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All Journal HAYATI Journal of Biosciences Jurnal Veteriner JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Ilmu Ternak Veteriner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
herdis herdis
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Veterinary Other

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The Effect of Phyto-Lecithin on Preservation and Cryopreservation of Semen: A Review Aku, AS; Sandiah, N; Sadsoeitoeboen, PD; Amin, R; Herdis, Herdis
ANIMAL PRODUCTION Vol 9, No 1 (2007): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Artificial insemination represents one of technologies in livestock reproduction that can be applied to cattle, sheep, goats and other livestock. Application of livestock reproduction technology includes artificial insemination to increase reproductive efficiency. Semen processing is one critical phase in an artificial insemination program. The use of animal origin ingredient for semen extenders, such as egg yolk and milk, presents a risk of microbial contamination, which lead to the search for alternatives. To increase standard of quality, researchers exploits phyto-lesitin for semen extender and the results showed no significant differences in motility, viability, and acrosomal status of spermatozoa with phyto-lesitin extender when compared to tris-egg yolk-containing extenders. (Animal Production 9(1): 49-52 (2007) Key Words : Phyto-Lechitin, preservation, cryopreservation, semen
Optimalization of Equilibration and Thawing Methode on Freezing Process of Garut Ram Semen (Ovis aries) Herdis, Herdis; Toelihere, MR; Supriatna, I; Purwantara, B; Adikara, RTS
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Generally, the problem of semen freezing process  is  cold shock effect and intracelullar change due to water release which is  related to  ice crystal formation. One factor to solve the problem is finding out optimal equilibration time and thawing method so there is only a little  damage spermatozoa during freezing process.  The research has been done  to explore  the  influence of  equilibration time and thawing methode  on  frozen semen quality of garut rams. The results shown that the mean of percentages of progressive motile sperm, percentages of viable sperm, percentages of plasma membrane and acrosomal intact on four hours equilibration (52.50% ;  62.33%; 57.17% and 56.42%)  were not significantly different  (P>0.05) from five hours equilibration (52.27%;  63.67%;  56.92% and 57.58 %)  and six hours equilibration (54.17%; 61.00%; 59.42% and 58.58%) respectively.  The percentages of progressive motile sperm, percentages of viable sperm,   percentages of plasma membrane and  acrosomal intact on the thawing method  on the temperature of 370C  for 30 seconds (53.33% ;  62.39%; 57.94% dan 58.61) were not significantly different  (P>0.05) from the thawing methode  on the temperature of 250C for 45 seconds (52.22% ; 62.28%; 57.72% dan 56.44) respectively.  The conclusion shown that  the treatment of four hours equilibration, five hours equilibration,   six hours equilibration and also  the treatment of thawing method  on the temperature of 370C   for 30 seconds and the thawing methode  on the temperature of 250C   for 45 seconds do not have effect on garut ram freezing semen quality. (Animal Production 7(2): 74-80 (2005) Key  Words : Equilibration, Thawing, Semen, Garut ram
Viabilility of Rams Epididymal Sperm after Preservation in Low Temperature (5oc) Rizal, M; Herdis, Herdis; Boediono, A
ANIMAL PRODUCTION Vol 6, No 1 (2004): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The purpose of this research was to evaluate the viability of ram epididymal sperm collected from fresh caudal epididymis (H-0) or after storage in low temperature (5oC, in refrigerator) for one (H-1), two (H-2), and three (H-3) days.  Collected sperm were diluted in modified Tris extender and they were preserved in refrigerator up to four days.  The viability of diluted sperm was evaluated daily base on motility and sperm live.  Results indicated that mean sperm concentration after sperm diluted with 0.05 ml Tris extender of caudal epididymis was 2745 million/ml. Sperm motility and percentage of live for H-0 (71.25% and 82.83%) and H-1 (70.00% and 79.17%) were significantly higher (P<0.05) than H-2 (61.25% and 69.83%) and H-3 (51.67% and 66.17%).  Percentages of sperm motility and live of diluted sperm and preserved in refrigerator for H-0 were significantly higher (P<0.05) than H-1, H-2, and H-3.  These results showed that epididymal sperm collected from caudal epididymis up to three days of preservation (without further storage of the diluted sperm) could be used for artificial insemination or in vitro fertilization programs.  Diluted sperm of H-0 and H-1 could be preserved in refrigerator for two days and H-2 for one day. (Animal Production 6(1): 30-36 (2004) Key Words: Epididymal Sperm, Viability, Rams
Karakteristik Semen Segar Domba Garut Tipe Laga pada Tiga Waktu Penampungan Semen Herdis, Herdis
ZOO INDONESIA Vol 26, No 1 (2017): Juli 2017
Publisher : Masyarakat Zoologi Indonesia

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Abstract

Penelitian dilakukan untuk mengetahui pengaruh waktu penampungan semen yang berbeda terhadap karakteristik semen segar domba garut tipe laga. Penelitian menggunakan tujuh ekor domba garut jantan. Penampungan semen dilakukan seminggu sekali menggunakan vagina buatan. Karakteristik semen segar yang diamati adalah warna, volume, kekenta-lan, keasaman, konsentrasi, abnormalitas, persentase motil, persentase hidup dan persentase membran plasma spermato-zoa. Waktu penampungan semen yang berbeda berpengaruh terhadap karakteristik semen segar yang dihasilkan. Waktu penampungan semen pukul 06.00 menghasilkan kualitas semen segar domba garut tipe laga paling baik berbeda nyata (p<0,05) dibandingkan waktu penampungan semen pukul 12.00 namun tidak berbeda nyata (p>0,05) dibandingkan dengan waktu penampungan semen pukul 09.00. Dari penelitian yang dilakukan disimpulkan bahwa perbedaan waktu berpengaruh terhadap suhu dan kelembaban ruangan. Guna mendapatkan kualitas semen segar yang lebih baik disarank-an waktu penampungan semen domba garut tipe laga dilakukan pada pukul 06.00-09.00.
APLIKASI TEKNOLOGI LASERPUNKTUR DALAM MENINGKATKAN LIBIDO PEJANTAN DOMBA GARUT (Ovis Aries) herdis, herdis
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.021 KB) | DOI: 10.29122/jsti.v12i1.847

Abstract

Libido can be measured by the length of time needed by the ram from teasing ewes to ejaculation. Low libido is one inefficient factor in reproductive process of sheep. The research was carried out to assess the application of laserpuncturetechnology for increasing libido of Garut rams. Results of the research showed that from three ejaculations observed, the ram which was treated with laserpuncture was more responsive in its libido than control. At the first ejaculation, the ram which was treated with laserpuncture needed 20.67 seconds and more responsive than control 35,40 seconds. At the second ejaculation, the laserpuncture treated needed 85 seconds to responsif than control 129,2 seconds. At the third ejaculation, the laserpuncture treated needed 198,6 seconds to responsif than control 209 seconds. In conclusion, laserpuncture at six acupoints in Garut rams can improve libido.
PENGARUH WAKTU PENAMPUNGAN SEMEN TERHADAP GERAKAN MASSA SPERMATOZOA DAN TINGKAH LAKU KOPULASI PEJANTAN DOMBA GARUT herdis, herdis
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 1 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.664 KB) | DOI: 10.29122/jsti.v14i1.903

Abstract

The study was carried out to observe the influence of semen collectiontime toward the copulation behavior and the movement of massspermatozoa of garut rams. The semen of seven Garut rams wascollected twice a week. It indicates that the difference of semen collectiontime causes the difference of air temperature. The semen collected at6:00 am showed better mass sperm movement than at 12:00. Thesemen collected at 06:00 am; 9:00 am and 12.00 am did not show anyeffect toward the preejaculation copulation behavior. Otherwise thesemen collected at 06:00 am delivered longer intromisi period than at12:00 am.
PENGARUH MALTOSA SEBAGAI KRIOPROTEKTAN EKSTRASELULER DALAM MENINGKATKAN KUALITAS SEMEN BEKU GUNA MENDUKUNG KEBERHASILAN TEKNOLOGI INSEMINASI BUATAN herdis, herdis; darmawan, i wayan angga
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (55.82 KB) | DOI: 10.29122/jsti.v14i3.926

Abstract

The research carried out to observe the effect of maltose addition on the quality of frozen semen of garut rams. Semen was collected once a week using artificial vagina from six mature garut rams. Semen was equilibrated at 5oC for four hours, frozen and stored in liquid nitrogen. The thawing was carried out at the temperature 30oC for 30 seconds. The result showed that percentages of viable sperm for addition of maltose 1,2 g / 100 ml extender (68,50 &plusmn; 0,84%) was significantly difference (P&lt;0,05) than control (54,83 &plusmn; 1,94%) and addition of maltose 0,6 g / 100 ml extender (65,67 &plusmn; 1,03 %). The percentages of progressive motile sperm and percentages of plasma membrane for addition of maltose 1,2 g / 100 ml extender (53% and 64,67%) were significantly difference (P&lt;0,05) than control (43% and 53,83%) but were not significantly different (P&gt;0,05) from addition of maltose 0,6 g / 100 ml extender (50,83% and 64,67%)respectively. In conclusion, the addition of maltose 1,2 g / 100 ml extender is optimal dose to improve the quality of frozen semen of garut rams.
PENGARUH PENAMBAHAN a-TOKOFEROL PADA MEDIA PENGENCER TRIS KUNING TELUR TERHADAP KUALITAS SEMEN CAIR DOMBA GARUT herdis, herdis; kusuma, ida; D, i wayan Angga
Jurnal Sains dan Teknologi Indonesia Vol. 11 No. 3 (2009)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (69.844 KB) | DOI: 10.29122/jsti.v11i3.836

Abstract

The study was conducted to know the influence of a- tocoferrol additional into the egg yolk tris extender medium to the liquid semen quality of &ldquo;Garut&rdquo; lamb (Ovis aries). Parameter which had been evaluated i.e. percentages of progressive motile sperm, percentages of viable sperm and percentages of plasma membrane. On the 4 th day evaluation showed that the addition of a- tocoferrol with the dose 0,04 g/100 ml into the egg yolk tris extender produced the highest percentages ofprogressive motile sperm, percentages of viable sperm and percentages of plasma membrane ( 49,0 &plusmn; 6,5%; 68,6 &plusmn; 4,2% and 59,3 &plusmn; 6,5% ) but there were not significantly different from control ( 45,5 &plusmn; 5%; 66,0 &plusmn; 4,6% and 56,2 &plusmn; 5,7% )and the addition of a- tocoferrol with the dose 0,02 g/100 ml ( 49,0 &plusmn; 6,5%; 66,6 &plusmn; 4,0% and 59,3 &plusmn; 2,8% ) respectively. In conclusion, the addition of a-tocoferrol in the egg yolk tris extender medium no significant effect on the liquid semen quality.
RESPON ESTRUS DOMBA GARUT BETINA PADA PERLAKUAN LASERPUNTUR DENGAN FASE REPRODUKSI YANG BERBEDA herdis, herdis
Jurnal Sains dan Teknologi Indonesia Vol. 13 No. 3 (2011)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.863 KB) | DOI: 10.29122/jsti.v13i3.892

Abstract

The research was carried out to assess the technology of laserpuncture for estrous synchronization of ewes in different phase reproduction. The results of the research showed that the treatment of laserpuncture at 17 reproduction accupoints during the luteal phase and at anytime perform 100% and 95% of oestrous, respectively. It indicates that the laserpunktur technology is capable to generates a synchronization of oestrus in ewes. There was no significant difference of oestrus response which observed from both of treatments at luteal phase or at any time. It is concluded that the laserpuncture technology is one of alternative technology for estrous synchronization beside hormone treatment.
Viability of Frozen-Thawed Epididymal Sperm of Garut Ram MUHAMMAD RIZAL; HERDIS HERDIS
HAYATI Journal of Biosciences Vol. 12 No. 2 (2005): June 2005
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.873 KB) | DOI: 10.4308/hjb.12.2.61

Abstract

Sperm collected from cauda epididymis is a source of male gametes. The purposes of this study was to evaluate an quality of frozen-thawed sperm of garut ram which was collected from cauda epididymis and cryopreserved with modified Tris extender, i.e: Tris extender (control, KT), Tris extender + 60 mM lactose (LS), and Tris extender + 60 mM lactose + 0.05% glutathione (GL). Quality of collected sperm including concentration, motility, live sperm, abnormality, cytoplasmic droplet, intact acrosomal cap (IAC), and intact plasma membrane (IPM) were evaluated. Results showed that mean of sperm concentration, percentages of motility, live sperm, abnormality, cytoplasmic droplet, IAC, and IPM of fresh epididymal sperm were 13,993.33 million/ml, 70.83, 82.83, 10.83, 8.5, 85.83, and 81.33%, respectively. Sperm quality after equilibration for LS and GL were significantly (P
Co-Authors A Boediono Anak Agung Gede Sugianthara Arief Boediono AS Aku, AS B Purwantara B Purwantara Cece Sumantri D, i wayan Angga Darmawan, I Wayan Angga I Arifiantini I Inounu I K Sutama I Supriatna Ida Kusuma Kusuma Kusuma M Riza M Rizal M Surachman Maman Surachman MR Toelihere, MR N Sandiah, N PD Sadsoeitoeboen, PD R Amin, R R. Iis Arifiantini RTS Adikara Santoso Santoso Siti Zubaidah YULNAWATI YULNAWATI