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All Journal Buletin Farmatera JURNAL FARMASIMED (JFM) Sumatera Medical Journal PITIMAS: Journal of Community Engagement in Health
Sari, Mutiara Indah
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Health Professions Immunology & microbiology Medicine & Pharmacology

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Journal : JURNAL FARMASIMED (JFM)

 1 
EFEK EKSTRAK DAUN GAMBIR ( UNCARIA GAMBIR ROXB) TERHADAP TINGKAT STRESS OKSIDATIF DAN EKSPRESI SIRTUIN 3 PADA HIPOKAMPUS MENCIT BETINA MODEL PENUAAN YANG DIINDUKSI D-GALAKTOSA Sinaga, Inda MH; Sari, Mutiara Indah; Ichwan, Muhammad MH
Jurnal FARMASIMED (JFM) Vol 3 No 1 (2020): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i1.473

Abstract

The aging process must be experienced by all living things, there will be a decrease in the tissue ability to maintain its function so that pathological conditions occur. Some things can cause aging, Free radical factors are external and internal factors that can trigger aging. Induce of d-galactose continuously one of the factors that can increase oxidative stress. This study was design to evaluate the effect of gambir leaf extract on oxidative stress and expression of the sirtuin 3 gene in the hippocampus of female mice induced intraperitoneally by d-galactose 150 mg/kgbb. Researchers conducted a post test only control group study with a sample of 24 female mice aged 10 - 12 weeks with the inclusion criteria an average body weight of 25-35 grams, obtained from the same breeding place, maintained at the same place and time and never as research material. Mice were divided into 4 groups with 6 mice each , group (C) female mice induced only d-galactose 150 mg / kg and in sonde aquabidest 0,1%/body weight, mice induced by d-galactose and sonde gambir leaf extract at a dose of 100 mg / kg bw group (C1), group (C2) 200 mg/kgbw, and 400 mg/kgbw group (C3). Blood samples were collected to determine the increase in oxidative stress levels of malondealdehyde (MDA) through spectrophotometry, and hippocampal tissue collected for PCR method so that the expression of the sirtuin 3 gene can be seen. Increased levels of MDA are associated with increased oxidative stress. This study reported the results of MDA levels were not significant ( p value <0.05). The results of the PCR reported an increase in expression 2 times higher than the control at a dose of gambier leaf extract 200 mg / kgbw Keywords:D-Galactose, Malondealdehid,Sirtuin 3,Cathechin.
EFEK EKSTRAK DAUN GAMBIR ( UNCARIAGAMBIRROXB) TERHADAP TINGKAT STRESS OKSIDATIF DAN EKSPRESI BRAIN DERIVED NEUROTHROPIC FACTOR(BDNF) PADA HIPOKAMPUS MENCIT BETINA MODEL PENUAANYANG DIINDUKSI D-GALAKTOSA Sebayang, Julenda Irianti; Sari, Mutiara Indah; Ichwan, Muhammad Indah
Jurnal FARMASIMED (JFM) Vol 3 No 1 (2020): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i1.475

Abstract

Prevalence of premature aging that causes in decreased memory throughout the world of individuals aged 60 years and over is estimated at 5-7%. According to WHO this is caused by progressive dysfunction and neuronal cell death and is related to free radical formation. This study was design to evaluate the effect of gambir leaf extract ( uncariaGambirRocb ) on MDA and expression BDNF in the hippocampus of aging female mice that are induced in D-Galactose. In this study that method is used a post test only control group, the researcher design with 4 groups being treated ( group1 : inject D-Galactose 150 mg/kgbb/ip and sondeaquabidest 0,1%, group 2 : sondecathechins 100 mg and inject D-Galactose 150mg/kgbb, group 3 ; sondecathechins 200 mg and inject D-Galactose 150 mg/kgbb, group 4 : sondecathechine 400 mg and inject D-Galactose150 mg/kgbb) with the inclusion criteria : all female mice (MusMusculus L) were 10-12 weeks old and had not been studied. MDA is examined through blood serum with spektrofotometer. Isolation RNA with Kit Tiangen Kat DP419 and BDNF expression from the hippocampus by RT PCR Increased level of MDA showed increased stress oksidatif. This study the result of MDA levels were not significant ( p value <0.05 ). There was an increase in BDNF expression in the K2 group compared to the other group in the Control, but it was not significant. The dose of 200 mg/kgbb is determine as the most effective dose of gambier beverage to improve the memory of female mice.
Co-Authors Halimah, Windi Krisna Nur Ichwan, Muhammad Indah Ichwan, Muhammad MH Raditra, Ghinia Zati Hulwani Sanny Sanny Sebayang, Julenda Irianti Sinaga, Inda MH Yolahumaroh, Yolahumaroh Yulika, Tri Zafira, Nurul Izzah Zulaikha, Amanda Ratu