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All Journal Biology, Medicine, & Natural Product Chemistry The Indonesian Journal of Public Health Journal of Vocational Education Studies
Ismail, Zalina
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Biochemistry, Genetics & Molecular Biology Education Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Novel Approaches for Detection Fluorescent-Labeled by Cellvizio Lab System on Hippocampal CA1 Region Shaimi, Asma Ulhusna; Aasim, Wan Raihana Wan; Abdullah, Hasmah; Choon, Tan Soo; Wei, Ang Chee; Ismail, Zalina
Biology, Medicine, & Natural Product Chemistry Vol 8, No 1 (2019)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2019.81.1-6

Abstract

Neurosteroids have been identified in the 1981. Dehydroepiandrosterone sulphate (DHEAS) is one of the vital neurosteroids that de novo synthesized in the nervous system from cholesterol precursor (Baulieu & Robel, 1998). The aim of the study is to develop a method for fluorescence labelling. Alexa Fluor 488 dye with DHEAS antibody can binds the DHEAS antibody in the rat brain monitored by Cellvizio Lab System. DHEAS antibody (IgG isotype antibodies) was fluorescently conjugated by an amine-reactive compound, Alexa Fluor 5-SDP ester 488 dye. The resultant Alexa Fluor 488-conjugated antibodies were collected and analyzed by UV-Vis spectrophotometer instrument. The absorbance of the protein-dye conjugate at 280 nm and 494 nm were measured. Then, the degree of labeling (DOL) was calculated to achieve the desired results. Fluorescence labelling were carried out into the CA1 region of hippocampus Sprague-Dawley rat. We reported that the conjugation was successful. Optimal labeling depending on degree of labeling (DOL) needs some necessity to achieve and effective binding to the target neurosteroid, DHEAS. Cellvizio Lab system connected with Fiber Fluorescence Microscopy (FFM) probe is presented as a new approach in real-time imaging of DHEAS. In conclusion, we have developed a new method of DHEAS-Alexa Fluor fluorescence labelling to visualize and evaluate the changes of DHEAS fluorescence level in the rat hippocampus. This novel approach as a diagnostic tool and can be used to better understand the mechanisms and functions of DHEAS and other neurosteroids in future research.
Cultural Value Diversity: Influence towards Multicultural Teamwork Performance among Restaurant Employees Ab-Latif, Zahidah; Abdul Mutalib, Asilah; Ismail, Zalina; Ismail, Fazhana; Che Man, Shaibatul ' Islamiah
Journal of Vocational Education Studies Vol 3, No 2 (2020)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/joves.v3i2.2933

Abstract

Malaysia currently has a diverse workforce, relying on foreign workers in the hospitality industry. This study tested a conceptual model to investigate the role of specific cultural diversity value in influencing multicultural teamwork performance. A self-administered questionnaire was conducted in casual ethnic restaurants in Malaysia. The findings revealed that the three diversity dimensions of collectivism, determinism, and orientation each had distinctive effects on productivity and/or cooperation. This study offers new insights on cultural value diversity and multicultural teamwork performance at a dimensional level thus provides rigid answer for the argument from previous scholars. In addition, by providing the current phenomena in workforce industry, this study helps the restaurant managers to prepare with strategic management on handling diversity among multicultural team.
Induction Of Ovulation Using Ovaprim And Its Impact On Reproductive Parameter Of Clarias gariepinus Anak Gaung, Paul Morisson; Ismail, Zalina; Abdul Mutalib, Asilah; Ismail, Fazhana; Che Man, Shaibatul ’ Islamiah; Ab Latif, Zahidah
Journal of Vocational Education Studies Vol 3, No 2 (2020)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/joves.v3i2.2931

Abstract

Sustainability of the aquaculture industry can be secure through the induce breeding technique. One way that induce breeding can do is to increase the rate of maturation and ovulation in making sure the availability of good quality fish seed all year round. Here, we investigate how Ovaprim hormone impact the reproduction of Clarias gariepinusincluding spawning rate, fecundity, hatching and survival rate. For this purpose, Six broodstocks were divided in three experimental treatments and injected intramuscularly with different doses of Ovaprim hormone of T1: 0.25ml/kg for female and 0.125ml/kg for male, T2: 0.5ml/kg for female and 0.25ml/kg for male and T3: 1.0ml/kg for female and 0.5ml/kg for male. According to our results, broodstock injected with Ovaprim hormone of dose of T3: 1.0ml/kg for female and 0.5ml/kg for male showed higher eggs production and hatching rate compared to other treatment. The results of this study suggest that a dosage of 1.0ml/kg Ovaprim for female and 0.5ml/kg for male have a better effect on the reproductive parameter of Clarias gariepinus.
Effects Of Fish Amino Acid (Faa) Application on Growth and Development of Okra (Abelmoschus Esculentus) at Different Sampling Times Johari, Nor Syakina; Abdul Mutalib, Asila; Ismail, Zalina; Ismail, Fazhana; Ab Latif, Zahidah; Che Man, Shaibatul ' Islamiah; Tang, Jing Rui
Journal of Vocational Education Studies Vol 3, No 2 (2020)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/joves.v3i2.2932

Abstract

An experiment was conducted to determine the effects of fish amino acid (FAA) application on growth and development of okra (Abelmoschus esculentus) with different sampling times. It was placed at Soil Science Laboratory, Blok Pakar at the Universiti Pendidikan Sultan Idris (UPSI). The okra plant was treated with a different amount of FAA (5 mL and 10 mL). Total of experimental units were 24. The used experimental design was Randomised Complete Block Design (RCBD). The parameters such as root length, shoot length, plant biomass, soil pH and soil moisture content were analysed after 2 weeks and 4 weeks of transplanting. The data was analysed by using Analysis of Variance (ANOVA), Statistical Package for the Social Sciences (SPSS) and means comparison were using Tukey HSD test with significant level (P≤0.05). Results showed that FAA application and different sampling times were significantly affected the shoot length of okra. Besides, the volume of FAA and the number of weeks were significantly affected the soil pH and the soil moisture content, respectively. However, the addition of FAA application did not affect the root length and plant biomass of okra.
COMPARATIVE STUDY: HEALTH RISK BEHAVIOUR IN URBAN AND RURAL INDONESIAN ADOLESCENTS Vionalita, Gisely; Ismail, Zalina
The Indonesian Journal of Public Health Vol. 20 No. 1 (2025): THE INDONESIAN JOURNAL OF PUBLIC HEALTH
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijph.v20i1.2025.15-28

Abstract

Introduction: In 2019, the prevalence of cigarette smoking among adolescents in Indonesia increased to 18.8%. Aim: To assess health risk behaviours among Indonesian students in both urban and rural areas. Methods: This study's survey items, were developed using items questionnaire that focuses on smoking, eating, socioeconomic status (SES), and social relationships among teenagers aged 14 to 18. The study used two separate, demographically and culturally varied school-based populations. Results: The study found that teenagers in urban areas were more likely to smoke cigarettes, whilst adolescents in rural areas were more likely to practice healthy eating habits. Significant disparities in socioeconomic level (P 0.05) and social interactions (P 0.05) were also discovered, which may have an impact on the health risk behaviours seen in teenagers. The development of focused therapies and public health initiatives to address teenage health risk behaviours will benefit from these findings. Conclusion: More study is necessary to further understand the relationship between risk factors and health behaviours in both urban and rural teenagers in Indonesia. This will make it possible to find efficient preventive techniques and plans adapted to pupils' particular environments and cultural backgrounds.
Co-Authors Aasim, Wan Raihana Wan Ab Latif, Zahidah Ab-Latif, Zahidah Abdul Mutalib, Asila Abdul Mutalib, Asilah Abdullah, Hasmah Anak Gaung, Paul Morisson Che Man, Shaibatul ' Islamiah Che Man, Shaibatul ’ Islamiah Choon, Tan Soo Ismail, Fazhana Jing Rui Tang Johari, Nor Syakina Shaimi, Asma Ulhusna Vionalita, Gisely Wei, Ang Chee