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Runtunuwu, Semuel
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology Social Sciences

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Barcode DNA berdasarkan Gen rbcL dan matK Anggrek Payus Limondok (Phaius tancarvilleae) (DNA Barcode of Payus Limondok Orchid (Phaius tancarvilleae) Based on the rbcL and matK genes) Kolondam, Beivy J; Lengkong, Edy; J. Polii, Mandang; Pinaria, Arthur; Runtunuwu, Semuel
JURNAL BIOS LOGOS Vol 2, No 2 (2012): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.2.2.2012.1041

Abstract

Metode identifikasi spesies telah disepakati menggunakan barcode DNA standar yaitu gen rbcL dan gen matK. Tujuan penelitian ini untuk menentukan tingkat kemiripan sekuens barcode DNA tanaman Anggrek Payus Limondok (Phaius tancarvilleae) dengan spesies kerabatnya yang sudah terdata dalam BOLD Systems, merekomendasi penggunaan barcode untuk mengidentifikasi atau mengkonfirmasi spesies ini, dan mengamati variasi intraspesifik. Teknik Polymerase Chain Reaction (PCR) digunakan untuk mengamplifikasi sekuens gen rbcL dan matK melalui primer universal. Barcode rbcL menunjukkan kemiripan 100% (identik) dengan dua spesies berbeda dalam famili yang sama (Orchidaceae), sehingga tidak bisa diandalkan untuk identifikasi spesies P. tancarvilleae secara akurat. Sekuens matK sampel menghasilkan kemiripan 100% dengan spesies sama yang sebelumnya telah terdata dalam BOLD Systems. Kemiripan ini mengindikasikan rendahnya variasi genetik intraspesies tetapi sekuens matK dapat diandalkan untuk identifikasi atau konfirmasi spesies anggrek P. tancarvilleae.Kata Kunci: barcode, rbcL, matK, Phaius tancarvilleae AbstractSpecies identification methods convention have been recommended to use standard DNA barcode for plants; the rbcL and matK genes. The aims of this research were to determine similarities in barcode DNA sequences of Payus Limondok Orchid (Phaius tancarvilleae) with its close relatives that listed in BOLD Systems, to recommend the use of DNA barcodes for identification or confirmation of this species, and to observe intraspecific variations. Polymerase Chain Reaction technique was employed to amplify rbcL and matK genes using universal primers. The rbcL barcode of Payus Limondok resulted identical hit with other two different species in the same family (Orchidaceae), therefore, unreliable for accurate P. tancarvilleae species identification. The matK sequence of this plant was 100% similar with the same plant species listed in BOLD Systems. This similarity indicated low genetic variation within the species, but the matK sequence was found to be reliable for P. tancarvilleae orchid species identification or confirmation.Keywords: barcode, rbcL, matK, Phaius tancarvilleae
Substitusi Media Murashige dan Skoog/MS dengan Air Kelapa dan Pupuk Daun Majemuk pada Pertumbuhan Anggrek Dendrobium secara in vitro (In Vitro Growth of Dendrobium Orchids under Substitution Murashige dan Skoog/MS Medium With Coconut Water and Compound Leaf Fertilizer) Inkiriwang, Annatje E.B.; Mandang, Jeany; Runtunuwu, Semuel
JURNAL BIOS LOGOS Vol 6, No 1 (2016): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.6.1.2016.16258

Abstract

Abstrak  Penelitian ini bertujuan  untuk  menentukan  konsentrasi  substitusi air kelapa dan pupuk   daun  majemuk  yang tepat    pada   media  MS   terhadap   pertumbuhan   anggrek Dendrobium secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 7 perlakuan dan 10 kali ulangan.  Variabel yang diamati adalah persentase eksplan yang bertunas, jumlah tunas, jumlah daun, tinggi tanaman dan jumlah akar. Hasil penelitian menunjukkan substitusi media MS 50 %, air kelapa 30 % dan pupuk daun majemuk (Growmore®) 1,5 g/L menghasilkan rata-rata persentase eksplan yang bertunas sebesar 6,78 %; jumlah tunas sebesar 1,26 dan tinggi tanaman sebesar 1,20 cm.Kata kunci: air kelapa, in vitro, media MS, pupuk daun majemuk  Abstract             This research was aimed to determine the proper concentration of substitution of coconut water and compound leaf fertilizer in Murashige dan Skoog (MS) medium on the in vitro growth of Dendrobium orchids. The completely randomized design experiment consisted of 7 treatments and 10 replicates. The measured parameters were the percentage of sprouted explants, number of shoots, number of leaves, plant height and number of roots. The result showed that the substitution of 50% media MS, 30% coconut water and 1.5 g/L leaf fertilizer (Growmore®) resulted the growth of shoot explant as much as 6.78% the shoot number of 1.26 and 1.20 cm plant height .Keywords: coconut water, compound leaf fertilizer, in vitro, MS medium 
Co-Authors Arthur G. Pinaria BEIVY JONATHAN KOLONDAM Edy Lengkong Horonis, Seistelin Inkiriwang, Annatje E.B. J. Polii, Mandang Jeany Mandang Johannes E. X. Rogi Legoh, Widya Lusye Rogi, Dianita Wanget, Sesilia A. Yefta Pamandungan