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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Jurnal Penelitian Saintek WARTA Biomedika Pharmacon Majalah Obat Tradisional Journal of Nutraceuticals and Herbal Medicine Martabe : Jurnal Pengabdian Kepada Masyarakat JFIOnline AL MA'ARIEF : JURNAL PENDIDIKAN SOSIAL DAN BUDAYA Jurnal Farmasi Jurnal Farmasi dan Sains Indonesia (JFSI) Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Farmasi Sains dan Praktis Prosiding University Research Colloquium Prosiding Seminar Informasi Kesehatan Nasional Journal of Pharmaceutical and Sciences. Usadha Journal of Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Kefarmasian Indonesia
Peni Indrayudha
Universitas Muhammadiyah Surakarta
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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POLA KUMAN DAN RESISTENSINYA TERHADAP ANTIBIOTIKA DARI SPESIMEN PUS DI RSUD Dr. MOEWARDI TAHUN 2012 Indrayudha, Peni
Pharmacon Vol 13, No 2 (2012)
Publisher : Pharmacon

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.664 KB)

Abstract

Resistensi terhadap antibiotika merupakan problem yang sering terjadi di seluruh dunia termasuk Indonesia. Pola resistensi ini selalu mengalami pergeseran dan perubahan dari setiap periode pemeriksaan. Oleh karena itu perlu suatu usaha untuk mencegah dan mengatasi munculnya resistensi bakteri dengan monitoring pemakaian antibiotika dibidang kesehatan. Penelitian ini bertujuan untuk mengetahui pola kuman dan resistensinya terhadap antibiotika dari spesimen pus di RSUD Dr. Moewardi. Ketepatan penentuan diagnosis dan pemilihan antibiotika berdasarkan uji resistensi sangat membantu dalam penatalaksanaan dan efektifitas terapi. Penelitian dilakukan di Laboratorium Mikrobiologi Klinik RSUD Dr. Moewardi dan Laboratorium Mikrobiologi Kedokteran UNS Surakarta. Spesimen yang digunakan berupa pus (nanah) pasien yang berkunjung atau dirawat di RSUD Dr. Moewardi periode Agustus-Oktober 2012. Jumlah sampel 53, isolasi dan identifikasi dilakukan sesuai standard laboratorium, ditambah dengan data sekunder hasil uji kuman. Uji kepekaan menggunakan metode disc diffusion pada media agar Mueller Hinton. Hasil penelitian menunjukkan dari total sampel yang diisolasi, terdiri dari kuman Gram negatif (66,04%) dan kuman Gram positif (33,96%). Kuman Staphylococcus aureus dominan ditemukan pada spesimen pus (30,19%). Bakteri S. aureus resisten terhadap amoksisilin (93,75%) dan tetrasiklin (87,5%). Kuman Gram negatif Acinetobacter baumanni menunjukkan resistensi tinggi (100%) terhadap siprofloksasin, amoksisilin, gentamisin dan sefotaksim. Kata kunci : Antibiotika, Resistensi, Staphylococcus aureus, Acinetobacter baumanni, pus
FORMULASI KRIM MINYAK ATSIRI RIMPANG TEMU GIRING (Curcuma heyneana Val & Zijp): UJI SIFAT FISIK DAN DAYA ANTIJAMUR TERHADAP Candida albicans SECARA IN VITRO Indrayudha, Peni; Sukmawati, Anita; Rahmawati, Dewi
Majalah Obat Tradisional Vol 15, No 2 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.423 KB) | DOI: 10.14499/mot-TradMedJ15iss2pp%p

Abstract

Minyak atsiri rimpang temu giring (Curcuma heyneana Val & Zijp) berkhasiat sebagai antijamur terhadap Candida albicans dengan kadar hambat minimal (KHM) 0,25% v/v. Sifat minyak atsiri yang mudah menguap menyebabkan daya melekat pada kulit kurang optimal, sehingga perlu diformulasi dalam bentuk krim. Penelitian ini bertujuan untuk mengetahui pengaruh formulasi krim A/M dan M/A minyak atsiri rimpang temu giring terhadap sifat fisik dan daya antijamur terhadap Candida albicans. Minyak atsiri diperoleh dengan destilasi uap dan air (water and steam destillation). Krim dibuat dalam 2 tipe yaitu minyak dalam air (M/A) dan air dalam minyak (A/M) dengan konsentrasi minyak atsiri 10%. krim diuji sifat fisik (viskositas, daya menyebar, kemampuan proteksi, daya melekat, pH), dan uji daya antijamur terhadap Candida albicans secara in vitro. Pengamatan terhadap daya hambat jamur dilakukan setelah diinkubasi selama 48 jam dan diukur diameter zona hambatannya. Analisis data dengan anava satu jalan dilanjutkan uji t-LSD dengan taraf kepercayaan 95 %. Hasil penelitian menunjukkan bahwa krim minyak atsiri rimpang temu giring tipe A/M memiliki viskositas lebih besar daripada tipe M/A. Daya menyebar dan daya proteksi krim M/A lebih baik daripada krim A/M. Daya melekat krim A/M lebih lama daripada krim M/A. Krim M/A dan A/M memiliki pH 6-7. Krim minyak atsiri rimpang temu giring M/A memiliki daya antijamur terhadap Candida albicans yang lebih baik daripada krim A/M, dengan diameter zona hambatan secara berturut-turut 1,84±0,071 cm dan 1,70±0,074 cm.
PEMISAHAN SENYAWA-SENYAWA YANG BERSIFAT SITOTOKSIK TERHADAP SEL MURIN LEUKEMIA P388 DARI EKSTRAK METANOL KULIT BATANG DIPTEROCARPUS CONFERTUS SLOOT (DIPTEROCARPACEAE) Muhtadi, .; Indrayudha, Peni
Biomedika Vol 5, No 1 (2013): Biomedika Februari 2013
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Six compounds, KP-1 up to KP-6 were isolated from the methanol extract of the tree bark of Dipterocarpus confertus Sloot (Dipterocarpaceae). The cytotoxic activities of these compounds were evaluated against murine leukemia P388 cells. Result of these examination indicated that KP-2 compound and KP-1 were very active with each IC 50 value of these compounds were 2.25 and 5.1 μg/mL, respectively. While the others were not active against murine leukemia P388 cells.Keywords: Dipterocarpaceae, Dipterocarpus confertus Sloot, isolated compounds, cytotoxic, and leukemia P-388 cell
PELATIHAN PEMERIKSAAN TAJAM PENGLIHATAN PADA SISWA KELAS 5 SD GEDONGAN I, COLOMADU, KARANGANYAR Sutrisna, Em; Hanwar, Dedi; Indrayudha, Peni; Sujono, Tanti Azizah
WARTA WARTA Volume 10, Nomor 1, Maret 2007
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (24.037 KB) | DOI: 10.23917/warta.v10i1.3210

Abstract

Seeing is crucial aspect in life includes in the field of education. Ifit is not cured the reduction of  sharpness of seeing/visus can affect thestudent ability to study. This action is done to detect  this reduction early.Therefore, the eyes will not be getting worse. The research is done bytraining and demonstrating how to check visus. The location of the researchis SD Gedongan I, Gedongan, Colomadu, Karanganayar. The 31 studentsare checked. The result is that there are 3 students have visus with miop.All the students can participate in this research well.
POLA KUMAN DAN RESISTENSINYA TERHADAP ANTIBIOTIKA DARI SPESIMEN PUS DI RSUD Dr. MOEWARDI TAHUN 2012 Indrayudha, Peni
Pharmacon: Jurnal Farmasi Indonesia Vol 13, No 2 (2012)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.664 KB)

Abstract

Resistensi terhadap antibiotika merupakan problem yang sering terjadi di seluruh dunia termasuk Indonesia. Pola resistensi ini selalu mengalami pergeseran dan perubahan dari setiap periode pemeriksaan. Oleh karena itu perlu suatu usaha untuk mencegah dan mengatasi munculnya resistensi bakteri dengan monitoring pemakaian antibiotika dibidang kesehatan. Penelitian ini bertujuan untuk mengetahui pola kuman dan resistensinya terhadap antibiotika dari spesimen pus di RSUD Dr. Moewardi. Ketepatan penentuan diagnosis dan pemilihan antibiotika berdasarkan uji resistensi sangat membantu dalam penatalaksanaan dan efektifitas terapi. Penelitian dilakukan di Laboratorium Mikrobiologi Klinik RSUD Dr. Moewardi dan Laboratorium Mikrobiologi Kedokteran UNS Surakarta. Spesimen yang digunakan berupa pus (nanah) pasien yang berkunjung atau dirawat di RSUD Dr. Moewardi periode Agustus-Oktober 2012. Jumlah sampel 53, isolasi dan identifikasi dilakukan sesuai standard laboratorium, ditambah dengan data sekunder hasil uji kuman. Uji kepekaan menggunakan metode disc diffusion pada media agar Mueller Hinton. Hasil penelitian menunjukkan dari total sampel yang diisolasi, terdiri dari kuman Gram negatif (66,04%) dan kuman Gram positif (33,96%). Kuman Staphylococcus aureus dominan ditemukan pada spesimen pus (30,19%). Bakteri S. aureus resisten terhadap amoksisilin (93,75%) dan tetrasiklin (87,5%). Kuman Gram negatif Acinetobacter baumanni menunjukkan resistensi tinggi (100%) terhadap siprofloksasin, amoksisilin, gentamisin dan sefotaksim. Kata kunci : Antibiotika, Resistensi, Staphylococcus aureus, Acinetobacter baumanni, pus
UJI AKTIVITAS EKSTRAK DAUN DEWANDARU DAN DAUN KELADI TIKUS TERHADAP PEMOTONGAN DNA SUPERKOIL UNTAI GANDA Indrayudha, Peni; Wijaya, Abdul Rosyid Thoyib; Iravati, Susi
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 3, No 2 (2006)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Keladi tikus (Typhonium flagelliforme (Lodd) BL) is used traditionally as anti cancer and anti virus. Dewandaru (Eugenia uniflora Linn) have known to have antibacterial activity. This experiment was aim to examine whether the extract of Eugenia uniflora Linn and Typhonium flagelliforme (lodd) BL leaves contain protein which had activity to cut double helix supercoiled DNA. The experiment was conduct by incubating DNA plasmid (pUC18) with protein isolated from Eugenia uniflora Linn and Typhonium flagelliforme (lodd) BL leaves extract at room temperature for 1 hour. Crude extracts were made by extraction in 0.14 M NaCl-5 mM sodium phosphate buffer pH 7,2. Extract were considered to have RIPs activity when it was able to cut double helix supercoiled DNA to linear nick circular form. Electrophoretogram was observed under ultraviolet light. Result showed that Typhonium flagelliforme (Lodd) BL) leaves extract contain protein which had activity to cut double helix supercoiled DNA to linear nick circular form. While Eugenia uniflora Linn leaves extract gave negative result.   ABSTRAK Tanaman keladi tikus (Typhonium flagelliforme (Lodd) BL) digunakan sebagai antikanker dan antivirus. Tanaman dewandaru (Eugenia uniflora Linn) telah diketahui memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui apakah ekstak gubal daun dewandaru (Eugenia uniflora Linn) dan daun keladi tikus (Typhonium flagelliforme (lodd) BL) mengandung protein yang mampu memotong DNA superkoil untai ganda. Penelitian ini dilakukan dengan cara menginkubasikan DNA plasmid (pUC18) dengan sejumlah protein dari ekstrak gubal daun Eugenia uniflora Linn dan daun Typhonium flagelliforme (Lodd) BL) pada suhu kamar selama 1 jam. Ekstrak gubal daun dibuat dengan ektraksi daun dalam buffer natrium fosfat 5Mm yang mengandung NaCl 0,14 M Ph 7,2. Ekstrak akan memiliki aktivitas RIPs jika mampu memotong DNA superkoil untai ganda menjadi bentuk nick circular dan linier. Hasil elektroforesis dilihat di bawah sinar UV. Hasil penelitian menunjukkan bahwa ekstrak gubal daun keladi tikus (Typhonium flagelliforme (Lodd) BL) mengandung protein yang mampu memotong DNA superkoil untai ganda menjadi bentuk nick circular dan linier seperti pada RIPs. Sedangkan ekstrak gubal daun dewandaru (Eugenia uniflora Linn) tidak menunjukkan kemampuan memotong DNA superkoil untai ganda menjadi bentuk nick circular dan linier.
Aktivitas Sitotoksik Ekstrak Etanol, Fraksi Etanol, Etil-Asetat, dan Heksana Kulit Jeruk Purut (Citrus hystrix DC.) terhadap Sel Kanker Payudara T47D Nabilla, Isya Isma; Indrayudha, Peni
Pharmacon: Jurnal Farmasi Indonesia Vol 16, No 1 (2019)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/pharmacon.v16i1.8173

Abstract

Kaffir lime peels (Citrus hystric DC.) have been reported that it has activity on HeLa cell. The purpose of this study was to determine the cytotoxic activity of ethanol extract, ethanolic, ethyl acetate, and hexane fraction of kaffir lime peels to T47D breast cancer cells and to identify the compounds of kaffir lime peels (Citus hystrix DC.). Extraction was done by maceration using 96% ethanol. The results of thick extracts were fractionated by the liquid-liquid partition method using solvent with different polarity, namely hexane, ethyl acetate, and ethanol. Cytotoxic activity tests were carried out using the MTT assay. Identification of compounds were carried out by Thin Layer Chromatography (TLC) method with silica gel GF 254 as a stationary phase. The mobile phase used was hexane:ethylacetate (6:4) for ethanol extract, ethyl acetate and hexane fractions, and hexane:ethylacetate (4:6) for ethanol fractions. IC50 ethanol extract, ethyl acetate and hexane fractions obtained were 268.49, 286.82, and 136.27 mg/mL respectively. Ethanol extract, ethyl acetate, and hexane fractions contain alkaloid, flavonoid, terpenoid and phenolic compounds, while the ethanol fraction contains only flavonoid compound. Cytotoxic results of ethanol extract, ethanol, ethyl acetate, and hexane fraction of kaffir lime peels did not active against T47D breast cancer cells.
PEMISAHAN SENYAWA-SENYAWA YANG BERSIFAT SITOTOKSIK TERHADAP SEL MURIN LEUKEMIA P388 DARI EKSTRAK METANOL KULIT BATANG DIPTEROCARPUS CONFERTUS SLOOT (DIPTEROCARPACEAE) Muhtadi, .; Indrayudha, Peni
Biomedika Vol 5, No 1 (2013): Biomedika Februari 2013
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v5i1.269

Abstract

Six compounds, KP-1 up to KP-6 were isolated from the methanol extract of the tree bark of Dipterocarpus confertus Sloot (Dipterocarpaceae). The cytotoxic activities of these compounds were evaluated against murine leukemia P388 cells. Result of these examination indicated that KP-2 compound and KP-1 were very active with each IC 50 value of these compounds were 2.25 and 5.1 ?g/mL, respectively. While the others were not active against murine leukemia P388 cells.Keywords: Dipterocarpaceae, Dipterocarpus confertus Sloot, isolated compounds, cytotoxic, and leukemia P-388 cell
UJI AKTIVITAS EKSTRAK DAUN DEWANDARU DAN DAUN KELADI TIKUS TERHADAP PEMOTONGAN DNA SUPERKOIL UNTAI GANDA Indrayudha, Peni; Wijaya, Abdul Rosyid Thoyib; Iravati, Susi
Jurnal Farmasi Indonesia Vol 3, No 2 (2006)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v3i2.85

Abstract

Keladi tikus (Typhonium flagelliforme (Lodd) BL) is used traditionally as anti cancer and anti virus. Dewandaru (Eugenia uniflora Linn) have known to have antibacterial activity. This experiment was aim to examine whether the extract of Eugenia uniflora Linn and Typhonium flagelliforme (lodd) BL leaves contain protein which had activity to cut double helix supercoiled DNA. The experiment was conduct by incubating DNA plasmid (pUC18) with protein isolated from Eugenia uniflora Linn and Typhonium flagelliforme (lodd) BL leaves extract at room temperature for 1 hour. Crude extracts were made by extraction in 0.14 M NaCl-5 mM sodium phosphate buffer pH 7,2. Extract were considered to have RIPs activity when it was able to cut double helix supercoiled DNA to linear nick circular form. Electrophoretogram was observed under ultraviolet light. Result showed that Typhonium flagelliforme (Lodd) BL) leaves extract contain protein which had activity to cut double helix supercoiled DNA to linear nick circular form. While Eugenia uniflora Linn leaves extract gave negative result.   ABSTRAK Tanaman keladi tikus (Typhonium flagelliforme (Lodd) BL) digunakan sebagai antikanker dan antivirus. Tanaman dewandaru (Eugenia uniflora Linn) telah diketahui memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui apakah ekstak gubal daun dewandaru (Eugenia uniflora Linn) dan daun keladi tikus (Typhonium flagelliforme (lodd) BL) mengandung protein yang mampu memotong DNA superkoil untai ganda. Penelitian ini dilakukan dengan cara menginkubasikan DNA plasmid (pUC18) dengan sejumlah protein dari ekstrak gubal daun Eugenia uniflora Linn dan daun Typhonium flagelliforme (Lodd) BL) pada suhu kamar selama 1 jam. Ekstrak gubal daun dibuat dengan ektraksi daun dalam buffer natrium fosfat 5Mm yang mengandung NaCl 0,14 M Ph 7,2. Ekstrak akan memiliki aktivitas RIPs jika mampu memotong DNA superkoil untai ganda menjadi bentuk nick circular dan linier. Hasil elektroforesis dilihat di bawah sinar UV. Hasil penelitian menunjukkan bahwa ekstrak gubal daun keladi tikus (Typhonium flagelliforme (Lodd) BL) mengandung protein yang mampu memotong DNA superkoil untai ganda menjadi bentuk nick circular dan linier seperti pada RIPs. Sedangkan ekstrak gubal daun dewandaru (Eugenia uniflora Linn) tidak menunjukkan kemampuan memotong DNA superkoil untai ganda menjadi bentuk nick circular dan linier.
FORMULASI SUSPENSI DOKSISIKLIN MENGGUNAKAN SUSPENDING AGENT PULVIS GUMMI ARABICI: UJI STABILITAS FISIK DAN DAYA ANTIBAKTERI Chasanah, Nur; Trisharyanti DK, Ika; Indrayudha, Peni
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol 1, No 2 (2011): Oktober 2010 - Maret 2011
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (430.779 KB) | DOI: 10.22219/far.v1i2.1165

Abstract

Suspension is a dosage contain solid drug material with insoluble form and smooth. Doxycycline is an antibiotic which difficult dissolve in water. Stable doxycycline suspension during 2 week if it kept at space temperature, stable on the air but will change to be dark if it influence sunshine. The aim of the research to evaluate physical stability and ability antibacterial stability doxycycline suspension with PGA as suspending agent. Research category are: Independent variable : concentration of suspending agent (PGA), suspension holding time. Dependent variable: physical stability (sedimentation volume, viscosity, redispersibility, easy to pour, and particle size) and antibacterial of doxycycline suspension, and variabel control is temperature suspension making, create of suspension. Data collecting with measuring physical stability and ability antibacterial of doxycycline suspension. The result shows doxycycline suspension with suspending agent PGA had physical stability and antibacterial ability is good. Physical Stability test show  formula 1 rate PGA 5% is formula had physical stability better than formula 2 with rate PGA 7,5% and formula 3 withrate PGA 10%. stability ability test antibacterial show formula 2 is the  best in pursues Staphylococcus aureus.
Co-Authors . MUHTADI . Muhtadi . Sismindari abdul, ahwan Adhe Retnantya Pamungkas Ahmad Farras Musyayaf Andi Suhendi Andika Andika Anita Sukmawati Anna Fitriawati Arini Nur Yunia Puspitaningrum Rahmwati Aryani, Diya Cita Hanif Muflihah Dedi Hanwar Dewi Rahmawati DEWI RAHMAWATI Em Sutrisna Firstca Aulia Rachma Haryoto Haryoto Haryoto Haryoto Haryoto Hidayah Kurniawati Ika Trisharyanti Dian Kusumowati Isya Isma Nabilla Joko Kristianto Muhammad Ajmal Shah Muhtadi Muhtadi Nabila, Devina Talitha Nabilla, Isya Isma Nastiti Wijayanti Nissa Ainni, Ayu Nova Lovena, Tri Nur Chasanah Nuri Utami Nursalinda Kusumawati Nurul Fadel, M. Pamungkas, Adhe Retnantya Pangesti, Adel Hening Prayitno, Imam Purwaningsih - Putri Agustina Rima Munawaroh Rossida , Salsabilla Aulya Saroyobudiyono, Haryoto Susi Iravati Tanti Azizah Tanti Azizah Sujono Tri Oktarina Tri Yulianti Tri Yulianti, Tri Wijaya, Abdul Rosyid Thoyib Wijaya, Abdul Rosyid Thoyib Wijianto, Diski Wahyu Yanuar, Rizqi Fitria Zakiyatul Fadhilah