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Pengaruh Formulasi Terhadap Mutu Fisik Body Butter Ekstrak Etanol Kulit Buah Naga (Hylocereus polyrhizus) Sawiji, Repining Tiyas; Jawa La, Elisabeth Oriana; Yuliawati, Agustina Nila
Indonesian Journal of Pharmacy and Natural Product Vol. 3 No. 1 (2020)
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.898 KB) | DOI: 10.35473/ijpnp.v3i1.501

Abstract

Kulit buah naga memiliki beragam manfaat walaupun sering dianggap sebagai limbah. Kulit buah naga mengandung banyak pigmen warna, salah satunya antosianin yang berfungsi sebagai antioksidan. Kandungan ini mampu mencegah radikal bebas yang menyebabkan penuaan dini maupun penyakit lainnya. Antioksidan yang terdapat didalam kulit buah naga dapat dimanfaatkan sebagai zat aktif sediaan kosmetika tubuh salah satunya body butter. Salah satu faktor yang mempengaruhi stabilitas sediaan body butter adalah emulgator. Penelitian ini bertujuan untuk mengetahui pengaruh formulasi, yaitu adanya variasi konsentrasi kadar emulgator (tween 60 : span 60) terhadap stabilitas fisik body butter. Sediaan dibuat dengan 3 macam konsentrasi emulgator tween 60 : span 60 yaitu 1%, 2%, dan 3%. Ketiga formula diuji stabilitas fisiknya dengan uji organoleptis, homogenitas, pH, daya lekat, daya sebar, dan daya proteksi. Uji stabilitas fisik dilakukan pada minggu I, II, III, dan IV di suhu ruang. Data pengamatan dianalisis statistik dengan taraf kepercayaan 95%. Hasil evaluasi sediaan body butter menunjukkan bahwa variasi emulgator tidak memberikan pengaruh tehadap sifat fisik body butter meliputi organoleptis, homogenitas, pH dan daya sebar. Namun mempengaruhi uji daya lekat dan daya proteksi (p>0.05). Berdasarkan hasil pengujian, didapatkan hasil yaitu formula III lebih stabil dalam penyimpanan selama 4 minggu daripada formula I dan II.Kata kunci : ekstrak kulit buah naga, emulgator, body butter, stabilitas fisikDragon fruit peels has a variety of benefits although it is often considered a waste. Dragon fruit peels contains many color pigments, one of which is anthocyanin which functions as an antioxidant. This content is able to prevent free radicals that cause premature aging and other diseases. Antioxidants found in dragon fruit peels can be used as active ingredients for cosmetics, one of which is body butter. One of the factors that influence the stability of the body butter preparation is an emulgator. This study aims to determine the effect of the formulation, namely the variation in the concentration of emulgator levels (tween 60: span 60) on the physical stability of body butter. The preparations were made with 3 kinds of 60: span 60 emulsifier concentrations of 1%, 2%, and 3%. The three formulas were tested for physical stability by organoleptic, homogeneity, pH, adhesion, dispersion, and protection. Physical stability tests were carried out at weeks I, II, III, and IV at room temperature. Observation data were analyzed statistically with a confidence level of 95%. The results of the evaluation of body butter preparations showed that variations in the emulgator had no effect on the physical properties of the body butter including organoleptic, homogeneity, pH, and dispersion. But it affects the test of adhesion and protection (p> 0.05). Based on the test results, the results obtained are formula III is more stable in storage for 4 weeks than formulas I and II.Keywords: Hylocereus polyrhizus, emulgators, body butter, physical evaluation
Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus polyrhizus) Jawa La, Elisabeth Oriana; Sawiji, Repining Tiyas; Yuliawati, Agustina Nila
Indonesian Journal of Pharmacy and Natural Product Vol. 3 No. 1 (2020)
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.572 KB) | DOI: 10.35473/ijpnp.v3i1.503

Abstract

Buah naga merah (Hylocereus polyrhizus) merupakan tumbuhan yang sudah banyak dimanfaatkan oleh masyarakat Indonesia secara alami sebagai tanaman kaya antioksidan, sebaliknya pemanfaatan dari kulit buah naga merah belum sepenuhnya dimaksimalkan. Beberapa penelitian yang telah dilakukan menunjukkan bahwa selain daging buah, kulit buah naga merah dapat dimanfaatkan sebagai sumber untuk pengobatan karena kaya akan antioksidan. Hal tersebut dapat digunakan sebagai dasar dan pilihan untuk menghasilkan produk obat tradisional dari kulit buah naga merah. Penelitian ini bertujuan untuk mengidentifikasi kandungan metabolit sekunder yang terdapat pada kulit buah naga merah berupa skrining fitokimia dan kromatografi lapis tipis (KLT) pada senyawa aktif yang disinyalir terkandung didalam kulit buah naga merah yang dapat dimanfaatkan dalam produk kefarmasian. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96% diperoleh rendemen ekstrak sebanyak 2,96%. Skrining fitokimia dilakukan sebagai uji pendahuluan untuk mengidentifikasi kandungan metabolit sekunder yang terkandung dalam ekstrak kulit buah naga merah, dilanjutkan dengan KLT untuk mempertegas hasil reaksi positif. Hasil skrining dan KLT diperoleh kulit buah naga merah positif mengandung alkaloid, tanin, flavonoid, steroid dan potensial untuk dijadikan obat tradisional.Kata kunci :kulit buah naga merah, skrining fitokimia, KLT, metabolit sekunder Red dragon fruits (Hylocereus polyrhizus) generally has been widely known Indonesian as a plant that rich in antioxidant  however, the Red dragon fruits peel is not fully utilized. Some of studies have been conducted indicating that as well as its fruit flesh, Red dragon fruits peel can be beneficial as a source for treatment and traditional medicinal products since it is also rich of antioxidants.This research aims to identify the content of secondary metabolites on the Red dragon fruits peel  in the form of phytochemical screening and thin-layer chromatography (TLC) in the active compounds which can be utilized in the product of  Pharmacy. Extraction is conducted by maceration using the 96% ethanol solvent in order to produces extracts as much as 2.96 %.  Phytochemical screening is delivered as a preliminary test to identify the content of secondary metabolites contained in the peel of  Red dragon fruit peel and continued with the TLC to confirmed give positive  results. Results of  the screening and TLC obtained that the peel of  Red dragon fruits positive contains alkaloids, tannins, flavonoids, steroids and potential to be used as traditional medicineKeyword : Red dragon fruit peel, phytochemical screening, TLC, secondary metabolites 
KAJIAN SENYAWA AKTIF DAN KEAMANAN TANAMAN OBAT TRADISIONAL DI INDONESIA SEBAGAI ALTERNATIF PENGOBATAN MALARIA Jawa La, Elisabeth Oriana; Kurnianta, Putu Dian Marani
Acta Holistica Pharmaciana Vol 1 No 1 (2019): Acta Holistica Pharmaciana
Publisher : School of Pharmacy Mahaganesha (Sekolah Tinggi Farmasi Mahaganesha)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62857/ahp.v1i1.8

Abstract

Malaria merupakan salah satu penyakit menular dengan tingkat prevalensi yang cukup tinggi di Indonesia. Penyakit malaria menjadi semakin serius karena meningkatnya jumlah parasit malaria (Plasmodium) yang resisten terhadap obat-obat antimalaria. Banyaknya keanekaragaman hayati yang ada di Indonesia memotivasi banyaknya penelitian dan pencarian bahan obat baru untuk berbagai terapi, terutama untuk penyakit malaria. Pemanfaatan tanaman obat sebagai agent antimalaria sudah banyak dilakukan dan dikembangkan. Berdasarkan hasil penelitian, banyak senyawa aktif terkandung dalam tanaman yang diklaim sebagai tanaman antimalaria, baik berupa tanaman utuh, simplisia, maupun ekstrak, dan senyawa isolasi. Artikel review ini menggunakan metode studi literatur dari berbagai referensi tentang pemanfaatan dan penggunaan obat tradisional. Beberapa tanaman obat telah diteliti memiliki efek farmakologi sebagai obat malaria. Tanaman-tanaman yang memiliki efek antimalaria antara lain, Sambiloto (Andrographis paniculata Nees), Mundu (Garcinia dulcis Kurz), Anting-anting (Acalypha indica L.), Johar (C. siamea Lamk), Pasak bumi (Eurycoma longifolia jack), Ketumpang (Tridax procumbens L), Cocor bebek (Kalanchoe blossfeldiana Poelln), dan Talikuning (Anamirta cocculus). Meskipun efek samping dari obat-obatan tradisional relatif kecil, tetapi keamanan obat-obatan tradisional dalam praktik klinis yang baik harus dipertimbangkan. Kata kunci : Tanaman Obat ,Malaria, Plasmodium, Antimalaria, Keamanan Abstract Malaria is one of the infectious diseases with high prevalence. This disease has become extremely serious because of the resistance of its parasites. The abundance of biodiversity in Indonesia spurs a lot of researches for new medicinal ingredients of various treatments, especially malaria. The use of medicinal plants as antimalaria agents has been widely carried out and developed. Based on the results of research about antimalaria agent in the form of whole plants, simplicia, extract and isolated compounds, many active compounds were found in the plants. This article is a literature study to review traditional medicines from various references. Some medicinal plants have been studied to demonstrate pharmacological effects as antimalaria. These plants are for example; Sambiloto (Andrographidis paniculata Nees), Mundu (Garcinia dulcis Kurz), Anting-anting (Acalypha indica L.), Johar (C. siamea), Pasak bumi (Eurycoma longifolia jack), Ketumpang (Tridax procumbens L), Cocor bebek (Kalanchoe blossfeldiana Poelln), and Talikuning (Anamirta cocculus). Although the side effects of the traditional medicines are relatively small, but safety of traditional medicines for their good clinical practice should be considered. Key words: Medicinal plants, Malaria, Plasmodium, Antimalaria, safety
Antibacterial activity extract fraction of black garlic against bacteria propionibacterium acnes with disc diffusion method Pandi, Rensiana Kartini; Jawa La, Elisabeth Oriana; Esati, Ni Ketut
Health Sciences and Pharmacy Journal Vol. 6 No. 3 (2022)
Publisher : STIKes Surya Global Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32504/hspj.v6i3.732

Abstract

Black garlic is the result of processing garlic through a heating process at a temperature of 70oC with a relative humidity of 70-80% for 21 days without additional treatment so that the water content decreases. Black garlic has antibacterial activity due to the presence of bioactive compounds from the phenolic and flavonoid groups. This study aims to determine the secondary metabolite content and antibacterial activity of the black garlic extract fraction against Propionibacterium acnes bacteria. Extraction of Black garlic by maceration method used 96% ethanol as solvent and to obtain extract fraction of Black garlic by multilevel fractionation method using n-hexane, ethyl acetate, and ethanol as solvents. To determine the content of secondary metabolites, phytochemical screening was carried out in each fraction. Antibacterial activity test of Propionibacerium acnes used disc diffusion method with various concentrations of n-hexane, ethyl acetate, ethanol fractions 2%, 4%, 6%, 8%, 10%, positive control (clindamycin 1%), negative control and media control. Data analysis was carried out with qualitative, quantitative, and statistical analysis. The results obtained were the extract fraction of black garlic contains secondary metabolites of flavonoids, alkaloids, saponins, and tannins. The results of the antibacterial activity test showed that the garlic extract fraction had antibacterial activity seen from the presence of an inhibition zone. The largest inhibition zone in the n-hexane fraction of 10% concentration of 8.50 ± 0.10 mm was included in the medium category. The positive control was 12.02 ± 1.41 mm and the negative control gave no inhibition zone.