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All Journal INDONESIAN JOURNAL OF PHARMACY Jurnal Pendidikan Kimia (JPKim) Jurnal Ilmiah Agroust
Triono, Sugeng
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Medicine & Pharmacology

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Effect of Growth Factor In Callus Induction and Bioactive Compounds In Seed Explant of Kaffir Lime (Citrus hystrix DC.) Tunjung, Woro Anindito Sri; Fatonah, Vita; Christy, Ghea Putri; Triono, Sugeng; Hidayati, Lisna
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp61

Abstract

Our previous study showed that kaffir lime leaf extracts may have anti-cancer properties. However, production of  bioactive compounds is affected by environmental factors. Here, we present a method to control environmental conditions using in vitro culture techniques such as callus induction. Calluses were induced from seed embryo explants of kaffir lime on MS medium with combinations of 2,4-D and BAP at concentrations 1:0.5; 1:1; and 2:1, respectively. Fourty and 60 days-old calluses were extracted using chloroform and ethyl acetate and analyzed by GC-MS. Results showed all combinations of 2,4-D and BAP were able to induce callogenesis from seed embryo explants of kaffir lime with no significant differences of callus initiation time, biomass, morphology and growth rates. However differences were detected in the bioactive compound profiles. In kaffir lime callus, both fatty acids and secondary metabolites were detected. Specifically, in 40 days-old calluses (exponential growth phase) we detected α-pinene and 1.8–cineole in plants treated with 2,4-D: BAP at concentration 1:0.5 and 2:1. In 60 days-old calluses (stationary phase) we detected a number of compounds in plants treated with 2,4-D:BAP at concentrations of 1:0.5 and 2:1, including caryophyllene, linoleoyl chloride, thiogeraniol, stigmasterol, clianosterol, citronellal, neo-isopulegol, citronellol, geraniol, eugenol, cyclopropane, pristane, elemol and farnesol
PENGARUH WAKTU PEMBERIAN DAN DOSIS PUPUK MAJEMUK TERHADAP PERTUMBUHAN DAN HASIL TANAMAN PADI MERAH (Oryza Nivara L.) VARIETAS INPARI 24 GABUSAN Triono, Sugeng; Maryani, Yekti; Pamungkas, Djoko Heru
JURNAL ILMIAH AGROUST Vol 5 No 2 (2021): Jurnal Ilmiah Agroust
Publisher : Universitas Sarjanawiyata Tamansiswa

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Penelitian ini bertujuan untuk mengetahui pengaruh waktu pemberian dan dosis pupuk NPK phonska terhadap pertumbuhan dan hasil padi beras merah varietas inpari 24 gabusan. Penelitian ini dilakukan di Pusat Pelatihan Pertanian dan Pedesaan Swadaya (PAS) “Lestari Makmur”, Jalan Wates Km. 12 Dusun Semampir, Desa Argorejo, Kecamatan Sedayu, Kabupaten Bantul Yogyakarta selama kurang lebih 90 hari. Dengan ketinggian tempat 88 mdpl, jenis tanah regosol, dengan pH tanah 5,5-7, suhu rata-rata yaitu 26-32oC, dan curah hujan yaitu 1.654 mm/tahun. Penelitian dilakukan dengan percobaan faktorial 3 x 3 dengan menggunakan rancangan acak kelompok lengkap (RAKL) meliputi 2 faktor yaitu waktu pemberian pupuk yang terdiri dari 3 level yaitu: 14 dan 35 HST (Z1), 14 dan 50 HST (Z2), 14, 35 dan 50 HST (Z3). Faktor kedua adalah dosis pupuk yang terdiri dari 3 level meliputi: 300 kg per ha (S1), 400 kg per ha(S2), 500 kg per ha (S3). Analisis data dengan menggunakan sidik ragam dengan menggunakan Uji Jarak Berganda Duncan (DMRT) Duncan Multiple Range Test pada taraf α = 5%. Hasil penelitian dapat disimpulkan bahwa Perlakuan waktu pemberian dan dosis pupuk NPK menunjukkan tidak ada interaksi terhadap semua variabel pertumbuhan dan hasil padi merah inpari 24, Perlakuan waktu pemberian pupuk NPK menunjukkan tidak berpengaruh terhadap pertumbuhan dan hasil tanaman, Perlakuan dengan dosis pupuk NPK menunjukkan tidak berpengaruh terhadap pertumbuhan dan hasil tanaman.
Synthesis of (3E,5E)-1-benzil-3,5 bis (3 (benziloksi)benziliden)piperidin-4-on curcumin analogues and their potential as breast anticancer agents: Assessment using MTT test and molecular docking Astuti, Endang; Kultsum, Jihan Alfiyah; Aulia, Zarah; Rahmawari, Frika; Gurning, Kasta; Triono, Sugeng; Haryadi, Winarto; Pranowo, Harno Dwi
Jurnal Pendidikan Kimia Vol. 16 No. 3 (2024): J. Pendidik. Kim : December 2024
Publisher : Pascasarjana Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jpkim.v16i3.65150

Abstract

Breast cancer is a serious disease that occurs in women and contributes to the highest mortality compared to other types of cancer. This study aims to synthesize curcumin analog compounds ((3E,5E)-1-benzyl-3,5 bis (3 (benzyloxy)benzylidene) piperidin-4-one), test them in vitro against various breast cancer cells (T47D, HER-2, MCF-7, and 4T1) and normal cells (vero cells), and study their molecular docking. Synthesis was carried out by reacting 3-benzyloxybenzaldehyde with N-benzyl-4-piperidone catalyzed by 5% KOH at room temperature; in vitro testing was carried out using the Microculture Tetrazolium Technique Assay method, ADMET analysis with an online database server, and molecular docking studies in Autodoc Vina. The synthesis results obtained yellow solid powder with a yield of 65.85%, characterization with TLC gave black fluorescence (Rf 0.63), melting point 114-116oC, TLC scanner one peak (100%), retention time 0.65 minutes, 1H &13C-NMR analysis showed the molecular formula C40H35NO3, moderate activity against 4T1 breast cancer cells and inactivity on T47D, HER-2, and MCF-7 cancer cells, and did not show cytotoxicity to normal cells (vero cells). ADMET predictions from Lipinski's five rules contained two parameters that did not meet, namely molecular weight and log P value. Molecular docking studies were carried out on estrogen receptor protein (ER)-α (PDB ID: 2ERT), which showed a binding affinity energy of -8.7 kcal/mol and -7.1 kcal/mol of the native ligand. Further research and development is needed on synthetic curcumin analog compounds to increase their activity value against breast cancer by paying attention to Lipinski's five rules to obtain compounds with better potential activity and ADMET.
Co-Authors Aulia, Zarah Christy, Ghea Putri Djoko Heru Pamungkas Endang Astuti Fatonah, Vita Harno Dwi Pranowo Kasta Gurning Kultsum, Jihan Alfiyah Lisna Hidayati Rahmawari, Frika Winarto Haryadi Woro Anindito Sri Tunjung Yekti Maryani