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All Journal Bioma : Berkala Ilmiah Biologi Journal of Mathematical and Fundamental Sciences Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology INDONESIAN JOURNAL OF PHARMACY Majalah Farmaseutik Berkala Penelitian Hayati

Woro Anindito Sri Tunjung

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Author-ID : 3096304

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics

Published : 9 Documents Claim Missing Document

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Articles

Effect of Growth Factor In Callus Induction and Bioactive Compounds In Seed Explant of Kaffir Lime (Citrus hystrix DC.) Tunjung, Woro Anindito Sri; Fatonah, Vita; Christy, Ghea Putri; Triono, Sugeng; Hidayati, Lisna
Indonesian Journal of Pharmacy Vol 31 No 2, 2020
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm31iss2pp61

Our previous study showed that kaffir lime leaf extracts may have anti-cancer properties. However, production of bioactive compounds is affected by environmental factors. Here, we present a method to control environmental conditions using in vitro culture techniques such as callus induction. Calluses were induced from seed embryo explants of kaffir lime on MS medium with combinations of 2,4-D and BAP at concentrations 1:0.5; 1:1; and 2:1, respectively. Fourty and 60 days-old calluses were extracted using chloroform and ethyl acetate and analyzed by GC-MS. Results showed all combinations of 2,4-D and BAP were able to induce callogenesis from seed embryo explants of kaffir lime with no significant differences of callus initiation time, biomass, morphology and growth rates. However differences were detected in the bioactive compound profiles. In kaffir lime callus, both fatty acids and secondary metabolites were detected. Specifically, in 40 days-old calluses (exponential growth phase) we detected α-pinene and 1.8–cineole in plants treated with 2,4-D: BAP at concentration 1:0.5 and 2:1. In 60 days-old calluses (stationary phase) we detected a number of compounds in plants treated with 2,4-D:BAP at concentrations of 1:0.5 and 2:1, including caryophyllene, linoleoyl chloride, thiogeraniol, stigmasterol, clianosterol, citronellal, neo-isopulegol, citronellol, geraniol, eugenol, cyclopropane, pristane, elemol and farnesol

Black Rice Bran Extracts and Fractions Containing Cyanidin 3-glucoside and Peonidin 3-glucoside Induce Apoptosis in Human Cervical Cancer Cells Rarastoeti Pratiwi; Woro Anindito Sri Tunjung; R. Rumiyati; Alfi Rizqi Amalia
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Anthocyanin of pigmented rice inhibits the growth of cancer cells. The cytotoxicity and apoptosisinducing properties of local black rice (cv Cempo Ireng) extracts and fractions, which contain anthocyaninincluding cyanidin 3-glucoside and peonidin 3-glucoside, on human cervical cancer cell line (HeLa cells) hasbeen evaluated. The pigmented rice bran was extracted and fractionated using methanol-HCl. The MTT testwas performed on HeLa cell cultures to observe the IC50 value. Preparative TLC was performed to obtain thefractions of black rice bran. Cyanidin 3-glucoside and peonidin 3-glucoside were identified in the pigmentedrice bran extract and fractions using UHPLC. Flowcytometry analysis was performed to measure the percentageof apoptotic cells. Our results suggest that the fractions are more toxic than the methanolic crude extract withIC50 values of 85.95 ± 5.56 μg/mL (the lowest one) and 408.13 ± 51.9 μg/mL, respectively. The concentration ofcyanidin 3-glucoside and peonidin 3-glucoside in the methanolic extract were 1.89 and 0.84 μg/mg, respectively.The apoptosis induction by fractions F2 and F4 (52 and 55%) were significantly higher compared to fractionF3 and F5 (30 and 33%) and doxorubicin (21%). Cyanidin 3-glucoside was detected in F4 (0.14 μg/ml) whilepeonidin 3-glucoside in F2 (0.012 μg/ml), however both were not detected in F3 and F5.

Identification of BSA B1 Bacteria and Its Potency of Purified Cellulase to Hydrolyze Chlorella zofingiensis Rifqi Zahroh Janatunaim; Radhiyah Mardhiyah Hamid; Ghea Putri Christy; Yekti Asih Purwestri; Woro Anindito Sri Tunjung
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Cellulase has been widely used as biocatalyst in industries. Production of cellulase from microorganismshas many advantages such as short production time and less expense. Our previous study indicated that oneof cellulolytic bacteria from digestive tract of milkfish (Chanos chanos), namely BSA B1, showed the highestcellulase activity. The objective of this study was to determine the phylogenetic of BSA B1 strain using 16SrRNA gene sequence. Furthermore, this study also determine the specific activity of purified cellulase from BSAB1 strain and its potency to hydrolyze Chlorella zofingiensis cellulose. Cellulase was purified using ammoniumsulphate precipitation, dialysis, and ion exchange chromatography. The purified cellulase was used to hydrolyzecellulose of C. zofingiensis. The result demonstrated that BSA B1 strain was closely related with Bacillus aeriusand Bacillus licheniformis. The specific activity of the crude enzyme was 1.543 U mL-1; after dialysis was 4.384 UmL-1; and after chromatography was 7.543 U mL-1. Purified cellulase exhibited activity in hydrolyzed both CMCand C. zofingiensis. Compared to commercial cellulase, purified cellulase had lower activity in hydrolyzed CMCbut higher activity in hydrolyzed C. zofingiensis. Ethanol dehydration could potentially increase the reducingsugar yield in cellulose hydrolysis when used appropriately. Morphology of C. zofingiensis cell has changedafter incubation with cellulases and ethanol dehydration indicated degradation of cell wall.

Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia Pratiwi Apridamayanti; Rarastoeti Pratiwi; Yekti Asih Purwestri; Woro Anindito Sri Tunjung; Rumiyati Rumiyati
Indonesian Journal of Biotechnology Vol 22, No 1 (2017)
Publisher : Universitas Gadjah Mada

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.

Growth of Kaffir Lime (Citrus hystrix DC) Cell Line Derived from Seed Explant After Yeast Elicitation Using Pure and Technical Grade Yeast Dewi Yuliana Rizqi; Frisca Damayanti; Fhea Putri Cristy; Alisa Julia Nurulita; Aries Bagus Sasongko; Endang Semiarti; Woro Anindito Sri Tunjung
Journal of Tropical Biodiversity and Biotechnology Vol 7, No 1 (2022): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.68650

The addition of elicitors in kaffir lime (Citrus hystrix DC.) culture is one of strategies for obtaining and increasing the production of secondary metabolites. Saccharomyces cerevisiae is one of the elicitors that can be used to increase secondary metabolites such as terpenoids. However, in its use, the pure cultures of S. cerevisiae are expensive. Therefore, the first objective of this study was to analyze the ability of technical grade (commercial baker’s yeast) to be used as an elicitor and measure the growth of kaffir lime cell line after being elicited by pure and technical grade (commercial baker’s yeast). The second objective is to determine the best time to subculture kaffir lime cell line after elicitation. We observed the morphology and measured the growth curve of pure and technical grade yeast until the 4th subculture generation. Furthermore, we used both grades of yeast for elicitation. Kaffir lime cell suspension was treated with 10 ppm pure grade or 5 ppm and 10 ppm technical grade yeast for 4 days. After elicitation, kaffir lime cell lines were subcultured and their growth was analyzed. The result showed that the morphology and growth curve of technical grade until 4th subculture generations was similar to the pure grade. On the other hand, after elicitation using pure and technical grade yeast and being subcultured, the growth of the elicitated kaffir lime cell line had the same pattern as the control group, but the cell density of the control group was higher than the elicitated group. The initial stationary phase of kaffir lime cell line was on the 17th day which is the best time to subculture. The subculturing process is important to maintain the viability of the kaffir lime cell line.

Active Fractions of Black Rice Bran cv Cempo Ireng Inducing Apoptosis and S-phase Cell Cycle Arrest in T47D Breast Cancer Cells Rarastoeti Pratiwi; Alfi Rizqi Amalia; Woro Anindito Sri Tunjung; Rumiyati Rumiyati
Journal of Mathematical and Fundamental Sciences Vol. 51 No. 1 (2019)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2019.51.1.4

Indonesian black rice cv Cempo Ireng was evaluated for cancer prevention using a T47D cell line model. Methanolic extract of black rice bran (BRB) showed cytotoxicity with an IC50 of 522.13 µg/ml. This result was in contrast to water extract of BRB, which yielded an IC50 of more than 6000 µg/ml. From the methanolic extract of BRB, its 6 fractions were found from the preparative TLC performance, with Rf values ranging from 0 to 0.97. According to the MTT assay of all fractions, fraction 3 (F3) had the lowest IC50 (60.17 ± 1.72µg/ml), while fraction 2 (F2) had an IC50 of 64.3 ± 0.61µg/ml. However, F2 showed the highest induction of apoptosis, i.e. about 75.39 ± 0.43% compared to the T47D cells without treatment (control) (about 8.39 ± 0.16%) and with doxorubicin treatment (about 41.30 ± 0.08%). Furthermore, fraction 5 (F5) not only induced apoptosis but also increased the S-phase arrest percentage (60.36 ± 2.07%), which was significantly higher than for the other treatments. Therefore, each fraction of BRB showed different cytotoxic responses to T47D cells.

Effect of diazepam on kidney function and histological structure of white rat’s kidney Putu Gede Martha Setiawan; Woro Anindito Sri Tunjung; Luthfi Nurhidayat
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

The use of NAPZA (Narcotics, Alcohol, Psychotropic, and other addictive substances) in Indonesia keeps increasing. One type of frequently used NAPZAs is diazepam. Diazepam is a kind of sedative-hypnotics drug which belongs to benzodiazepine. The objective of this study was to determine kidney function by examining the level of urine ureum and creatinine also histological structure in rat after treated with diazepam. Rats were divided into one control group and three diazepam treatment groups which were solvent control (PEG 1%), treatment I (62.25 mg/kg BW), treatment II (83 mg/kg BW), and treatment III (124.5 mg/kg BW) of diazepam. These doses were based on LD50 in human. Rat urine was taken on the D0, D7, D14, D21, and D28. The ureum level was examined by ureumse-GLDH method and the creatinine level was analyzed by Jaffe method. The histological section was made by paraffin method with Hematoxylin and Eosin (HE) staining. Results showed that diazepam given to rat for 28 days led the in-crease of urine ureum and creatinine levels compare to control group. Furthermore, histological damages of the kidney also detected including cellular injuries, both reversible and irreversible injuries, congestion, hemorrhage, and glomerular damage. In conclusion, the use of diazepam for 28 days affected kidney function in rat.

Mekanisme Penyakit Kardiovaskular terkait Penuaan Nuriliani, Ardaning; Apriliyani, Tia; Pusparini, Nur Ainun Oktavia; Karmilah, Karmilah; Rohmah, Zuliyati; Tunjung, Woro Anindito Sri
Bioma : Berkala Ilmiah Biologi Vol. 26 No 2, Tahun 2024
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2024.59115

Penuaan merupakan proses patofisiologis ditandai dengan penurunan fungsi sel dan jaringan yang menyebabkan peningkatan risiko penyakit terkait penuaan, salah satunya penyakit kardiovaskular. Kajian pustaka ini bertujuan untuk mempelajari mekanisme beberapa penyakit kardiovaskular seperti hipertensi, aritmia, dan stroke. Data kajian berupa data sekunder yang berasal dari jurnal dan artikel tanpa batasan tahun terbit. Basis data yang digunakan yaitu Science direct, google scholar, NCBI, scopus, dan web. Kata kunci yang digunakan untuk pencarian buku, jurnal, dan artikel adalah hipertensi, hypertension mechanism, hypertension factor, stroke diseases, stroke mechanisms, risk factor causes stroke, stroke molecular mechanisms, arrhythmia, arrhythmia disease, arrhythmia mechanism, risk factor of arrhythmia, risk factor of arrhythmia. Hipertensi disebabkan oleh ROS yang mengikat nitrat oksida (NO) menghasilkan peroksinitrit (ONOO2) sehingga terjadi disfungsi endotel. Aritmia terjadi akibat kondisi inflamasi yang mengaktivasi oksidase NADPH menyebabkan perkembangan fibrilasi atrium kronis. Stroke terjadi akibat penyempitan pembuluh darah yang menyebabkan akumulasi sel senesen dan menginduksi peradangan serta penuaan endotel. Penyakit kardiovaskular seperti hipertensi, aritmia, dan stroke dapat disebabkan oleh mekanisme yang berkaitan dengan penuaan.

Validasi Metode Analisa Penetapan Kadar Glukosa dengan Metode Dubois secara Spektrofotometri Rahmayani, Almira; Marzuki, Ahmad; Ritmaleni, Ritmaleni; Sri Tunjung, Woro Anindito
Majalah Farmaseutik Vol 20, No 4 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/farmaseutik.v20i4.97119

Penetapan kadar glukosa dalam sampel biologis dan makanan sangat penting di berbagai bidanhg khususnya farmasi dan makanan. Ketidakseimbangan kadar glukosa dalam darah dapat menjadi indikator berbagai kondisi kesehatan seperti diabetes mellitus. Penelitian ini bertujuan untuk memvalidasi penetapan kadar glukosa dengan metode Dubois dengan spektrofotometri. Metode ini melibatkan reaksi antara glukosa dengan fenol dan asam sulfat pekat, menghasilkan senyawa berwarna kuning yang dapat terbaca pada panjang gelombang visibel ± 490 nm. Proses validasi mencakup uji spesifisitas, linearitas, LOD-LOQ, akurasi, dan presisi sesuai pedoman ICH Q2(R1). Hasil validasi menunjukkan spesifisitas pada l 488 nm dengan nilai R = 0,999, LOD serta LOQ pada 13,36 ppm dan 44,53 ppm, % perolehan kembali 101,11% dan RSD 1,73%. Berdasarkan parameter validasi yang telah diuji, maka prosedur penetapan kadar glukosa dengan instrumen spektrofotometri telah memenuhi kriteria dan dinyatakan telah valid.

Co-Authors Ahmad Marzuki Alfi Rizqi Amalia Alfi Rizqi Amalia Alisa Julia Nurulita Apriliyani, Tia Ardaning Nuriliani Aries Bagus Sasongko Christy, Ghea Putri Dewi Yuliana Rizqi Endang Semiarti Fatonah, Vita Fhea Putri Cristy Frisca Damayanti Ghea Putri Christy Karmilah, Karmilah Lisna Hidayati Nurhidayat, Luthfi Pratiwi Apridamayanti Pusparini, Nur Ainun Oktavia Putu Gede Martha Setiawan R. Rumiyati Radhiyah Mardhiyah Hamid Rahmayani, Almira Rarastoeti Pratiwi Rarastoeti Pratiwi Rifqi Zahroh Janatunaim Ritmaleni, Ritmaleni Rohmah, Zuliyati Rumiyati Rumiyati Rumiyati Rumiyati Triono, Sugeng Yekti Asih Purwestri

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