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All Journal Journal of the Indonesian Tropical Animal Agriculture Tropical Animal Science Journal
Fidriyanto, R.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Energy

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Characteristic of different level of fermented concentrate in the rumen metabolism based on in vitro Sari, N. F.; Ridwan, R.; Rohmatussolihat, R.; Fidriyanto, R.; Astuti, W. D.; Widyastuti, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.296-305

Abstract

Digestibility is a description of the ability of feed material that can be utilized by livestock. Higher digestibility of feed material means the total amount of feed content that can be digested in the digestive tract. This study aimed to obtain the proportion of the use of fermentation concentrate in rumen fermentation based on in vitro. The feed used consisted of 7 treatments as follows P1 (20% Pennisetum purpureum: 80% concentrate), P2 (30% P.purpureum : 70% concentrate), P3 (40% P.purpureum : 60% concentrate), P4 (50% P.purpureum : 50% concentrate), P5 (20% concentrate: 80% P.purpureum), P6 (30% concentrate: 70% P.purpureum) and P7 (40% concentrate: 60% P.purpureum). Dry matter digestibility (DMD) and organic matter digestibility (OMD) were done using Theodorou method. Gas production was measured when the samples were incubated at 39oC in the waterbath incubator at hours 2, 4, 6, 8, 10, 12, 24, and 48. Approximately 0.75 g of feed formulation was mixed with 75 mL of rumen fluid buffer. The rumen fluid is taken from 3 fistulated cattles prior to morning feeding. A complete randomized design with three replicates was used. Asymptotic gas production and kinetics were estimated by the Orskov’s model. The results of this study showed that P2 and P3 treatment showed the best digestibility among others treatment with 71.63% ; 71.06% of DMD and 76.42% ; 71.65% of OMD. In conclusion, P2 and P3 treatment can be used as feed for beef cattle. 
In vitro rumen fermentability kinetics of parboiled rice bran Fidriyanto, R.; Ridwan, R.; Rohmatussolihat, R.; Astuti, W. D.; Sari, N. F.; Adi, E. B. M.; Mulyaningsih, E. S.; Widyastuti, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.96-105

Abstract

Parboiled rice product has been widely produced by industry in the developing countries. The increasing of parboiled rice product will consequently increased rice bran waste. Therefore, the objective of the study was to analyze the effect of parboiling processes on nutritional value of rice bran and evaluate nutritional value of parboiled rice bran in the rumen fermentation using in vitro digestibility method. The experiment was arranged in completely randomized factorial design with 2 factors and 3 replications. The first factor is types of rice bran from different rice plant variety consisted of LIPI GO1, LIPI GO2, LIPI GO4, and Sintanur. The second factor is soaking time consist of 0 minute (control), 30 minutes and 60 minutes. Data were analyzed by using ANOVA and significant effects of each treatment were further analyzed by using the least significant difference by Duncan multiple range test (P<0.05). Kinetic parameters of Ørskov’s equation were obtained by non-linear regression procedure. Results showed that the quality of parboiled rice bran was significantly affected by the parboiling process such as soaking periods and rice variety. Chemical composition, rumen gas production, and digestibility of rice bran differ depends on rice variety. Soaking for 30 minutes and 60 minutes decreased organic matter, crude protein, and increased crude fiber (P<0.05). Moreover, soaking rice grain for 60 minutes increased crude fat. The longer of soaking periods decreased of total gas, potential gas production, gas production rate and total organic matter digestibility (P<0.05).
Molecular Identification, Chemical Composition, and In Vitro Anthelmintic Activity of Sargassum duplicatum Against Haemonchus contortus Sakti, A. A.; Kustantinah; Sofyan, A; Nurcahyo, R. W.; Fidriyanto, R.; Kusnadi, H.; Prasetyo, A.; Putnarubun, C.; Permadi, S.; Pramono; Hartati, L.; Hudaifa, I.; Suwignyo, B.
Tropical Animal Science Journal Vol. 47 No. 2 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.2.188

Abstract

The resistance of the Haemonchus contortus, a parasite causing severe anemia in ruminants, to commercial anthelmintics emphasizes the need for alternative bio-anthelmintics. This study aimed to identify the molecular, chemical composition, and in vitro anthelmintic activity of Sargassum duplicatum against H. contortus. Molecular identification employed the Chelex method, with DNA diversity and phylogeny assessed using maximum likelihood in IQ-tree. The analyzed chemical composition included proximate, total flavonoid, and total phenols. Adult worm motility test (AWMT) and egg hatch inhibiting test (EHIT) were conducted at concentrations of 2, 4, and 6 mg/mL of S. duplicatum ethanolic extract. AWMT followed a completely randomized factorial design (5 replications, each with 5 worms), while EHIT used a completely randomized one-way design (5 replications, each with H. contortus egg batches from 3 adult female worms). The cox1 gene sequence revealed the Sargassum sample as S. duplicatum (KP101270.1) with 99.83% similarity. The results indicated that the identified concentrations of S. duplicatum ethanolic extract, and the observation time significantly influenced motility and egg hatchability (p<0.05). Both factors exhibited a significant interaction (p<0.05). Concentrations of 4-6 mg/mL reduced worm motility by up to 50% (LD50) within 6-8 hours, while concentrations of 2-6 mg/mL inhibited egg hatchability by more than 87% during 24 hours of incubation. In conclusion, S. duplicatum holds significant potential as a bio-anthelmintic agent.
Co-Authors A Sofyan A. A. Sakti, A. A. Adi, E. B. M. Astuti, W. D. B. Suwignyo, B. Hartati, L. Hudaifa, I. Kusnadi, H. Kustantinah Mulyaningsih, E. S. Nurcahyo, R. W. Permadi, S. Pramono Prasetyo, A. Putnarubun, C. R. Ridwan Rohmatussolihat, R. Sari, N. F. Y. Widyastuti