Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Indonesian Journal of Biotechnology Journal of Halal Science and Research
Hamdani, Anti Damayanti
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Other

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
A robust in planta Agrobacterium‐mediated transformation in red chili (Capsicum annuum L.) Hamdani, Anti Damayanti; Nugroho, Syarul; Esyanti, Rizkita Rachmi; Faizal, Ahmad; Suhandono, Sony
Indonesian Journal of Biotechnology Vol 29, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.94653

Abstract

Plant improvement through in vitro culture and genetic engineering is a significant aspect of breeding programs aimed at producing disease‐resistant cultivars of disease‐prone red chili (Capsicum annuum L.). However, the Capsicum genus is recalcitrant to genetic transformation and in vitro regeneration. Moreover, developing a universal transformation protocol is difficult due to its highly genotype‐dependent nature. Therefore, this study aimed to develop an Agrobacterium‐mediated in planta transformation method applicable to various red chili cultivars. Two open‐pollinated varieties, Tanjung 2 and Ciko, were subjected to transformation. The young seedlings were immersed in transformation medium containing Agrobacterium tumefaciens strain GV3101 harboring the binary vector pCAMBIA1301, which carries the β‐glucuronidase (GUS) gene. GUS histochemical analysis revealed that all the primary transformants of Tanjung 2 and Ciko were identified as chimeric. The average staining in the body of the seedlings was 88.63 + 26.33% in Tanjung 2, and 90.65 + 16.77% in the Ciko variety. More than 50% of the seedlings continued to express GUS in their shoot areas 10 days after Agrobacterium infection, indicating the possibility of transgene inheritance in the following generation. The in planta transformation approach is notably genotype independent, making it a promising standard transformation protocol for different red chili varieties.
In-silico primer designing and validation of Bos taurus and Sus scrofa SET proto-oncogene for non-halal detection Muhammadi, Fatimah Mustafawi; Solihah, Jumailatus; Hamdani, Anti Damayanti
Journal of Halal Science and Research Vol. 6 No. 1 (2025): February
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jhsr.v6i1.11569

Abstract

The SET gene is part of the proto-oncogene family. Proto-oncogenes families are expressed in cell growth regulation, notably during embryogenesis, wound healing, liver regeneration, and mitotic stimulation by growth factors. While proto-oncogenes have been extensively studied for their role in cancer detection, their application in halal certification assessments remains minimal. Polymer Chain Reaction (PCR) has recently been an alternative method in species authentication due to its high accuracy, with primers being a critical component in determining the amplification process's success. Consequently, primers must be carefully and specifically designed. This study uses a bioinformatics approach to explore the potential of the SET proto-oncogene as a candidate primer for non-halal detection. SET proto-oncogene sequences from pig (Sus scrofa) and cattle (Bos taurus) were retrieved from the National Center for Biotechnology Information (NCBI) database and aligned using MEGA6 software to identify conserved regions suitable for primer design. The designed primers were tested through in-silico PCR and subsequently validated using conventional PCR. The results demonstrated that two primer pairs successfully produced amplicons with lengths of 1068 bp and 1231 bp in cattle and 1275 bp and 1222 bp in pigs, consistent with in-silico predictions.   Keywords: Halal, In-silico, Primer, Proto-oncogene, SET gene.
Co-Authors Ahmad Faizal Muhammadi, Fatimah Mustafawi Nugroho, Syarul RIZKITA RACHMI ESYANTI Solihah, Jumailatus SONY SUHANDONO