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Level of sodium chloride (NaCl) and profile of cervical mucus of dairy cattle at various age synchronized by prostaglandine Ondho, Y. S.; Akbar, F. A.; Lestari, D. A.; Samsudewa, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.364-371

Abstract

Objectives of this study were to compare the percentage of Sodium Chloride (NaCl) and cervical mucus profile which includes potential of Hydrogen (pH), abundance of cervical mucus, spinnbarkeit and ferning of dairy cattle at different age during estrus cycle. Thirty Two head of dairy cattle were used as materials which are 2 years old (n = 2), 3 years old (n = 18), 4 years old (n = 8) and 5 years old (n=4). Dairy cattle were estrus synchronized using 50 mg/head of prostaglandin. Data was collected on 48, 72, 78, 84, 90, 120, 144 and 408 hours after prostaglandin administration. The data were analyzed by using non parametric statistic which was Kruskal-Wallis H test. The significant data was tested with Mann-Whitney U test. The result showed that the abundance of cervical mucus was significantly different (P<0.05; χ2 = 0.011) on 84 hours after estrus synchronization. However, level of NaCl, abundance, spinnbarkeit and ferning of cervical mucus was not sigificantly different. In conclusion, the age of dairy cattle did not affected to the condition NaCl percentage, spinnbarkeit, pH and ferning of cervical mucus, but affected to the abundance of cervical mucus on 84 hours after adminitration of prostaglandine.
Engklek Game Ethnoscience-Based Learning Material (EGEBLM) to Improve Students’ Conceptual Understanding and Learning Motivation Hariyono, E.; Rizki, I. A.; Lestari, D. A.; Citra, N. F.; Islamiyah, A. N.; Agusty, A. I.
Jurnal Pendidikan IPA Indonesia Vol 12, No 4 (2023): December 2023
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v12i4.43941

Abstract

Understanding concepts is essential in physics education, as this subject emphasizes the practical application of concepts over rote memorization. This approach helps prevent students from occurring misconceptions. Moreover, learning motivation can raise enthusiasm for learning physics to achieve the expected goals. However, evidence suggests that both variables show low levels. Therefore, this study aims to develop valid, practical, and effective Engklek game ethnoscience-based edutainment learning materials (EGEBLM) to improve students' physics concept understanding and learning motivation. This research utilizes the 4D type (Define, Design, Develop, Dissemination) to fulfill the quality test of the developed learning materials. The sample of this study involved 29 high school students at level XI. The EGEBLM test design used a non-equivalent control group design. Data were analyzed using descriptive and statistical tests, including N-gain, t-test, and MANOVA. The results reveal that EGEBLM is declared valid in content and construct in each component, including syllabus, lesson plans, teaching materials, student worksheets, test instruments, and response questionnaires. Observations indicate that each learning approach's syntax can be effectively implemented ( 3.00), rendering it suitable for educational applications. EGEBLM also meets effective criteria based on descriptive and statistical tests (p 0.05) to enhance conceptual understanding and learning motivation, receiving positive feedback from students to this learning. This learning model combines collaborative discovery learning activities with traditional Engklek games that they have applied everyday, enabling students to understand physics concepts easily and meaningful learning experience. This research has implications as an innovative method in learning physics edutainment, offering an accessible, cost-effective, enjoyable approach while also preserving the Engklek cultural context. 
Genetic diversity and phylogenetic analysis of Indonesian local and commercial rabbit breeds based on mitochondrial D-loop Lestari, D. A.; Latifa, A. P.; Sutopo, S.; Kurnianto, E.; Setiatin, E. T.; Agustine, A. D.; Nabilah, Z.; Kamila, F. T.; Prahara, P. G.; Kamalludin, M. H.; Setiaji, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 3 (2025): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.3.189-198

Abstract

This study aimed to evaluate the genetic diversity and phylogenetic relationships among four rab-bit breeds raised in Central Java, namely Indonesian Local rabbit (LC), Hycole (HC), Hyla (HL), and New Zealand White (NZ), using mitochondrial D-loop region sequences. A total of 40 blood samples were collected from rabbits in three regencies in Central Java. DNA was extracted from each sample, followed by gene amplification, Sanger sequencing, and sequence analysis. A 906 bp fragment of the mitochondrial D-loop region was successfully amplified from all individuals. The overall genetic di-versity was found to be low, with mean values of k = 4.615, nucleotide diversity (π) = 0.00509 ±0.00092, and haplotype diversity (Hd) = 0.385 ± 0.070. Genetic distance analysis showed a close rela-tionship among the breeds, ranging from 0.00349 to 0.00610. Two haplotypes (A and B) were identi-fied, and both were shared across all breeds, indicating limited genetic differentiation. Phylogenetic tree reconstruction further demonstrated that the four breeds clustered closely together and were most likely derived from European lineages rather than distinct indigenous origins. In conclusion, rabbits raised in Central Java represent a genetically homogenous group with low diversity, highlighting the need for broader genetic resource management and conservation strategies to ensure long-term popula-tion sustainability.