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BIOACCUMULATION OF CADMIUM (CD) BY WHITE SHRIMP PENAEUS MERGUIENSIS AT DIFFERENT SALINITY IN KEDUNGMALANG ESTUARY, JEPARA (CENTRAL JAVA) Manullang, Corry Yanti; Hutabarat, Johannes; Widowati, Ita
Marine Research in Indonesia Vol 39, No 1 (2014)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (949.472 KB) | DOI: 10.14203/mri.v39i1.84

Abstract

Estuarine of Kedungmalang has salinity variability and is assumed to bepolluted by cadmium (Cd) derived from human activities around Kedung region. In this study, bioaccumulation of Cd by white shrimp Penaeus merguienis in relation with salinity difference was determined. Sampling was conducted at four stations : estuary (15‰ and 25‰ of salinity) and husbandry area (15‰ and 25‰ of salinity). Data on in situ water quality parameters was obtained at each station. Absorption Atomic Spectrometer was used to measure Cd concentration. The water quality of parameters and the concentrations of Cd in the shrimp were compared with quality standards and weekly consumption limit of white shrimps is also suggested. The Cd concentrations on the white shrimp was found the highest concentration of 0.669 μg.g-1 . The limit comsumption of the white shrimp of Estuarine of Kedungmalang,suggested by the resut of this study, is about 523-1537 grams per week.
BIOACCUMULATION OF CADMIUM (CD) BY WHITE SHRIMP PENAEUS MERGUIENSIS AT DIFFERENT SALINITY IN KEDUNGMALANG ESTUARY, JEPARA (CENTRAL JAVA) Manullang, Corry Yanti; Hutabarat, Johannes; Widowati, Ita
Marine Research in Indonesia Vol 39 No 1 (2014)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (949.472 KB) | DOI: 10.14203/mri.v39i1.84

Abstract

Estuarine of Kedungmalang has salinity variability and is assumed to bepolluted by cadmium (Cd) derived from human activities around Kedung region. In this study, bioaccumulation of Cd by white shrimp Penaeus merguienis in relation with salinity difference was determined. Sampling was conducted at four stations : estuary (15� and 25� of salinity) and husbandry area (15� and 25� of salinity). Data on in situ water quality parameters was obtained at each station. Absorption Atomic Spectrometer was used to measure Cd concentration. The water quality of parameters and the concentrations of Cd in the shrimp were compared with quality standards and weekly consumption limit of white shrimps is also suggested. The Cd concentrations on the white shrimp was found the highest concentration of 0.669 μg.g-1 . The limit comsumption of the white shrimp of Estuarine of Kedungmalang,suggested by the resut of this study, is about 523-1537 grams per week.
Kandungan Seng (Zn) Dalam Air, Sedimen Dan Kerang Darah (Anadara granosa L) Di Perairan Tambaklorok Semarang Kalangie, Dimas Judah Mozes; Widowati, Ita; Suprijanto, Jusup
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.393 KB) | DOI: 10.14710/jmr.v7i1.25887

Abstract

ABSTRAK : Tingginya aktivitas pesisir di daerah Tambaklorok serta adanya penurunan tanah dan abrasi pantai diduga menjadi sumber pencemaran logam berat. Salah satu bentuk pencemaran logam berat tersebut adalah logam berat seng (Zn). Penelitian ini bertujuan untuk menganalisis kandungan logam berat Zn dalam air, sedimen dan jaringan lunak kerang darah (Anadara granosa L) dan mengetahui nilai MTI dan MWI pada manusia. Penelitian ini dilakukan pada bulan November 2015 (uji pendahuluan), pengambilan sampel I (Desember 2015) dan II (Februari 2016). Uji pendahuluan dilakukan dengan cara membeli sampel kerang di pasar Tambaklorok yang langsung diambil dari perairan Tambaklorok. Metode dalam penelitian ini adalah metode deskriptif. Penentuan lokasi sampling dilakukan dengan metode purposive sampling. Analisa logam berat menggunakan AAS. Hasil penelitian menunjukan kandungan logam berat Zn dalam air sebesar 0,005 mg/l dan <0,001 mg/l, sedimen 109,2 mg/kg dan 75,89 mg/kg, Anadara granosa L sebesar 8,59 mg/kg (uji pendahuluan), 19,67 mg/kg, dan 18,68 mg/kg. Hasil menunjukan bahwa air, sedimen dan jaringan lunak kerang darah (Anadara granosa L) di Perairan Tambaklorok masih sesuai dengan baku mutu KMLH No. 51 tahun 2004 dan Wiconsin Department of Natural Resources tahun 2003 untuk sedimen. Nilai MWI dengan asumsi 60 kg untuk laki-laki sebesar 420 mg dan 45 kg untuk perempuan sebesar 315 mg. Nilai MTI asumsi 60 kg laki-laki pada uji pendahuluan sebesar 48,89 kg, Desember 2015 sebesar 21,35 kg dan Februari 2016 sebesar 22,48 kg. Sedangkan asumsi 45 kg untuk perempuan pada uji pendahuluan sebesar 36,67 kg, Desember 2015 sebesar 16,01 kg dan Februari 2016 sebesar 16,86 kg.  ABSTRACT : The high activity in the coastal areas Tambaklorok and a decrease in soil and coastal erosion is suspected to be the source of heavy metal pollution. One form of the heavy metal pollution was heavy metals zinc (Zn). This study aimed to analyze the Zn content of heavy metals in water, sediments and shellfish soft tissue blood (Anadara granosa L) and know the value of MTI and MWI in humans. This research was conducted in November 2015 (preliminary test), sampling I (December 2015) and II (February 2016). Preliminary test done by buying in the market Tambaklorok shellfish samples taken directly from the waters Tambaklorok. The method in this research is descriptive method. Determining the location of the sampling is done by purposive sampling method. Heavy metal analysis using AAS. The results showed Zn content of heavy metals in water of 0.005 mg / l and <0,001 mg / l, sediment 109.2 mg / kg and 75.89 mg / kg, Anadara granosa L of 8.59 mg / kg (preliminary test) , 19.67 mg / kg and 18.68 mg / kg. Results showed that water, sediments and shellfish soft tissue blood (Anadara granosa L) in the waters of Tambaklorok still in accordance with the quality standards KMLH No. 51 in 2004 and Wiconsin Department of Natural Resources in 2003 for sediment. MWI value assuming 60 kg for males at 420 mg and 45 kg for females at 315 mg. MTI value assuming 60 kg male in a preliminary test of 48.89 kg, in December 2015 amounted to 21.35 kg and February 2016 amounted to 22.48 kg. While the assumption of 45 kg for women in a preliminary test of 36.67 kg, in December 2015 amounted to 16.01 kg and February 2016 amounted to 16.86 kg. 
Optimasi Sumber Karbon Dan Nitrogen Sebagai Co-Substrat Untuk Pertumbuhan Bakteri Probiotik Pseudomonas sp. Doresti, Livvy; Setyati, Willis Ari; Widowati, Ita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.394 KB) | DOI: 10.14710/jmr.v7i3.25907

Abstract

ABSTRAK : Pencemaran limbah organik pada sistem tambak udang merupakan salah satu faktor yang menyebabkan terjadinya penurunan produksi. Limbah organik seperti sisa pakan dan feses terakumulasi pada substrat perairan sehingga kualitas air menurun dan timbul penyakit. Pseudomonas sp. merupakan bakteri probiotik yang dapat menghasilkan  enzim  pendegradasi  protein  dan amilum, limbah organik yang banyak ditemukan di sistem tambak. Penelitian ini dilakukan penentuan jenis dan optimasi sumber karbon dan nitrogen untuk pertumbuhan Pseudomonas sp. dalam upaya mengetahui komposisi medium yang lebih baik untuk produksi biomassa bakteri probiotik. Eksperimen yang dilakukan berupa penentuan sumber karbon yang meliputi glukosa, fruktosa dan molase. Penentuan sumber nitrogen meliputi ammonium nitrat, ammonium klorida dan urea. Pengamatan pertumbuhan dilakukan dengan metode spektrofotometri berdasarkan perubahan optical density pada kultur bakteri. Pertumbuhan Pseudomonas sp. memilik fase lag (adaptasi) pada jam ke-0 hingga jam ke-6. Hasil penelitian penentuan jenis sumber karbon diperoleh glukosa dengan nilai OD laju pertumbuhan tertinggi yaitu 0.118±0.001 dan penentuan jenis sumber nitrogen diperoleh ammonium nitrat dengan nilai OD laju pertumbuhan 0.117±0.000. Sumber karbon glukosa optimum pada konsentrasi 4% dengan nilai OD laju pertumbuhan 0.106±0.000 dan sumber nitrogen ammonium nitrat optimum pada konsentrasi 0.5% dengan nilai OD laju pertumbuhan 0.112±0.002. ABSTRACT : The contamination of organic waste in the shrimp pond system is one of the factors that lowering shrimp production. Organic waste such as leftover feed and fecal matter accumulated in the waters substrate decreasing the water quality and emerging disease. Pseudomonas sp. is a probiotic bacteria which can produce enzymes that degrade proteins and amylum-an organic waste commonly found in pond systems. This study was conducted to determine the type and optimization of carbon and nitrogen source for the growth of Pseudomonas sp. in an effort to know the composition of a better medium for probiotic biomass production. Experiments were carried out in the form of determination of carbon sources include glucose, fructose and molasses. Determination of nitrogen sources include ammonium nitrate, ammonium chloride and urea. The observations of growth rate was done by spectrophotometric method based on optical density in a bacterial culture. The growth of Pseudomonas sp. reached lag phase (adaptation) on 0 up to 6 hours. Then, on 6 up to 36 hours the bacterium has exponential phase which is characterized by the significant growth. The results of the determination of carbon source obtained glucose was the highest growth rate 0118 ± 0001 and determination of the nitrogen source obtained ammonium nitrate with growth rate 0117 ± 0000 by the OD value. Optimization carbon source was at 4% concentration of glucose with the growth rate 0.106 ± 0.000, and also the optimization nitrogen source was at 0.5% concentration of ammonium nitrate with growth rate of 0112 ± 0002 by the OD value.
Studi Kandungan Logam Berat Pb, Cu, Cd, Cr Pada Kerang Simping (Amusium pleuronectes), Air Dan Sedimen Di Perairan Wedung, Demak Serta Analisis Maximum Tolerable Intake Pada Manusia Azhar, Husnan; Widowati, Ita; Suprijanto, Jusup
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.451 KB) | DOI: 10.14710/jmr.v1i2.2017

Abstract

Wedung, Demak which have large potential of asian moon scallop (Amusium pleuronectes). This potential has leaded the increasing activities of fishing, industrial, and dense residential in Wedung. Those activities were thought to be one of the sources of heavy metals (Pb, Cu, Cd, Cr) accumulated in the simping. This study was aimed to determine the concentration of Pb, Cu, Cd, Cr in the A. pleuronectes, in the water and the sediment. Moreover, this study was also aimed to investigate the maximum weekly consumption A. pleuronectes which contained Pb, Cu, Cd, and Cr in Wedung waters area, Demak. The study was conducted in October-November 2011. The method used in this study was a study case method, while the purposive sampling method was used to determine the sampling locations. Sampling was conducted once every two week, with three repetitions. AAS (Atomic Absorbtion spectrophotometry), were used to analyze the heavy metal concentration, while the consumption of security analysis was used to analyze the MTI (Maximum Tolerable Intake). The range of the analysis results of Pb concentration in the scallop’s shells were 33,1362 – 35,0762 ppm, and 11,1272 – 13,0852 ppm in the sediments, while the concentration in the soft tissues and water, were not detected. The Cu concentration in the soft tissue were ranged from 8,9849 – 9,4659 ppm, where in the scallop’s shells were 11,9836 – 12,2358 ppm, water were 0,07 – 0,14 ppm, and in the sediments were 13,0624 – 17,6040 ppm. The Cd concentration in soft tissue were ranged from 5,9212 – 8,0136 ppm, scallop’s shell were 2,6195 – 5,0125 ppm, and water were 0,01 ppm at 2nd and 3rd samplings, while at the first sampling was not detected, whereas in the sediments the concentration ranged from 0,4694 – 0,7257 ppm. Cr concentration in the soft tissue were only detected in the third sampling with 0,7285 ppm, while in the scallop’s shells ranged from 1,9694 – 2,6924 ppm, and in the sediments were 0,9693 – 1,6933 ppm, while its concentration in water was not detected. Maximum weekly intake of scallop in Wedung water which are safe for consumption were 22,64 kg for Cu; 0,06 kg for Cd; and 5,76 kg of Cr per week for people with average body weight 60 kg, while the Pb concentration not detected.
Studi Bioekologi Kerang Simping (Amusium pleuronectes) di Perairan Semarang dan Kendal Nursalim, Haviz Rachman; Suprijanto, Jusup; Widowati, Ita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.088 KB) | DOI: 10.14710/jmr.v1i1.1997

Abstract

Asian Moon Scallop (Amusium pleuronectes) is one of scallop species which catch as the secondary crops of the fishing activity. This condition need to understand his biological background, such as the relationship between length and weight, growth, condition index and gonad index. The research purpose is to identify the relationship between length and weight, growth, condition index and gonad index of the Asian Moon Scallop, collected from the Semarang and Kendal Waters. This research was carried out from June to July, 2011 in the Semarang and Kendal waters. Three times collections was done during the research period. A total of 594 individuals of Asian Moon Scallop were collected, which determined there shell length, height, thickness and the total weight. Measurement and observation processes were held in Biology Laboratory, Faculty of Fishery and Marine Diponegoro University, Semarang. The descriptive-explorative methods was used as the research method. While the purposive sampling was applied as the data collection method, and determined the research site. The Asian Moon Scallop growth was determined using the nisbi mothod. The relationship between length and weight, condition index and gonad index were also determined. The b constanta show the value of 3.070, 2.853 and 2.862, which show that the length and weight relationship of Asian Moon Scallop is isometric and alometric negative. The Asian Moon Scallop growth with nisbi method show an equation of 1 cm for the shell length, 0.5 cm for the height, 0.2 sm for the thickness and 5 gr for the total weight of shells. The condition index value of Asian Moon Scallop (A. pleuronectes) is approximately for about 36.01 % - 61.44 %, while the gonad index value is about 8.31 % - 12.09 %.
AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL RUMPUT LAUT COKLAT (Turbinaria conoides dan Sargassum cristaefolium) YANG DIKOLEKSI DARI PANTAI RANCABUAYA GARUT JAWA BARAT Rohimat, Rohimat; Widowati, Ita; Trianto, Agus
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.911 KB) | DOI: 10.14710/jmr.v3i3.6002

Abstract

Rumput laut coklat memiliki potensi sebagai antioksidan alami.  Penelitian ini menggunakan Turbinaria conoides dan Sargassum cristaefolium yang diekstraksi menggunakan metanol untuk mengetahui golongan pigmen dan aktivtias peredaman radikal bebas DPPH (1,1-diphenyl-2- picrylhidrazyl).  Golongan pigmen dianalisis menggunakan spektrofotometer pada panjang gelombang 200-800 nm,  aktivitas peredaman radikal bebas DPPH mengikuti metode Blois (1958) yang digunakan oleh Vijayabaskar dan Shiyamala (2012). Hasil analisis spektrofotometer ekstrak T. conoides dan S. cristaefolium  diperoleh panjang gelombang puncak 416 dan 411 nm yang diidentifikasi sebagai karotenoid, 618 dan 619 nm adalah phycocyanin, serta 665 dan 661 nm yang diidentifikasi sebagai klorofil.  Ekstrak T. conoides menunjukkan aktivitas antioksidan tertinggi dengan nilai  IC50 = 220 ppm dan S. cristaefolium memiliki nilai IC50 = 1603 ppm namun keduanya masih tergolong agen antioksidan lemah (IC50 > 200 ppm).
Kajian Rumput Laut Sargassum duplicatum J. G. Agardh sebagai Penghasil Bioetanol dengan Proses Hidrolisis Asam dan Fermentasi Saputra, Dion Ragil; Ridlo, Ali; Widowati, Ita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.51 KB) | DOI: 10.14710/jmr.v1i2.2031

Abstract

Bioethanol is an alternative fuel made from biomass containing sugar components, starch, and cellulose. So far, bioethanol is made from raw materials which become a source of food and feed resulting in the competition. The potential of cellulosic biomass seaweed S. duplicatum as bioethanol production can be a solution to resolve the issue. The purpose of this research was to study seaweed S. duplicatum J.G. Agardh as raw material for bioethanol. Method used for this research was experimental laboratory with a completely randomized design (RAL) factorial pattern to test glucose levels and a randomized block design (RAK) to test the ethanol levels. Ethanol concentration was determined by Gas Chromatography (GC). Hydrolysis with H2SO4 concentration of 0.2 M, 0.3 M, 0.4 M, 0.5 M and the variation within 30 minutes, 60 minutes, 120 minutes. The fermentation conducted with yeast S. cereviceae with incubation time 24 hours, 48 hours, 72 hours. Ethanol concentration measurements conducted using Gas Chromatography (GC). The results showed that the best conditions for hydrolysis of concentrated H2SO4 0.4 M (28.051 mg / ml ± 1.100) and 120 minutes (23.128 mg / ml ± 6.069). During the fermentation process, the maximum ethanol levels achieved at 72 hours incubation time is 0.0451% v / v ± 0.0098
AKTIVITAS ANTIBAKTERI EKSTRAK RUMPUT LAUT Sargassum cinereum (J.G. Agardh) DARI PERAIRAN PULAU PANJANG JEPARA TERHADAP BAKTERI Escherichia coli DAN Staphylococcus epidermidis Alamsyah, Heru Kurniawan; Widowati, Ita; Sabdono, Agus
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.526 KB) | DOI: 10.14710/jmr.v3i2.4966

Abstract

Munculnya sifat resistensi dan infeksi patogenitas bakteri membuat para ilmuwan berupaya untuk menemukan obat baru. Salah satu upaya yang dilakukan adalah pemanfaatan organisme laut sebagai agen antibakteri alami. Beberapa penelitian melaporkan aktivitas biologis Sargassum sp. dalam bidang farmakologi. Tujuan penelitian ini adalah mengetahui aktivitas antibakteri ekstrak Sargassum cinereum terhadap bakteri E.coli dan S. epidermidis serta mengetahui golongan senyawa dan toksisitas ekstrak S.cinereum. Pengambilan sampel dilakukan secara purposive sampling methods. Pengeringan sampel dilakukan secara kering angin (air drying). Ekstraksi dilakukan secara bertingkat dengan menggunakan tiga pelarut yang berbeda kepolarannya. Uji aktivitas antibakteri dilakukan menggunakan metode difusi agar (Kirby-bauer) konsentrasi 100, 50, 25, 15, 5, 1, 0,5, dan 0,1 µg/disc dengan 3 kali pengulangan. Analisis fitokimia dilakukan secara deskriptif kualitatif berdasarkan perubahan warna serta karakteristik fisika kimia suatu golongan. Uji toksisitas dilakukan menggunakan larva A. salina umur 48 jam (instar III/IV). Nilai toksisitas akut (LC50) dilakukan dengan menggunakan persamaan regresi linier. Hasil penelitian menunjukkan bahwa ekstrak S. cinereum pelarut etil asetat memiliki aktivitas antibakteri terbaik dengan zona hambat 5,08 ± 0,56 (bakteri E. coli) dan 6,69 mm ± 0,14 (bakteri S. epidermidis) serta memiliki aktivitas bakteriosidal. Hasil analisis fitokimia menunjukkan ekstrak Sargassum cinereum mengandung senyawa alkaloid (pelarut etil asetat), steroid (ketiga pelarut), saponin dan tanin (pelarut metanol). Hasil uji toksisitas menunjukkan ekstrak S.cinereum pelarut etil asetat memiliki toksisitas yang sangat toksik dengan nilai LC50-24 jam sebesar 24,25 ppm (sangat toksik kategori kronik).
Biomonitoring Kandungan Logam Berat Timbal (Pb) dalam Air, Sedimen dan Kerang Ceplos (Macridiscus sp.) Serta Analisis Angka Keamanan Konsumsi yang diambil dari Perairan Tambak Lorok, Kota Semarang Tielman, Eduard Meirenno; Suprijanto, Jusup; Widowati, Ita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.72 KB) | DOI: 10.14710/jmr.v7i4.25921

Abstract

ABSTRAK : Logam berat Timbal (Pb) yang diduga berasal dari aktivitas industri di sekitar perairan Tambak Lorok akan mempengaruhi kualitas perairan dan biota yang ada di dalamnya, yaitu Kerang Ceplos (Macridiscus sp.). Tujuan dari penelitian ini adalah untuk menganalisis kandungan logam berat Timbal (Pb) dalam air, sedimen dan Kerang Ceplos (Macridiscus sp.) serta angka keamanan konsumsi. Sampel uji diperoleh dari Perairan Tambak Lorok, Semarang dan diujikan di Balai Besar Teknologi Pencegahan Pencemaran Industri. Metode penelitian adalah metode survey. Pengambilan berdasarkan periode waktu, yaitu bulan September 2015 (pendahuluan dan hanya kerang saja yang diambil), Desember 2015 dan Februari 2016. Analisis logam berat Timbal (Pb) dilakukan dengan menggunakan metode AAS (Atomic Absorption Spectophotometry). Analasis Angka Keamanan Konsumsi menggunakan rumus perhitungan MWI (Maximal Weekly Intake) and MTI (Maximal Tolerable Intake). Hasil penelitian menunjukkan bahwa kandungan logam berat Timbal (Pb) dalam air, sedimen dan Kerang Ceplos (Macridiscus sp.) belum melewati baku mutu. Kandungan logam berat Timbal (Pb) dalam air pada bulan Desember 2015 dan Februari 2016 menunjukan angka < 0,003 mg/l, dalam sedimen sebesar 0.197 mg/Kg dan 0.124 mg/Kg, dalam Kerang Ceplos (Macridiscus sp.) sebesar 0.18 mg/kg dan < 0.1 mg/kg. Angka Keamanan Konsumsi Kerang Ceplos (Macridiscus sp.) yang terpapar logam berat Timbal (Pb) pada Desember 2015 dan Februari 2016 dengan nilai MWI logam berat Pb 1,125 mg/orang (berat badan 45 kg), yaitu 6.25 dan 11.25 kg/minggu/orang kemudian untuk berat badan 60 kg, yaitu 8.34, 15 kg/minggu/orang dengan nilai MWI 1,5 mg/orang. ABSTRACT : Heavy metal Lead (Pb) which suppose sourced from industry activity around Tambak Lorok Water Area, can impact to those waters area, also organisms Kerang Ceplos (Macridiscus sp.). This study was to analyze heavy metal concentration Lead (Pb) in the waters, sediment and Kerang Ceplos (Macridiscus sp.) and safety Number of total Intake of Kerang Ceplos (Macridiscus sp.). Research sample was taken from Tambak Lorok Water Area. Research method was Survey Method. Analysis method of heavy metal Lead (Pb) used AAS (Atomic Absorption Spectophotometry) method. safety number of total intake by using MWI (Maximal Weekly Intake) and MTI (Maximal Tolerable Intake). The result of this research showed that heavy metal concentrate in waters, sedimen and Kerang Ceplos (Macridiscus sp.) was not exceed of quality standard yet. The number of heavy metal concentrataion Lead (Pb) in Kerang Ceplos (Macridiscus sp.) for 2 (two) different months were 0.18 mg/Kg for December 2015 and < 0.1 mg/Kg for February 2016. The numbers of the water were < 0.003 mg/L in December 2015 and February 2016. for the sediment were 0.197 mg/Kg in December 2015 and 0.124 mg/Kg in February 2016. The number of MTI for Kerang Ceplos (Macridiscus sp.) in two differents month were 6.25 and 11.25 kg/week/person for body weight of 45 kg with MWI number of  heavy metal Pb (1.125 mg/Kg). Then 8.34, 15 kg/week/person for body weight of 60 kg with MWI number of  heavy metal Pb (1.5 mg/Kg).
Co-Authors - Desrina, - A Suhaeli Fahmi Abdul Ghofar Adi Santoso Adi Santoso Agus Sabdono Agus Sarwono Agus Trianto Agus Trianto Aldi Rivaldy Maulana Ali Ridlo Ambariyanto Ambariyanto Ambariyanto Ambariyanto Andri Wijayanto Anicia Hurtado Anindya Wirasatriya Antonius Budi Susanto Arif Rahman Arya Fernandes Ayu Safitri Bachtiar, Muggi Bambang Cahyono Bayu Legowo Chrisna Adhi Suryono Christel Marty Diah Permata Wijayanti Dimas Judah Mozes Kalangie Dion Ragil Saputra Dion Ragil Saputra Djoko Suprapto Doresti, Livvy Dwi Lestari Widya Ningsih Eduard Meirenno Tielman Eduard Meirenno Tielman Eka Maya Kurniasih, Eka Maya Elsa Lusia Agus Endang Supriyantini Endang Supriyantini Erian Febri Satriawan Ervia Yudiati Ervia Yudiati Erwin Ivan Riyanto Erwin Ivan Riyanto Fabian Panji Ayodya Fadhel Muhammad Juharna Fitriyan, Jodhi Kusumayudha Ginzel, Fanny Iriany Ginzel, Fanny Iriyany Hadi Endrawati Hakim, Muhamad Fikri Hudi Nur Haviz Rachman Nursalim Haviz Rachman Nursalim Hendi Perdian Yunianto Hendi Perdian Yunianto Herlina Lestari Pakpahan Hermin Pancasakti Kusumaningrum Heru Kurniawan Alamsyah Husnan Azhar Husnan Azhar Ika Wulan Santi Ika Wulan Santi Imai Hideyuki Iqomatuddin, Muhammad Irwan Effendi Irwani Irwani Ita Riniatsih Johannes Hutabarat Jusup Suprijanto Jusup Suprijanto Jusup Suprijanto Jusup Supriyanto Kalangie, Dimas Judah Mozes Karina Dewiningsih Khairunnisa Khairunnisa Kholilah, Nenik Kolilah, Nenik Kristi, Roselina Nadya Liningga Adiningtyas Lisa F. Indriana Lisa F. Indriana Livvy Doresti Manullang, Corry Yanti Manullang, Corry Yanti Maya Puspita Michael Abbey Michael Abbey Mimie Saputri Mimie Saputri, Mimie Muhamad Fikri Hudi Nur Hakim Muhammad Zainuri Muhammad Zainuri Muhammad Zainuri Muji Wasis Indriyawan, Muji Wasis Muliawati Handayani Mustagfirin Mustagfirin Mustagpirin Mustagpirin Nadaa Salsabiila Nuri Nathalie Bourgougnon Nathalie Bourgougnon Novianti, Teni Nugroho, Suciadi Catur Nursalim, Nining Ocky Karna Radjasa Pakpahan, Herlina Lestari Panji Ayodya Petta, Constantein Ramadhani, Muhammad Rizqi Retno Hartati Rexie Magdugo Ria Azizah Tri Nuraini Riza, Subkhan Rohimat Rohimat Rohimat Rohimat Rohmaniyah, Lailatur Rudhi Pribadi Satriawan, Erian Febri Satrio Fahrul Ananda Setyati, Willis Ari Sri Redjeki Sutrisno Anggoro Sutrisno Anggoro Syukur Syukur Teni Novianti Teni Novianti Tielman, Eduard Meirenno Tjahjo Winanto Tri Winarni Agustini Triana Hanani Valerie Stiger-Pouvreau Wahyudi, Yudisthio Wibowo, Muhammad Reyhan Widianingsih Widianingsih Wijayati, Diah Permata Wilis Ari Setyati Willis Ari Setyati Wora, Umbu Domu Yann Hardivillier Yoki Ristadi Yuni Karnisa Zhulian Hikmah Hasibuan