Garuda - Garba Rujukan Digital

Biochemical and Fe-resistant characteristics of indigene bacteria from a high iron concentration landfill in Indonesia Saputri, Purwati; Harahap, Diannita; Lubis , Syafrina S; Ilhami, Syarifa
Narra X Vol. 1 No. 3 (2023): December 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v1i3.95

Bacteria isolated from leachate with high heavy metal concentration mostly have potential for bioremediation. The aim of this study was to isolate bacteria from leachate of a landfill containing high concentration of Fe. The leachate sample was collected from Gampong Jawa Landfill, Aceh, Indonesia and used to isolate the bacteria by spread method. The purification of the isolates was carried out through four quadrant plate method. Pure isolates were characterized based on the microscopic or macroscopic appearance and biochemical tests. Fe-resistance was tested by disc diffusion method and constructing the growth curve based on the optical density of broth culture media. A total of 27 pure isolates were obtained and identified, representing eight different genera: Pseudomonas, Bacillus, Neisseria, Micrococcus, Staphylococcus, Proteus, Acinetobacter, and Escherichia. Of eight isolates (each genus was represented by a single isolate), Pseudomonas sp. and Bacillus sp. were considered resistant to Fe exposure (15 ppm) with inhibition zones of 1.00 and 0.81 mm, respectively. At Fe concentration of 20 ppm the inhibition zones exceeded 1 mm, but Bacillus sp. had smaller inhibition zone than that of Pseudomonas sp. (1.15 mm versus 2.86 mm). Growth inhibition of Bacillus sp. was concentration-dependent on Fe exposure but could survive at Fe exposure up to 20 ppm. In conclusion, Bacillus sp. had a resistance against Fe exposure and its bioremediation potential is subjected for future studies.

Antagonistic activity of Trichoderma sp. against pathogens in the leaves of Allium ascalonicum L. Maulana, Indra; Lubis, Syafrina S.; Harahap, Diannita; Arskadius, Nasyaya U.; Concepcion, Ronnie S.
Narra X Vol. 2 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v2i1.125

Pathogenic fungi pose constraints and reduce shallot production. Trichoderma sp. is an antagonistic fungus capable of controlling pathogen growth in shallots. The aim of this study was to determine the characteristics of Trichoderma sp. and pathogens in shallots and to assess the antagonistic ability of Trichoderma sp. against shallot pathogens (Allium ascalonicum L.). Trichoderma sp. and shallot pathogens were isolated using a serial dilution agar plate method using potato dextrose agar medium with 7-day incubation. The antagonistic activity of Trichoderma sp. against shallot pathogens was evaluated based on the dual culture method. In this study, we successfully isolated Trichoderma harzianum from the shallot leaf and its root systems. Moreover, four morphologically distinctive pathogens from shallot roots and leaves were successfully isolated (l Aspergillus sp., Colletotrichum sp., Phytium sp. and Penicillium sp. T. harzianum was found to have the ability to inhibit 23.45% growth of Aspergillus sp, 26.19% growth of Colletotrichum sp., 75.40% growth of Phytium sp., and 40.38% growth of Penicillium sp. In conclusion, the isolated T. harzianum had a strong antagonistic activity against some pathogens in the shallot, but the activity was weak against some others.

IDENTIFIKASI SENYAWA FORMALIN DENGAN ANALISIS KUALITATIF PADA IKAN ASIN DI PASAR TRADISIONAL KABUPATEN ACEH TENGGARA Marwah, Sanasti; Nirmala Sari, Ayu; Harahap, Diannita
AMINA Vol. 6 No. 2 (2024): August 2024
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/amina.v6i2.6360

Kabupaten Aceh Tenggara merupakan salah satu kabupaten yang terletak di Provinsi Aceh, yang memiliki pasar tradisional diantaranya, Pasar Senin, Pasar Simpang, Pasar Lawe Sigala dan Pasar Pajak Pagi. Pasar ini merupakan pasar yang paling banyak dikunjungi masyarakat, karena banyak menjual berbagai macam jenis ikan asin, sehingga banyak diminati masyarakat. Ikan asin memiliki kandungan komposisi asam lemak tidak jenuh, sehingga mengakibatkan terjadinya pembusukan yang mempercepat pertumbuhan jamur dan bakteri. Penggunaan bahan tambahan pangan dalam mengawetkan ikan asin sering sekali disalahgunakan dengan menambahkan bahan kimia seperti formalin. Formalin jika dikonsumsi dapat menganggu kesehatan dengan menimbulkan gejala diare, muntah, sakit kepala, gangguan pernapasan, hipotensi, mutagen dan karsinogen. Tujuan penelitian ini untuk mengetahui kandungan formalin pada ikan asin yang dijual di pasar-pasar tradisional Kabupaten Aceh Tenggara. Pengambilan sampel dilakukan di Pasar Senin, Pasar Simpang, Pasar Lawe Sigala dan Pasar Pajak Pagi, dengan jumlah sampel sebanyak 68. Metode pengujian sampel terdiri dari 4 metode yaitu, asam kromatofat, kalium permanganat, filtrat kulit buah naga dan kit formalin merk ET. Hasil penelitian membuktikan sebanyak 19 sampel positif mengandung formalin, sedangkan 49 sampel terbukti negatif formalin. Jenis-jenis ikan asin yang mengandung formalin yaitu ikan hiu, kepala batu sisik belah, teri, teri medan, peda, perak dan udang rebon.

Biochemical and Fe-resistant characteristics of indigene bacteria from a high iron concentration landfill in Indonesia Saputri, Purwati; Harahap, Diannita; Lubis , Syafrina S; Ilhami, Syarifa
Narra X Vol. 1 No. 3 (2023): December 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v1i3.95

Bacteria isolated from leachate with high heavy metal concentration mostly have potential for bioremediation. The aim of this study was to isolate bacteria from leachate of a landfill containing high concentration of Fe. The leachate sample was collected from Gampong Jawa Landfill, Aceh, Indonesia and used to isolate the bacteria by spread method. The purification of the isolates was carried out through four quadrant plate method. Pure isolates were characterized based on the microscopic or macroscopic appearance and biochemical tests. Fe-resistance was tested by disc diffusion method and constructing the growth curve based on the optical density of broth culture media. A total of 27 pure isolates were obtained and identified, representing eight different genera: Pseudomonas, Bacillus, Neisseria, Micrococcus, Staphylococcus, Proteus, Acinetobacter, and Escherichia. Of eight isolates (each genus was represented by a single isolate), Pseudomonas sp. and Bacillus sp. were considered resistant to Fe exposure (15 ppm) with inhibition zones of 1.00 and 0.81 mm, respectively. At Fe concentration of 20 ppm the inhibition zones exceeded 1 mm, but Bacillus sp. had smaller inhibition zone than that of Pseudomonas sp. (1.15 mm versus 2.86 mm). Growth inhibition of Bacillus sp. was concentration-dependent on Fe exposure but could survive at Fe exposure up to 20 ppm. In conclusion, Bacillus sp. had a resistance against Fe exposure and its bioremediation potential is subjected for future studies.

Antagonistic activity of Trichoderma sp. against pathogens in the leaves of Allium ascalonicum L. Maulana, Indra; Lubis, Syafrina S.; Harahap, Diannita; Arskadius, Nasyaya U.; Concepcion, Ronnie S.
Narra X Vol. 2 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v2i1.125

Pathogenic fungi pose constraints and reduce shallot production. Trichoderma sp. is an antagonistic fungus capable of controlling pathogen growth in shallots. The aim of this study was to determine the characteristics of Trichoderma sp. and pathogens in shallots and to assess the antagonistic ability of Trichoderma sp. against shallot pathogens (Allium ascalonicum L.). Trichoderma sp. and shallot pathogens were isolated using a serial dilution agar plate method using potato dextrose agar medium with 7-day incubation. The antagonistic activity of Trichoderma sp. against shallot pathogens was evaluated based on the dual culture method. In this study, we successfully isolated Trichoderma harzianum from the shallot leaf and its root systems. Moreover, four morphologically distinctive pathogens from shallot roots and leaves were successfully isolated (l Aspergillus sp., Colletotrichum sp., Phytium sp. and Penicillium sp. T. harzianum was found to have the ability to inhibit 23.45% growth of Aspergillus sp, 26.19% growth of Colletotrichum sp., 75.40% growth of Phytium sp., and 40.38% growth of Penicillium sp. In conclusion, the isolated T. harzianum had a strong antagonistic activity against some pathogens in the shallot, but the activity was weak against some others.

Alternative Antibacterial Agents from Endophytic Bacteria of Curry Leaves (Murraya koenigii L. Spreng) for Inhibiting Diarrhea-Causing Bacteria Harahap, Diannita; Firda, Rauzatul
International Journal of Science, Technology and Applications Vol 3 No 1 (2025): Volume 3 Number 1 Tahun 2025
Publisher : Alpatih Harapan Semesta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70115/ijsta.v3i1.269

This study focused on the isolation and characterization of endophytic bacteria from curry leaves (Murraya koenigii L. Spreng), a medicinal plant known for its health-promoting properties. The research aimed to identify bacterial isolates based on their colony morphology, biochemical characteristics, Gram and endospore staining, and to assess their antibacterial activity against Bacillus cereus ATCC 11788 and Shigella sonnei. Leaf segments (1 cm) were surface-sterilized using 2% sodium hypochlorite three times and cultured on Nutrient Agar supplemented with 0.01% nystatin, followed by incubation at 37°C for 24 hours. A total of nine bacterial isolates were obtained, all showing Gram-positive reactions and positive endospore formation, indicating their classification within the Bacillus genus. The antibacterial activity was tested using the Kirby-Bauer disk diffusion method, revealing that the endophytic isolates exhibited weak inhibitory effects against both tested bacterial strains. These findings suggest that while Bacillus endophytes from M. koenigii possess limited antibacterial potential under the tested conditions, they still hold promise for further investigation as potential sources of bioactive compounds with therapeutic applications.

EFFECT EFFECT OF ADDITION OF CHITOSAN AND GLYCEROL ON BIOPLASTICS ORIGINATED FROM MELINJO SEEDS (Gnetum gnemon) Nurraihan , Riska Putri; Kamaliah; Harahap, Diannita; Nisah, Khairun
SciencePlus Vol. 1 No. 1 (2025): SciencePlus
Publisher : Barkah Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2025/sp.v1i1.259

One solution to the plastic waste problem is to produce environmentally friendly plastic made from melinjo starch (Gnetum gnemon). The objective of this research is to determine whether bioplastic made from melinjo seeds (Gnetum gnemon) can be used as a starch-based material for producing eco-friendly plastic and to assess the impact of adding chitosan and glycerol to bioplastic derived from melinjo seeds (Gnetum gnemon). The research method employed is a qualitative and quantitative descriptive analysis of the resulting bioplastic, which consists of seven stages: starch production, bioplastic production, solubility test, swelling test, water resistance test, moisture content test, and biodegradability test. The results show that the composition with 4 grams of melinjo seed starch without the addition of chitosan and glycerol has low solubility (0%) and swelling (2.63%), high water resistance (97.37%), low moisture content (1.97%), and optimal biodegradation rate (97%). The addition of chitosan and glycerol affects the properties of bioplastic derived from melinjo seeds. This addition can increase solubility (2%), reduce the swelling percentage (1.47%), increase moisture content (3.11%), enhance water resistance (98.53%), and reduce the biodegradability percentage of bioplastic (97%).

Title

Found 7 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 7 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 7 Documents
Search