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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Promkes: The Indonesian Journal of Health Promotion and Health Education Jurnal Surya Medika Global Health Management Journal Journal Midwifery Jurusan Kebidanan Politeknik Kesehatan Gorontalo EJOIN : Jurnal Pengabdian Masyarakat
Natalina, Riny
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences

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The Effect of Human Pellucide Zone 3 Monoclonal Antibody on Expression of Bcl-2 and Bax in Follicle Granulosa Cells of Mice Ovary Natalina, Riny; Nurseta, Tatit; Winarsih, Sri
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.255 KB) | DOI: 10.11594/jtls.08.02.09

Abstract

Pellucide zone 3 (ZP3) involves in fertilization mechanism. Moreover, antibody of ZP3 can develop to inhibit egg and sperm interaction. This study aims to determine the effect of hZP3 (mab-hZP3) monoclonal antibody on the expression of Bcl-2 and Bax in follicle granulosa cells of the mice ovary. Female mice BALB/c were divided into 12 groups which consisted of control and experimental treatment group. Each group was added with 30% of total mice as error sample (1 mice). Each groups were treated differently: 50 µl adjuvant Al(OH)3 in 50 µl Tris HCl, 20 µg Mab-hZP3, 40 µg Mab- hZP3, and 60 µg Mab-hZP3. Each group was dissected at day 10, 15 and 20. Measurement of Bcl-2 and Bax was performed with immunohistochemistry. Data was analyzed by Two-Way ANOVA. There was no significant effect of Mab-hZP3 administration in various doses on Bcl-2 (p=0.0825), and Bax (p=0.836). There was no significant effect of administration of Mab-hZP3 in time (p=0.807), neither on Bcl-2 expression (p=0.088) and Bax (p=0.227). The lowest Bcl-2 level was found in dose of 60 µg in day 15. There was no significant effect of Mab-hZP3 in various doses and time (p=0.691), neither to Bcl-2 and Bax. Such results obtained due to the specificity of a monoclonal antibody that recognizes specific antigen. Mab-hZP3 is proposed as immunocontraception for women causing no disturbance of folliculogenesis.
Co-Authors A.A. Ketut Agung Cahyawan W Damiti, Sukmawaty A Fastika, Cindy Hatini, Erina Eka Legawati Legawati Legawati, Legawati Muhamad Ratodi Oktaviani , Oktaviani Setyaningsih, Eni Tatit Nurseta Wahyuni, Sery