Article Per Year (5 Year)
p-Index From 2020 - 2025
0.61
P-Index
This Author published in this journals
All Journal JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pendidikan, Psikologi Dan Kesehatan (J-P3K) Jurnal Res Justitia : Jurnal Ilmu Hukum Sepakat : Jurnal Pastoral Kateketik Annales Bogorienses
IMELDA, MARIA
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Nursing Public Health Social Sciences Other

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 8 Documents
Search

 1 
PERTUMBUHAN BIAK JARINGAN DENDROBIUM SCHULLERI PADA TIGA MACAM MEDIA GANDAWIDJAJA, DJUNAEDI; IMELDA, MARIA
BERITA BIOLOGI Vol 2, No 8 (1984)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v2i8.1413

Abstract

Teknik biakjaringan telah banyak dimanfaatkan baik untuk tujuan perbanyakan tanaman rnaupun untuk tujuan lain. Penerapan teknik ini dalam bidang peranggrekan terutama diarahkan pada tujuan komersial. Pada umumnya anggrek mudah diperbanyak dengan teknik biak jaringan, tetapijenis jenis tertentu ataupun bagian-bagian tertentu dari tanaman anggrek kadang-kadang sulit ditumbuhkan dalam suatu medium buatan (Mitra 1971, Sagawa dan Shoji 1967, Stewart dan Button 1975).Pemilihan bahan biak dan medium sangat menen+ukan berhasil tidaknya suatu biak jaringan (Gamborg et at 1976).Dalam usaha merangsang pertumbuhanbiak, berbagai macam hormon ataupun bahan organik, misalnya air kelapa, telah sering digunakan. Percobaan ini dilakukan dalam rangka mencari medium yang cocok bagi pertumbuhan biak jaringan Dendrobium schulleri J.J.S. sebagai alternatif cara perbanyakan dan pelestariannya. Pemilihan D. schulleri sebagai bahan percobaan didasarkan atas pertimbangan potensinya yang tinggi sebagai tanaman hias penghasil bunga potong. Pertimbangar lainnya ialah bahwa jenis yang asli dari Indonesia ini sudah banyak terkuras dan terdesak dari habitat aslinya sehingga dikhawatirkan akan mengalami erosi populasi ataupun erosi genetik.
INDUKSI MUTASI KULTUR IN VITRO Amorphophallus muelleri Blume DENGAN IRRADIASI GAMMA Poerba, Yuyu S; IMELDA, MARIA; WULANSARI, AIDA; MARTANTI, DIYAH
Jurnal Teknologi Lingkungan Vol. 10 No. 3 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.166 KB) | DOI: 10.29122/jtl.v10i3.1482

Abstract

Amorphophallus muelleri Blume (Araceae) is valued for its glucoman content for use infood industry (heathy diet food), paper industry, pharmacy and cosmetics. The cultivationof A. muelleri is hampered by limited genetic quality of seed. The species is triploid(2n=3x=39), the seed is developed apomictically, and pollen production is low. Thespecies is only propagated vegetatively. This may explain that the species is difficultto breed conventionally and genetic variability in the existing landraces cultivars israther limited. Conservation of this species, therefore, is important for availability of thespecies in the future use of this plant. The objective of present research is to increasegenetic variation by induce mutation using gamma-rays irradiation of shoot culturesof A. muelleri and to identify DNA polymorphism induced by gamma irradiation usingrandom amplified polymorphic DNA (RAPD), so the mutants produced can be used forbreeding purposes and for conservation program. Results of the experiment showedthat gamma irradiation less than 5 gray was effective to induce mutation of A. muelleri.Four RAPD primers generated 35 scorable bands with 100% polymorphic bands. Sizeof the bands varied from 350bp to 2.0kbp. Clustering analysis was performed based onRAPD profiles using the UPGMA method. The range of genetic distance among individualgenotypes was from from 0.00 to 0.72, while genetic variance of the population was0.21 + 0.13. The eighteen genotypes were proof to be mutants. The mutants producedin this experiment could be used as new germplasms for breeding purposes as well asfor use in conservation strategy
PERBANYAKAN IN VITRO PISANG KEPOK var. UNTI SAYANG TAHAN PENYAKIT DARAH MELALUI PROLIFERASI TUNAS Imelda, Maria; Wulansari, Aida; Sari, Laela
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1039.111 KB) | DOI: 10.29122/jbbi.v5i1.2626

Abstract

In Vitro Propagation of Kepok Banana var. Unti Sayang Resistant to Blood Disease through Shoot ProliferationABSTRACTKepok is a popular banana variety but sensitive to blood disease caused by Ralstonia solanacearum (Smith). The discovery of a natural mutant of Kepok banana var. Unti Sayang from Sulawesi which male bud falls naturally, is a shortcut to bypass the chains of the spread of blood disease, since the disease is transmitted by insects through the wounds of the male buds. The superior mutant needs to be mass propagated and disseminated to endemic areas to inhibit the spread of blood disease. To achieve that goal, an efficient and effective techniques of in vitro shoot proliferation needs to be developed. Shoot proliferation was performed by addition of BAP, thidiazuron and adenine sulphate. The results showed that the best medium for shoot multiplication was B2T5A (MS+2 mg/L BAP+0,5 mg/L TDZ+20 mg/L adenine sulphate), and for shoot growth was B4A (MS+4 mg/L BAP+20 mg/L adenine sulphate). Rooting was induced on MS medium without hormones. Acclimatization of plantlets on mixed soil, compost and husks with a ratio of 1:1:1 resulted in 92,35% survival rate.Keywords: blood disease, in vitro shoot,  male budless, natural mutant, var. Unti Sayang  ABSTRAKPisang kepok merupakan varietas yang digemari tetapi sangat peka terhadap penyakit darah yang ditimbulkan oleh bakteri Ralstonia solanacearum (Smith). Ditemukannya mutan alami pisang kepok yang jantungnya gugur secara alami yaitu varietas Unti Sayang dari Sulawesi, merupakan jalan pintas untuk memotong rantai penyebaran penyakit darah, mengingat penyakit ini ditularkan oleh serangga melalui luka bekas bunga jantan pada jantung. Mutan unggul tersebut perlu diperbanyak secara massal dan disebarluaskan ke daerah endemik untuk menghambat penyebaran penyakit darah. Untuk mencapai tujuan tersebut, perlu dikembangkan teknik perbanyakan in vitro pisang kepok Unti Sayang yang efektif dan efisien melalui proliferasi tunas. Proliferasi tunas dilakukan dengan penambahan BAP, thidiazuron dan adenin sulfat. Hasil penelitian ini menunjukkan bahwa media terbaik untuk multiplikasi tunas adalah B2T5A (MS+2 mg/L BAP+0,5 mg/L TDZ+20 mg/L adenin sulfat), media terbaik untuk pertumbuhan tunas adalah B4A (MS+4 mg/L BAP+20 mg/L adenin sulfat). Akar dapat diinduksi pada media MS tanpa hormon. Aklimatisasi planlet pada media campuran tanah, kompos dan sekam dengan perbandingan 1:1:1 menghasilkan 92,35% planlet hidup.Kata Kunci: penyakit darah, tunas in vitro, tanpa jantung, mutan alami, var. Unti Sayang 
ANALISIS PENERAPAN POLITIK UANG PADA MASA DEMOKRASI DI KOTA SERANG PROVINSI BANTEN Zahra, Maitza; Imelda, Maria; Ar-Rifdah, Nabila; Nurul, Reysha; Muhammad, Athhar
Jurnal Res Justitia: Jurnal Ilmu Hukum Vol. 4 No. 1 (2024): Jurnal Res Justitia : Jurnal Ilmu Hukum
Publisher : LPPM Universitas Bina Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46306/rj.v4i1.103

Abstract

The politics of money politic is not a foreign thing to be discovered. Many parts of Indonesia use the political lifeline of money during elections to attract people, as this article focuses on the city of attack, the province of banten. This is because of the poor quality of human resources and the weak economic growth in Indonesia. The politics of money also has many kinds so it can be distinguished according to its purposes and meanings. Politics of money also has differing meanings according to experts, so we examined the opinion of the experts in results and discussions
Pemberian Konseling Kelompok Sebagai Upaya Meningkatkan Empati Siswa Berperilaku Bullying di SMP Negeri Alok Imelda, Maria; Nei, Florensia
JURNAL PENELITIAN PENDIDIKAN, PSIKOLOGI DAN KESEHATAN (J-P3K) Vol 6, No 1 (2025): J-P3K
Publisher : Yayasan Mata Pena Madani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51849/j-p3k.v6i1.637

Abstract

Penelitian ini bertujuan untuk mengetahui tingkat perilaku bullying dan empati pada siswa SMP Negeri Alok. Bullying merupakan fenomena yang sering terjadi dalam lingkungan sekolah. Bullying atau perundungan lebih melibatkan para remaja sebagai pelaku terjadinya bullying. Pada peneltiain ini menggunakan metode penelitian kuantitatif dengan pengujian validitas dan reliabilitas serta penentuan kategori perilaku bullying dan kategori empati. Penelitian ini juga menggunakan metode assessment berupa wawancara dan observasi pada guru BP/BK dan para siswa SMP Negeri Alok. Melalui hasil assessment yang dilakukan maka, pemberian intervensi di lakukan yaitu berupa konseling kelompk dengan pendekatan client centered pada para pelaku bullying dan dilakukan dengan tiga sesi konseling kelompok. Dan konseling kelompok dengan pendekatan client centered terbukti efektif dalam meminimalisir perilaku bullying dan meningkatkan empati. Hal ini ditandai dengan kemauan para siswa yang diberi konseling untuk membuat komitmen dalam mengurangi perilaku bullying dan ingin berteman baik dengan seluruh anggota kelas dikarenakan para siswa telah mengetahui konsekuensi yang mereka terima ketika mereka melakukan bullying dan dengan konseling kelompok diharapakn agar para siswa memiliki sikap empati yang besar bagi teman sebayanya.
Gereja sebagai Agen Transformasi Ekologis: Studi tentang Peran Gereja dalam Menjaga Lingkungan Hidup berdasarkan Laudato Si’ Crespany, Vinsensia Yulisa; Imelda, Maria
Sepakat : Jurnal Pastoral Kateketik Vol. 11 No. 2 (2025): September : Sepakat : Jurnal Pastoral Kateketik
Publisher : Sekolah Tinggi Pastoral Tahasak Danum Pambelum Keuskupan Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58374/sepakat.v11i2.448

Abstract

Krisis ekologis telah menarik perhatian besar di seluruh dunia. Gereja juga telah menyerukan tindakan nyata. Melalui ensiklik yang ditulis oleh Paus Fransiskus, disampaikan ajakan untuk melakukan transformasi positif sebagai tanggapan terhadap krisis ekologis. Penelitian ini bertujuan untuk mengkaji peran Gereja sebagai agen transformasi serta mengimplementasikan pemahaman atas Laudato si’ dalam tindakan konkret. Metode yang digunakan adalah studi pustaka dengan pendekatan deskriptif kualitatif, menelaah dokumen Gereja seperti Laudato si’ dan data sekunder lainnya. Hasil penelitian ini menunjukkan bahwa Gereja merupakan agen transformasi ekologis yang memiliki peran penting dalam memajukan kesejahteraan ekologis. Dalam Laudato si’, terdapat tiga aspek yang membentuk implikasi peran Gereja: pertobatan ekologis, liturgi, dan pendidikan iman, yang menumbuhkan kesadaran ekologis serta solidaritas terhadap para korban krisis ekologis. Gereja tidak hanya menjadi pembawa pesan, tetapi juga secara aktif berperan sebagai penggerak perubahan berkelanjutan.
In Vitro Propagation of Buah Merah (Pandanus Conoideus Lam) Through Lateral Bud Proliferation Imelda, Maria; Wulansari, Aida; Sumarnie, Sumarnie
Annales Bogorienses Vol. 12 No. 1 (2008): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pandanus conoideus Lam or 'Buah merah' of the Pandanaceae is a native to East Indonesia, particularly Papua and North Maluku. Traditionally the fruits are used for health promotion and maintenance as well as for curing several illnesses. Recently, it has been reported that the fruit its are potential for cancer medication. As a result, there has been an overexploitation of the plants from their habitats. In order to anticipate their possible disappearance due to overexploitation in the wild, an efficient and effective technology for the mass propagation, conservation, and cultivation of these plant should be developed. Generally buah merah is propagated vegetatively by offshoots and stem cuttings or generatively by seeds. Micropropagation has many advantages over the conventional method because the technique allows mass clonal and pathogen-free production of plants at a high rate of multiplication all year round. In this research the effects of 0.1-0.2 mg/L thidiazuron (TDZ), 0.5-1.0 mg/L benzyl amino purine (BAP), and 0.25-0.5 mg/L kinetin (KN) on shoot bud induction and proliferation of P. conoideus were investigated using nodal infections or lateral buds of P. conoideus on modified Murashige and Skoog (MS) medium. Shoots were rooted on MS medium without plant growth regulator (PGR). The result showed that lateral bud of Pandanus start d to initiate growth after 4-7 days in culture. The best medium for shoot proliferation was MS containing either 0.5 mg/l BAP with 0.1 mg/l TDZ or 1 mg/l BAP with 0.5 mg/l KN giving a multiplication rate of 16.5 shootlets per shoot bud explant after 8 weeks. Rooting of shoots was successfully conducted on MS medium without PGR. Acclimatization of rooted plantlets was achieved on a mixed medium of cocopeat and soil (1:1).
Propagation of Sukun (Artocarpus altilis (Parkinson) Fosberg) Through In Vitro Shoot Proliferation Imelda, Maria; Wulansari, Aida; Sari, Laela
Annales Bogorienses Vol. 13 No. 1 (2009): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Sukun (Artocarpus altilis (Parkinson) Fosberg) of the Moraceaae is a big tree which can grow to 15-20 m in height and native to the Asia-Pasific region. Beside its delicious fruit, sukun is also known as a traditional herbal medicinal plant in the region, including Indonesia. Nearly all parts of the plant, such as roots, stems and leaves are believed by local communities to be capable of curing liver disease, hypertension, cardiac arrest, toothache, renal problem and even skin itchiness. The collaborative research between LIPI and PR China, on developing herbal medicines indicated that sukun has a great potential for treating cardiovascular disease. However, the availability of raw materials still poses a big constraint for the industry of herbal medicines. Generally, sukun is propagated by root or stem cuttings, since in Indonesia sukun does not produce any seeds. However such method only produces limited planting materials. In general tissue culture propagated plants have many advantages, namely being clonal, free from pest and diseases, more uniform, and allowing a high rate of plant multiplication. Therefore, the technique for sukun propagation has been developed by the LIPI Research Centre for Biotechnology. In this research the effects of 1-5 mg/l benzyl amino purine (BAP) and 20-40 mg/l adenine sulphate (AS)on shoot bud proliferation were investigated using lateral shoot buds on a modified Murashige and Skoog (MS) medium with addition of 150 ml/l coconut water (CW). Shoots were rooted on MS medium without plant growth regulators (PGRs). The results showed that the best medium for in vitro shoot proliferation was a modified MS medium containing 2 mg/l BAP, 40 mg/l AS and 150 ml/l CW. The best medium for rooting is MS medium containing 1 mg indole butyric acid (IBA), producing roots within 3 weeks.
Co-Authors Ar-Rifdah, Nabila Crespany, Vinsensia Yulisa Diyah Martanti, Diyah Gandawidjaja, Djunaedi laela Sari, laela Muhammad, Athhar Nei, Florensia Nurul, Reysha Sumarnie, Sumarnie Wulansari, Aida Yuyu S Poerba Zahra, Maitza