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All Journal Specta Journal of Technology Makara Journal of Science
Sholikah, Umi
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Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Electrical & Electronics Engineering Environmental Science

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Efek Kadmium terhadap profil DNA Bacillus cereus ATCC 9632 dan Pseudomonas aeruginosa WT pada Limbah Sintetik Elektroplating Sholikah, Umi; Trihadiningrum, Yulinah
SPECTA Journal of Technology Vol 2 No 2 (2018): SPECTA Journal of Technology
Publisher : LPPM ITK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35718/specta.v2i2.100

Abstract

The electroplating industry produces wastewater containing heavy metals. The high surface area of bacteria has the potency to reduce heavy metals content in wastewater since it has ability to absorb the metals. This study aims to find out the bacterial DNA profile after Cd bioremoval process using Bacillus cereus 9632 and Pseudomonas aeruginosa WT. Bacterial culture is applied in synthetic electroplating wastewater. Some Cd concentrations are used to perform Range Finding Test by bacterial culture. The study was conducted in a batch system for three days. The viability test was done by measuring the value of Optical Density and Total Plate Count, while bacterial DNA profile was analyzed by using electrophoresis. The cell viability of B.cereus ATCC 9632 and P.aeruginosa WT had the same pattern which is the higher concentration of Cd metals on cell bacteria surface, the lower cell bacteria viability. The DNA of the control bacteria has the difference between the size and number of bands.
Application of Microbial Fuel Cells for Leachate Bioremediation: The Effects of Nutrient Nourishment Sholikah, Umi; Chosta, Fidela; Fiqar, Tegar Palyus; Anifah, Eka Masrifatus; Hudayarizka, Riza; Hibino, Tadashi
Makara Journal of Science Vol. 30, No. 1
Publisher : UI Scholars Hub

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Abstract

Leachate contains a high organic content, which can cause environmental pollution. The organic content in leachate can be removed through bioremediation using microbial fuel cell (MFC) systems to remediate pollutants and generate bioelectricity. Organic matter is important to the MFC process as both a substrate and a nutrient source. This study investigated the effects of glucose and sodium phosphate buffer nourishment on BOD, COD, and TSS removal and bioelectricity generation. This study used leachate as a substrate and a graphite rod with a surface area of about 32.98 cm2 as an electrode. The reactor uses plastic blocks with a 3L capacity. Bioremediation using MFC was conducted in batch experiments under aerobic conditions for 20 days at various glucose dosages. All monitoring processes were conducted in real time using the internet of things (IoT). The glucose variations used were 10% (b/v) and 20% (b/v) with the addition of a sodium phosphate buffer. The removal efficiencies of BOD, COD, and TSS with 10% glucose and sodium phosphate buffer addition were 86.05%, 81.84%, and 75.50%, respectively. The highest electrical voltage was observed upon the addition of 20% glucose and sodium phosphate buffer at about 900 mV.
Co-Authors Anifah, Eka Masrifatus Assidiq, Prayudi Brillian Chosta, Fidela Hibino, Tadashi Nurrahman, Farid Riza Hudayarizka Tegar Palyus Fiqar Yulinah Trihadiningrum