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The Role of Antioxidant for Improving The Quality of Frozen Semen Rizal, Muhammad; ., Herdis
Indonesian Bulletin of Animal and Veterinary Sciences Vol 20, No 3 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.838 KB) | DOI: 10.14334/wartazoa.v20i3.937

Abstract

The quality of frozen semen will be reduced if high oxidative metabolism activity in semen occurs, due to the formation of free radical compounds causing lipid peroxidation reaction on sperm plasma membrane. Lipid peroxidation occurs due to the exposure of semen to the oxygen during handling. Lipid peroxidation can be prevented by the addition of antioxidant compounds such as vitamin C, vitamin E, glutathione, and b-carotene, in the semen extender. Results of some researches showed that the addition of various antioxidans in extender can improve the quality of frozen semen of various animals. Key words: Antioxidant, free radical, lipid peroxidation, frozen semen
Utilization of Epididymal Sperm in Reproductive Technology Rizal, Muhammad; ., Nasrullah
Indonesian Bulletin of Animal and Veterinary Sciences Vol 14, No 1 (2004)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (767.619 KB) | DOI: 10.14334/wartazoa.v14i1.812

Abstract

Cauda epididymal sperm could be used as an alternative of male gamete source for application in reproductive technology, because the sperm is motile and has ability for fertilizing oocyte. This sperm could be collected from life or death animal, then it might be processed for chilling or freezing. Epididymis from death animal could also be preserved at 5°C for several days before collecting and processing. Cauda epididymal sperm which has motility more than 40% can be applied for artificial insemination (AI) or in vitro fertilization (IVF) programs. Key words: Cauda epididymal spermatozoa, Al, IVF
Quality of Garut ram frozen semen in various glycerol concentrations Rizal, Muhammad; Toelihere, M.R; Yusuf, T.L; Purwantara, B; Situmorang, P
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 3 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.127 KB) | DOI: 10.14334/jitv.v7i3.294

Abstract

Semen was collected once a week using artificial vagina from four mature Garut rams. Immediately after initial evaluation, semen was divided into three parts and diluted with tris extender containing 3% (G3), 5% (G5), and 7% (G7) glycerol, respectively, each with the concentration of 100 million motile sperm 0.25 ml-1. Semen was loaded in 0.25 ml mini straws, and equilibrated at 50C for three hours, then frozen and stored in liquid nitrogen container. Results indicated that percentages of post thawing motility and live sperm for G5 (40 and 50.50%) were significantly higher than G3 (32.50 and 45.33%) (P<0.05), but not significantly different with G7 (39.17 and 47.67%) (P>0.05). Percentages of post thawing intact acrosomal and plasma membrane for G5 (42.67 and 43.17%) were significantly higher than G3 (36.17 and 38.17%) (P<0.05), but not significantly different with G7 (38 and 39.83%) (P>0.05). In conclusion, concentration of 5% glycerol is the optimal dose in maintaining frozen semen quality of Garut rams.   Key words: Glycerol concentrations, frozen semen, Garut ram
Viability of Bali bull epididymal spermatozoa preserved at 3–5oC in Tris extender with different lactose concentrations Rizal, Muhammad
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.24 KB) | DOI: 10.14334/jitv.v14i2.355

Abstract

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, because the spermatozoa is motile and has ability for fertilizing the oocyte. The objective of this research was to examine the effectivity of lactose in maintaining viability of Bali bull epididymal spermatozoa preserved at 3–5oC. Five testis with epididymides of Bali bulls were obtained from slaughterhouse. Epididymal spermatozoa was collected by the combination of slicing, flushing and tissues pressure of cauda epididymides with physiological saline (0.9% NaCl). Collected-spermatozoa was divided in equal volume into three tubes and diluted with Tris extender containing 20% egg yolk (control), Tris extender + 0.3 g lactose/100 ml (L0.3), and Tris extender + 0.6 g lactose/100 ml (L0.6), respectively. Diluted-spermatozoa was stored in refrigerator at 3–5oC. Quality of diluted-spermatozoa including percentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) were evaluated every day during storage at 3–5oC for six days. Data were analyzed using completely randomized design with three treatments and five replications. Results of this study showed that mean spermatozoa concentration, percentage of MS, percentage of LS, percentage of abnormal spermatozoa, percentage of cytoplasmic droplet, and percentage of IPM of Bali bull fresh epididymal spermatozoa were 11,222.5 million cell/ml, 75, 86.75, 10.5, 14, and 86.75%, respectively. At day-7 storage, percentages of MS, LS, and IPM for L0.3 (39, 50.6, and 51.6%) and L0.6 (39, 51.4, and 51.8%) were significantly (P<0.05) higher than control (29, 41.8, and 42.4%). In conclusion, addition of lactose in Tris extender extended viability of Bali bull epididymal spermatozoa preserved at 3–5oC. Key Words: Lactose, Preservation, Epididymal Spermatozoa, Bali Bull
Characteristics of reproductive performance of Garut rams Rizal, Muhammad; Toelihere, M.R; Yusuf, T.L; Purwantara, B; Situmorang, P
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 2 (2003)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.46 KB) | DOI: 10.14334/jitv.v8i2.383

Abstract

Basic information on reproductive potency of Garut rams is necessary in order to identify the capacity of rams in producing chilled or frozen semen. Eight Garut rams (three to five years old) were used in this study. The male sexual behaviors were observed and semen was collected once a week using artificial vagina. Semen quality was evaluated and its potency to produce frozen semen was calculated. Results of this study indicated that first, second, and third ejaculations were at the 29, 87 and 176th seconds, respectively. Fresh semen volume, sperm concentration, motility, intact acrosomal cap, and intact plasma membrane were 0.99 ml, 3224 million/ml; 76.67; 86.13 and 87.73%, respectively. Protein value, fructose, vitamin C, vitamin E, sodiu, potassium, calcium, magnesium, phosphor, chloride, and mangan in seminal plasma of fresh semen were 4140, 180, 3.2, 24, 180, 117, 9, 6.12, 60, 104, and 5 mg/ml, respectively. Measurement of head length, width, and length of sperm tail were 6.59, 3.99, and 42.65 μm, respectively. Length and width measurement of right and left testes, and scrotal circumference were 12.71, 6.5, and 32.36 cm, respectively. Capacity of each Garut rams to produce frozen semen from three consecutive ejaculations are 35.88 mini straw with the cencentration of 200 million motile sperm per 0.25 ml.   Key words: Reproductive characteristics, Garut rams  
Role of various sugars in improving frozen semen quality of Garut ram Rizal, Muhammad; ., Herdis; Boediono, Arief; Aku, Achmad Selamet; ., Yulnawati
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.223 KB) | DOI: 10.14334/jitv.v11i2.516

Abstract

Ram spermatozoa are sensitive to extreme changes in temperature during the freeze-thawed process. The present study was conducted to examine the effects of addition of various sugars in Tris extender on sperm cryosurvival of Garut ram. Semen was collected using an artificial vagina from three mature rams once a week. Immediately after initial evaluation, semen was divided into five parts and diluted with Tris extender (control), Tris extender + 0.4% dextrose, Tris extender + 0.4% raffinose, Tris extender + 0.4% trehalose, and Tris extender + 0.4% sucrose, respectively. Semen was loaded in to 0.25 ml mini straw with the concentration of 200 million or 800 million motile spermatozoa per ml. Semen was equilibrated at 5oC for three hours, then frozen and stored in liquid nitrogen for seven days. Quality of processed-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), intact acrosome cap (IAC), and intact plasma membrane (IPM) were evaluated after dilution, equilibration, and thawing, respectively. Data were analyzed using completely randomized design with five treatments and six replicates. Means were compared significant difference test at 0.05 significant level. Results of this research showed that there was no significantly difference (P>0.05) between treatments for all sperm quality parameters after dilution and equilibration. Mean percentages of post thawing MS, LS, IAC, and IPM for dextrose (54.00; 68.00; 66.60, and 57.83%), raffinose (50.00; 64.33; 61.80, and 61.75%), trehalose (50.83; 65.67; 61.40 and 57.75%), and sucrose (49.00; 66.80; 58.50 and 58.50%) were significantly (P<0.05) higher than control (40.83; 52.67; 54.60, and 49.40%) respectively. In conclusion, addition of 0.4% dextrose, raffinose, trehalose or sucrose in Tris extender are effective in improving frozen semen quality of Garut ram. Key Words: Sugars, Tris extender, Frozen Semen Quality, Garut Ram
Role of seminal plasma in maintaining quality of ram epididymal spermatozoa preserved at low temperature (3–5oC) ., Herdis; Aku, Achmad Selamet; Rizal, Muhammad; Surachman, Maman
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.72 KB) | DOI: 10.14334/jitv.v11i4.539

Abstract

Cauda epididymal spermatozoa could be used as an alternative source of gamete in the application of various reproductive technologies, because the spermatozoa is motile and has ability of fertilizing the oocyte. The purpose of this research was to examine the effect of addition of seminal plasma on the quality of ram cauda epididymal spermatozoa preserved at 3–5oC. Collected-spermatozoa was divided into five parts then centrifuged at 3,000 rpm for 30 min and the supernatant was removed. One-fifth was diluted with Tris extender (Tris) and the remaining was added 0.5 ml ram seminal plasma then diluted with Tris extender (PS-Tris), 15% AndroMed + 85% distilled water (PS-AM15), 20% AndroMed + 80% distilled water (PS-AM20), and 25% AndroMed + 75% distilled water (PS-AM25), respectively. Quality of collected-spermatozoa including spermatozoa concentration (SC), percentages of motile spermatozoa (MS), live spermatozoa (LS), abnormal spermatozoa (AS), cytoplasmic droplet (CD), and intact plasma membrane (IPM) were evaluated. Percentages of MS, LS, and IPM of diluted-spermatozoa was evaluated every day during preserved at 3–5oC for three days. Results of this study showed that mean SC, MS, LS, AS, CD, and IPM of fresh spermatozoa were 11,660 million/ml, 65, 81, 7.6, 10.2, and 82.2%, respectively. Addition of seminal plasma in cauda epididymal spermatozoa prior to dilution could maintain the quality of spermatozoa during preserved at 3–5oC for three days. At day-4 of storage, percentages of MS, LS, and IPM for PS-Tris (43, 58, and 59.2%), PS-AM20 (40.5, 53.75, and 53.75%), and PS-AM25 (40, 54.8, and 55.2%) were significantly (P<0.05) higher than Tris (21, 34.8, and 33.6%) and PS-AM15 (20, 40, and 42.2%). In conclusion, addition of seminal plasma in ram cauda epididymal spermatozoa prior to dilution with Tris or 20 and 25% AndroMed extenders could maintain the quality of spermatozoa during preserved at 3–5oC for three days, and could be used for artificial insemination or in vitro embryo production programs. Key Words: Cauda Epididymal Spermatozoa, Seminal Plasma, Tris, AndroMed, Ram
Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3–5oC Rizal, Muhammad; ., Herdis; Surachman, Maman; Mesang-Nalley, W. Marlene
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.075 KB) | DOI: 10.14334/jitv.v13i1.591

Abstract

In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE) buck spermatozoa preserved at 3–5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tubes then centrifuged at 3,000 RPM for 30 min. Supernatant of the first tube was mixed again with Pasteur pipette (treatment A or control). Supernatant of the second tube was removed (treatment B or without seminal plasma). Supernatant of the third tube was removed and changed with Priangan ram seminal plasma in the same volume (treatment C). Semen was diluted with Tris extender containing 20% egg yolk and stored in refrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) was evaluated every day during storage at 3–5oC for three days. Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, spermatozoa abnormal, and IPM of PE buck fresh semen, respectively was 0.68 ml, cream, thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12% and 84%. At day-4 of storage, percentages of MS, LS, and IPM for treatment C (40, 52.2 and 51.6%) was significantly (P<0.05) higher than that of: treatment B (31, 44.8 and 45.2%) and treatment A (11, 15.6 and 14.8%). In conclusion, seminal plasma of Priangan ram could maintain the quality of PE buck semen preserved at 3–5oC for three days, and it prevent semen from coagulation. Key Words: Seminal Plasma, Priangan Ram, Spermatozoa Viability, PE Buck
Parameters Affecting the Extraction Process of Jatropha curcas Oil Using a Single Screw Extruder Siregar, Ali Nurrakhmad; Ghani, Jaharah A.; Che Haron, Che Hassan; Rizal, Muhammad
Journal of Engineering and Technological Sciences Vol 47, No 3 (2015)
Publisher : ITB Journal Publisher, LPPM ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (511.426 KB) | DOI: 10.5614/j.eng.technol.sci.2015.47.3.3

Abstract

The most commonly used technique to separate oil and cake from J. curcas seeds is mechanical extraction. It uses simple tools such as a piston and a screw extruder to produce high pressure, driven by hand or by engine. A single screw extruder has one screw rotating inside the barrel and materials simultaneously flow from the feed to the die zone. The highest oil yield can be obtained by a well-designed oil press as well as finding the optimum conditions for all parameters involved during the extraction process. The influence of the parameters in a single screw extruder was studied using finite element analysis and computational fluid dynamics simulation with ANSYS POLYFLOW. The research  focused on predicting  the velocity, pressure and shear rate in the metering section that influenced the screw rotational speed and mass flow rate. The obtained results revealed that increasing the screw rotational speed will increase the pressure, velocity and shear rate. Meanwhile, increasing the mass flow rate results in decreasing the pressure while the velocity and shear rate remain constant.
EKSISTENSI PRINSIP-PRINSIP HUKUM ISLAM TERHADAP PENGATURAN PERANG DALAM HUKUM HUMANITER INTERNASIONAL RIZAL, MUHAMMAD
Legal Opinion Vol 4, No 2 (2016)
Publisher : Legal Opinion

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penelitian ini bertujuan: untuk mengetahui Untuk mengetahui Konsp prinsip-prinsip hukum Islam yang berlaku dan Hukum Humaniter Internasional berkenaan Perang; Untuk mengetahui implementasi prinsip-prinsip hukum Islam yang berlaku dan Hukum Humaniter Internasional berkenaan Perang;. Ruang lingkup dalam penelitian ini adalah prinsip-prinsip Hukum Islam tentang pengaturan perang dalam Hukum Humaniter Internasional berkenaan metode perang, Obyek-obyek yang dilindungi, alat perang, perlindungan tawanan perang, perlindungan penduduk sipil dan perlindungan korban tewas; Metode penelitian, menggunakan pendekatan yuridis normatif yakni metode penelitian yang berorientasi kepada hukum yang yang mengatur peprinsip-prinsip Hukum Islam mengenai perang dalam Hukum Humaniter Internasional, Obyek-obyek yang dilindungi, alat perang, perlindungan tawanan perang, perlindungan penduduk sipil dan perlindungan korban tewas. Hal dimaksud, mengumpulkan data primer dan data sekunder, dan tertir berdasarkan obyek penelirtian. Data dimaksud, diolah secara deskriptip. Hasil penelitian ditemukan bahwa: (a) Kalau ditinjau dari segi yuridisnya, Sistim Hukum Islam dan hukum humaniter mempersoalkan terhadap perlindungan hak dan kewajiban terhadap penduduk sipil terhadap aturan-aturan mengenai cara menyelesaikan konflik dan akibat dari pada konflik itu sendiri, perlindungan tawanan perang dengan maksud bahwa seorang tawanan tidak boleh diperlakukan secara semena-mena, sedangkan kepada penduduk sipil ditetapkan larangan menjadikan mereka sebagai sasaran serangan; (b) Implementasi sistim Hukum Islam dan hukum humaniter dalam mengatur perlindungan hukum terhadap manusia akibat konflik bersenjata, yaitu adanya perlakuan yang wajar terhadap sesama manusia serta memberi hormat dan perlindungan (respect and protection) dalam artian bahwa unsur kemanusiaan harus diutamakan, sehingga mencegah kepada tindakan-tindakan yang berlebihan sebagai mana yang tertuang pada Konvensi Jenewa 1949 serta Protokol Tambahannya dengan Hukum Islam yang berasal dari Al-Qur’an dan Al-Hadits. Hal dimaksud, secara garis besar menetapkan larangan membunuh warga sipil yang tidak ikut peperangan, membunuh anak-anak, wanita serta larangan menghancurkan benda-benda budaya, tempat ibadah dan tempat kemakslahatan lainnya.   Kata Kunci : Hukum Islam, Humaniter Internsional, Konflik, Kemanusiaan
Co-Authors *, Suyuti ,, Sriwinar -, M Saleh A, Sriwahyuningsih Piu A.W., Jumiadi Abdul Karim Abdurachman, Iman Abrani Sulaiman Achmad, Dwi Isyana Afdhal Fuadi Afifah, A.Najiah Nurul Afniasari, Riza Afriana Agi Maulana, Tansyah Agusniar, Susi Ahmad Wijayanto Ahyuna Ahyuna, Ahyuna Aima Insana Akbar, Yuhaiyatul Akbardiansyah Akbardiansyah, Akbardiansyah Akhmad Fauzi Akhriana, Asmah Aksan, Muhammad Al Misbah, Iyan Al Mubarok, Ahmad Zakki AL-AZHAR AL-AZHAR Al-misbah, Iyan Alfarizi, Reyhan Alfarras, Bayu Alfiyah, Teti Ali Nurrakhmad Siregar, Ali Nurrakhmad Ali Sarong Ali, Mad Alimuddin, Hardiyanti Amaluddin Amaluddin, Amaluddin Amanda Amanda Aminuddin Kasim Aminuyati Amiruddin, M. Rudini Kurniawan Ananda Arifin, Camelia Andi Asvin Mahersatillah Suradi Andi Intan Purnamasari Andi Makkulawu Panyiwi Kessi Andi Saenong Andri Soemitra Anis Wahdi Anisah Anliza Rahmi, Amelia Annisa Aprilia Annisa Fitriana Annisa, Salma Anwar, Rahma Isartina Aos Kuswandi Aprizal Fikri Ar, Dhita Pratiwi Arbi Abdul Kahfi ARDIANSYAH ARDIANSYAH Ardianti, Dyah Ayu Ardila, Mesy Arief Boediono Arif Fadillah Arifin, Arham Arifin, Imamul Arifin, Suci Ramadhani Ariq, Muhammad Arismanto, Dimas Fahrul Putra Arismawati, Resti AS Aku, AS Asep Sopian Askar, Muhammad Ichfan Augustia, Annisa Elfina Awaliyah, Neneng Awan Setiawan Ayu, Wira Ayudhia Rachmawati Azhari Azhari Azis H, Abd Azka Rizkina Azzura B Purwantara B, Ceskakusumadewi Baehaqi Baharuddin Baharuddin Baharuddin, Megawaty Ceskakusumadewi Bahri, Abdul Halem Bahtiar, Akbar Bahtiyar, Saiul Balqis, Syafira Laras Bambang Sukarno Putra, Bambang Sukarno Barus, Dodi Berasa, Cukup Karina Bintal Amin Biyatmoko, d Budy Santoso Bulan, Ramayanty Che Hassan Che Haron Dahlan Dainuri, Muhamad Rifan Damayanti Damayati, Yuni Danie Indra Yama Danuaji, Muhammad Febryan Darmawan, I Wayan Angga Darwin Darwin Darwin Dasrul Dasrul Delyani, Rista Desferiansyah Dessy Yoswaty Dewa Atmaja, Diandra DEWI APRI ASTUTI Dewi B, Ceskakusuma Dewi Fithria Dewi, Aprianah Puspa Sari Dewi, Miswati Mailisa Kaila Dhafin, Ahmad Dhita Pratiwi AR Diana, Farah Dianshar, Rahmad Dika, Saran Dinda Bhawika Wimala Pastika Djafar, Imran Djuaeni, M. Napis DWI KURNIAWAN, WAHYU Dwitya, Yonadiah Dyah Rahayuning Perwitasari Dzulkifli, Muhamad Arham Edward Rizky Ahadian Edwarsyah Edwarsyah, Edwarsyah Edwin, Romy Suryaningrat Efendi, Ramdan Endang Syahriani Enik Sulistyowati Erijal, Erijal Erima, Derila Evi Anggraheni, Evi Fahmi, Melta Rini Faiz, Muhammad Alfin Faizal Faizal, Faizal Fajrianto, Wahyu Fajriati, Anisa Fakhrudin, Jaini Fakhrurrazi faradina, aulia Faridah, Linda Fathuddin Fathuddin, Fathuddin Fatiatun, Fatiatun fatimah Fatimah Fatimah, O. Fauziah Fauziah Fauziah, Nor Fazri Amir Febri Yulini, Febri Febriana, Sara Febriani , Julia Febriansyah, Risnendang Febriyandi, Febriyandi Firdasari Firdaus, Herlan Firhat First Wanita, First Fitri Anggriani Fitri, Hana Eka Fitriani Fitriani Fitriani, Dinda Ayu Friyuanita Lubis Ghazali Syamni Ghinannafsi, Daffa Aliyo Giovanni Oktavinanda Guntur Alting, Muhammad Habibah Habibah Habibul Umam Taqiuddin Hadian, Gloria Rida Hadis, Muhammad Sabirin Hafid, Anita Hafinuddin Hafinuddin Hafizh, Damar Hafriani Hairul Anam Hakim Muttaqim Halim, Peter Hartono Haliza, Alya Nur Hamdani Hamdat, Aminuddin Hamidi Hamidi Hana Eka Hanafiah Hanafiah, Hanafiah Hanapi, Khaerunnisa Hanapi, Sitti Harlina Handayani, Isna Galuh Handayani, Yovita Tri Hanin, Jilan Harahap, Khairunnisa Hardinandar, Fajrin Harlina, Sitti Hartati Tamti, Hartati Hasyrif SY Hayatun Nufus Hendra Saputra Hendrawan, Muhammad Habib Herawati, Tetty Herdis . herdis herdis Heriadi Heriadi, Heriadi Heriadi, Herman Heriansyah Heriawan, Dicky Herliani Herliani Hermawan, Sapto Hidayat, Cindy Meisya Putri Hidayat, Muhamad Syukron Huda, Ahmad Aly Husaini Husni Ibnu Sani, Mohamad Malik Ida Ayu Suci Idris, Mochammad Agus Ika Sumantri Ikhsanul Khairi Ilham Ilham Inayah, Safa Aulafi Indah, Indah Sulita Indani, Farinza Tiara Indra Suyoto Kurniawan Indri Hendarti Intan Chairun Nisa Iqbal Irfany, Mohammad Iqbal Surya Negara, Muhammad Irfannur Irfannur Irja, Mhd Irja, Mhd. Irmawaty, Irmawaty Irwin Syahri Cebro Ismail Sulaiman Ismail, Nuralamsyah Isnawati Isnawati Istiqlaliah Nurul Hidayati Isyfiani, Tera Sella Ita Athia Ita Rahmania Kusumawati Iwan Setiawan Iwan Setiawan izar, Shaflina Jaharah A. Ghani Johanis M, Andrew Ridow Johanis, Andrew Ridow Juanto, Juanto Julfikar, Julfikar JULIARINI, NI KADEK Jumsir, Jumsir Kamal, Kamal Kamil, Muhammad Ihsan Karimah, Asmanadia Izzatul Karimah, Karimah Kartika Rose Rachmadi KARTINI ERIANI Khaddafi, Khaer Muh. Khaeroni Khaeroni, Khaeroni Khairi Akbar, Ahmad Khairunnisa Kholidiah Siagian, Nur Krisnanto, Budhi Krisnanto, Budi Kurahmadan, Hidayat Kusuma Janata, Luckyta Kusumayadi, Dimas L.M Zainul , L.M Zainul Lambali, Suryadi Latif, Nuraida Lawalata, Izaac L D Layuk, Novita Sambo Lazuardi, Muhammad Lutfi leka lutpiatina, leka Liestiany, Elly Liutfi, Anugrah Lubis, Fahmi Sahil Lubis, Rievananda Amalia Luh Ade Yuanita Andriani Lupitasari, Florentina Bety Indah Luqmantoro, Luqmantoro Lusita Lutfi, Andi Luthvia, Luthvia Lyswiana Aphrodyanti M, Andrew Ridow Johanis M, Saripuddin M. Fahrul Husni M. Rasyidin M.R Toelihere Madyana Patasik Magfirah, Magfirah Maghfirah, Nur Mahari, Desiana Ade Mahdaniah, Nony Irma Makherta Sutadji, Ika Maman Surachman Mariati Hutabarat, Ayu Marsanda, Siti Marsha, Ifan Marzuki Marzuki Maulidiya Maya Itasari Megawaty, Megawaty Miftah Pratama, Muhammad Milia Wahyuni Siregar, Eka Mirnawati, Suci MISWAR MISWAR Mochamad Irlan Malik Muchsidin, Feronica Fungky Mufid, Muhammad Mufrizal, Rizki Muh. Ridwan Muhammad Agam Thahir Muhammad Agus Muljanto MUHAMMAD ALI Muhammad Ali Ramdhani Muhammad Arif Muhammad Arif Nasution Muhammad Fajri Muhammad Fuad Muhammad Furqan Rasyid Muhammad Hatta Muhammad Hidayat Ginanjar, Muhammad Hidayat Muhammad Indar Pramudi Muhammad Iqbal Muhammad Iqbal Muhammad Riyadhi Muhammad Rizal Muhammad Rusmin, Muhammad Muhammad Zaini Mujahid Mujahid, Mujahid Mukhtarida, Mukhtarida Muktaridha, Muktaridha Muliani Muliani Muliani Muliani, Muliani Mulya, Widya Munandar Munandar Mursalim Mursalim Mursyidin Mursyidin Musdalifah Musdalifah Mushaf, Mushaf Mustamiruddin Mutaqin, Zaenal Muthohharoh, Marhamah Muttaqin, Gusti Zainal Nafisi, Aura Najmawati Najmuddin Najmuddin Napis, Andi Ahmad Zahri Nasaruddin Nasaruddin Nasrullah . Nasution, Farida Hanum Ningsih, Sri Haslia Nisak, Zikratun Niswah, Afa Akmala Nita Rianti Parawansa, Ismaya Nopriadi Nopriadi Nordiana, Nordiana Nour, Muntaha Novi Sukma Drastiawati Nst, Asrindah Nuary, Henny Nur Aini Nur Asiah NURADILLA, SITI Nurdini, Fatwamanda Nurfiani, Sinta Nurhasan, Abdul Kodir Nurisma, Maulidiya Aulia nurlatifah, aeni Nurmasyitah Nursyam Andi Syarifuddin Nurul Huda Nurwijayanti Kusumaningrum Nyahu Rumbang Octavia, Ratna Dewi Okto Ivansyah P Situmorang Palestina, Firdaus Ayu Pananrang, Ayyub Daeng Pandu Kresnapati Pane, Kiki Candra Pangeran, Suheriyandi Pannyiwi, Tenri Panus, Panus Patasikv, Madyana Perrodin, David D. Piu , Sriwahyuningsih Piu, Sri Wahyuningsih Piu, Sriwahyuningsih Prasetya, Bagas Dwi Prastyo, Pulung Hendro Pratama, Fajar Oza Pratiwi AR, Dhita Pratiwi Putri, Aprillia Pratiwi, Dinda Tria Pulungan, Adilah Rahma Purnamasari, Mia Purwandani, Ledy Purwanto, Benang Pusaka, Semerdanta PUTRA HARYANTO, ALBERTUS EKA Putra, Teguh Dwi Putra, Umar Farauk Eka Putri Swastika, Putri Putri, Vivin Jenika Putri, Winarti Kartika Qadri, Muhammad Rachmadani, Nova Sri Raden Marsha Aulia Hakim Rahayu, Rosi Rahmah, Anania Rahman, Maulana Rahmat Bakri Rahmat Januar Noor Rahmat Rahmat Rahmatul Annisa, Ervina Ramadhany, Siti Aulia Ramanda, Adam Ramdani, Nasrul Ramdhani, Fitria Ramlah Ramlah Ramlah Ramlah Ramli Ranggayoni, Nanda Putri Rasmiati Rasyidin, Muhammad Ratih Dewi Dwiyanti Ratna Meisa Dai, Ratna Meisa Ratna Sari Sinaga, Dewi Ratnawati Ratnawati Ria Arifianti Riak Asie, Erina Riandani, Reza Ridho Abdilah, Muhammad Noval Rifani, Muhammad Ade Rifiater, Ucok Hasian Rika Astuti Rina Septiani, Rina Riskanita, Dinda Risna Risna, Risna Rita Kurnia Apindiati Rivai, Iskandar Rizal Fauzi Rizki Tri Wahyudi Rizqiana, Sista Robith Khoiril Umam Rochman, Chaerul Rohayati Arifin, Rohayati Rosalina Rosalina Rosnaini Daga Rosnizar, Rosnizar Roza Yusfiandayani Rozzi Kesuma Dinata Rudi Hermi Rusdi Rusdi Rusofi, Rusofi Safitri, Novia Safitri, Widya Sani Safna, Safna Salam, Karta Negara Saleh, M Samalam, Abdul Gafar Samsuddin Siregar Samsuddin, Sadly Samsul Bahri Sandi, Dian Kharisma Santika Sari SANTOSO SANTOSO Saputra, Febri Hidayat Saputra, Wahyuddin Saputra, Wanda Andres saputri, Nabila dwita Meifianti Sari, Ceni Febi Kurnia Sari, Nisa Sari, Restia Rahma Sari, Yelsi Afita Sarnidasari Sartika, Yustin Sasmita, Halida Sasmita, Khania Fazza SATRIANI, INDRA Seftiawan, Darwin Seknun, Maulana M.H Serly, Serly Setianingsih, Dias Setyoningsih, Erika Vivin SF Luthfie Arguby Purnomo SF. Lukfianka Sanjaya Purnama Shalihul Nasir, Muhammad Siboro, Impol Sidqi Ali Fauzilah Iyas, Ahdika Sigit PRASTOWO Sihombing, Audrey Brigita Demarlin Simangunsong, Judith Kl Metusala Sipahutar, Merry K Sitaresmi, Pradita Iustitia Siti Komariyah Siti Nurhasanah, Tia Sofian, M. Taufan Sri Mintarti Sri Wahyuni Sri Yanna ST. Aminah Dinayati Ghani Suardi Zain, Suardi Sudarman Sudarmin Sudarmin Sudaryana, Yayan Sugiarti, Endang Suhardi Sujatmiko Sujatmiko Sumarna, Lukman Sumerta Dana, I Kadek Hendra Sumiadi Sunardi Sunardi Supriyadi SURI AMILIA, SURI Suryana, Eka Pala Suryani Suryani Susi Kresnatita Sutrisno, Brilliant Sutrisno Suwandaru, Rachman Suyuti Suyuti Syabriyana, Maliya Syahra Chandra Kirana Syahrul Syahrul Syakri, Sila Abdullah Syamsuddin, Sadly Syani, Ahmad Syardiansah, Syardiansah Syihabuddin Syihabuddin T.L Yusuf Tampilang, Adrian Otniel Tamsir, Tamsir Tang, Jusman Tanjung, Afriansyah Tarigasa, Odilo Tarlis, Andi Tengku Putri Lindung Bulan, Tengku Putri Terfiadi, Sari Yulis Tetty Herawaty, Tetty Teuku Amarullah Teuku Azuar Rizal Thantawi, Tubagus Rifqy Thohiri, Roza Titik Efnita, Titik Trihantana, Rully Tutur Jatmiko Udink Aulia Ujirta, Randi Umhersuny, Brithnay Vensca Valencia Umi, Lita Permata Umrah, Alya Nahdatul Usman Usman Uswatun Hasanah Utami, Rizka Veithzal Rivai Zainal Verawaty Verawaty Verona, Naza Vonny Indah Sari Waluyo, Isnan Warni, Firma Wasis Wasis Wawan Saputra Widi, TriSatya Mastuti Widyastuti, Ragil Putri Wilem Musu Wilmientje Marlene Mesang Nalley Winar, Sri Winata, Hanafis Sastra Wirdan, Wirdan Wisda, Wisda Wulandari, Tiara Sahfitri Yal Robiansyah Yardiani, Windi Yudiansyah, Radinal Yulian Firmana Arifin Yuliana, Lina Yulnawati . Yuni Safitri, Novi Yunita, Tika Rahma Yuslil, Adima Zahratul Fitri Zahriyanti, Zahriyanti Zainul, L.M. Zainul Zaman, Akhmad Nidhomuz Zamani, R. Fairuz Zamri, Zamri Ziaul Maula, Ziaul Zulfan Zurba, Nabil Zuriat, Zuriat