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All Journal JURNAL ILMIAH PLATAX
Pangkey, Henneke D.
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Antibacterial Activity and Zoochemical Analysis of Sea Urchin Diadema setosum (Leske, 1778) Extract From Aertembaga Waters, Bitung City Rompas, Gebriela; Lintang, Rosita A. J.; Sumilat, Deiske A. A.; Rumengan, Inneke F. M.; Ginting, Elvy L.; Pangkey, Henneke D.
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42322

Abstract

Sea urchin is one of the marine biotas that produce bioactive compounds and has biological activity, one of which is antibacterial. This study aims to determine the antibacterial activity of the crude extract of D. setosum gonad and its fractionation against test bacteria Staphylococcus aureus and Escherichia coli and to conduct a zoochemical analysis to determine the content of bioactive compounds. The antibacterial test used the disc diffusion method while the chemical analysis was carried out qualitatively. The results showed that the methanol, ethyl acetate, and n-hexane fraction had antibacterial activity against the two test bacteria. The ethyl acetate fraction was the fraction that showed the highest antibacterial activity, its inhibition zone was 8 mm against S. aureus and 7,5 mm against E. coli. The zoochemical analyses of the ethyl acetate fraction from gonads extract showed positive results for alkaloid compound, phenolic, and saponinKeywords: Sea urchin Gonad, Antibacterial, Disc diffusion, Zoochemistry, ExtractionAbstrakBulu babi merupakan salah satu biota laut yang memproduksi senyawa bioaktif dan memiliki aktivitas biologis salah satunya adalah antibakteri. Tujuan penelitian ini mengetahui aktivitas antibakteri dari ekstrak kasar gonad bulu babi D. setosum dan hasil fraksinasi terhadap bakteri Staphylococcus aureus dan Escherichia coli serta melakukan analisis zookimia untuk mengetahui kandungan senyawa bioaktif. Pengujian antibakteri menggunakan metode difusi cakram sedangkan analisis kimia dilakukan secara kualitatif. Hasil penelitian menunjukkan baik fraksi metanol, fraksi etil asetat, dan fraksi n-heksana memiliki aktivitas antibakteri terhadap kedua bakteri uji. Fraksi etil asetat merupakan fraksi yang memperoleh aktivitas antibakteri tebaik dengan diameter zona hambat 8 mm terhadap bakteri S.aureus dan 7,5 mm terhadap bakteri E. coli. Hasil uji zookimia fraksi etil asetat menunjukkan bahwa ekstrak gonad D. setosum mengandung senyawa dari golongan alkaloid, fenolik, dan saponin.Kata kunci : Gonad Bulu Babi (D. setosum ), Antibakteri, difusi cakram, Zookimia, Ekstraksi
Phytoplankton Community Structure in Seagrass Beds in Tiwoho Village, North Minahasa Regency Rimper, Abraham M.; Warouw, Veibe; Rimper, Joice R.T.S.L; Lintang, Rosita A.J.; Ompi, Medy; Pangkey, Henneke D.
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.45232

Abstract

This research was carried out from May to July 2022. A sampling at the study site included seawater for the needs of plankton identification as well as the measurement of water nitrate & phosphate levels and measurement of physical and chemical parameters such as temperature, pH, salinity and dissolved oxygen which were carried out on an ongoing basis In situ (in the field). The technique for determining plankton sampling was carried out purposively, namely with certain considerations to obtain samples that represent the research location area. Plankton sampling was carried out horizontally using a plankton net. Sample bottles containing seawater were identified at the Marine Biology Laboratory, Faculty of Fisheries and Marine Sciences. The composition of the phytoplankton found in the Tiwoho seagrass bed ecosystem consists of three classes, namely the Bacillariophyceae, Dynophyceae, and Cyanophyceae classes, with 28 genera. The results of chopped phytoplankton are expressed in Cells/l. The qualitative determination of plankton is made up to the genus level. The composition of the phytoplankton found in the Tiwoho seagrass bed ecosystem consists of three classes, namely the Bacillariophyceae, Dynophyceae, and Cyanophyceae classes, with 28 genera. The results of calculating the abundance of phytoplankton in the Tiwoho seagrass ecosystem are in the range of 29 - 66 cells/l. The highest abundance was found at station two, namely 66 cells/l with 21 genera, then station three (65 cells/l) with 24 genera, and station one (29 cells/l) with a total of 23 genera. The diversity index of seagrass beds in Tiwoho waters is in the range of 1.9330 - 2.4083, which means that community stability is categorized as moderate. The uniformity index is in the range of 0.4614 - 0.7154, this means that the uniformity between species is relatively the same, or the difference is not striking. While the domination index is in the range of 0.1494 - 0.2404, it means that the condition of the community structure is stable, and there is no ecological pressure (stress) on biota. Keywords: Phytoplankton, Biological Index, Abundance, Environmental parameters Abstrak Penelitian ini dilaksanakan pada bulan Mei sampai bulan Juli 2022. Pengambilan sampel di lokasi penelitian meliputi air laut untuk kebutuhan identifikasi fitoplankton dan pengukuran kadar nitrat, fosfat perairan serta pengukuran parameter fisika kimia seperti suhu, pH, salinitas dan oksigen terlarut yang dilakukan secara In situ (di lapangan). Teknik penentuan pengambilan sampel plankton dilakukan secara purposif yaitu dengan pertimbangan tertentu untuk mendapatkan sampel yang mewakili area lokasi penelitian. Pengambilan sampel plankton dilakukan secara horisontal dengan menggunakan plankton net. Sampel plankton diberi pengawet formalin 4% dan diidentifikasi di laboratorium Biologi Kelautan Fakultas Perikanan dan Ilmu Kelautan. Komposisi fitoplankton yang dijumpai di ekosistem padang lamun Perairan Tiwoho terdiri dari tiga kelas yaitu kelas Bacillariophyceae, Dynophyceae, dan Cyanophyceae, dengan 28 genera. Hasil perhitungan kelimpahan fitoplankton di ekosistem padang lamun Perairan Tiwoho berada di kisaran 29 - 66 sel/l. Kelimpahan tertinggi ditemukan di stasiun dua (depan kampung) yaitu 66 sel/l dengan 21 genera, kemudian stasiun tiga (depan dermaga) yaitu 65 sel/l dengan 24 genera, dan stasiun satu (ujung kampung) sebanyak 29 sel/l dengan jumlah 23 genera. Indeks keanekaragaman ekosistem padang lamun Perairan Tiwoho berada pada kisaran 1,9330 - 2,4083, yang berarti stabilitas komunitas dikategorikan sedang. Indeks keseragaman yaitu pada kisaran 0,4614 - 0,7154, ini berarti keseragaman antar spesies relatif sama, atau perbedaannya tidak menyolok. Sedangkan indeks dominasi yaitu pada kisaran 0,1494 - 0,2404, berarti kondisi struktur komunitas dalam keadaan stabil, dan tidak terjadi tekanan ekologi (stress) terhadap biota. Kata kunci: Fitoplankton, Indeks Biologi, Kelimpahan, Parameter lingkungan