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All Journal Jurnal Biologi Tropis Journal of Community Empowerment for Health
Arifah Khusnuryani, Arifah
1Biotechnology Study Program, Universitas Gadjah Mada, Yogyakarta, Indonesia 2Biologi Study Program, UIN Sunan Kalijaga, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Glyphosate Tolerant Bacteria from Rhizosphere of Kangkong (Ipomoea reptans Poir.) and Soybean (Glycine max L.) Dina, Aslikh Lana; Solihah, Jumailatus; Khusnuryani, Arifah
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8504

Abstract

The use of organophosphate pesticides has some risks for human health and environment. One of the organophosphate pesticides is glyphosate. Various methods used to detoxify organophosphates including chemical methods, incineration, and landfills, produce acid and alkaline compounds, leaching pesticides around land and groundwater areas, as well as toxic emissions to the environment. The bacteria with this ability can be isolated from areas contaminated with glyphosate. Kangkong (Ipomoea reptans) and soybean (Glycine max) were chosen because of these plants are commonly found in rice fields which are areas that are frequent exposure to pesticide. The interaction between rhizosphere bacteria and plants as well as the composition of existing bacteria are closely related to the remediation occured. Kangkong and soybeans (2 weeks) were treated with glyphosate 377 mM. Soil pH was measured in third and seventh days after treat with glyphosate. The bacteria were isolated a week after treatment with glyphosate, and cultured in NA medium containing 5 mM and 10 mM glyphosate. The growing bacteria were selected and re-cultured in NA + glyphosate 10 mM medium. The selected isolates were tested for glyphosate degradation ability in Mineral Salt Media containing glyphosate 5 mM and glucose 50 mg/L. Eight isolates of bacteria grew in media containing glyphosate, i.e. Kd1, Kd2, Kd3, Kd4, Kd5 from soybeans rhizosphere, and K1, K3, K4 from spinach rhizosphere. The isolate Kd4 and K4 grew more abundantly compared the other isolates, exhibited good tolerant of glyphosate. From glyphosate degrading test, the isolate from soybean rhizosphere showed more tolerance than the isolate from kangkong rhizosphere. The molecular identification revealed that both isolates belong to species Bacillus mycoides.
Academic community perception of smoke-free zone implementation on campus: situation analysis at UIN Sunan Kalijaga Yogyakarta Warsito, Gita Miranda; Fatimah, Siti; Raharja, Nia Maharani; Khusnuryani, Arifah
Journal of Community Empowerment for Health Vol 8, No 3 (2025)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jcoemph.108566

Abstract

The Indonesian government regulates the use of tobacco products by establishing Smoke-Free Zones (SFZs), including through the Sleman Regent Regulation No. 42/2012, which mandates educational institutions to implement SFZ policies. State Islamic University (UIN) Sunan Kalijaga, located in Sleman Regency, Yogyakarta, is expected to comply with this regulation, especially given its Strategic Plan to establish a Health Faculty and its broader commitment to creating a healthy campus. However, smoking remains prevalent on campus. This study aimed to analyze the perceptions, attitudes, and behaviors of the university’s academic community toward the SFZ policy as a basis for policy development. Data were collected from 1,228 respondents, consisting of 1,164 students and 64 staff (including lecturers), using a self-administered questionnaire. The survey was distributed in January 2024 and promoted through official channels and peer networks to increase the response rate. The data were analyzed using a descriptive approach, with results presented in frequencies and percentages. Findings show that, in general, respondents expressed positive support for the adoption of a campus-wide smoke-free or comprehensive tobacco-free policy.
Co-Authors Dina, Aslikh Lana Nia Maharani Raharja Siti Fatimah Solihah, Jumailatus Warsito, Gita Miranda