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All Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Sinergitas PkM & CSR J-HESTECH (Journal Of Health Educational Science And Technology) Jurnal Insan Farmasi Indonesia
Andareas, Pangeran
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Biochemistry, Genetics & Molecular Biology Chemistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health Social Sciences

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Deteksi Genetik Sars-Cov-2 di Swab Lingkungan Asrama Mahasiswa Fakultas Keperawatan Aruan, Maroloan; Andareas, Pangeran; Jo, Juandy
Journal of Health Educational Science And Technology Vol. 6 No. 2 (2023): J-HESTECH (Journal Of Health Educational Science And Technology)
Publisher : Faculty of Health Sciences , Dr. Soetomo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25139/htc.v6i2.6682

Abstract

Pemerintah telah menerapkan serangkaian langkah khusus untuk mengendalikan penyebaran virus SARS-CoV-2 di tempat kerja dan lingkungan sosial. Proses pengendalian ini sangat penting, karena ketidakmampuan untuk menemukan carriers/ pembawa sebelum menunjukkan dan tanpa gejala, dapat mengganggu efektivitas proses pengendalian. Penularan SARS-CoV-2 dari pasien bergejala dan tanpa gejala terjadi melalui droplet yang dikeluarkan saat batuk atau bersin tanpa menutup mulut atau hidung. Namun, hal ini tentu tidak menutup kemungkinan terjadinya infeksi melalui kontak sentuhan pada permukaan benda dan lingkungan. Hipotesis fomite ini dianalisis dengan menguji sampel swab lingkungan menggunakan metode PCR kuantitatif Reverse Transcription (RT-qPCR). Dalam penelitian ini, deteksi genetik yang dilakukan di 13 titik di asrama mahasiswa Universitas Pelita Harapan selama bulan November 2021, enam sampel dinyatakan positif, ditunjukkan dengan Cycle threshold (Ct) RT-qPCR yaitu di lantai 15: lift luar kancing (Ct= 36,69), kancing mesin cuci (Ct=37,24), gagang pintu kamar (Ct=36,79), gagang lemari es (Ct=36,6); di lantai 16: tombol lift luar (Ct=37,47) dan pegangan kursi tunggu lift (Ct=37,84).
POTENTIAL OF CITRUS LEAF EXTRACT (Cymbopogon citratus (DC.) Stapf) AS AN ANTIBACTERIAL INHIBITORY of Staphylococcus epidermidis Karnelasatri, Karnelasatri; Cenwira, Wishia; Andareas, Pangeran; Santoso, Feronia Reni Cyrena
Jurnal Insan Farmasi Indonesia Vol 6 No 2 (2023): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v6i2.1601

Abstract

The concerns of antibiotic resistance have increased in recently years, this issue caused by long-term repeated use of antibiotics to treat bacterial illnesses. Lemongrass leaf (Cymbopogon citratus (DC.) Stapf) is one of the natural ingredients that can be used as an alternative antibacterial. The study objective is to determine the antibacterial activity of the ethanol extract of 70% cooking leaves against Staphylococcus epidermidis using diffusion methods. In addition, the Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of extracts were determined using liquid dilution methods. The results of this study show that extract concentrations of 1,000,000 ppm have a barrier zone in the intermediate category when compared with the positive control, that is, clindamycin. Meanwhile, the MIC value obtained is 1250 ppm, while the MBC value is 5000 ppm. The results of statistical analysis showed that there were no significant differences in the inhibition zone diameter formed between the test concentrations.
Antibacterial Activity Assay on Escherichia coli of The Active Fraction Matoa Seeds (Pometia Pinnata J.R Forst & G. Forst.) Karnelasatri, Karnelasatri; Yacob, Iren Anggreni; Andareas, Pangeran; Santoso, Feronia Reni Cyrena; Novia, Jessica
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 10 No. 2 (2024): (October 2024)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2024.v10.i2.16283

Abstract

One of the plants had been used as a medicinal plant in Papua is Pometia Pinnata J.R Forst & G. Forst or the Matoa plant. The aim of this study is to determine the antibacterial activity of the active fraction of Matoa seeds against Escherichia coli bacteria. The positive control and negative control used were ciprofloxacin and dimethyl sulfoxide, respectively. 96% ethanol solvent was used to extract the simplicia of Matoa seeds. Fractionation was carried out using n-hexane, ethyl acetate and water as solvents. The Kirby Bauer diffusion method was used in the antibacterial activity assay at concentrations of 100 mg/mL, 200 mg/mL, 300 mg/mL, 400 mg/mL, and 500 mg/mL. Overall, the inhibition zones obtained from the sequential concentrations above were 8.89 mm, 10.18 mm, 10.34 mm, 10.84 mm, and 11.76 mm respectively. Further, data analysis was performed with One Way ANOVA and the results showed significant differences between concentrations. Data analysis was then continued with the post hoc Tukey HSD test and a concentration of 500 mg/mL was concluded as the optimum concentration.
TRAINING ON BIOCHEMICHAL IDENTIFICATION OF ENTEROBACTERIACEAE USING THE ENTEROSYSTEM 24R KIT TO STUDENTS OF MEDICAL LABORATORY TECHNOLOGY AT YARSI MEDIKA VOCATIONAL HIGH SCHOOL, TANGERANG Andareas, Pangeran; Aruan, Maroloan; Lusiana, Diana Intan Gabriella; Mardiyono, Maleakhi Samuel; Situmorang, Dolito; Purba, Naomi Damayanti; Tahalele, Nava Anabel
Jurnal Sinergitas PKM & CSR Vol. 8 No. 3 (2024): DECEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tri Dharma Perguruan Tinggi is the main foundation for universities in Indonesia in carrying out their functions and responsibilities. One of the activities that is part of the Tri Dharma of Higher Education is Community Service (CS). PkM activities have the aim of improving the welfare of the community by providing solutions to various problems that exist in the community. One solution that can be provided is to provide training to the community with the aim of increasing knowledge, skills or competencies of human resources (HR).  One of the important communities to improve competence is school students, especially vocational high schools (VHS) majoring in medical laboratory technology (MLT). This activity is important so that the graduates produced are in accordance with the needs of the world of work such as hospitals, clinics and research institutions. This activity was given to students majoring in medical laboratory technology, VHS Yarsi Medika Tangerang. The topic raised in this activity is training in the identification of enterobacteriaceae using the Enterosystem 24R kit. The data in this activity was obtained with a Quiz instrument (Pre-Test and Test) using Google Form.  The results obtained from PkM activities show that there is an increase in student knowledge related to PkM material by 35%.
Co-Authors Aruan, Maroloan Cenwira, Wishia Jessica Novia Juandy Jo Karnelasatri, . Lusiana, Diana Intan Gabriella Mardiyono, Maleakhi Samuel Purba, Naomi Damayanti Santoso, Feronia Reni Cyrena Situmorang, Dolito Tahalele, Nava Anabel Yacob, Iren Anggreni