Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Kedokteran Hewan
Darmawan, I Wayan Angga
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
PENGARUH MALTOSA SEBAGAI KRIOPROTEKTAN EKSTRASELULER DALAM MENINGKATKAN KUALITAS SEMEN BEKU GUNA MENDUKUNG KEBERHASILAN TEKNOLOGI INSEMINASI BUATAN herdis, herdis; darmawan, i wayan angga
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.82 KB) | DOI: 10.29122/jsti.v14i3.926

Abstract

The research carried out to observe the effect of maltose addition on the quality of frozen semen of garut rams. Semen was collected once a week using artificial vagina from six mature garut rams. Semen was equilibrated at 5oC for four hours, frozen and stored in liquid nitrogen. The thawing was carried out at the temperature 30oC for 30 seconds. The result showed that percentages of viable sperm for addition of maltose 1,2 g / 100 ml extender (68,50 ± 0,84%) was significantly difference (P<0,05) than control (54,83 ± 1,94%) and addition of maltose 0,6 g / 100 ml extender (65,67 ± 1,03 %). The percentages of progressive motile sperm and percentages of plasma membrane for addition of maltose 1,2 g / 100 ml extender (53% and 64,67%) were significantly difference (P<0,05) than control (43% and 53,83%) but were not significantly different (P>0,05) from addition of maltose 0,6 g / 100 ml extender (50,83% and 64,67%)respectively. In conclusion, the addition of maltose 1,2 g / 100 ml extender is optimal dose to improve the quality of frozen semen of garut rams.
PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) Herdis, Herdis; Darmawan, I Wayan Angga; Rizal, Muhammad
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5139

Abstract

ABSTRACT The purpose of this research was to examine the effect of various sugars addition in Tris extender on quality of frozen thawed epididymal spermatozoa of ram. Collected spermatozoa was divided into five tubes and centrifuged at 3,000 rpm for 30 minutes. Supernatants were removed and pellets were diluted with Tris extender (control), Tris extender + 0.4% dextrose (dextrose), Tris extender + 0.4% maltose (maltose), Tris extender + 0.4% lactose (lactose), and Tris extender + 0.4% sucrose (sucrose), respectively. There were no significantly difference among treatment on mean percentages of motile (MS), live (LS) and intact plasma membrane (IPM) after equilibration. Mean percentages of post-thawed MS, LS, and IPM for dextrose (43.0, 53.8, and 53.4%), maltose (44.0, 54.8, and 52.8%), lactose (44.0, 52.8, and 52.8%), and sucrose (44.0, 55.0, and 53.4%) were significantly (P0.05) higher than control (37.0, 44.0, and 40.8%). In conclusion, addition of 0.4% dextrose or 0.4% maltose or 0.4% lactose or 0.4% sucrose in Tris extender increased quality of frozen thawed cauda epididymal spermatozoa of ram.
Co-Authors herdis herdis MUHAMMAD RIZAL