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All Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Sinergitas PkM & CSR Jurnal Kridatama Sains dan Teknologi J-HESTECH (Journal Of Health Educational Science And Technology) Jurnal Insan Farmasi Indonesia
Andareas, Pangeran
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health Social Sciences

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Deteksi Genetik Sars-Cov-2 di Swab Lingkungan Asrama Mahasiswa Fakultas Keperawatan Aruan, Maroloan; Andareas, Pangeran; Jo, Juandy
Journal of Health Educational Science And Technology Vol. 6 No. 2 (2023): J-HESTECH (Journal Of Health Educational Science And Technology)
Publisher : Faculty of Health Sciences , Dr. Soetomo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25139/htc.v6i2.6682

Abstract

Pemerintah telah menerapkan serangkaian langkah khusus untuk mengendalikan penyebaran virus SARS-CoV-2 di tempat kerja dan lingkungan sosial. Proses pengendalian ini sangat penting, karena ketidakmampuan untuk menemukan carriers/ pembawa sebelum menunjukkan dan tanpa gejala, dapat mengganggu efektivitas proses pengendalian. Penularan SARS-CoV-2 dari pasien bergejala dan tanpa gejala terjadi melalui droplet yang dikeluarkan saat batuk atau bersin tanpa menutup mulut atau hidung. Namun, hal ini tentu tidak menutup kemungkinan terjadinya infeksi melalui kontak sentuhan pada permukaan benda dan lingkungan. Hipotesis fomite ini dianalisis dengan menguji sampel swab lingkungan menggunakan metode PCR kuantitatif Reverse Transcription (RT-qPCR). Dalam penelitian ini, deteksi genetik yang dilakukan di 13 titik di asrama mahasiswa Universitas Pelita Harapan selama bulan November 2021, enam sampel dinyatakan positif, ditunjukkan dengan Cycle threshold (Ct) RT-qPCR yaitu di lantai 15: lift luar kancing (Ct= 36,69), kancing mesin cuci (Ct=37,24), gagang pintu kamar (Ct=36,79), gagang lemari es (Ct=36,6); di lantai 16: tombol lift luar (Ct=37,47) dan pegangan kursi tunggu lift (Ct=37,84).
POTENTIAL OF CITRUS LEAF EXTRACT (Cymbopogon citratus (DC.) Stapf) AS AN ANTIBACTERIAL INHIBITORY of Staphylococcus epidermidis Karnelasatri, Karnelasatri; Cenwira, Wishia; Andareas, Pangeran; Santoso, Feronia Reni Cyrena
Jurnal Insan Farmasi Indonesia Vol 6 No 2 (2023): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v6i2.1601

Abstract

The concerns of antibiotic resistance have increased in recently years, this issue caused by long-term repeated use of antibiotics to treat bacterial illnesses. Lemongrass leaf (Cymbopogon citratus (DC.) Stapf) is one of the natural ingredients that can be used as an alternative antibacterial. The study objective is to determine the antibacterial activity of the ethanol extract of 70% cooking leaves against Staphylococcus epidermidis using diffusion methods. In addition, the Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of extracts were determined using liquid dilution methods. The results of this study show that extract concentrations of 1,000,000 ppm have a barrier zone in the intermediate category when compared with the positive control, that is, clindamycin. Meanwhile, the MIC value obtained is 1250 ppm, while the MBC value is 5000 ppm. The results of statistical analysis showed that there were no significant differences in the inhibition zone diameter formed between the test concentrations.
Antibacterial Activity Assay on Escherichia coli of The Active Fraction Matoa Seeds (Pometia Pinnata J.R Forst & G. Forst.) Karnelasatri, Karnelasatri; Yacob, Iren Anggreni; Andareas, Pangeran; Santoso, Feronia Reni Cyrena; Novia, Jessica
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 10 No. 2 (2024): (October 2024)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2024.v10.i2.16283

Abstract

One of the plants had been used as a medicinal plant in Papua is Pometia Pinnata J.R Forst & G. Forst or the Matoa plant. The aim of this study is to determine the antibacterial activity of the active fraction of Matoa seeds against Escherichia coli bacteria. The positive control and negative control used were ciprofloxacin and dimethyl sulfoxide, respectively. 96% ethanol solvent was used to extract the simplicia of Matoa seeds. Fractionation was carried out using n-hexane, ethyl acetate and water as solvents. The Kirby Bauer diffusion method was used in the antibacterial activity assay at concentrations of 100 mg/mL, 200 mg/mL, 300 mg/mL, 400 mg/mL, and 500 mg/mL. Overall, the inhibition zones obtained from the sequential concentrations above were 8.89 mm, 10.18 mm, 10.34 mm, 10.84 mm, and 11.76 mm respectively. Further, data analysis was performed with One Way ANOVA and the results showed significant differences between concentrations. Data analysis was then continued with the post hoc Tukey HSD test and a concentration of 500 mg/mL was concluded as the optimum concentration.
TRAINING ON BIOCHEMICHAL IDENTIFICATION OF ENTEROBACTERIACEAE USING THE ENTEROSYSTEM 24R KIT TO STUDENTS OF MEDICAL LABORATORY TECHNOLOGY AT YARSI MEDIKA VOCATIONAL HIGH SCHOOL, TANGERANG Andareas, Pangeran; Aruan, Maroloan; Lusiana, Diana Intan Gabriella; Mardiyono, Maleakhi Samuel; Situmorang, Dolito; Purba, Naomi Damayanti; Tahalele, Nava Anabel
Jurnal Sinergitas PKM & CSR Vol. 8 No. 3 (2024): DECEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tri Dharma Perguruan Tinggi is the main foundation for universities in Indonesia in carrying out their functions and responsibilities. One of the activities that is part of the Tri Dharma of Higher Education is Community Service (CS). PkM activities have the aim of improving the welfare of the community by providing solutions to various problems that exist in the community. One solution that can be provided is to provide training to the community with the aim of increasing knowledge, skills or competencies of human resources (HR).  One of the important communities to improve competence is school students, especially vocational high schools (VHS) majoring in medical laboratory technology (MLT). This activity is important so that the graduates produced are in accordance with the needs of the world of work such as hospitals, clinics and research institutions. This activity was given to students majoring in medical laboratory technology, VHS Yarsi Medika Tangerang. The topic raised in this activity is training in the identification of enterobacteriaceae using the Enterosystem 24R kit. The data in this activity was obtained with a Quiz instrument (Pre-Test and Test) using Google Form.  The results obtained from PkM activities show that there is an increase in student knowledge related to PkM material by 35%.
Keanekaragaman Genetik Kerang Totok (Polymesoda Erosa) Di Sungai Donan Cilacap Berdasarkan Penanda RAPD Andareas, Pangeran
Jurnal Kridatama Sains dan Teknologi Vol 2 No 01 (2020): JURNAL KRIDATAMA SAINS DAN TEKNOLOGI
Publisher : Universitas Ma'arif Nahdlatul Ulama Kebumen

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (605.395 KB) | DOI: 10.53863/kst.v2i01.22

Abstract

Abstrak Kerang totok (Polymesoda erosa) merupakan salah satu jenis kerang yang dimanfaatkan sebagai sumber makanan yang penting oleh masyarakat di sekitar pantai, termasuk di Sungai Donan Cilacap. Namun, habitat kerang totok mengalami pengurangan akibat overeksploitasi, pencemaran perairan dan sebagainya sehingga perlu adanya upaya pelestarian. Hal tersebut mendorong perlu dilakukannya penelitian tentang keanekaragaman genetic kerang totok. Penelitian ini dilakukan untuk pendapatkan primer yang menghasilkan marka RAPD (Randomly Amplified Polymorphic DNA) yang konsisten, memiliki pita polimorfik, dan mendapatkan informasi tentang keanekaragaman genetik kerang totok di Sungai Donan Cilacap. Penelitian ini menggunakan metode survei dengan teknik pengambilan sampel secara incidental sampling. Data yang diamati mencakup karakter molekuler berupa marka RAPD yang diamplifikasi menggunakan 10 primer acak. Genom total diisolasi dari jaringan otot 17 individu kerang totok. Amplifikasi marka RAPD dilakukan menggunakan teknik PCR (Polymerase Chain Reaction). Metode analisis dilakukan secara statistic menggunakan AMOVA (Analysis of Molecular Variances) berdasarkan ada tidaknya pita DNA spesifik pada gel agarosa. Hasil penelitian menunjukkan bahwa delapan dari sepuluh primer terseleksi mampu mengamplifikasi marka RAPD serta menghasilkan pita polimorfik. Kedelapan primer tersebut adalah GEN-11, GEN-13, GEN-14, GEN-15, GEN-16, GEN-20, GEN-23, dan GEN-24. Analisis keanekaragaman genetik menunjukkan bahwa 25 marka RAPD yang dapat diamplifikasi menggunakan kedelapan primer tersebut memiliki nilai polimorfisme sangat tinggi, yaitu sebesar 100%. Besarnya nilai keanegaragaman genetik yang diperoleh adalah 1,000 ± 0,0202. Kata kunci : RAPD, Polymesoda erosa, Sungai Donan Cilacap, Keanekaragaman genetik.
Co-Authors Aruan, Maroloan Cenwira, Wishia Jessica Novia Juandy Jo Karnelasatri, . Lusiana, Diana Intan Gabriella Mardiyono, Maleakhi Samuel Purba, Naomi Damayanti Santoso, Feronia Reni Cyrena Situmorang, Dolito Tahalele, Nava Anabel Yacob, Iren Anggreni