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All Journal Jurnal Penelitian Saintek Jurnal Biologi Udayana Jurnal Sains Dasar AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Scientiae Educatia: Jurnal Pendidikan Sains JURNAL BIOLOGI INDONESIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA AL-HAYAT: Journal of Biology and Applied Biology Jurnal Mangifera Edu Bioscientist : Jurnal Ilmiah Biologi Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Kingdom (The Journal of Biological Studies) IJOBI Biosaintifika: Journal of Biology & Biology Education

Paramita Cahyaningrum Kuswandi

Jurusan Pendidikan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Yogyakarta

Author-ID : 2977321

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Neuroscience Physics Public Health Social Sciences

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Induksi Keragaman Somaklonal Bunga Kertas (Zinnia sp.) Sebagai Upaya Pengembangan Bunga Potong Daerah Tropis Kuswandi, Paramita Cahyaningrum; Sugiyarto, Lili
Jurnal Sains Dasar Vol 2, No 1 (2013): April 2013
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Abstrak Keragaman bunga kertas saat ini berasal dari persilangan atau mutasi yang dilakukan oleh manusia. Penelitian ini bertujuan untuk menginduksi keragaman bunga kertas dengan metode kultur jaringan. Penelitian dilakukan di Laboratorium Kultur Jaringan dan Kebun Percobaan FMIPA, UNY. Metode yang digunakan dalam penelitian ini adalah induksi menggunakan sitokinin BAP (benzyl amino purin) dalam media MS. Eksplan diperoleh dari tanaman bunga kertas yang sudah dikecambahkan di tray perkecambahan dan penanaman in vitro. Sterilisasi untuk penanaman dengan teknik kultur jaringan menggunakan detergen, alkohol 70%, Clorox 10% dan 5%, serta aquadest steril. Media yang digunakan adalah media MS (Murashige dan Skoog) ditambah dengan BAP (konsentrasi 2,4,6, dan 15ppm). Hasil penelitian menunjukkan penambahan BAP dalam media MS mampu memicu pertumbuhan kalus pada eksplan nodia dan daun Zinnia sp. Konsentrasi 4 dan 6 ppm BAP mampu menginduksi tunas dari kalus pada eksplan nodia dan keragaman hanya dapat dilihat pada tipe kalus. Terdapat perbedaan tipe kalus jika konsentrasi BAP sangat tinggi (15 ppm). Pengamatan pada sel-sel ujung akar hanya menunjukkan sel-sel berukuran kecil dengan inti sel tanpa terlihat kromosom di dalamnya. Perlu penelitian lebih lanjut untuk induksi tunas atau embrio somatik dari kalus hasil induksi BAP pada media. Pengamatan kromosom dapat diperbaiki dengan penambahan zat kimia lain atau perubahan waktu pengambilan sampel dan perendaman. Kata Kunci : keragaman, Zinnia, BAP, kromosom

APPLICATON OF MYCORRIZA ON PLANTING MEDIA OF TWO TOMATO VARIETIES TO INCREASEVEGETABLE PRODUCTIVITY IN DROUGHT CONDITION Kuswandi, Paramita Cahyaningrum; Sugiyarto, Lili
Jurnal Sains Dasar Vol 4, No 1 (2015): April 2015
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

A method used for the development of dry areas/marginal lands is the improvement of soil structure and addition to the media to support the growth and development of crops. Tomatoes have the potential to be developed in marginal lands due to its high nutrition, high in demand and easy to be cultivated. One of the method used to improve planting media is the addition of microorganism such as mycorrhiza which can help the absorbtion of water and nutrition for plants. The interval of irrigation is used as a simulation of drought. This research aim was to observe the effect of mycorrhiza in the soil on the growth and development of tomato with several treatments of irrigation. The method used was the addition of 4 g of mycorrhiza per polybag (size 30x30 cm2), using Complete Randomized Design. There were 6 combinations of treatments. The treatments were : 3 interval of irrigation (every day, every 7 days and every 14 days), and 2 treatments of mycorrhiza (0 g and 4 g). There were 3 repetition for each combination of treatments. The results showed that the addition of mycorrhiza can increase significantly plant fresh and dry weight and also root length. The difference in plant height, number of branches, number of leaves, plant growth rate and percentage of infection were caused by the difference in irrigation interval. The difference in the varieties used also contribute to a difference in the percentage of infection. Further research must be made on the effect of mycorrhiza with addition of inorganic fertilizer to increase the growth and development of tomato plants in water stressed condition. Keywords: mycorriza, tomato, draught simulation

Penanda Genetik Tarsius (Tarsius spp.) dengan Menggunakan Gen Cytochrome Oxidase I (COI) DNA Mitokondria (mtDNA) Melalui Metode Sekuensing ,, Wirdateti; Wulandari, Sri Wijayanti; Kuswandi, Paramita Cahyaningrum
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTTarsier (Tarsius spp.) are the smallest primates in the world. Currently there are 10 species, of which 9 species are distributed in Indonesia and 8 species of them are endemic to Sulawesi. MorphologicallyÂ the Sulawesi spesies are almost similar. This research is aimed to identify the use of Cytochrome Oxidase I (COI) gene as a genetic marker on Tarsius spp. for conservation purposes. Sixteen individuals consisted of 10 Tarsius bancanus, 4 Tarsius sp., 1 Tarsius wallacei , and 1 Tarsius sangirensis were collected from various places and analysed using COI gene. The results showedÂ there were 238 different sites of nucleotides and 159 sites of amino acids from the total amount of 838 bp. The genetic distance by Kimura-2 parameter showed the highest value was 26% while the lowest was 0%. The average genetic distance was 11,5%. Phylogenetic tree constructed by Neighbour-Joining method based on nucleotides sequence showed that the COI gene could be used as a genetic marker to differentiate among Tarsius spp. but could not be used as a clear marker for tarsiers in Sulawesi. Based on the analysis, there is a high value of genetic variation among Tarsius spp. with much lower genetic variation in Western Tarsier population compared to Eastern Tarsier.Â Keyword: Genetic marker, tarsier, COI, DNA mitochondria, conservationÂ

PENANDA GENETIK TARSIUS (TARSIUS SPP.) DENGAN MENGGUNAKAN GEN CYTOCHROME OXIDASE I (COI) DNA MITOKONDRIA (MTDNA) MELALUI METODE SEKUENSING Kuswandi, Paramita Cahyaningrum; ,, Wirdateti; Wulandari, Sri Wijayanti
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2202

ABSTRACTTarsier (Tarsius spp.) are the smallest primates in the world. Currently there are 10 species, of which 9 species are distributed in Indonesia and 8 species of them are endemic to Sulawesi. Morphologically the Sulawesi spesies are almost similar. This research is aimed to identify the use of Cytochrome Oxidase I (COI) gene as a genetic marker on Tarsius spp. for conservation purposes. Sixteen individuals consisted of 10 Tarsius bancanus, 4 Tarsius sp., 1 Tarsius wallacei , and 1 Tarsius sangirensis were collected from various places and analysed using COI gene. The results showed there were 238 different sites of nucleotides and 159 sites of amino acids from the total amount of 838 bp. The genetic distance by Kimura-2 parameter showed the highest value was 26% while the lowest was 0%. The average genetic distance was 11,5%. Phylogenetic tree constructed by Neighbour-Joining method based on nucleotides sequence showed that the COI gene could be used as a genetic marker to differentiate among Tarsius spp. but could not be used as a clear marker for tarsiers in Sulawesi. Based on the analysis, there is a high value of genetic variation among Tarsius spp. with much lower genetic variation in Western Tarsier population compared to Eastern Tarsier. Keyword: Genetic marker, tarsier, COI, DNA mitochondria, conservation

EFFECT OF 2,4-DICLOROFENOKSIASETAT (2,4-D) AND BENZYL AMINOPURIN (BAP) ON THE GROWTH OF BINAHONG LEAF CENTER (ANREDERA CORDIFOLIA L.) AND ANALYSIS OF FLAVONOID TOTAL CONTENT Lili Sugiyarto; Paramita Cahyaningrum Kuswandi
Jurnal Penelitian Saintek Vol 19, No 1: April 2014
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The aim of this research was to study the effect of various concentration of Plant Growth Regulator in MS (Murashige and Skoog) media on callus growth of binahong leaf and total flavonoid content. The method used in the propagation of callus was the leaf explant of binahong with a Completely Randomized Design (CRD). The leaf explants were planted on MS media with different 2,4-D concentrations (1;2;3 ppm), 0,5ppm IBA+0,5ppm BAP; 0,5ppm IBA+1,0 ppm BAP ; 1,0 ppm IBA+0,5 ppm BAP, each with 15 repetition. The parameters observed in this research were initiation time, type, colour, diameter, the number of callus and total flavonoid content. The result showed that the optimum growth of callus is at 3 weeks and after that it declined or stayed stagnant. The result of the analysis of variance (ANOVA) showed that there is no significant difference in the media used in this research. The total flavonoid content of fresh leaf sample is higher than callus sample.

Callus induction of binahong leaves (Anredera cordifolia L.) for the development of traditional medicinal plant Lili Sugiyarto; Paramita Cahyaningrum Kuswandi
Jurnal Sains Dasar Vol 3, No 1 (2014): April 2014
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

A plant that has a good prospect in the development and cultivation as medicinal plant is binahong (Anredera cordifolia L.). Secondary metabolites have been used as coloring substance, food coloring, and medicine. Callus induction using tissue culture has been used to produce secondary metabolite with a higher concentration than obtained directly from the plant. The aim of this research was to induce callus from the leaf of Binahong using various concentrations of Plant Growth Hormones in MS media. The method used in the propagation of callus used the leaf explant of binahong with Completely Randomized Design (CRD). The leaf explants were planted on MS media with different 2,4-D concentrations (1;2;3 ppm), 0,5ppm IBA+0,5ppm BAP; 0,5ppm IBA+1,0 ppm BAP; and 1,0 ppm IBA+0,5 ppm BAP, each with 15 repetitions. The result showed that the addition of 2,4-D (1 and 2 ppm) was able to induce callus from Binahong leaves on 3 and 5 days after planting, and also 10 days after planting in the controlled and combination treatment. In the 2,4-D media (1 and 2 pm), the callus produced were watery white and compact, while in the 3 ppm 2,4-D media the callus were white and friable. In the media with a combination of BAP and INA the callus were green and compact. The highest average diameter of the callus after 8 weeks in the 1 ppm 2,4-D media reached 2.07 cm followed by the 2 ppm and 3 ppm 2,4-D media producing a diameter of 1.8 cm. In the combination media (BAP and IBA), the callus diameter was less than 0.3 cm. The highest percentage of callus formed was in the media MS+1 ppm 2,4-D and a combination of MS +0,5ppm IBA+0,5ppm BAP that reached 100%. The lowest was found in the control (MS) which reached only 20%. The optimum growth of callus was at 3 weeks and after that it declined or stayed stagnant. The result of the analysis of variance (ANOVA) showed that there was no significant difference in the media used in this research. Key words: induction, binahong leaf, callus, plant growth

Eksplorasi Metode Sterilisasi dan Macam Media Untuk Perbanyakan Durian (Durio zibethinus, L.) Secara In Vitro Lili Sugiyarto; Paramita Cahyaningrum Kuswandi
Jurnal Sains Dasar Vol 2, No 1 (2013): April 2013
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Abstrak Durian (Durio zibethinus, L.) memiliki nilai ekonomis tinggi baik sebagai salah satu komoditas hortikultura yaitu buah untuk konsumsi di pasar lokal maupun untuk ekspor. Salah satu hambatan adalah persediaan bibit yang tidak mencukupi dan metode perbanyakan yang merusak pohon induk dengan karakter unggul. Penelitian ini bertujuan untuk memperoleh metode sterilisasi yang tepat dan media untuk perbanyakan durian secara in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan dan Kebun Percobaan FMIPA, UNY. Metode yang digunakan dalam penelitian ini adalah induksi menggunakan sitokinin BAP (benzyl amino purin) dalam media MS. Eksplan diperoleh dari bibit durian yang sudah dikecambahkan di polibag dengan media tanah dan kompos. Sterilisasi untuk penanaman dengan teknik kultur jaringan menggunakan detergen, clorox 20% dan 10%, alkohol 70%, dan aquades steril untuk nodia, sedangkan sterilisasi eksplan daun dengan clorox 10% dan 5%. Media yang digunakan adalah media MS (Murashige dan Skoog) ditambah dengan BAP (konsentrasi 2 dan 4 ppm), serta 2,4-D (0.4, 1.0, dan 1.5 ppm). Hasil penelitian menunjukkan penambahan BAP dalam media MS mampu memicu pertumbuhan tunas pada eksplan nodia durian pada konsentrasi 2 dan 4 ppm. Penambahan 2,4-D mampu menginduksi kalus pada daun durian. Pada konsentrasi 1 ppm kalus yang dihasilkan berwarna putih, berair dengan daun yang tidak terlalu menggulung sedangkan konsentrasi 0,4 dan 1,5 ppm menghasilkan kalus hijau dengan daun yang menggulung. Perlu perlakuan media yang lebih bervariasi baik pada eksplan nodia maupun daun untuk menghasilkan planlet melalui kalus atau pembentukan tunas secara langsung. Kata kunci: durian, sterilisasi, BAP, 2,4-D, tunas, kalus Abstract One of the problems that can occur in the development of durian (Durio zibethinus, L.) is the availability of seedlings and the method of propagation can cause over-exploitation of the mother plant. The aim of this research was to obtain the method of sterilization and the right media for in vitro propagation of durian. The research was carried out in the Tissue Culture Laboratory and Experimental Garden Faculty of Mathematics and Science, Yogyakarta State University. The method used in this research was induction using the cytokinin BAP (benzylamino purine) in MS media. The explants were obtained from durian seedlings that were grown in polybags with soil and compost media. Sterilization for the tissue culture technique was done using detergent, alcohol 70%, and clorox (20% and 10%), and also sterile aquadest for nodia, while leaf exsplant sterilization was done using clorox 10% and 5%. The media used were MS (Murashige and Skoog) with BAP (2 dan 4 ppm) for the nodia and also with 2,4-D (0.4, 1.0, dan 1.5 ppm) for the leaf explants. The result showed that the BAP in the MS media was able to induce the growth of shoots from the nodia at concentrations of 2 and 4 ppm. The addition of 2,4-D was able to induce the growth of callus on the leaf explants. Using 1 ppm 2,4-D, the callus was white and transparent and the leaves did not curl so much as in the treatments with 0.4 and 1.5 ppm 2,4-D which caused the leaves to curl. More variation in the treatments or media both for the nodia and leaves explants can be further investigated to produce plantlets via callus induction or direct organogenesis. Key words: durian, sterilization, BAP, 2,4-D, shoot, callus

Analisis Korelasi Indeks Massa Tubuh (IMT) dalam Keluarga Remaja Putri yang Mengalami Obesitas di Yogyakarta Paramita Cahyaningrum Kuswandi; Tutiek Rahayu
Jurnal Sains Dasar Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v11i1.44921

Obesitas merupakan salah satu masalah kesehatan bagi masyarakat di dunia. WHO memperkirakan terdapat 2,8 juta orang yang meninggal akibat obesitas. Prevalensi obesitas pada orang dewasa dengan usia 18 tahun di Indonesia, terutama di DIY mengalami peningkatan dari tahun 2007-2018. Salah satu faktor yang dapat menyebabkan obesitas yaitu faktor genetik. Peran faktor genetik dalam pewarisan obesitas akan tampak pada pola pewarisan IMT di dalam keluarga dan dapat dilihat secara kuantitatif dengan adanya korelasi antara IMT anggota keluarga. Penelitian ini bertujuan untuk mengetahui korelasi antara IMT ayah, ibu, kakek, dan nenek dengan IMT remaja putri yang mengalami obesitas di Yogyakarta. Penelitian ini merupakan penelitian observasional dengan desain cross sectional. Teknik sampling yang digunakan yaitu purposive sampling. Responden pada penelitian ini adalah 36 remaja putri usia 15-24 tahun dengan IMT ≥ 25 di Yogyakarta. Variabel independen penelitian adalah IMT ayah, ibu, kakek, dan nenek. Variabel dependen penelitian adalah IMT remaja putri. Data IMT dianalisis menggunakan SPSS versi 15. Uji yang digunakan yaitu uji normalitas Shapiro-Wilk, uji korelasi Spearman, dan uji korelasi berganda. Hasil penelitian menunjukkan bahwa tidak terdapat hubungan yang signifikan (p 0,05) antara IMT keluarga (ayah, ibu, kakek, dan nenek) dengan IMT remaja putri yang mengalami obesitas di Yogyakarta.

Upaya Pencegahan Penyebaran Covid-19 dan Penguatan Kekebalan Tubuh Melalui Pelatihan Pengembangan Imunosuplemen Berbasis Potensi Lokal Kartika Ratna Pertiwi; Paramita Cahyaningrum Kuswandi; Tutiek Rahayu; Rizka Apriyani Putri
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 6, No 1 (2022): Vol 6, No 1(2022)
Publisher : Yogyakarta State University

Kader kesehatan merupakan salah satu wujud kontribusi masyarakat dalam upaya pemeliharaan kesehatan dan pemberantasan penyakit, termasuk Covid-19. Program ini bertujuan memberikan pengetahuan kepada kader kesehatan Gondanglegi, Wedomartani, Yogyakarta tentang upaya pencegahan Covid-19 dengan penguatan sistem imun menggunakan bahan-bahan alam potensi lokal, sekaligus penguatan keterampilan budidaya tanaman rimpang serta pengolahan produknya menjadi imunosuplemen. Metode kegiatan berupa persiapan, pelaksanaan berupa ceramah, diskusi dan simulasi dilanjutkan praktek perwakilan, serta ditutup dengan evaluasi keberhasilan berupa lomba. Hasil kegiatan ini berupa kehadiran peserta (91,7%), pencapaian skor pengetahuan kader (90) serta skor persepsi kader (90). Kreativitas kader dalam penerapan pengetahuan mengenai herbal imunosuplemen terlihat dari produk yang dikembangkan berupa biskuit jahe, puding telang, dan minuman herbal “jaselem” dan “jagujek”. Penilaian produk meliputi komposisi bahan imunomodulator, proses pembuatan dan tampilan produk. Hasilnya didapatkan rerata skor 88,67 (jahe), 85,83 (puding telang), 85,1 (jaselem) serta 87,56 (jagujek). Kendala pembatasan kegiatan dalam kurun pengabdian disiasati dengan pembatasan jumlah pertemuan, jumlah partisipan serta durasi waktu kegiatan. Program ini diharapkan dapat dilanjutkan kader dengan dukungan padukuhan dan puskesmas setempat untuk meningkatkan bukan hanya kesehatan tapi juga perekonomian masyarakat.

Exploring Students’ Perspective on Using Online Collaborative Platform in Biology Laboratory Work Rizqa Devi Anazifa; Suratsih Suratsih; Paramita Cahyaningrum Kuswandi; Anggi Tias Pratama
Scientiae Educatia: Jurnal Pendidikan Sains Vol 12, No 1 (2023): June 2023
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v12i1.11896

The COVID-19 pandemic has changed the teaching and learning methods. Learning activities that were originally done face-to-face must be adjusted to online learning. This change also occurs in laboratory activities. As students must adapt to the different method of learning, their behavior in learning is affected, as well, including how they collaborate in laboratory work that is conducted online. This study aims to explore students’ perspective on online collaborative learning tool in biology laboratory work. The online collaborative learning process is supported by a collaboration platform called Trello. Survey was conducted by collecting responses from students in Department of Biology Education who participated in the genetics laboratory work. This study was conducted using survey, which was conducted after students had participated in laboratory work for two weeks online using the virtual collaborative platform. The questionnaires measured students’ perspectives on using Trello for collaborating with their team during laboratory work. The instrument also measured students’ perception on how Trello supported their collaborative learning online including effective group teamwork, discussion activities, and communication within group such as asking questions, answering questions, and making arguments. A number of 127 valid responses were analyzed descriptively. The result showed that students agreed that Trello was easy to use (97%) and supported them to conduct group work easily (98%) and discussion more effectively (87%). Result from this study provides positive evidence on how Trello supports students’ collaboration during laboratory work.

Co-Authors ,, Wirdateti ,, Wirdateti Agung Wijaya Subiantoro Anazifa, Rizqa Devi Anazifa, Rizqa Devi Anggi Tias Pratama Arief Priyadi Asih, Ni Putu Sri Dwi Ayuningtyas Evy Yulianti Fajar Prasetya Ibnu Pratomo isnaeni, bekti Ixora Sartika Mercuriani Kartika Ratna Pertiwi Khan, Farwa Hafeez Lailani, Zhafira Istiqla Lili Sugiyarto Lili Sugiyarto Lili Sugiyarto Lili Sugiyarto Md Hoirul Azri Ponari Mohd Syafik Mohamad Hamdan Mohd Syahmi Salleh Mualimin Mualimin Muhamad Fahmi Yunus Paidi Paidi Pratiwi, Risha Amilia Rizka Anisa Rennytasari Rizka Apriyani Putri Selma Nafilatul Husna Shafila Sahnaz Suratsih Suratsih Tien Aminatun Tutiek Rahayu Wiharyanti, Risma Wiharyanti, Risma Wulandari*, Novia Dian Kartika Wulandari, Sri Wijayanti Wulandari, Sri Wijayanti Zain, Haniza Hanim Mohd

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