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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Jurnal Penelitian Inovatif Makara Journal of Health Research Indonesian Journal of Community Empowerment
Yahya, Arif
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Red Yeast Rice Protects Hepatocytes conditions of Rats Receiving High Fat Diet Wahyuningsih, Doti; Elyani, Helmin; Damayanti, Dini Sri; Yahya, Arif; Fadli, Muhammad Zainal
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.954 KB) | DOI: 10.11594/jtls.09.02.06

Abstract

Triglyceride (TG) is a simple and the main lipid of the daily diet. Nowadays, TG is emerging as an independent risk factor for cardiovascular disease, and increasing clinical data that indicate a high level of this simple lipid in serum may alert and play a role in liver impairment. Red yeast rice (RYR) reduces serum TG in human and animals. The rice is widely used as a natural inhibitor of HMG-CoA reductase to correct serum cholesterol level. Bioactive components of the rice are well known to have antioxidant properties. This study examined whether RYR protects hepatocytes by evaluating the serum AST-ALT, HDL-Cholesterol (HDL-C), TG levels, and the number of liver foam cells in hypertriglyceridemic rats. Twenty male Wistar rats were grouped into 5. Four groups received a high-fat diet (HFD), 40 g/animal/day for 60 days to induce hypertriglyceridemic condition. Along with the HFD treatment, three groups received 108, 54, and 27 mg/kgBW/day of RYR, respectively. Two other groups received standard and only HFD diet, respectively. Intracardiac blood was collected for measuring AST and ALT using AST or ALT activity assay Kit, respectively, serum TG and HDL-C by Enzymatic Caloric Test.  Hematoxylin-Eosin–stained 4µmm thick slices of liver tissues were prepared to count foam cell number by a light microscope with 400x magnificence. Data were analyzed using the Kruskal Wallis continued by the Mann Whitney U test. The p values of < 0.05 were considered to be significant. The present study found that 108, 54, and 27 mg/kgBW/day of RYR significantly decreased serum TG, HDL-C, AST, and ALT compared with those of hypertriglyceridemic rats receiving no RYR. The decreasing levels of the parameters were in relation to the doses of RYR. The doses of 108 and 54 mg/kgBW/day resulted in complete recovery of the liver tissues suffered from steatosis (p < 0.05). The RYR dose of 108 mg/KgBW/day completely corrects the serum HDL-C level. In conclusion, red yeast rice may have a potency to protect hepatocytes injuries due to hypertriglyceridemia.
Sodium Benzoate is Associated with Salmonella typhi Resistant to Chloramphenicol Fajar, Jonny K; Puspitasari, Retno A; Dewi, Ariani R; Yahya, Arif; Anand, Jay R
Makara Journal of Health Research Vol. 20, No. 1
Publisher : UI Scholars Hub

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Abstract

Background: There are many factors that govern growth and resistant of Salmonella typhi. A study had reported that the use of sodium benzoate caused antibiotic resistant. However, no study has directly evaluated the effect of sodium benzoate exposure on S. typhi sensitivity to chloramphenicol. The aim of this study was to evaluate the resistance or sensitivity of S. typhi to chloramphenicol after sodium benzoate exposure. Methods: The study was conducted in seven groups: three treatment groups (sodium benzoate insensitive S. typhi+8 µg/mL, 16 µg/mL, and 32 µg/mL of chloramphenicol), three positive control groups (sodium benzoate sensitive S. typhi+8 µg/mL, 16 µg/mL, and 32 µg/mL of chloramphenicol), and one negative control groups (sodium benzoate sensitive S. typhi+0 µg/mL of chloramphenicol). The effect of sodium benzoate exposure to S. typhi sensitivity to chloramphenicol was measured after 24 hours. Spearman test was used to analyzed this association. Results: In this study, we found that the average S. typhi growth in the treatment groups (A, B, C) was 445 CFU/mL, 385 CFU/mL, and 171 CFU/mL, respectively. While in the positive control group (D, E, F) was not obtained any S. typhi growth. Average S. typhi growth in the negative control group was 430 CFU/mL. Discussion: We found that sodium benzoate exposure inhibited S. typhi growth and affected S. typhi sensitivity to chloramphenicol (p < 0.05). In addition, we found that 32 µg/mL chloramphenicol had the highest mean difference value, so this showed that the dose 32 µg/mL of chloramphenicol had the best effectiveness of various treatment groups (p < 0.05). Conclusions: Sodium benzoate exposure can inhibit S. typhi growth and cause S. typhi resistant to chloramphenicol.
Strategi Kampanye Digital Partai Keadilan Sejahtera Menghadapi Pemilu 2024 Yahya, Arif; Febrianti, Diani; Rasyad, Irfan Fadli
Jurnal Penelitian Inovatif Vol 4 No 1 (2024): JUPIN Februari 2024
Publisher : CV Firmos

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54082/jupin.254

Abstract

Seiring perkembangan era digital, partai politik turut memanfaatkan media digital termasuk media sosial untuk meningkatkan dukungan dan suara mereka. Penggunaan media digital sebagai sarana kampanye dinilai cukup efektif untuk memenangkan suara terutama dari kalangan generasi millennial dan generasi Z yang akan suara mayoritas dalam Pemilu 2024. Partai politik menggunakan big data yang bersumber dari media sosial untuk menyusun kampanye digital. Partai Keadilan Sejahtera (PKS) sebagai salah satu peserta pemilu 2024 turut menggunakan media digital untuk kampanyenya. PKS perlu Menyusun strategi kampanye digital dengan tujuan untuk meningkatkan popularitas, elektabilitass dan akseptabilitas sehingga dapat memenangkan pemilu 2024. Dalam penelitian ini ada sejumlah rumusan masalah yang menjadi batasan masalah yang dibahas, diantaranya peran teknologi digital dalam membantu PKS dalam mengimplementasikan strategi kampanye untuk Pemilu 2024, strategi kampanye digital yang dan oleh PKS dalam menghadapi Pemilu 2024, dan dampak dari strategi kampanye digital PKS meningkatkan popularitas, elektabilitas dan akseptabilitas partai. Penelitian ini menggunakan pendekatan kualitatif yang mendeskripsikan tujuan dari penggunaan media digital sebagai sarana kampanye dalam pemilu 2024. Oleh karena itu, peneliti melakukan penelitian dengan menggunakan paradigma konstruktivisme, untuk memahami cara PKS dalam merancang strategi kampanye digital yang efektif. Kampanye digital dengan memanfaatkan media sosial, cukup efektif digunakan di kota besar seperti Jakarta, namun penggunaan kampanye digital belum cukup efektif di daerah karena sejumlah faktor seperti topografi wilayah yang tidak terjangkau akses internet. Untuk memaksimalkan dan melengkapi kamapnye tersebuat, maka PKS juga masih terus menggunakan kammpanye konvensional. Dengan menggabungkan strategi konvensional dan digital, PKS dapat meningkatkan popularitas dan peluangnya untuk terpilih pada Pemilu 2024.
WORKSHOP PEMBELAJARAN DIGITAL BAGI GURU SDN SAMPANGAGUNG 1, KEC. MOJOSARI, KAB MOJOKERTO Gunawan, Wawan; Rusmawati, Retno Danu; Fatirul, Achmad Noor; Wiyarno, Yoso; Yahya, Arif; Karyanto, Yunus; Prajitno, Sunyoto Hadi; Suharti, Suharti; Mastoah, Imas; Muhlason, Muhlason; Salasa, Isna Badarus
MAJU : Indonesian Journal of Community Empowerment Vol. 3 No. 1 (2026): MAJU : Indonesian Journal of Community Empowerment, January 2026
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v3i1.2073

Abstract

The development of information technology requires teachers to have adequate competence in designing and implementing digital-based learning. Teachers are not only required to master the teaching material but also be able to utilize technology as an effective learning media and resource. However, elementary school teachers still encounter obstacles in optimally integrating digital technology into the learning process. Based on these conditions, this Community Service activity was carried out in the form of a Digital Learning Workshop for teachers at SDN Sampangagung 1, Mojosari District, Mojokerto Regency. This activity aims to improve teachers' understanding and skills related to digital learning concepts, the use of digital media and learning resources, and the use of the internet, blogs, YouTube, and social media in learning. In addition, this activity also aims to equip teachers with the ability to design innovative and contextual digital learning in accordance with the needs of the community.The characteristics of elementary school students were examined. The participants in this activity were all teachers at SDN Sampangagung 1, Mojosari District, Mojokerto Regency. The workshop was conducted online, combining discussion and practical sessions. The results showed that the Digital Learning Workshop improved teachers' conceptual understanding and practical skills in utilizing learning technology. Overall, this community service activity was deemed successful, based on the achievement of participant targets, the implementation of the material, and the improvement of teachers' skills in mastering digital-based learning.
Comparison of DNA Extraction Methods Between Conventional, Kit, Alkali and Buffer-Only for PCR Amplification on Raw and Boiled Bovine and Porcine Meat Yahya, Arif; Firmansyah, Marindra; Arlisyah, Annisa; Risandiansyah, Rio
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1176.347 KB) | DOI: 10.21776/ub.jels.2017.007.02.09

Abstract

Detection of porcine contamination in food material by employing PCR techniques is integral in halal food confirmation. However, PCR is both costly and laborious, particularly in DNA isolation method. This study explores several different methods in DNA extraction for PCR amplification in bovine and porcine raw and boiled meat samples. Four methods for DNA extraction (conventional PCI method, DNA isolation kit, alkaline-based method, and a DNA lysis buffer-only from the same kit) was employed followed by PCR using primers from previous studies and compared for DNA quality and quantity (in six replicates) and PCR amplification on the best three DNA samples. This study shows that in all samples, the conventional method had the best DNA yield based on nanodrop measurement, followed by an alkali-based method, buffer-only method, and DNA isolation kit. Each method except lysis-buffer only had at least one sample with good DNA quality. Conventional and isolation kit showed reliable positive PCR detection for all porcine and bovine samples (92% positive). Using the alkaline-lysis method, DNA was amplified reliably on boiled meat samples (83% positive). Lysis-buffer-only method did not show consistent PCR amplification on the samples used (50% positive). The conclusion was that conventional PCI method and DNA isolation kit showed high reliability in PCR amplification of bovine and porcine meats, both raw and boiled. While high DNA yield was obtained using the alkaline-lysis method, PCR amplification was only successful on boiled samples. Lysis-buffer only method yielded in poor DNA quality and was not able to result in reliable DNA amplification.Keywords: DNA extraction methods, halal, Porcine food contaminants.
Co-Authors Anand, Jay R Arlisyah, Annisa Damayanti, Dini Sri Dewi, Ariani R Doti Wahyuningsih Elyani, Helmin Fadli, Muhammad Zainal Fajar, Jonny K Fatirul, Achmad Noor Febrianti, Diani Karyanto, Yunus Marindra Firmansyah Mastoah, Imas Muhlason, Muhlason Prajitno, Sunyoto Hadi Puspitasari, Retno A Rasyad, Irfan Fadli Risandiansyah, Rio Rusmawati, Retno Danu Salasa, Isna Badarus Suharti Wawan Gunawan Wiyarno, Yoso