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Ilhami, Syarifa
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Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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Biochemical and Fe-resistant characteristics of indigene bacteria from a high iron concentration landfill in Indonesia Saputri, Purwati; Harahap, Diannita; Lubis , Syafrina S; Ilhami, Syarifa
Narra X Vol. 1 No. 3 (2023): December 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v1i3.95

Abstract

Bacteria isolated from leachate with high heavy metal concentration mostly have potential for bioremediation. The aim of this study was to isolate bacteria from leachate of a landfill containing high concentration of Fe. The leachate sample was collected from Gampong Jawa Landfill, Aceh, Indonesia and used to isolate the bacteria by spread method. The purification of the isolates was carried out through four quadrant plate method. Pure isolates were characterized based on the microscopic or macroscopic appearance and biochemical tests. Fe-resistance was tested by disc diffusion method and constructing the growth curve based on the optical density of broth culture media. A total of 27 pure isolates were obtained and identified, representing eight different genera: Pseudomonas, Bacillus, Neisseria, Micrococcus, Staphylococcus, Proteus, Acinetobacter, and Escherichia. Of eight isolates (each genus was represented by a single isolate), Pseudomonas sp. and Bacillus sp. were considered resistant to Fe exposure (15 ppm) with inhibition zones of 1.00 and 0.81 mm, respectively. At Fe concentration of 20 ppm the inhibition zones exceeded 1 mm, but Bacillus sp. had smaller inhibition zone than that of Pseudomonas sp. (1.15 mm versus 2.86 mm). Growth inhibition of Bacillus sp. was concentration-dependent on Fe exposure but could survive at Fe exposure up to 20 ppm. In conclusion, Bacillus sp. had a resistance against Fe exposure and its bioremediation potential is subjected for future studies.
Fabrication and characterization of SiO2-embedded castor oil-based membrane (Ricinus communis L) for aqueous Fe adsorption Nisah, Khairun; Khairi, Miratul; Sukandar , Redha; Nuzlia, Cut; Nasution, Reni S.; Ilhami, Syarifa; Chiari, Williams
Narra X Vol. 2 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v2i2.158

Abstract

Castor seed oil (Ricinus communis L) can be utilized for the manufacturing of membranes reacted with toluene diisocyanate (TDI). The aim of this study was to examine the effect and membrane characteristics of castor seed oil with a combination of silica used as an adsorbent, with a combination of silica (SiO2) 0.5 mg, 1 mg, 1.5 mg, and 2 mg. The initial analysis of the membrane was carried out with a swelling test of 1.5 mg of silica variation to obtain the most ideal result of 200%. The best chemical resistance characterization occurred at a variation of 1.5 mg of silica. Further characterization, particularly the FTIR test, thermal test, mechanical test, and SEM test, showed that there were Si-O groups and amine groups (NH2), and on the membrane there were groups OH, C=O, and NH. The thermal characterization of the silica membrane (1.5 mg) gave the greatest residue concentration, measuring 3.2%. In mechanical terms, the silica membrane has a higher elongation value than the membrane. In SEM characterization, the silica membrane has holes and is solid. This study indicated that the highest drop in Fe metal occurred at an immersion period of 6 hours with a combination of silica with a flux value of 9.25 L/m2 hour and a rejection value of 0.098%.
Biochemical and Fe-resistant characteristics of indigene bacteria from a high iron concentration landfill in Indonesia Saputri, Purwati; Harahap, Diannita; Lubis , Syafrina S; Ilhami, Syarifa
Narra X Vol. 1 No. 3 (2023): December 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v1i3.95

Abstract

Bacteria isolated from leachate with high heavy metal concentration mostly have potential for bioremediation. The aim of this study was to isolate bacteria from leachate of a landfill containing high concentration of Fe. The leachate sample was collected from Gampong Jawa Landfill, Aceh, Indonesia and used to isolate the bacteria by spread method. The purification of the isolates was carried out through four quadrant plate method. Pure isolates were characterized based on the microscopic or macroscopic appearance and biochemical tests. Fe-resistance was tested by disc diffusion method and constructing the growth curve based on the optical density of broth culture media. A total of 27 pure isolates were obtained and identified, representing eight different genera: Pseudomonas, Bacillus, Neisseria, Micrococcus, Staphylococcus, Proteus, Acinetobacter, and Escherichia. Of eight isolates (each genus was represented by a single isolate), Pseudomonas sp. and Bacillus sp. were considered resistant to Fe exposure (15 ppm) with inhibition zones of 1.00 and 0.81 mm, respectively. At Fe concentration of 20 ppm the inhibition zones exceeded 1 mm, but Bacillus sp. had smaller inhibition zone than that of Pseudomonas sp. (1.15 mm versus 2.86 mm). Growth inhibition of Bacillus sp. was concentration-dependent on Fe exposure but could survive at Fe exposure up to 20 ppm. In conclusion, Bacillus sp. had a resistance against Fe exposure and its bioremediation potential is subjected for future studies.
Fabrication and characterization of SiO2-embedded castor oil-based membrane (Ricinus communis L) for aqueous Fe adsorption Nisah, Khairun; Khairi, Miratul; Sukandar , Redha; Nuzlia, Cut; Nasution, Reni S.; Ilhami, Syarifa; Chiari, Williams
Narra X Vol. 2 No. 2 (2024): August 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narrax.v2i2.158

Abstract

Castor seed oil (Ricinus communis L) can be utilized for the manufacturing of membranes reacted with toluene diisocyanate (TDI). The aim of this study was to examine the effect and membrane characteristics of castor seed oil with a combination of silica used as an adsorbent, with a combination of silica (SiO2) 0.5 mg, 1 mg, 1.5 mg, and 2 mg. The initial analysis of the membrane was carried out with a swelling test of 1.5 mg of silica variation to obtain the most ideal result of 200%. The best chemical resistance characterization occurred at a variation of 1.5 mg of silica. Further characterization, particularly the FTIR test, thermal test, mechanical test, and SEM test, showed that there were Si-O groups and amine groups (NH2), and on the membrane there were groups OH, C=O, and NH. The thermal characterization of the silica membrane (1.5 mg) gave the greatest residue concentration, measuring 3.2%. In mechanical terms, the silica membrane has a higher elongation value than the membrane. In SEM characterization, the silica membrane has holes and is solid. This study indicated that the highest drop in Fe metal occurred at an immersion period of 6 hours with a combination of silica with a flux value of 9.25 L/m2 hour and a rejection value of 0.098%.
Co-Authors Chiari, Williams Harahap, Diannita Khairi, Miratul Khairun Nisah Lubis , Syafrina S Nasution, Reni S. Nuzlia, Cut Saputri, Purwati Sukandar , Redha