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All Journal JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI
Elvy L. Ginting, Elvy L.
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Earth & Planetary Sciences Education Environmental Science

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ISOLASI BAKTERI SIMBION DENGAN SPONS DARI PERAIRAN TONGKEINA, SULAWESI UTARA Wantania, Letha L.; Ginting, Elvy L.; Wullur, Stenly
JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI Vol 3, No 1 (2016)
Publisher : JURNAL LPPM BIDANG SAINS DAN TEKNOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini dilakukan dengan tujuan untuk mengisolasi bakteri yang bersimbiosis denganspons dan menentukan karakteristik morfologi serta sifat Gram dari isolat bakteri tersebut. Sampel spons diambil dari perairan Tongkeina, Sulawesi Utara. Spons digerus dan dilarutkan dalam air laut steril dan dilakukan pengenceran. Bakteri simbions spons dalam setiap pengenceran ditumbuhkan pada media Nutrient Broth. Bakteri yang tumbuh ditumbuhkan kembali pada media Nutrient Agar dengan metode cawan gores. Isolat bakteri yang tumbuh kemudian diuji sifat Gramnya. Penelitian ini memperoleh sepuluh isolat bakteri yang bersimbiosis dengan dua jenis spons dengan karaktersitik morfologi yang bervariasi. Dua dari kesepuluh isolat bakteri tergolong dalam Gram positif dengan bentuk coccus dan delapan tergolong dalam Gram negatif dengan bentuk basil (batang pendek).
Identifikasi Mikroba yang Koeksis Dengan Ascidia Lissoclinum patella Menggunakan Sekuens Gen 16S rRNA Untu, Patricia; Rumengan, Inneke F. M.; Ginting, Elvy L.
JURNAL PESISIR DAN LAUT TROPIS Vol 3, No 2 (2015): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.3.2.2015.10110

Abstract

Penelitian ini dilakukan dengan tujuan untuk menentukan jenis mikroba koeksis denganascidia Lissoclinum patella menggunakan sekuens gen 16S rRNA. Sampel yang digunakandalam penelitian ini diambil dari jaringan tissue pada ascidia L. patella yang diambil dariperairan Malalayang, Sulawesi Utara. Sampel mikroba diinokulasi dalam media Hirata dandikultur selama ± 1 minggu. Sampel mikroba tersebut diisolasi DNA, amplifikasi melalui PCR(Polymerase Chain Reaction), elektroforesis gel agarose dan dianalisis data DNAnyamenggunakan BLAST pada NCBI (National Center for Biotechnology Information). Identifikasiyang dilakukan menggunakan BLAST diperoleh hasil 15 mikroba yang memiliki tingkatkemiripan yang tinggi dengan sekuens gen 16S rRNA sampel mikroba yaitu cyanobacteriumenrichment culture CAWBG121 dan CAWBG120 clone, uncultured Symploca sp. clone DRTO-55, Leptolyngbya sp. PCC7376 complete genome, Leptolyngbya sp. PCC7376, unculturedbacterium clone PINFEBB02, uncultured bacterium clone 5M47, Synechococcus elongatusCCMP1630, uncultured bacterium clone reef H09, Synechococcus sp. PCC7002 completegenome, Synechococcus sp. 16S rRNA gene strain PCC7002, Synechococcus sp. DNA untuk16 ribosomal RNA, Synechoccous sp. L21-BG-1, Oscillatoria rosea IAM M-220 danSynechococcus sp. PCC 8807. Tingkat kemiripan mikroba dalam NCBI dengan sampel H1berkisar antara 98-99 %.
Antibacterial Activity and Zoochemical Analysis of Sea Urchin Diadema setosum (Leske, 1778) Extract From Aertembaga Waters, Bitung City Rompas, Gebriela; Lintang, Rosita A. J.; Sumilat, Deiske A. A.; Rumengan, Inneke F. M.; Ginting, Elvy L.; Pangkey, Henneke D.
Jurnal Ilmiah Platax Vol. 10 No. 2 (2022): ISSUE JULY-DECEMBER 2022
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v10i2.42322

Abstract

Sea urchin is one of the marine biotas that produce bioactive compounds and has biological activity, one of which is antibacterial. This study aims to determine the antibacterial activity of the crude extract of D. setosum gonad and its fractionation against test bacteria Staphylococcus aureus and Escherichia coli and to conduct a zoochemical analysis to determine the content of bioactive compounds. The antibacterial test used the disc diffusion method while the chemical analysis was carried out qualitatively. The results showed that the methanol, ethyl acetate, and n-hexane fraction had antibacterial activity against the two test bacteria. The ethyl acetate fraction was the fraction that showed the highest antibacterial activity, its inhibition zone was 8 mm against S. aureus and 7,5 mm against E. coli. The zoochemical analyses of the ethyl acetate fraction from gonads extract showed positive results for alkaloid compound, phenolic, and saponinKeywords: Sea urchin Gonad, Antibacterial, Disc diffusion, Zoochemistry, ExtractionAbstrakBulu babi merupakan salah satu biota laut yang memproduksi senyawa bioaktif dan memiliki aktivitas biologis salah satunya adalah antibakteri. Tujuan penelitian ini mengetahui aktivitas antibakteri dari ekstrak kasar gonad bulu babi D. setosum dan hasil fraksinasi terhadap bakteri Staphylococcus aureus dan Escherichia coli serta melakukan analisis zookimia untuk mengetahui kandungan senyawa bioaktif. Pengujian antibakteri menggunakan metode difusi cakram sedangkan analisis kimia dilakukan secara kualitatif. Hasil penelitian menunjukkan baik fraksi metanol, fraksi etil asetat, dan fraksi n-heksana memiliki aktivitas antibakteri terhadap kedua bakteri uji. Fraksi etil asetat merupakan fraksi yang memperoleh aktivitas antibakteri tebaik dengan diameter zona hambat 8 mm terhadap bakteri S.aureus dan 7,5 mm terhadap bakteri E. coli. Hasil uji zookimia fraksi etil asetat menunjukkan bahwa ekstrak gonad D. setosum mengandung senyawa dari golongan alkaloid, fenolik, dan saponin.Kata kunci : Gonad Bulu Babi (D. setosum ), Antibakteri, difusi cakram, Zookimia, Ekstraksi
Condition of Seagrass Meadows in The Waters Around The Sunrise Tourist Area, Makalisung Village, Kombi District, Minahasa Regency Baso, Fathan; Wagey, Billy T.; Sondak, Calvyn F. A.; Ginting, Elvy L.; Tilaar, Sandra O.; Rampengan, Royke M.
JURNAL PESISIR DAN LAUT TROPIS Vol. 14 No. 1 (2026): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.14.1.2026.65793

Abstract

Seagrass is a flowering plant (magnoliopyta). Seagrass requires a minimum light intensity of $11% - 25%$ for photosynthesis, and disturbances can reduce the availability of light. Research in the Sunrise Tourism Area, Makalisung Village, aims to identify the types and conditions of seagrass meadows, considering the pressure from human activities such as garbage disposal and fishing boat traffic. The data collection method uses quadrant transects with three 100-meter transects, each placed in 11 quadrants. The results of the study successfully identified 7 types of seagrass, including Cymodocea rotundata, Enhalus acoroides, Halophila ovalis, Halodule pinifolia, Halodule uninervis, Syringodium isoetifolium, and Thalassia hemprichii. Based on calculations, the average percentage of seagrass cover is 57.68% and can be categorized as dense. However, although the cover is classified as dense, Meanwhile, according to the Ministry of Environment Decree No. 200/2004, the determination of the closure of seagrass meadow conditions can be categorized as less rich/less healthy. This indicates the potential for environmental problems that can affect the health of the seagrass ecosystem at Sunrise Beach. Keywords: seagrass condition, Sunrise Beach, coastal waters, seagrass cover Abstrak Lamun (seagrass) merupakan tumbuhan berbunga (magnoliopyta). Lamun memerlukan intensitas cahaya minimal $11% - 25%$ untuk fotosintesis, dan gangguan tersebut dapat mengurangi ketersediaan cahaya. Penelitian di Kawasan Wisata Sunrise, Desa Makalisung, bertujuan mengidentifikasi jenis dan kondisi padang lamun, mengingat adanya tekanan dari aktivitas manusia seperti pembuangan sampah dan lalu lintas perahu nelayan. Metode pengambilan data menggunakan transek kuadran dengan tiga transek sepanjang  100 meter, masing- masing diletakkan 11 kuadran. Hasil penelitian berhasil mengidentifikasi 7 jenis lamun, termasuk Cymodocea rotundata, Enhalus acoroides, Halophila ovalis, Halodule pinifolia, Halodule uninervis, Syringodium isoetifolium, dan Thalassia hemprichii.Berdasarkan perhitungan, rata-rata persentase tutupan lamun adalah 57,68% dan dapat dikategorikan padat.Namun, meskipun tutupan tergolong padat, Sedangkan menurut KEPMEN KLH No 200/2004, penentuan penutupan kondisi padang lamun dapat dikategorikan dalam kategori kurang kaya/kurang sehat. Hal ini menunjukkan adanya potensi masalah lingkungan yang dapat memengaruhi kesehatan ekosistem lamun di Pantai Sunrise. Kata kunci: kondisi lamun, Pantai Sunrise, perairan pesisir, tutupan lamun
Tracing The CRISPR System in The Genomes of The Oscillatoria acuminata and Stanieria cyanosphaera Originated from Malalayang Waters Laliboso, Jurwin A.; Rumengan, Inneke; Ginting, Elvy L.; Manembu, Indri S.; Mantiri, Desy M. H.; Kawung, Nickson J.
JURNAL PESISIR DAN LAUT TROPIS Vol. 14 No. 1 (2026): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.14.1.2026.66220

Abstract

The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system is a specific nucleotide sequence in genomes of certain bacteria and archaea that functions as an adaptive immune system against viral infections and foreign genetic elements. However, not all microorganisms possess CRISPR systems; therefore, bioinformatic approaches are required to trace the occurrence of CRISPR-Cas systems. The objective of this study was to detect CRISPR-Cas systems in genomes of Oscillatoria acuminata and Stanieria cyanosphaera. Both species were identified among 2679 operational taxonomic units (OTUs) of microorganisms associated with ascidian Lissoclinum patella originated from Malalayang waters, based on previously reported metagenomic analyses. These species were selected due to their relatively high abundance and the availability of complete genome sequence data in the NCBI database, which were downloaded as FASTA format and analyzed using CRISPRCasFinder software. The results showed that O. acuminata possesses a complete genome sequence of 7,689,443 bp, containing 80 CRISPR arrays, of which two arrays are associated with Cas systems, and a total of 338 spacers. In the other hand, S. cyanosphaera has a complete genome sequence of 5,041,209 bp, with eight CRISPR arrays, two of which are associated with Cas systems, and a total of 37 spacers. The detected CRISPR loci were visualized using CRISPRCasViewer in three display models: linear, circular, and scatter plot. Further studies are required to determine the specific types of CRISPR-Cas systems present in each microbial genome. Keywords: O. acuminata; S. cyanosphaera; bioinformatics; CRISPRCasFinder; CRISPRCasViewer   Abstrak Sistem CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) merupakan susunan sekuens nukleotida spesifik dalam genom bakteri dan arkaea tertentu yang berfungsi sebagai  sistem imun adaptif mikroba terhadap serangan virus dan materi genetik asing. Namun, tidak semua mikroba memiliki sistem CRISPR, sehingga diperlukan pendekatan analisis bioinformatika untuk mendeteksi CRISPR. Tujuan penelitian ini adalah untuk menelusuri keberadaan sistem CRISPR-Cas pada genom Oscillatoria acuminata dan Stanieria cyanosphaera. Kedua 2 jenis mikroba ini ditemukan di antara 2679 operational taxonomic units (OTUs) mikroba yang berasosiasi dengan ascidia Lissoclinum patella dari perairan Malalayang, berdasarkan analisis metagenom yang telah dilaporkan sebelumnya.  Kedua spesies ini dipilih karena terdeteksi cukup melimpah, dan ketersediaan data urutan genom lengkap di NCBI. Data urutan genom diunduh dalam format FASTA dan dianalisis dengan perangkat lunak CRISPRCasFinder. Ternyata O. acuminata memiliki  sekuens genom  lengkap  7.689.443 bp dengan 80 unit  sistem CRISPR, dimana ada 2 unit dengan sistem Cas, serta 338 spacer. Sedangkan S. cyanosphaera memiliki sekuens genom lengkap sekitar 5.041.209 bp dengan 8 unit CRISPR, dan 2 unit dengan sistem Cas serta 37 spacer. Hasil deteksi CRISPR divisualisasikan menggunakan CRISPRCasViewer dalam tiga model tampilan, yaitu Linear, Circular, dan Scatter Plot. Selanjutnya, ke depan perlu penelusuran lebih lanjut untuk menentukan tipe CRISPR-Cas pada setiap genom mikroba tersebut. Kata Kunci: O. acuminata; S. cyanosphaera; bioinformatika; CRISPRCasFinder; CRISPRCasViewer  
Determination of Dominant Mangrove Species and Comparison of Tree and Sapling Composition in The Mangrove Area of Sonsilo Village, West Likupang District, North Minahasa Regency Mandei, Kania F.A.; Rumengan, Antonius P.; Paruntu, Carolus P.; Manembu, Indri S.; Ginting, Elvy L.; Djamaluddin, Rignolda
JURNAL PESISIR DAN LAUT TROPIS Vol. 14 No. 1 (2026): JURNAL PESISIR DAN LAUT TROPIS
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jplt.14.1.2026.66590

Abstract

Mangrove forests are key ecosystems in tropical coastal areas that function as environmental stability guards through physical roles (preventing erosion and tsunamis), chemical (absorbing pollutants and storing carbon), and biological (marine biota habitat and source of biodiversity). This study aims to identify the species and determine the dominant species of mangroves and the comparison of tree and sapling composition in the mangrove area of ​​Sonsilo Village, West Likupang District, North Minahasa Regency.  The research was conducted over three months (September–November 2024) using a purposive sampling method to collect data on density, canopy cover, and species frequency. The results revealed seven mangrove species: A. marina, B. gymnorrhiza, C. tagal, R. apiculata, R. mucronata, S. alba, and X. granatum.  Among these, three species (B. gymnorrhiza, R. apiculata, and R. mucronata) were dominant at both tree and sapling levels, exhibiting the highest Importance Value Index (IVI).  Mangrove species diversity in the area is categorized as low to moderate, as indicated by the Shannon-Wiener Diversity Index (H'), reflecting that species richness is not yet fully optimal. Analysis of the Dominance Index (D) and Evenness Index (J') suggests a relatively even distribution of individuals among species.  The study recommends regular monitoring and evaluation, as well as conservation efforts, to maintain the stability of the mangrove ecosystem and support its sustainable use. These findings highlight the importance of biodiversity management in preserving the ecological balance of mangrove habitats. Keywords: Sonsilo Village, mangroves, mangroves composition
Co-Authors Antonius P. Rumengan Baso, Fathan Billy Theodorus Wagey Calvyn F. A. Sondak, Calvyn F. A. Carolus Paulus Paruntu Indri Manembu Inneke F. M Rumengan Laliboso, Jurwin A. Lintang, Rosita AJ Mandei, Kania F.A. Mantiri, Desy M. H Nickson J. Kawung, Nickson J. Pangkey, Henneke D. Patricia Untu, Patricia Rignolda Djamaluddin Rompas, Gebriela Royke M. Rampengan Sandra Tilaar Stenly Wullur Sumilat, Deiske A. A. Wantania, Letha L.