five distinct threadfin bream species: Nemipterus hexodon, N. japonicus, N. zysron, N. aurora, and Pristipomoides typus. The genetic distance between individual threadfin breams ranged from 0-0.8%, indicating a relatively close genetic relationship within the population. Also, phylogenetic tree reconstruction further delineated five distinct clades based on the species obtained from the samples. Given these findings, the study emphasizes the importance of sustainable threadfin bream capture to preserve genetic diversity. The results underscore the need for ongoing monitoring and management strategies to ensure the threadfin bream population’s long-term health and stability. Additionally, the study suggests that a more in-depth analysis of genetic diversity and the environmental factors influencing this species is warranted for a comprehensive understanding and effective conservation measures.
Biology Fisheries of Threadfin Bream Fish (Family: Nemipteridae) Landed at Oeba Fish Landing Site and Oesapa Fish Market, Kupang City Wora, Umbu Domu; Wijayati, Diah Permata; Widowati, Ita; Nursalim, Nining; Kolilah, Nenik; Ginzel, Fanny Iriyany; Kurniasih, Eka Maya
Jurnal Kelautan Tropis Vol 27, No 2 (2024): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v27i2.22949

Abstract

The threadfin bream fish from the Nemipteridae family is a demersal species and an economically significant commercial fish. It is widely used for personal consumption and sold in markets. The capture of threadfin bream fish at the Oeba Fish Landing Site and Oesapa Fish Market has been increasing. Continuous high-intensity fishing will likely negatively impact the threadfin bream fish population, leading to stock depletion and reduced catch production. Research on threadfin bream fish in East Nusa Tenggara has yet to be reported, particularly in Kupang Bay and Rote Ndao waters. This study aims to investigate the fisheries biology of threadfin bream fish landed at the Oeba Fish Landing Site and Oesapa Fish Market. A total of 84 threadfin bream fish were sampled from both locations. The length-weight relationship of threadfin bream fish at these sites is negatively allometric with value b < 3, indicating that length growth is more dominant than weight gain. The gonad maturity stages of male and female threadfin bream fish range from GSM I-III. The size distribution of threadfin bream fish ranges from 173-295 mm at the Oeba Fish Landing Site and from 173-245 mm at the Oesapa Fish Market. Most of the threadfin bream found are still in the early stages of gonadal maturity, indicating that these fish are young and not yet ready to spawn. Implementing Sustainable fishing management practices is crucial, including setting catch quotas and minimum catch sizes and educating fishermen to ensure the sustainability of threadfin bream fish stocks.
Genetic Analysis on Horseshoe Crab for Phylogenetic Tree Study from Jambi, Bangka Belitung, Central Java, and East Java Province, Indonesia Kholilah, Nenik; Kurniatami, Gabriella Tarida; Kurniasih, Eka Maya; Nursalim, Nining; Janarkho, Galank Fad’qul; Ambariyanto, Ambariyanto; Wijayanti, Diah Permata; Putro, Sapto Purnomo; Cahyani, Ni Kadek Dita
Jurnal Kelautan Tropis Vol 28, No 2 (2025): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v28i2.25923

Abstract

Order Xiphosura, or horseshoe crabs, are rarely found and classified as macrofossils. In Indonesian waters, there are three species: Tachypleus tridentatus, Tachypleus gigas, and Carcinoscorpius rotundicauda. The genetic analysis of their kinship, however, has been extensively unexplored. This study aims to utilize DNA barcoding, focusing on the cytochrome c oxidase subunit I (COI) locus from mitochondrial DNA and investigating the phylogenetic relationships of horseshoe crabs from the four sites (Tanjung Jabung Timur Regency in Jambi, Bangka Regency in Bangka Belitung, Demak Regency in Central Java, and Tuban Regency in East Java). Additionally, the study provides insights into the biodiversity and ecological roles of horseshoe crabs within their ecosystems and surrounding habitats. Through molecular methods, this research focuses on biodiversity analysis through Sanger sequencing and MEGA 11 software for constructing phylogenetic trees and calculating genetic distances. With a total of 22 horseshoe crabs, the DNA samples from four different sites were amplified via Sanger sequencing, targeting the COI locus and analyzing with MEGA 11. The phylogenetic tree analysis revealed two distinct species, Tachypleus gigas, and Carcinoscorpius rotundicauda, exhibiting significant genetic variation between them. A clear genetic separation between the two species was observed. Otherwise, within the C. rotundicauda species, a minor genetic variation was detected between sampling sites. Notably, the genetic composition displayed greater differences between samples from Java and Sumatra compared to differences within each island. The future research should expand the sampling size and include additional genetic markers to provide a more comprehensive understanding of the genetic diversity and evolutionary history of horseshoe crabs in Indonesian waters. Integrating ecological and environmental data could further elucidate the factors driving genetic differentiation and inform conservation strategies.
Co-Authors Ambariyanto , Bachtiar, Muggi Diah Permata Wijayanti Eka Maya Kurniasih, Eka Maya Ginzel, Fanny Iriany Ginzel, Fanny Iriyany Irfandi Irfandi, Irfandi Ita Widowati Janarkho, Galank Fad’qul Kholilah, Nenik Kolilah, Nenik Kurniatami, Gabriella Tarida Marita Ika Joesidawati Negara, Bertoka Fajar Ni Kadek Dita Cahyani, Ni Kadek Dita Nurlaila Ervina Herliany Sapto P Putro Wijayati, Diah Permata Wora, Umbu Domu