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Pengaruh air kelapa sebagai sitokinin organik dan sukrosa terhadap pertumbuhan protocorm anggrek (Phalaenopsis hybrid MP 253 x F1 3363 (M)) in vitro Muhammad Isyraq; Lia Amalia; Iis Aisyah
Kultivasi Vol 20, No 1 (2021): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v20i1.31941

Abstract

Abstrak. Sitokinin dan sukrosa dibutuhkan untuk meregenerasi protocorm anggrek. Air kelapa diketahui memiliki kandungan sitokinin sehingga berpotensi dijadikan sebagai sitokinin organik. Percobaan ini bertujuan untuk mempelajari pengaruh konsentrasi air kelapa sebagai sitokinin organik dan sukrosa terhadap pertumbuhan protocorm anggrek. Percobaan ini dilaksanakan di Laboratorium Bioteknologi Tanaman, Fakultas Pertanian Universitas Winaya Mukti, Kecamatan Tanjungsari Kabupaten Sumedang. Percobaan dimulai pada bulan Mei sampai dengan bulan Agustus 2019. Rancangan percobaan yang digunakan adalah rancangan acak kelompok dengan dua faktor dan diulang dua kali. Faktor pertama adalah konsentrasi air kelapa, terdiri dari lima taraf yaitu 0 mL L-1, 50 mL L-1, 100 mL L-1, air kelapa 150 mL L-1, dan air kelapa 200 mL L-. Faktor kedua adalah konsentrasi sukrosa dengan lima taraf, yaitu 0 g L-1, 10 g L-1, 20 g L-1, 30 g L-1 dan 40 g L-1. Hasil percobaan menunjukkan terjadi pengaruh interaksi pada pengamatan jumlah daun umur 12 MST dan bobot segar protocorm. Perlakuan air kelapa 50 mL L-1 dengan tanpa sukrosa menunjukkan jumlah daun yang paling baik dari perlakuan lainnya. Bobot segar protocorm menunjukkan hasil yang terbaik pada umur 12 MST pada konsentrasi air kelapa 100 mL L-1 dengan tanpa sukrosa.Kata kunci: Air Kelapa, In vitro, Protocorm anggrek, Sukrosa. Abstract. Cytokinins and sucrose are needed to regenerate orchid protocorms. Coconut water contains cytokinin so that it has the potential to be used as organic cytokinin. This experiment aims to study the effect of coconut water and sucrose concentrations on the growth of orchid protocorms. This experiment was carried out at the Plant Biotechnology Laboratory, Faculty of Agriculture, University Winaya Mukti Tanjungsari, Sumedang. The experiment was conducted from May to August 2019. The experimental design used a randomized block design with two factors and was repeated twice. The first factor was coconut water concentration, consisted by five levels: 0 mL L-1, 50 mL L-1, 100 mL L-1, 150 mL L-1, and 200 mL L-1. The second factor was sucrose concentration, consisted by five levels: 0 mL L-1, 10 g L-1, 20 g L-1, 30 g L-1 and 40 g L-1. The results of the experiment showed that there were the interaction effect of coconut water and sucrose concentrations on the number of leaves and fresh weight of protocorms. Treatment of 50 mL L-1 coconut water treatment with no sucrose showed the best number of leaves, compared to other treatments. The best fresh weight of protocorm was given by treatment of coconut water 100 mL L-1 without sucrose.Keywords: Coconut water, In vitro, Orchid protocorm, Sucrose.
Pengaruh posisi bukaan plastik baglog dan konsentrasi pupuk fosfor terhadap pertumbuhan dan hasil jamur tiram putih (Pleurotus ostreatus) Lia Amalia; R Budiasih; Asep Samsul
Kultivasi Vol 17, No 1 (2018)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (771.133 KB) | DOI: 10.24198/kultivasi.v17i1.16075

Abstract

Abstract. The decrease in mushroom produc-tivity due to openings at the top of the baglog needs to be balanced with phosphor to increase the mushroom harvest. The research study the interaction effect of opening position plastic baglogs and concentration of phosphor fertilizers to growth and yield of oyster mushroom. The experiment has been done in Mushrooms House, Faculty of Agriculture, Winaya Mukti University, Sumedang with altitude of 850 m above sea level. It was carried out  from  September until December 2015. The experimental design used in this experiment was completely randomized design and treatment design was factorial. The treatment consisted of two factors: the opening position of plastic baglogs and fertilizer concentrations of phosphor fertilizer. Opening position of plastic baglogs consists of a 6 levels that b1 = vertically baglog, the top of baglog opened entirely, b2 = vertically baglog, left side baglog opened at areas 3cm x 1cm, b3 = vertically baglog, left and right side baglog opened at areas 3cm x 1cm, b4 = horizontally baglog, left and right side baglog opened entirely, b5 =  the left side baglog  opened at areas 3cm x 1cm, and b6 = left and right side baglog opened at areas 3cm x 1cm. The concentration of phosphor fertilizer consisted of 3 levels that p0 = 0 g L-1 solution, p1 = 0.25 g L-1 solution, and p2 = 0.50 g L-1 solution. All treatment was replicated 2 times. The results showed that there was interaction between  opening position of plastic baglog and a concentration of phosphor fertilizer to height of mushroom fruiting bodies.Keywords: Baglog, Oyster Mushrooms, Phosphor Sari. Penurunan produktivitas jamur akibat bukaan plastik pada bagian atas baglog perlu diimbangi dengan pemberian fosfor untuk meningkatkan hasil panen jamur tiram. Penelitian bertujuan untuk mempelajari pengaruh interaksi  posisi bukaan plastik baglog dan konsentrasi pupuk fosfor terhadap pertumbuhan dan hasil jamur tiram putih. Percobaan telah dilakukan di Kumbung Jamur Fakultas Pertanian Universitas Winaya Mukti (UNWIM), Kabupaten Sumedang dengan ketinggian tempat 850 m di atas permukaan laut dan dilaksanakan mulai dari bulan  September  sampai bulan Desember 2015. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan rancangan perlakuan adalah faktorial. Perlakuan terdiri dari dua faktor yaitu posisi bukaan plastik  baglog dan konsentrasi pupuk fosfor.  Posisi bukaan plastik baglog  terdiri dari 6 taraf faktor yaitu b1 = baglog diberdirikan, bagian atas baglog dibuka  seluruhnya, b2  = baglog diberdirikan, samping kiri baglog bagian tengahnya dibuka seluas 3cm x 1cm, b3  = baglog diberdirikan, samping kiri dan kanan baglog bagian tengahnya dibuka seluas 3cm x 1cm,  b4  = baglog ditidurkan, samping kiri dan kanan baglog dibuka seluruhnya, b5 = baglog ditidurkan, samping kiri baglog bagian tengahnya dibuka seluas 3cm x 1 cm, dan b6 = baglog ditidurkan, samping kiri dan kanan baglog bagian tengahnya dibuka seluas 3cm x 1 cm  . Konsentrasi pupuk fosfor terdiri dari 3 taraf faktor yaitu p0 = 0 g L-1 Larutan, p1  = 0,25 g L-1 Larutan , dan p2 = 0,50 g L-1 Larutan, sehingga terdiri dari 18 kombinasi perlakuan yang diulang sebanyak 2 kali. Hasil percobaan menunjukkan terjadi interaksi antara posisi bukaan plastik baglog dengan konsentrasi pupuk fosfor terhadap tinggi tubuh buah jamur.Kata kunci : Baglog, Fosfor, Jamur Tiram
The Effect of Bamboo’s Root PGPR Concentration and Row Spacing on Growth and Yield of Kidney Bean (Phaseolus vulgaris) R. Budiasih; Lia Amalia; Yuli Nurfatwa
Jurnal Sains Dasar Vol 7, No 2 (2018): October 2018
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jsd.v7i2.38550

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The experiment aims to know and learn an interaction of bamboo’s root concentration and row spacing on growth and yield of kidney bean (Phaseolus vulgaris). This experiment was carried out at Gunungmanik Village, in the sub district Tanjungsari Sumedang from May until July 2016. The altitude is about 800 meters above sea level, soil order andisol with pH 6,41 and types of rainfall is C (dampy). Design was used as Randomized Block Design (RBD) composed of two factor treatments with two replications. First factor was concentration of PGPR (Plant Growth Promoting Rhizobacteria) bamboo’s root with four level of treatment, namely is  p0 = 0 ml L-1 solution, p1 = 5 ml L-1 solution, p2 = 10 ml L-1 solution, and p3 = 15 ml L-1 solution and row spacing with four level of treatments, namely is : j1 = 20 cm x 20 cm, j2 = 30 cm x 20 cm, j3 = 40 cm x 20 cm, dan j4 = 50 cm x 20 cm. The result of the experiment showed there are interaction between concentration of PGPR bamboo’s root and row spacing to plant height age 35 DAP. The result observation of growth shows the highest of plant height on giving concentration of PGPR bamboo’s root 15 ml L-1 solution accompanied row spacing 40 cm x 20 cm (p3j3) that is 74,1 cm, while on observation result shows wet weight of the seeds on giving concentration PGPR bamboo’s root 5 ml L-1 solution that is 3259,27 g and the use of  row spacing 20 cm x 20 cm that is 4222,69 g. The effects bamboo’s root PGPR concentration  and row spacing to plant height, number of leaves, numbers of pod per plot, numbers of seed per plot, wet weight of the seeds (pods), dry weight of the seeds (pods), wet weight of the seeds (without pods), dry weight of the seeds (without pods), and weight of 100 grains.
Efek Konsentrasi Pupuk Organik Cair dan Jarak Tanam Terhadap Karakter Agronomi dan Serapan N Pada Tanaman Selada Hijau (Lactuca sativa L.) Sri Endang Agustine; Lia Amalia; Tien Turmuktini
COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat Vol. 1 No. 11 (2022): COMSERVA : Jurnal Penelitian dan Pengabdian Masyarakat
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (966.73 KB) | DOI: 10.59141/comserva.v1i11.174

Abstract

This study intends to study the interaction of Liquid Organic Fertilizer concentration and plant spacing on agronomic characters and N uptake in lettuce. It was carried out in the experimental area of ??Chemical Agro Lembang, West Bandung Regency and carried out from September 2021 to October 2021 with an altitude of 1250 m above sea level. The experimental method used was a factorial randomized block design (RAK). The first factor was the POC concentration consisting of 3 levels, namely 0, 2 and 4 ml L-1 and the second factor was the spacing between 3 levels, namely 30 cm x 25 cm, 25 cm x 25 cm and 20 cm x 25 cm. The results of the study showed that there was no interaction between POC concentration and plant spacing in observations. Giving a concentration of 4 ml L-1 POC and plant spacing with treatment of 30 cm x 25 cm gave the highest yield on agronomic characters and N uptake in lettuce. The optimum POC concentration for each plant spacing in the treatment (P1) which was 0.774 tons h-1 was able to increase the maximum yield of fresh plant weight by 95.93 g per plant, the concentration (P2) which was 2.145 tons h-1 was able to increase the maximum yield of plant weight. fresh weight per plant as much as 162.18 g per plant and the optimum concentration for (P3) which is 2.48 tons ha-1 can increase the maximum yield of fresh weight per plant as much as 131.30 g per plant.
PENGUJIAN EFEKTIVITAS WAKTU PEMANGKASAN BUNGA JANTAN TERHADAP PENINGKATAN KOMPONEN HASIL DAN HASIL JAGUNG MANIS (Zea may L) Lia Amalia
Paspalum: Jurnal Ilmiah Pertanian Vol 5, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.777 KB) | DOI: 10.35138/paspalum.v5i1.34

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The research was conducted in experimental garden of Faculty of Agriculture Unwim which has a place to stay 850 mdpl. This study aims to determine the effective timing of male pruning to increase yield components and sweet corn yield. The environmental plan used is a collection of groups consisting of: A = Without pruning B = Pruning 7 days after pollination C = Pruning 14 days after pollinationD = Pruning 21 days after pollination. The result of the research showed that the male flower pruning after pollination of the effect on corn yield and yield components such as Weight, Length and Diameter of Tonggol both without and with kelobot. Sweet corn plants that are not exposed to male flower pruning have the same result and yield components as the plants that are trimmed by their male flowers
Korelasi Beberapa Karakter Biokimia dengan Ketahanan Tanaman cabai terhadap Penyakit Antraknos yang Disebabkan Colletotruchun gloeosporioides Lia Amalia
Paspalum: Jurnal Ilmiah Pertanian Vol 1, No 1 (2011)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3111.36 KB) | DOI: 10.35138/paspalum.v1i1.39

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Penelitian untuk mengetahui korelasi antar karakter  biokimia dengan katahanan cabai terhadap penyakit Antraknos. Penelitian dilakukan di Kebun Percobaan dan Laboratorium Hama dan Penyakit Fakultas Pertanian UNWIM, Tanjungsari Sumedang dari bulan Oktober 2003 - Februari 2004. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan jumlah ulangan tidak sama, dengan asumsi model acak. Hasil penelitian menunjukkan bahwa baik dilapangan maupun di laboratorium, karakter kadar air buah mempunyai nilai korelasi fenotip dan genetik yang tidak nyata dengan intensitas penyakit Antraknos. Karakter kadar antosianin dan kadar lignin kulit buah berkorelasi fenotip negatif nyata terhadap intensitas penyakit antraknos, tetapi berkorelasi genetik negatif tidak nyata terhadap intensitas penyakit Antraknos. Hal ini menunjukkan bahwa penerapan seleksi tidak langsung secara genetik untuk karakter ketahanan tanaman cabai merah melalui karakter kadar antosianin, kadar air dan kadar lignin kulit buah tidak akan efektif.
Respon Pertumbuhan dan Hasil Tanaman Kacang Tanah (Arachis hypogaea L.) akibat Pemberian Pupuk Organik Cair dan Jarak Tanam Lia Amalia; Nunung Sondari; Nana Supriatna; Reni Nurhayatini; Agus Surya Mulya; Nendah Siti Permana
Paspalum: Jurnal Ilmiah Pertanian Vol 9, No 2 (2021)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35138/paspalum.v9i2.284

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Peanut production has decreased, among others, due to cultivation techniques, varieties, diseases and increasingly narrow planting areas. One of the cultivation techniques to increase crop yields is the use of liquid organic fertilizer (POC) and spacing. The research method used was Randomized Block Design, factorial pattern. Consisting of two treatment factors, namely POC concentration (0, 5, 10, and 15 ml L-1 water) and spacing (30 cm x 10 cm, 30 cm x 15 cm and 30 cm x 20 cm), repeated three times. .. The results showed that: (1) there was an interaction between POC treatment and plant spacing on the number of pithy seeds and dry seed weight per plot. Independently, liquid organic fertilizer and plant spacing had an effect on plant height, number of leaves, dry weight of stover, number of filled pods, number of empty pods, percentage of filled pods and weight of 100 seeds, and (2) At a spacing of 30 cm x 15 cm, The optimum POC concentration was 13.24 ml L-1 of water, with a maximum dry seed weight of 1.436 kg plot-1 or equivalent to 2.66 tons ha-1. 
Komposisi Amelioran Terhadap Pertumbuhan Dan Hasil Tanaman Kentang G1 (Solanum Tuberosum L.) Varietas Medians Linlin Parlinah; Hudaya Mulyana; Lia Amalia; Nunung Sondari
Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech) Vol 5 No 1 (2020): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v5i1.3546

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This study aims to study the influence of the use of doses and composition of amelioran on the growth and yield of the potato varieties medians (Solanum tuberusum L.). The experiment was carried out in the Greenhouse Faculty of Agriculture University Winaya Mukti Tanjungsari Sumedang with a height of 850 m above sea level and the land order Andisols. The study was conducted in August to October 2017. The draft used is the group random draft, consisting of nine treatments and each repeated three times. Treatment of composition of Amelioran test is: A: control; B: Cow manure 10 ton ha-1; C: Cow manure 20 ton ha-1; D: Cow Manure 8 tons ha-1 + dolomite 1 ton ha-1 + biochar 1 ton ha-1; E: Cow manure 16 ton ha-1 + dolomite 2 ton ha-1 + biochar 2 ton ha-1; F: Cow Manure 8 tons ha-1 + dolomite 2 ton ha-1; G: Cow Manure 16 tons ha+ + dolomite 4 ton ha-1; H: Cow Manure 8 tons ha+ + biochar 2 ton ha-1; I: Cow manure 16 ton ha-1 + biochar 4 ton ha-1. The results of the experiment showed that the combined dose and composition of amelioran were not real to the growth, the results of potato plants except on tuber diameter. Administration of Ameliorant with a composition of cow manure 16 tons Ha-1 + dolomite 4 ton Ha-1 able to increase the diameter of potato tuber of 58% compared with control treatment.
Keberhasilan Pertumbuhan Stek dan Hasil Bunga Krisan Varietas Puspita Nusantara Akibat Pemberian Dosis Pupuk Kandang Sapi dan Konsentrasi Auksin Lia Amalia; Wahyono Widodo; Anne Berliana; Iis Aisyah; Ai Komariah; Odang Hidayat; Nunung Sondari
Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech) Vol 5 No 2 (2020): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v5i2.4342

Abstract

The availability of nutrients and growth hormones will affect plant growth and yield. Experiments to study the effect of the interaction between cow manure dose and auxin concentration on the success of cuttings growth, growth and yield of Puspita Nusantara chrysanthemum flowers were carried out. The experimental design used was a factorial randomized block design. There are two treatment factors that were repeated 2 times. The first factor is the dose of manure consisting of four levels of 0 t ha-1, 10 t ha-1, 20 t ha-1, and 30 t ha-1. The second factor is the auxin concentration consisting of four levels, namely 0 ml L-1 solution, 1.3 ml L-1 solution, 2.6 ml L-1 solution and 3.9 ml L-1 solution. The results showed that there was an interaction between cow manure dose and auxin concentration on the number of leaves, stem diameter, number of flower buds, flower diameter, flower stalk length, and vaselife. Independently, the dose of cow manure 20 t ha—1, 30 t ha-1 and auxin concentration of 2.6 ml L-1 , 3.9 ml L-1 solution gave higher plant height than the control.
Penggunaan Konsentrasi Ab Mix Dan Vitamin B1 Terhadap Perbanyakan Planlet Kentang (Solanum Tuberosum L.) Varietas Granola Secara In Vitro Lia Amalia; Rahma Winahyu Adi; Kovertina Rakhmi Indriana
Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech) Vol 6 No 2 (2021): Jurnal Agrotek Indonesia (Indonesian Journal of Agrotech)
Publisher : Faculty of Agriculture University of Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33661/jai.v6i2.5434

Abstract

Percobaan ini bertujuan untuk memperlajari penggunaan konsentrasi AB Mix dan vitamin B1 terhadap perbanyakan planlet kentang varietas Granola secara in vitro dan mengetahui konsentrasi AB Mix dan vitamin B1 yang paling efektif untuk digunakan sebagai media tanam alternatif dalam perbanyakan planlet kentang varietas Granola secara in vitro. Percobaan ini dilaksanakan di laboratorium kultur jaringan, Fakultas Pertanian, Universitas Winaya Mukti yang dimulai pada bulan Mei sampai Agustus 2019. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap dengan dua faktor dan diulang dua kali. Faktor pertama konsentrasi AB Mix (A) dengan 5 taraf perlakuan yaitu, a1=600 mg L-1 larutan, a2=1200 mg L-1 larutan, a3=1800 mg L-1 larutan, a4=2400 mg L-1 larutan, a5=3000 mg L-1 larutan, dan faktor kedua konsentrasi vitamin B1 (B) dengan 5 taraf perlakuan yaitu, b0=0 mg L-1 larutan, b1=0,5 mg L-1 larutan, b2=1 mg L-1 larutan, b3=1,5 mg L-1 larutan, dan b4=2,0 mg L-1 larutan. Hasil percobaan ini menunjukkan bahwa tidak terjadi interaksi antara konsentrasi AB Mix dan vitamin B1 terhadap perbanyakan planlet kentang varietas granola secara in vitro. Konsentrasi AB Mix maupun kosentrasi vitamin B1 menunjukkan hasil yang berbeda tidak nyata pada seluruh perlakuan terhadap setiap parameter pengamatan.