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All Journal Jurnal Pendidikan IPA Indonesia Journal of the Indonesian Tropical Animal Agriculture
Lestari, D. A.
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education

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Level of sodium chloride (NaCl) and profile of cervical mucus of dairy cattle at various age synchronized by prostaglandine Ondho, Y. S.; Akbar, F. A.; Lestari, D. A.; Samsudewa, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.364-371

Abstract

Objectives of this study were to compare the percentage of Sodium Chloride (NaCl) and cervical mucus profile which includes potential of Hydrogen (pH), abundance of cervical mucus, spinnbarkeit and ferning of dairy cattle at different age during estrus cycle. Thirty Two head of dairy cattle were used as materials which are 2 years old (n = 2), 3 years old (n = 18), 4 years old (n = 8) and 5 years old (n=4). Dairy cattle were estrus synchronized using 50 mg/head of prostaglandin. Data was collected on 48, 72, 78, 84, 90, 120, 144 and 408 hours after prostaglandin administration. The data were analyzed by using non parametric statistic which was Kruskal-Wallis H test. The significant data was tested with Mann-Whitney U test. The result showed that the abundance of cervical mucus was significantly different (P<0.05; χ2 = 0.011) on 84 hours after estrus synchronization. However, level of NaCl, abundance, spinnbarkeit and ferning of cervical mucus was not sigificantly different. In conclusion, the age of dairy cattle did not affected to the condition NaCl percentage, spinnbarkeit, pH and ferning of cervical mucus, but affected to the abundance of cervical mucus on 84 hours after adminitration of prostaglandine.
Engklek Game Ethnoscience-Based Learning Material (EGEBLM) to Improve Students’ Conceptual Understanding and Learning Motivation Hariyono, E.; Rizki, I. A.; Lestari, D. A.; Citra, N. F.; Islamiyah, A. N.; Agusty, A. I.
Jurnal Pendidikan IPA Indonesia Vol 12, No 4 (2023): December 2023
Publisher : Program Studi Pendidikan IPA Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpii.v12i4.43941

Abstract

Understanding concepts is essential in physics education, as this subject emphasizes the practical application of concepts over rote memorization. This approach helps prevent students from occurring misconceptions. Moreover, learning motivation can raise enthusiasm for learning physics to achieve the expected goals. However, evidence suggests that both variables show low levels. Therefore, this study aims to develop valid, practical, and effective Engklek game ethnoscience-based edutainment learning materials (EGEBLM) to improve students' physics concept understanding and learning motivation. This research utilizes the 4D type (Define, Design, Develop, Dissemination) to fulfill the quality test of the developed learning materials. The sample of this study involved 29 high school students at level XI. The EGEBLM test design used a non-equivalent control group design. Data were analyzed using descriptive and statistical tests, including N-gain, t-test, and MANOVA. The results reveal that EGEBLM is declared valid in content and construct in each component, including syllabus, lesson plans, teaching materials, student worksheets, test instruments, and response questionnaires. Observations indicate that each learning approach's syntax can be effectively implemented ( 3.00), rendering it suitable for educational applications. EGEBLM also meets effective criteria based on descriptive and statistical tests (p 0.05) to enhance conceptual understanding and learning motivation, receiving positive feedback from students to this learning. This learning model combines collaborative discovery learning activities with traditional Engklek games that they have applied everyday, enabling students to understand physics concepts easily and meaningful learning experience. This research has implications as an innovative method in learning physics edutainment, offering an accessible, cost-effective, enjoyable approach while also preserving the Engklek cultural context. 
Genetic diversity and phylogenetic analysis of Indonesian local and commercial rabbit breeds based on mitochondrial D-loop Lestari, D. A.; Latifa, A. P.; Sutopo, S.; Kurnianto, E.; Setiatin, E. T.; Agustine, A. D.; Nabilah, Z.; Kamila, F. T.; Prahara, P. G.; Kamalludin, M. H.; Setiaji, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 3 (2025): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.3.189-198

Abstract

This study aimed to evaluate the genetic diversity and phylogenetic relationships among four rab-bit breeds raised in Central Java, namely Indonesian Local rabbit (LC), Hycole (HC), Hyla (HL), and New Zealand White (NZ), using mitochondrial D-loop region sequences. A total of 40 blood samples were collected from rabbits in three regencies in Central Java. DNA was extracted from each sample, followed by gene amplification, Sanger sequencing, and sequence analysis. A 906 bp fragment of the mitochondrial D-loop region was successfully amplified from all individuals. The overall genetic di-versity was found to be low, with mean values of k = 4.615, nucleotide diversity (π) = 0.00509 ±0.00092, and haplotype diversity (Hd) = 0.385 ± 0.070. Genetic distance analysis showed a close rela-tionship among the breeds, ranging from 0.00349 to 0.00610. Two haplotypes (A and B) were identi-fied, and both were shared across all breeds, indicating limited genetic differentiation. Phylogenetic tree reconstruction further demonstrated that the four breeds clustered closely together and were most likely derived from European lineages rather than distinct indigenous origins. In conclusion, rabbits raised in Central Java represent a genetically homogenous group with low diversity, highlighting the need for broader genetic resource management and conservation strategies to ensure long-term popula-tion sustainability.
Quantifying of morphological character for Kacang goat using principal component factor analysis Lestari, D. A.; Sutopo, S.; Kurnianto, E.; Dagong, M. I. A.; Bugiwati, S. R. A.; Mamat-Hamidi, K.; Yakubu, A.; Pandupuspitasari, N. S.; Agusetyaningsih, I.; Kamila, F. T.; Setiaji, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 4 (2024): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.4.316-322

Abstract

The study’s objective was to estimate the association among various linear body measurements and body weights of adult Kacang goats. The data was obtained from 209 heads of adult Kacang Goat, compressed 78 bucks and 131 ewes. The morphological evaluation was performed by measuring body weight (BW), body length (BL), chest depth (CD), chest girth (CG), chest width (CW), and withers height (WH). Factor PROCEDURE was performed to estimate the principal component. The result of factor analysis was used to determine the independent variable for linear regression analysis. BW has a favorable correlation with CG, BL, CD, CW, and WH for bucks and ewes. PC 1 accounts for 55.62% of the variation in bucks, while PC 2 accounts for an additional 18.34%. PC 1 accounts for just 0.45% of the overall variation in ewes, whereas PC 2 accounts for 0.24%. The R-squared (R2) values for bucks and ewes in the regression equation with CG as the independent variable are 0.32 and 0.41, respectively. For both bucks and ewes, the regression equation with CW as the independent variable had a higher R2 of 0.52 and 0.20, respectively. For bucks and ewes, the regression equation'sR2 values are 0.54 and 0.44, respectively, with combined CG and CW acting as independent variables. This integrated approach to analyzing body measurements in Kacang Goats provides a robust foundation for making informed decisions in goat farming.
Conditioned media and DMSO enhance the cryopreservation of bovine adipose tissue-derived mesenchymal stem cells Irfan, S.; Suyatno, S.; Zulfiqar, H.; Lestari, D. A.; Hafid, A.; Kostaman, T.; Herdis, H.; Priyatno, T. P.; Sitaresmi, P. I.; Hudaya, M. F.; Lupitasari, F. B. I.; Pangestu, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 2 (2024): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.2.181-190

Abstract

Mesenchymal stem cells derived from adipose tissue (AD-MSCs) show great potential for repro ductive biotechnology in the livestock sector. However, enzyme-based isolation of MSCs is expensive and time-consuming, so it is still rarely done, especially for applications in the livestock sector in de veloping countries. So, MSCs must be cryopreserved with an efficient cryoprotective agent to be stored and reproduced in various laboratories after isolation. This study was aimed to optimize the cryopreser vation media for adipose-derived MSCs in cattle. This study evaluated the viability, proliferation, and morphology of AD-MSCs. The results of this study indicate that a combination of 10% DMSO, 45% DMEM, and 45% conditioned media significantly improves post-thaw viability, proliferation, and sur vival as compared to other mediums. Furthermore, AD-MSCs cryopreserved in this medium exhibit similar morphology as fresh cells. These findings suggest that the optimized cryopreservation medium can enhance the quality and safety of AD-MSCs for clinical applications in the livestock industry.
DNA variants and population structure of Magelang ducks across generation Febriana, A.; Kurnianto, E.; Sutopo, S.; Lestari, D. A.; Setiaji, A.; Sugiharto, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 49, No 1 (2024): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.49.1.67-77

Abstract

Magelang ducks (MD) are Indonesian local ducks and are known as dual-purpose type ducks which have high egg production, duck day production (DDP), and body weight size. Nowadays, the MD is selected to establish the pure line to advance the egg production trait in the Breeding and Rearing Center of Non-Ruminant Animals Banyubiru, Central Java. The mitochondrial DNA (mtDNA) Dloop region, is highly polymorphic and could be used to analyze the population genetics. The present research aims to examine the impacts of continuous selection on population structure and genetic mutations on MD across two generations using the mtDNA D-loop region. Thirty-six blood samples from the second (G2) and third (G3) generations were examined using the sequencing method. The MEGA X and DnaSP software were applied to calculate the genetic diversity, genetic distance, and to generate a phylogenetic tree. The number of haplotypes (H), haplotype diversity (Hd), and Tajima's D are 26, 0.9746, and -1.46, respectively. The Fst value of MD is 0.156. The genetic distance among populations ranges from 0.0000 – 2.097. The UPGMA analysis constructs one clade in a phylogenetic tree between MD, Indonesian local Ducks, Indian Ducks, Vietnamese Ducks, and Chinese Ducks. The study found that the genetic variation and population structure did not significantly change between the second and third generations.
Co-Authors A. Hafid, A. A. Setiaji A. Yakubu Agusetyaningsih, I. Agustine, A. D. Agusty, A. I. Akbar, F. A. Bugiwati, S. R. A. Citra, N. F. D. Samsudewa E. Kurnianto E. T. Setiatin, E. T. Febriana, A. Hariyono, E. Herdis, H. Hudaya, M. F. Irfan, S. Islamiyah, A. N. Kamalludin, M. H. Kamila, F. T. KOSTAMAN, T. Latifa, A. P. Lupitasari, F. B. I. M. I. A. Dagong, M. I. A. M. Pangestu Mamat-Hamidi, K. Nabilah, Z. Ondho, Y. S. Pandupuspitasari, N. S. Prahara, P. G. Rizki, I. A. S. Sugiharto Sitaresmi, P. I. Sutopo Sutopo Suyatno, S. T. P. Priyatno, T. P. Zulfiqar, H.