E. T. Setiatin, E. T.
Faculty of Animal and Agricultural Sciences, Diponegoro University, Tembalang Campus, Semarang 50275, Central Java

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Tampilan Ferning Pre - Post Inseminasi Buatan Berdasarkan Umur Sapi Simpo F1 Dan F2 Di Kecamatan Patean, Kabupaten Kendal, Jawa Tengah A., Andini, N.; Setiatin, E. T.; Sutopo, Sutopo
Jurnal Pengembangan Penyuluhan Pertanian Vol 13, No 23 (2016): Juli
Publisher : UPPM Politekik Pembangunan Pertanian Yogyakarta Magelang (Polbangtan Yoma)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1150.088 KB) | DOI: 10.36626/jppp.v13i23.105

Abstract

Penelitian ini bertujuan untuk mengetahui tampilan ferning sapi betina SIMPO F1 dan F2 berdasarkan umur di Kecamatan Patean, Kabupaten Kendal, Jawa Tengah. Materi yang digunakan dalam penelitian adalah 20 ekor sapi betina SIMPO, masing-masing 7 ekor SIMPO F1 dan 13 ekor SIMPO F2 milik peternak rakyat. Metode pengambilan lendir serviks menggunakan ulas vagina, dikoleksi sebanyak 6 jam sekali dalam 24 jam, dengan menggunakan cotton bud. Parameter yang diamati meliputi tampilan ferning, kelimpahan dan konsistensi lendir serviks. Gambaran ferning diperoleh dengan cara mengoleskan lendir serviks di atas object glass, dikeringudarakan kemudian diamati dengan bantuan mikroskop dengan perbesaran 10x10. Gambaran tampilan ferning sapi betina SIMPO F1 dan F2 bervariasi, berupa cabang primer, sekunder dan tersien. Kemudian dievaluasi berdasarkan skoring. Gambaran terlihat lebih jelas dan nyata membentuk daun pakis. Hasil uji observasional dan deskriptif dapat disimpulkan bahwa tampilan ferning, kelimpahan dan konsistensi berbeda pada 20 SIMPO F1 dan F2. 
LEVEL OF ESTROGEN HORMONE AND ESTRUS PERFORMANCE OF DIFFERENT POSTPARTUM ESTRUS OF JAWA RANDU GOAT Tanjung, A. D.; Setiatin, E. T.; Samsudewa, D.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 2 (2015): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.2.87-92

Abstract

The purpose of this research was to determine the reproductive quality of normal compared withabnormal postpartum estrus (PPE) of Jawa Randu Goats (n = 16 goats) on estrogen hormon level andestrus profile. Total samples were 16 Jawa Randu goats. The data were analyzed descriptively and nonparametric data were analyzed using Mann Whitney U-test of Statistical Package for the Social science(SPSS) 16. Descriptive statistics was used on estrogen hormone and resulting that normal postpartumestrus (PPE) Jawa Randu Goats had a better level (133.8; 141.5; 155.6; 162.25; 167.75 pg/mL)compared with abnormal postpartum estrus of Jawa Randu Goat (109.9; 111.35; 101.2; 132.2; 142.45pg/mL). Mann Whitney U-test used for the abundance of cervical mucus and ferning showed nonsignificantly different of postpartum estrus between both groups. Jawa Randu goats with normal PPEhad a higher estrogen level than abnormal PPE goats. However, for the abundance and ferning ofcervical mucus, between the two groups of goats was not different.
Leucocyte profile and offspring production of guinea pig (Cavia cobaya) given Anredera cordifolia leaf extract Wijayanti, D.; Setiatin, E. T.; Kurnianto, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 1 (2018): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.1.19-25

Abstract

The objective of this study was to determine leucocyte and offspring production of guinea pig (Cavia cobaya) giving Anredera cordifolia leaf extract. Materials used were female 16 heads of guinea pig with body weight of 425g. The treatments were an extract of A. cordifolia leaf at doses of 0, 10, 50 and 90 mg/head, designated as T0, T1, T2 and T3, respectively. A. cordifolia leaf extract was administered orally from 10 days prepartum to 10 days postpartum. Blood was taken at 10 days prepartum and 10 days postpartum. Total birth of the offspring was observed. Data were analyzed by analysis of variance and if there was effect of treatment, then continued with Duncan multiple range test and Chi-Square test for fetal production between the given A. cordifolia leaf extract and control. The result showed that there was no significant difference for 10 days prepartum after addition of A cordifolia leaf extract treatment. The postpartum treated showed a total 50 mg/head level increaed for monocytes than that of level 0, 10 and 90 mg/head. Ten days postpartum treatment showed the total increase for leucocyte and monocytes total were 50 and 90 mg/head, respectively compared to 10 mg/head level. Total lymphocyte of 90 mg/head increased compared to level 10 and 50 mg/head. The highest total neutrophil as found at level of 50 mg/head which increased compared to the level of 0 and 10 mg/head. ProvisioningA. cordifolialeaf extract at doses level of 50 and 90 mg/head could increase litter size (P<0.05; χ2=9.267) and decreased offspring mortality (P<0.05; χ2=6.4). In conclusion, by giving 50 mg/head A. cordifolia leaf extract could increase leucocyte profile and offspring production of guinea pig. 
Genetic diversity and phylogenetic analysis of Indonesian local and commercial rabbit breeds based on mitochondrial D-loop Lestari, D. A.; Latifa, A. P.; Sutopo, S.; Kurnianto, E.; Setiatin, E. T.; Agustine, A. D.; Nabilah, Z.; Kamila, F. T.; Prahara, P. G.; Kamalludin, M. H.; Setiaji, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 50, No 3 (2025): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.50.3.189-198

Abstract

This study aimed to evaluate the genetic diversity and phylogenetic relationships among four rab-bit breeds raised in Central Java, namely Indonesian Local rabbit (LC), Hycole (HC), Hyla (HL), and New Zealand White (NZ), using mitochondrial D-loop region sequences. A total of 40 blood samples were collected from rabbits in three regencies in Central Java. DNA was extracted from each sample, followed by gene amplification, Sanger sequencing, and sequence analysis. A 906 bp fragment of the mitochondrial D-loop region was successfully amplified from all individuals. The overall genetic di-versity was found to be low, with mean values of k = 4.615, nucleotide diversity (π) = 0.00509 ±0.00092, and haplotype diversity (Hd) = 0.385 ± 0.070. Genetic distance analysis showed a close rela-tionship among the breeds, ranging from 0.00349 to 0.00610. Two haplotypes (A and B) were identi-fied, and both were shared across all breeds, indicating limited genetic differentiation. Phylogenetic tree reconstruction further demonstrated that the four breeds clustered closely together and were most likely derived from European lineages rather than distinct indigenous origins. In conclusion, rabbits raised in Central Java represent a genetically homogenous group with low diversity, highlighting the need for broader genetic resource management and conservation strategies to ensure long-term popula-tion sustainability.