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All Journal Jambura Journal of Chemistry Health Sciences and Pharmacy Journal Acta Holistica Pharmaciana
Esati, Ni Ketut
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Analisis Mutu Minyak Zaitun Yang Diperoleh Dari Buah Zaitun (Olea eurofaea) Dan Aplikasinya Sebagai Antikolesterol Duwi Cahyadi, Kadek; Dewi Lestari, Gusti Ayu; Esati, Ni Ketut
Jambura Journal of Chemistry Vol 5, No 1 (2023): February
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34312/jambchem.v5i1.16767

Abstract

Olive oil is oil obtained from olives (Olea europaea L.). Olive oil contains high in oleic acid so it can be used as an anticholesterol. This study aims to determine the quality of olive oil according to SNI 01-4474-1998 and its anticholesterol activity by UV-Vis spectrophotometry. In this research, olive oil quality test was conducted, namely water content test using the thermogravimetric method, fatty acid content test using an alkalimetric titration method based on an acid-base reaction, saponification test using the acidimetric method, and iodine number test using the iodometric titration method. The method of testing the anticholesterol activity of olive oil was carried out in vitro using Lieberman-Burchard reagent. The results of the quality test on olive oil obtained water content of 0.03%, free fatty acids 1.73%, saponification number 190.90 mgKOH/g, iodine number 91.25g iodine/100g, where these results have met the SNI 01-4474-1998 standard. The results of the anticholesterol test showed a decrease in free cholesterol levels by 39.76%. Olive oil shows anticholesterol activity due to the presence of oleic acid or MUFA (Mono Unsaturated Fatty Acids)
MANFAAT SUPLEMEN DALAM MENINGKATAN DAYA TAHAN TUBUH SEBAGAI UPAYA PENCEGAHAN COVID-19 Yani, Ketut Tia Pran Anggar; Kurnianta, Putu Dian Marani; Cahyadi, Kadek Duwi; Esati, Ni Ketut; Sawiji, Repining Tiyas; Darmawan, Gede Agus; Pramana, I Gede Komang Aditya; Sujayanti, Luh Gede Tina; Putra, Kadek Ria Dwitya; Purnamasari, I Gusti Ayu Putu Prima
Acta Holistica Pharmaciana Vol 3 No 1 (2021): Acta Holistica Pharmaciana
Publisher : School of Pharmacy Mahaganesha (Sekolah Tinggi Farmasi Mahaganesha)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62857/ahp.v3i1.33

Abstract

Penyakit coronavirus 2019 (COVID-19) yang bermula di Wuhan, Cina pada akhir tahun 2019 telah menyebar pesat sebagai pandemi global hingga ke Indonesia. Tingginya angka kejadian dan dampak mortalitas yang ditimbulkan mendorong dilakukannya berbagai upaya penanganan maupun pencegahan sesuai karakteristik perjalanan penyakit COVID-19. Salah satu upaya pencegahan infeksi maupun perburukan kondisi penderita COVID-19 adalah melalui penggunaan suplemen untuk peningkatan daya tahan tubuh. Diantara berbagai produk suplemen komersil di pasaran, beberapa komponen mikronutrien seperti vitamin B, C, D dan E, seng, dan selenium serta probiotik adalah komponen penting yang memberikan keuntungan dalam mencegah maupun menghadapi infeksi COVID-19. Bukti-bukti ilmiah melalui penelitian telah menunjukkan bahwa masing-masing kandungan suplemen tersebut berperan dalam peningkatan respon imun dan penyeimbang regulasi proses inflamasi dalam tubuh. Dengan demikian, aplikasi penggunaan suplemen adalah upaya rasional untuk menghadapi COVID-19 menuju era new normal, selama suplementasi sesuai dengan kebutuhan masing-masing individu.
Antibacterial activity extract fraction of black garlic against bacteria propionibacterium acnes with disc diffusion method Pandi, Rensiana Kartini; Jawa La, Elisabeth Oriana; Esati, Ni Ketut
Health Sciences and Pharmacy Journal Vol. 6 No. 3 (2022)
Publisher : STIKes Surya Global Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32504/hspj.v6i3.732

Abstract

Black garlic is the result of processing garlic through a heating process at a temperature of 70oC with a relative humidity of 70-80% for 21 days without additional treatment so that the water content decreases. Black garlic has antibacterial activity due to the presence of bioactive compounds from the phenolic and flavonoid groups. This study aims to determine the secondary metabolite content and antibacterial activity of the black garlic extract fraction against Propionibacterium acnes bacteria. Extraction of Black garlic by maceration method used 96% ethanol as solvent and to obtain extract fraction of Black garlic by multilevel fractionation method using n-hexane, ethyl acetate, and ethanol as solvents. To determine the content of secondary metabolites, phytochemical screening was carried out in each fraction. Antibacterial activity test of Propionibacerium acnes used disc diffusion method with various concentrations of n-hexane, ethyl acetate, ethanol fractions 2%, 4%, 6%, 8%, 10%, positive control (clindamycin 1%), negative control and media control. Data analysis was carried out with qualitative, quantitative, and statistical analysis. The results obtained were the extract fraction of black garlic contains secondary metabolites of flavonoids, alkaloids, saponins, and tannins. The results of the antibacterial activity test showed that the garlic extract fraction had antibacterial activity seen from the presence of an inhibition zone. The largest inhibition zone in the n-hexane fraction of 10% concentration of 8.50 ± 0.10 mm was included in the medium category. The positive control was 12.02 ± 1.41 mm and the negative control gave no inhibition zone.
Co-Authors Darmawan, Gede Agus Duwi Cahyadi, Kadek Gusti Ayu Dewi Lestari Jawa La, Elisabeth Oriana Kadek Duwi Cahyadi Pandi, Rensiana Kartini Pramana, I Gede Komang Aditya Purnamasari, I Gusti Ayu Putu Prima Putra, Kadek Ria Dwitya Putu Dian Marani Kurnianta Sawiji, Repining Tiyas Sujayanti, Luh Gede Tina Yani, Ketut Tia Pran Anggar