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All Journal Jurnal Biodjati Jurnal Biologi Tropis Journal of Halal Science and Research Journal of Biotechnology and Natural Science
Solihah, Jumailatus
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Other

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The Potential of Salvinia molesta as a Copper Phytoremediation Agent based on Gene Expression Analysis Astuti, Sutan Nur Chamida Tri; Solihah, Jumailatus; Aisah, Siti
Journal of Biotechnology and Natural Science Vol. 3 No. 1 (2023): June
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jbns.v3i1.9739

Abstract

This research explores the rapid environmental impact of the batik industry, particularly concerning batik waste pollution, especially from the heavy metal copper (Cu). Untreated batik waste can have adverse effects on the environment. The study highlights the potential of the water fern plant (Salvinia molesta) as a heavy metal phytoremediator, specifically for Cu, by activating phytochelatin synthase. The research aims to determine the most effective waste concentration for Cu absorption by water ferns, measure the reduction in Cu levels after phytoremediation treatment, evaluate post-phytoremediation water quality, and observe PCS gene expression in the roots and leaves of water ferns. A static method is employed with variations in waste concentrations. Data analysis utilizes one-way ANOVA for Cu level reduction and changes in water quality. The results indicate that the most effective wastewater concentration for absorbing heavy metal copper (Cu) using water fern (Salvinia molesta) is 2%. The highest reduction in heavy metal copper (Cu) concentration after phytoremediation treatment with water fern (Salvinia molesta) is 41.48%. Water quality post-phytoremediation treatment using water fern (Salvinia molesta) at all concentrations exhibits improvement with an increase in dissolved oxygen (DO) levels. The expression of the phytochelatin synthase (PCS) gene in the leaves and roots of water fern (Salvinia molesta) confirms the role of the PCS gene in binding heavy metal copper (Cu) to the plant's vacuole.
Glyphosate Tolerant Bacteria from Rhizosphere of Kangkong (Ipomoea reptans Poir.) and Soybean (Glycine max L.) Dina, Aslikh Lana; Solihah, Jumailatus; Khusnuryani, Arifah
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8504

Abstract

The use of organophosphate pesticides has some risks for human health and environment. One of the organophosphate pesticides is glyphosate. Various methods used to detoxify organophosphates including chemical methods, incineration, and landfills, produce acid and alkaline compounds, leaching pesticides around land and groundwater areas, as well as toxic emissions to the environment. The bacteria with this ability can be isolated from areas contaminated with glyphosate. Kangkong (Ipomoea reptans) and soybean (Glycine max) were chosen because of these plants are commonly found in rice fields which are areas that are frequent exposure to pesticide. The interaction between rhizosphere bacteria and plants as well as the composition of existing bacteria are closely related to the remediation occured. Kangkong and soybeans (2 weeks) were treated with glyphosate 377 mM. Soil pH was measured in third and seventh days after treat with glyphosate. The bacteria were isolated a week after treatment with glyphosate, and cultured in NA medium containing 5 mM and 10 mM glyphosate. The growing bacteria were selected and re-cultured in NA + glyphosate 10 mM medium. The selected isolates were tested for glyphosate degradation ability in Mineral Salt Media containing glyphosate 5 mM and glucose 50 mg/L. Eight isolates of bacteria grew in media containing glyphosate, i.e. Kd1, Kd2, Kd3, Kd4, Kd5 from soybeans rhizosphere, and K1, K3, K4 from spinach rhizosphere. The isolate Kd4 and K4 grew more abundantly compared the other isolates, exhibited good tolerant of glyphosate. From glyphosate degrading test, the isolate from soybean rhizosphere showed more tolerance than the isolate from kangkong rhizosphere. The molecular identification revealed that both isolates belong to species Bacillus mycoides.
In-silico primer designing and validation of Bos taurus and Sus scrofa SET proto-oncogene for non-halal detection Muhammadi, Fatimah Mustafawi; Solihah, Jumailatus; Hamdani, Anti Damayanti
Journal of Halal Science and Research Vol. 6 No. 1 (2025): February
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v6i1.11569

Abstract

The SET gene is part of the proto-oncogene family. Proto-oncogenes families are expressed in cell growth regulation, notably during embryogenesis, wound healing, liver regeneration, and mitotic stimulation by growth factors. While proto-oncogenes have been extensively studied for their role in cancer detection, their application in halal certification assessments remains minimal. Polymer Chain Reaction (PCR) has recently been an alternative method in species authentication due to its high accuracy, with primers being a critical component in determining the amplification process's success. Consequently, primers must be carefully and specifically designed. This study uses a bioinformatics approach to explore the potential of the SET proto-oncogene as a candidate primer for non-halal detection. SET proto-oncogene sequences from pig (Sus scrofa) and cattle (Bos taurus) were retrieved from the National Center for Biotechnology Information (NCBI) database and aligned using MEGA6 software to identify conserved regions suitable for primer design. The designed primers were tested through in-silico PCR and subsequently validated using conventional PCR. The results demonstrated that two primer pairs successfully produced amplicons with lengths of 1068 bp and 1231 bp in cattle and 1275 bp and 1222 bp in pigs, consistent with in-silico predictions.   Keywords: Halal, In-silico, Primer, Proto-oncogene, SET gene.
Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers Solihah, Jumailatus; Kurniatanty, Isma; Subositi, Dyah; Maruzy, Anshary; Martiwi, Ika Nugraheny Ari; Ainy, Erny Qurrotul; Anam, Khoirul; Dina, Aslikh Lana
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.14553

Abstract

Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplified Polymorphism (SRAP) Markers. The genomic DNA were extracted from leaf samples and then were characterized by using the SRAP marker system according to Li and Quiros (2001). Nine pairs of SRAP primer resulted high polymorphic bands and were used in the genetic profiling. The data analysis was performed using GenAlEx to calculate genetic distance, Principal coordinate analysis, and Analysis of Molecular Variance (AMOVA), also using POPGENE to assess genetic diversity (Hs and Ht) and Nm to predict gene flow among populations. The coordinate analysis showed that the accessions originated from ethnicities along Wallacean line tend to differ genetically from most other locations. However, the results of analysis of molecular variance suggested that there were only slight differences (0.1%) found between ethnicities, while most genetic variances (99.9%) were found mostly among accessions within populations. The results suggested that there was an extensive genetic flow and plant spreading among Sida rhombifolia plant populations, resulting more homogenous genetic characters among most populations, while high diversity within population. The calculation of the number of migration (Nm = 1.7341) confirmed that the high rate of gene flow had occurred between populations.
Co-Authors Ainy, Erny Qurrotul Arifah Khusnuryani, Arifah Astuti, Sutan Nur Chamida Tri Dina, Aslikh Lana Dyah Subositi Hamdani, Anti Damayanti KHOIRUL ANAM Kurniatanty, Isma Martiwi, Ika Nugraheny Ari Maruzy, Anshary Muhammadi, Fatimah Mustafawi Siti Aisah