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All Journal The Journal of Experimental Life Sciences (JELS) Mukhtabar: Journal of Medical Laboratory Technology
Al Adawiah, Putri Rabiah
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience

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Comparison of Carbol Fuchsin Concentration on the Results of Acid-Fast Bacteria Staining (AFB) Al Adawiah, Putri Rabiah; Basarang, Mujahidah; Rantung, Juneke Gabriella
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.691

Abstract

Background & Objective: Infections caused by the bacterium Mycobacterium tuberculosis are known as tuberculosis. (TB). The treatment process takes a long time because this bacillus bacteria is very strong. Compared to other parts of the human body, this bacteria more frequently attacks the lungs. Patients who test positive for acid-fast bacilli (AFB) are the cause of pulmonary tuberculosis (TB). The aim of this research is to determine how the concentration of Carbol Fuchsin differs in the results of Acid-Fast staining. (BTA). Method: This research was conducted in a laboratory and used purposive sampling techniques with the Ziehl-Neelsen staining method, resulting in 5 positive acid-fast bacilli (AFB) samples. Result: The results of this study indicate that the best concentration is 1.5% carbol fuchsin, so it can be concluded that the optimal staining concentration is 1.5% carbol fuchsin. Conclusion: Based on the research conducted, it was concluded that the higher the concentration of carbol fuchsin, the stronger it binds to the cell walls that have a lipid layer, thus being able to bind the red colour.
Effect of Place and Time Storage on the Quality of Tubers Amorphophallus muelleri Blume Al Adawiah, Putri Rabiah; Azrianingsih, Rodiyati; Mastuti, Retno
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (788.188 KB) | DOI: 10.21776/ub.jels.2019.009.01.06

Abstract

Porang tubers are plant commodities that physiologically susceptible to damage. Longtime storage can decrease the quality of tubers. Furthermore, the presence of fungi or bacteria makes tubers cannot be stored in a long time. Decreasing quality of Porang tuber became a problem because of the higher demand for porang tubers. This research was conducted to determine the suitable place and time storage of porang tubers. Therefore, farmers can control or reduce the decrease in tuber quality. Methods of this research were an observation of color change based on the Munsell Color System, odor observation, hardness observation using penetrometer, the rotten area of tuber was measured using Leaf Area Meter, shrinkage of tuber weight, the percentage of rotten and sprouted tubers, density, and moisture content by the oven method. The results of this study were analysed statistically by ANOVA test. The hardness of the tubers has a significant effect on the storage place (soil, rack, and floor) for period 14 weeks. Storage time has an effect on changes of the weight loss, moisture content and decay area. The longer the tuber stored the higher percentage of sprouted and rotten tubers. The color of porang tubers change and the odor in the part of the rotten tubers increase during the storage period in each storage place. Porang tubers should not be stored for more than two weeks and placed on a rack to minimize the damage of the tubers.Keywords: Physical change, porang tubers, storage time.
Co-Authors Basarang, Mujahidah Rantung, Juneke Gabriella Retno Mastuti Rodiyati Azrianingsih