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Sarlinda, Febrina
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Chemical Engineering, Chemistry & Bioengineering Engineering Environmental Science Health Professions Public Health

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Efektivitas Ecoenzyme dari Kulit Buah sebagai Disinfektan Lantai yang Ramah Lingkungan Sarlinda, Febrina; Khoiriyah, Yustin Nur
Jurnal Kesehatan Vol 14 No 3 (2023): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v14i3.4254

Abstract

Lantai merupakan salah satu area yang rentan menjadi tempat berkumpulnya kuman dan bakteri. Bakteri patogen yang sering ditemui di lantai antara lain E.coli, Pseudomonas aeruginosa, Enterobacter cloacae, Salmonella sp, Staphylococcus aureus yang semuanya dapat menyebabkan terjadinya berbagai macam penyakit. Penelitian ini bertujuan untuk mengetahui efektifitas ecoenzyme dari sampah kulit buah sebagai pembersih lantai desinfektan. Penelitian eksperimen ini dirancang dengan variabel jenis ecoenzyme (dari kulit buah asam dan kulit buah manis) serta variabel konsentrasi ecoenzyme (5%, 10%, 15%, 20, 40%, 60%, 80%) dengan pembanding desinfektan pembersih lantai komersil yang mengandung bahan aktif pine oil. Hasil analisis menunjukkan bahwa ecoenzyme efektif menurunkan jumlah angka kuman pada lantai. Hasil uji two way anova menunjukkan adanya perbedaan signifikan antara kedua jenis ecoenzyme dan konsentrasi ecoenzyme dalam menurunkan angka kuman lantai. Ecoenzyme dari kulit buah manis lebih efektif menurunkan jumlah angka kuman lantai daripada ecoenzyme kulih buah asam. Semakin tinggi konsentrasi econzyme yang digunakan sebagai cairan disinfektan, semakin tinggi persentase pengurangan jumlah angka kuman lantai. Pada konsentrasi ecoenzyme 80%, ecoenzyme kulit buah manis mampu menurunkan angka kuman hingga 86,16% dan ecoenzyme kulit buah asam 78,29%. Dibandingkan dengan disinfektan dari pembersih lantai komersial konsentrasi 0,5%, persentase penurunan angka kuman lantai setara dengan ecoenzyme kulit buah asam pada konsentrasi 71,8% dan ecoenzyme kulit buah manis pada konsentrasi 32%.
Potensi Pemanfaatan Enzim Biokatalitik dari Kulit Buah dan Sayur untuk Pengolahan Limbah Cair Rumah Sakit Sarlinda, Febrina; Fikri, Ahmad; Usman, Sarip; Ginting, Daria
Jurnal Pengendalian Pencemaran Lingkungan (JPPL) Vol. 6 No. 1 (2024): JPPL, Maret 2024
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat (P3M)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35970/jppl.v6i1.2263

Abstract

Health services in hospitals produce hazardous and infectious waste that comes from sharing sources of activity. The research carried out experimentally in laboratory aims to determine the effect of biocatalytic enzyme treatment from fruit and vegetable peel (concentration variations of 5%, 10%, 15%) on pH, chemical oxygen demand (COD) levels, total suspended solids (TSS), and total coliforms of hospital liquid waste. From the research results, it was found that the addition of biocatalytic enzymes was very effective in reducing the total coliform concentration. The highest reduction in coliforms occurred with the addition of 15% enzyme, where there was a decrease from 1600 MPN/100 mL to 500 MPN/100 mL at a digestion time of less than 24 hours. After a digestion time of 2 days, no coliforms were found in the wastewater samples. However, there was a decrease in pH and an increase in TSS and COD concentrations which did not meet quality standards after being treated with enzymes at all concentration variations. The profile of changes in TSS and COD concentrations shows a decreasing trend with an increase in digestion time of 1 to 3 days, the values ​​obtained will still exceed the TSS and COD quality standards (30 mg/L and 100 mg/L). Meanwhile, the waste pH gradually increased with increasing digestion time up to 3 days at enzyme concentrations of 5% and 10%, namely 3.5 and 4.8. However, this value is still outside the permitted quality standard interval, namely 6-9.
INSENSITIFITAS ASETILKOLINESTERASE (AChE) NYAMUK Aedes aegypti TERHADAP MINYAK SERAI WANGI (Cymbopogon nardus) Khoiriyah, Yustin Nur; Sarlinda, Febrina
Jurnal Kesehatan Vol 15 No 3 (2024): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v15i3.4751

Abstract

Aedes mosquitoes are vectors of viruses, namely the dengue virus. Treatment with insecticides is no longer effective if the target mosquito is resistant. Therefore, efforts are needed to overcome the resistance of these mosquitoes. Inhibition of the enzyme acetylcholinesterase (AChE) is one way the synthetic insecticide Profenofos works; if AChE is inhibited, acetylcholine accumulation will disrupt the nervous system. Insensitivity to AChE is one mechanism of mosquito resistance to insecticides. Based on this, the problem is formulated as follows: is the increase in susceptibility of Aedes aegypti mosquitoes to alphacymethrin, citronella oil, and a combination of both caused by a decrease in the insensitivity of mosquito AChE? This study aims to determine the AChE insensitivity of Aedes aegypti mosquitoes to citronella oil, a combination of citronella oil and alphacymethrin 0.03%), compared to the positive control (insecticide alphacymethrin 0.03%). Percentage of mosquito mortality after being held for 24 hours: The results showed no difference between the two groups of mosquitoes without treatment (A) and 100 µL/L citronella oil (B), F count 0.000, Sig. 1.000, significance value <0.05. Exposure to 100 µL/L citronella oil on instar III-IV mosquito larvae did not make mosquitoes more susceptible or more resistant to 0.03% alphacypermethrin insecticide. Based on the absorption value analysis results using the T-test between group A/ without treatment and group B with 100µL/L citronella oil treatment showed a Sig. (2-tailed) value of 0.547>0.05 means there is no significant difference between the two groups.
Potensi Pemanfaatan Enzim Biokatalitik dari Kulit Buah dan Sayur untuk Pengolahan Limbah Cair Rumah Sakit Sarlinda, Febrina; Fikri, Ahmad; Usman, Sarip; Ginting, Daria
Jurnal Pengendalian Pencemaran Lingkungan (JPPL) Vol. 6 No. 1 (2024): JPPL, Maret 2024
Publisher : Pusat Penelitian dan Pengabdian Kepada Masyarakat (P3M), Politeknik Negeri Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35970/jppl.v6i1.2263

Abstract

Health services in hospitals produce hazardous and infectious waste that comes from sharing sources of activity. The research carried out experimentally in laboratory aims to determine the effect of biocatalytic enzyme treatment from fruit and vegetable peel (concentration variations of 5%, 10%, 15%) on pH, chemical oxygen demand (COD) levels, total suspended solids (TSS), and total coliforms of hospital liquid waste. From the research results, it was found that the addition of biocatalytic enzymes was very effective in reducing the total coliform concentration. The highest reduction in coliforms occurred with the addition of 15% enzyme, where there was a decrease from 1600 MPN/100 mL to 500 MPN/100 mL at a digestion time of less than 24 hours. After a digestion time of 2 days, no coliforms were found in the wastewater samples. However, there was a decrease in pH and an increase in TSS and COD concentrations which did not meet quality standards after being treated with enzymes at all concentration variations. The profile of changes in TSS and COD concentrations shows a decreasing trend with an increase in digestion time of 1 to 3 days, the values ​​obtained will still exceed the TSS and COD quality standards (30 mg/L and 100 mg/L). Meanwhile, the waste pH gradually increased with increasing digestion time up to 3 days at enzyme concentrations of 5% and 10%, namely 3.5 and 4.8. However, this value is still outside the permitted quality standard interval, namely 6-9.
Co-Authors Ahmad Fikri Ginting, Daria Usman, Sarip Yustin Nur Khoiriyah