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AKTIVITAS SITOTOKSIK EKSTRAK ETANOL, FRAKSI ETANOL-AIR, ETIL ASETAT SERTA N-HEKSANA BUAH PARE (MOMORDICA CHARANTIA) PADA SEL MCF-7 SECARA IN-VITRO Pamungkas, Adhe Retnantya; Indrayudha, Peni
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 2 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i2.9049

Abstract

Bitter melon is one of fruit that have pharmacological effects such as an anticancer which potentially active on breast cancer cell MCF-7. The aim of this study was to compare the potential cytotoxic activity between ethanol extract of pare and the results of ethanol, ethyl acetate and n-hexane fraction on MCF-7 breast cancer cells and to determine the class of compounds contained in each sample. Pare?s powder was extracted with maceration method in 80% ethanol solvent and then continue to fractionated in 96% ethanol, ethyl acetate and hexane solvent. Extract and fractions were continue to cytotoxic assay using the MTT assay method. Cytotoxic test results showed that the ethanolic extract had no potential cytotoxic activity. Ethyl acetate fraction with the highest concentration 16 µg/mL has the highest potential inhibition 43,87% on MCF-7 cells population. Extract and fractions than continue to phytochemical screening with Thin Layer Chromatography (TLC) method. TLC detection showed that n-hexane fraction contained more compound groups than ethanolic extract, ethanolic-water fraction and ethyl acetate fraction.
The Effect of Snail Mucus (Achatina fulica) on Wound Healing after Tooth Extraction: A Literature Review Ridho, Fiki Muhammad; Pamungkas, Adhe Retnantya; Faisal, Ahmad
Jurnal Ilmu Medis Indonesia Vol. 3 No. 1 (2023): September
Publisher : Penerbit Goodwood

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35912/jimi.v3i1.2320

Abstract

Purpose: Tooth extraction is a frequently performed procedure within the field of dentistry, which results in a wound. The wound healing process can be enhanced by chemical and traditional medicines, one of which is snail mucus, which contains active compounds that play an important role in wound healing process. This study aims to further discuss regarding the effect of snail mucus on wound healing process after tooth extraction. Methodology/approach: This literature review was conducted using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines through PubMed, ProQuest, and Google Scholar database. Results/findings: Snail mucus contains active compounds, such as protein, glycosaminoglycans, metal elements, allantoin, sulfates, calcium, mytimacin-AF, collagen, elastin, glycolic acid, hyaluronic acid, glutathione and vitamins which have effectiveness in enhancing the wound healing process with different mechanisms for each compound, both hemostasis, inflammation, proliferation, and remodeling processes. Limitation: The limitation of this literature review is the lack of high-quality research. Furthermore, the lack of information on the specific doses of snail mucus used and the duration of treatment are critical difficulties that should be addressed in future research to allow for more comprehensive meta-analysis. Additionally, it is required to conduct testing and comparative analysis of the composition of snail mucus across other species in order to ascertain the efficacy of snail mucus content in species other than Achatina fulica. Contribution: The results of this literature review are expected to be a reference in scientific development of wound treatment using traditional medicine, particularly snail mucus. Additionally, it is expected to increase the understanding regarding the effect of snail mucus on wound healing after tooth extraction.