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All Journal JURNAL BIOLOGI INDONESIA Jurnal Biodjati GEMASSIKA: Jurnal Pengabdian Kepada Masyarakat PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal Kedokteran Hewan
Wulandari, Sri Wijayanti
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Biochemistry, Genetics & Molecular Biology Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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PENANDA GENETIK TARSIUS (TARSIUS SPP.) DENGAN MENGGUNAKAN GEN CYTOCHROME OXIDASE I (COI) DNA MITOKONDRIA (MTDNA) MELALUI METODE SEKUENSING Kuswandi, Paramita Cahyaningrum; ,, Wirdateti; Wulandari, Sri Wijayanti
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2202

Abstract

ABSTRACTTarsier (Tarsius spp.) are the smallest primates in the world. Currently there are 10 species, of which 9 species are distributed in Indonesia and 8 species of them are endemic to Sulawesi. Morphologically  the Sulawesi spesies are almost similar. This research is aimed to identify the use of Cytochrome Oxidase I (COI) gene as a genetic marker on Tarsius spp. for conservation purposes. Sixteen individuals consisted of 10 Tarsius bancanus, 4 Tarsius sp., 1 Tarsius wallacei , and 1 Tarsius sangirensis were collected from various places and analysed using COI gene. The results showed  there were 238 different sites of nucleotides and 159 sites of amino acids from the total amount of 838 bp. The genetic distance by Kimura-2 parameter showed the highest value was 26% while the lowest was 0%. The average genetic distance was 11,5%. Phylogenetic tree constructed by Neighbour-Joining method based on nucleotides sequence showed that the COI gene could be used as a genetic marker to differentiate among Tarsius spp. but could not be used as a clear marker for tarsiers in Sulawesi. Based on the analysis, there is a high value of genetic variation among Tarsius spp. with much lower genetic variation in Western Tarsier population compared to Eastern Tarsier. Keyword: Genetic marker, tarsier, COI, DNA mitochondria, conservation 
PEMERIKSAAN KESEHATAN GURU DAN SISWA DI SMK MUHAMMADIYAH NGAWEN GUNUNGKIDUL Indra Putra, Ichsan luqmana; Wulandari, Sri Wijayanti; Utami, Nurul Putrie
GEMASSIKA : Jurnal Pengabdian Kepada Masyarakat Vol 4, No 1 (2020): MEI
Publisher : P3M STIKES Aisyiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30787/gemassika.v4i1.494

Abstract

Gunungkidul merupakan kabupaten yang terdapat di Yogyakarta dan memiliki tingkat kecukupan gizi yang rendah. Banyak kasus tentang kekurangan gizi yang terjadi di beberapa kecamatan di Gunungkidul. Rendahnya angka kekurangan gizi tersebut diperparah dengan tingginya tingkat penderita anemia di Gunungkidul, salah satunya di Kecamatan Ngawen. Kegiatan ini bertujuan untuk memberikan sosialisasi dan pemeriksaan gizi kepada siswa dan guru SMK Muhammadiyah di Kecamatan Ngawen. Kegiatan dilakukan selama 3 hari berturut-turut. Kegiatan diawali dengan sosialisasi tentang kecukupan gizi dan anemia, baik dari segi penjelasan ataupun cara penanggulangan. Kegiatan kemudian dilanjutkan dengan pemeriksaan kesehatan bagi guru dan siswa SMK. Pemeriksaan kesehatan yang dilakukan adalah pemeriksaan TB (tinggi badan), BB (berat badan), HB (hemoglobin), tekanan darah, kolesterol, asam urat dan glukosa. Untuk siswa dilakukan pemeriksaan TB, BB, HB dan tekanan darah, sedangkan untuk guru semua pemeriksaan dilakukan. Hasil yang didapatkan menunjukkan bahwa kondisi kesehatan guru dan murid yang didapatkan masih dalam masuk kisaran normal. Kesimpulan dari penelitian ini adalah kondisi siswa dan guru di SMK Muhammadiyah Ngawen tergolong normal dan perlu adanya pengontrolan kondisi kesehatan guru dan murid. 
Effect of Calina Papaya Leaves Extract on Respiratory Tract in Cigarette Smoke Exposed Rats Setiawan, Haris; Hikmawati, Arlinda Sekar Ayu; Wulandari, Sri Wijayanti; Malinda, Eriza Via; Putra, Ichsan Luqmana Indra
Jurnal Biodjati Vol 7 No 2 (2022): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v7i2.19068

Abstract

Cigarette smoke is a source of free radicals that cause health problems throughout the world. Indonesia is a country that has many sources of natural antioxidants in counteracting free radicals, one of which is Calina papaya leaves. The study aimed to determine the potential of Calina papaya leaves antioxidants in improving the respiratory organs of Wistar rats after being exposed to cigarette smoke. The study using 25 male Wistar rats consisted of Control (not exposed to cigarette smoke and given distilled water), KN (cigarette smoke and given distilled water), P1 (cigarette smoke and Calina Papaya Leaves Ethanolic extracts (CPLE) 100 mg/kg BW), P2 (cigarette smoke and CPLE 200 mg/kg BW), and P3 (cigarette smoke and given CPLE 300 mg/kg BW). Exposure to cigarette smoke and administration of extracts was carried out for 21 days. On day 22, the rats were euthanized and dissected to remove the lungs and trachea. Organs were prepared using the paraffin method and hematoxylin-eosin staining. Parameters consisted of histopathological observations of the lungs and trachea. All data parameters were analyzed using a one-way ANOVA test and Duncan's advanced test (p<0.05). The results showed that there was an improvement in histopathology of the lungs and trachea at a dose of 200 mg/Kg BW compared to other doses (p<0.05). The study showed that the Calina papaya leaves ethanolic extracts have the potential as a source of antioxidants in improving the respiratory organs of Wistar rats after being exposed to cigarette smoke.
PENAPISAN ANTIBODI TOXOPLASMA GONDII DAN PROFIL LEUKOSIT PADA DARAH DONOR Wulandari, Meyta; Nafilata, Ikrimah; Wulandari, Sri Wijayanti
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 8 No. 2 (2024): AGUSTUS 2024
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v8i2.31624

Abstract

Keamanan produk darah merupakan prioritas bagi UTD dalam menyediakan komponen darah donor sebagai obat esensial yang memiliki risiko menularkan penyakit. Penapisan darah donor telah diatur dalam Peraturan Menteri Kesehatan No 91 tahun 2015 untuk uji empat parameter penyakit, diantaranya sifilis, HIV, hepatitis B dan hepatitis C. Toksoplasmosis merupakan infeksi protozoa yang dapat bersifat asimptomatik pada penderitanya. Prevalensi toksoplasma di Asia, khususnya di Indonesia cukup tinggi, sehingga perlu menjadi perhatian bagi UTD melakukan pengamanan darah donor. Infeksi toksoplasma dapat menyebabkan penyakit komorbit pada individu imunodefisiensi. Penularan toksoplasmosis terhadap penderita HIV dengan CD4 yang rendah dapat menyebabkan tambahan penyakit opurtunistik yaitu toksoplasma ensefalitis serta infeksi laten yang menunjukkan risiko komplikasi. Tujuan penelitian ini adalah mendeteksi antibodi IgG/IgM Toxoplasma gondii pada darah donor dengan desain penelitian deskriptif laboratorik. Sampel didapatkan dari 69 darah donor berupa serum. Deteksi antibodi yang dilakukan menggunakan Rapid Test Voyage Toxo IgG/IgM. Hasil menunjukkan bahwa terdapat 19% sampel reaktif toksoplasma antibodi imunoglobulin G (IgG), 81% sampel non reaktif IgG, tidak ditemukan sampel darah donor yang reaktif IgM (0%) dan profil leukosit pada sampel darah donor reaktif IgG diketahui leukosit jenis limfosit dan monosit, keduanya meningkat 85% dari nilai normal. Disimpulkan pendonor memiliki riwayat terinfeksi Toxoplasma gondii dan berpeluang dalam menularkan parasit melalui transfusi darah secara horizontal.
ANTISPERMATOGENIC ACTIVITY OF ETHANOLIC EXTRACT OF CALINA PAPAYA LEAVES ON SEMINIFEROUS TUBULES WISTAR RATS Setiawan, Haris; Wulandari, Sri Wijayanti; Agustina, Erlita Devi
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.18435

Abstract

Indonesia is a country with high birth rate and the rate continues to increase each year. Methods to reduce high birth rate are needed, especially herbal based contraceptives. This research aims to study the possibility of Calina papaya leaf ethanolic extract as an antispermatogenic agent which hopefully could be expanded into natural antifertiliy drug candidate. Research was done using 24 white male Wistar rats divided into four groups. Dosages were given as follows: 0 mg/Kg W (control group), 100 mg/Kg W (P1), 200 mg/Kg W (P2) and 300 mg/Kg W (P3). Treatment was given in 30 days period. Rats were sacrificed on Day 31 and the testes were taken. Testes were then made into histological specimen using paraffin block and stained using Haematoxylin-Eosin. The diameter and surface area of seminiferous tubules, lumen surface area, and spermatogenesis index were then observed. Results showed that there is a significant difference (p0.05) on lumen surface area and spermatogenesis index between control and treatment groups. It is also found that the optimum dosage given to attain the desirable effect is 300 mg/Kg W. There is no significant difference found on surface area and diameter of tubulus seminiferous between control and treatment group (p0.05). Based on the results, papaya leaf (Calina variety) ethanolic extract has the potential a natural antispermatogenic with the optimum dose of 300 mg/Kg W.
Co-Authors ,, Wirdateti Agustina, Erlita Devi Aris Setiawan Hikmawati, Arlinda Sekar Ayu Ichsan Luqmana Indra Putra, Ichsan Luqmana Indra Malinda, Eriza Via Meyta Wulandari Nafilata, Ikrimah Nurwijayanti Paramita Cahyaningrum Kuswandi Utami, Nurul Putrie